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        <gco:CharacterString>Methods and Sampling: &amp;lt;p&amp;gt;Methodology:&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Authors surveyed parrotfish assemblages, reef habitat, and predatory coral reef fishes at 82 sites across 31 reef structures on the Northern GBR spanning 6 degrees of latitude.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;br /&amp;gt;
Sampling and analytical procedures:&amp;lt;br /&amp;gt;
&amp;lt;br /&amp;gt;
Divers conducted a 40-minute timed swim at 6-10m depth while surveying fish and habitat using a diver-operated stereo video system. A second diver surveyed sharks, caragids, and other highly mobile reef predators on a 20m wide transect. A handheld GPS towed on a buoy enabled accurate reconstruction of survey paths and distances. Parrotfish were recorded to species and measured to nearest mm fork length (FL) using EventMeasure software. Parrotfish with FL &amp;amp;lt; 10cm were excluded. Divers also quantified environmental variables including reef slope, substrate rugosity, and live coral cover. Additional explanatory variables included predator biomass, geographic position (lat/lon), total reef area (ha), and management zone (according to GBRMPA 2003 zoning scheme).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Authors investigated changes in patterns of parrotfish community structure across the Northern GBR in relation to geographic and biophysical factors, as well as across management regimes, using redundancy analysis (RDA). RDA was performed using the “vegan” package in R version 3.5.1.&amp;lt;br /&amp;gt;
&amp;lt;br /&amp;gt;
Survey dives were conducted using SCUBA and a handheld stereo video system consisting of two Canon Legria underwater video cameras. Transects were measured using transect tape and towed GPS.&amp;lt;/p&amp;gt;</gco:CharacterString>
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The objective of this Research Coordination Network project is to develop an international network of researchers who use genetic methodologies to study the ecology and evolution of marine organisms in the Indo-Pacific to share data, ideas and methods. The tropical Indian and Pacific Oceans encompass the largest biogeographic region on the planet, the Indo-Pacific. It spans over half of the Earth's circumference and includes the exclusive economic zones of over 50 nations and territories. The Indo-Pacific is also home to our world's most diverse marine environments. The enormity and diversity of the Indo-Pacific poses tremendous logistical, political and financial obstacles to individual researchers and laboratories attempting to study the marine biology of the region. Genetic methods can provide invaluable information for our understanding of processes ranging from individual dispersal to the composition and assembly of entire marine communities.
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                            <gco:CharacterString>&lt;p&gt;&lt;em&gt;Project summary:&lt;/em&gt;&lt;br /&gt;
This research is designed to resolve the origins of Hawaiian reef fishes. All living inhabitants of the Hawaiian archipelago necessarily originate elsewhere, due to the volcanic history of the island arc. Hawaii also has the highest endemism (native species) in the Pacific, with 25% of the 625 near-shore fish species found nowhere else. Where did these fishes come from? Two prominent hypotheses regarding the origins of Hawaiian marine species maintain that colonists arrive either from the south (via the Line Islands and Johnston Atoll) or from the west (via Japan). Previous research has shown that Hawaiian endemic limpets (genus &lt;em&gt;Cellana&lt;/em&gt;) colonized from Japan (Bird et al. 2011 Mol. Ecol. 20:2128 – 2141). Andrews et al. (2014; PLoS One 9: e91665) report evidence for a colonization pathway from the south (Johnston Atoll) to the middle of the archipelago in the Papahanaumokuakea Marine National Monument (PMNM). In this project, we will sample locations to the south of Hawaii (Johnston and Line Islands) and to the west of Hawaii (Ogasawara and Ryukyu Islands) for a suite of 20 reef fishes in order to resolve the origins of Hawaiian biodiversity.  Advanced rebreather technology allows dives with longer bottom time and more efficient sample collection, and our program is pioneering the applications of this advance diving technology. To test alternate hypotheses in the lab, we will employ both population genetics (shifts in genotype frequencies) and phylogenetics (DNA sequence divergence) for more ancient separations. Restriction-digest associated DNA sequencing (RAD-seq) is the best method for studies of phylogeography, phylogenetics, and population biology because it provides high coverage of homologous portions of the genome from multiple individuals for comparatively low cost and effort. We use the ezRAD approach developed in the shared Bowen-Toonen Lab.&lt;/p&gt;
&lt;p&gt;&lt;strong&gt;Description from NSF award abstract:&lt;/strong&gt;&lt;br /&gt;
The Hawaiian Islands are the product of a volcanic hot spot in the middle of the North Pacific. Hence every living thing on this isolated archipelago has origins elsewhere. This project will investigate the origins of Hawaiian reef fishes, which are important both as a food source and a cultural touchstone in native Hawaiian communities. Two prominent hypotheses maintain that marine fish originally arrived from the south (Line Islands and Johnston Atoll) or from the west (Japan). To test these hypotheses, this research will augment existing specimens from Hawaii with expeditions to Johnston Atoll (closest shallow habitat to the south), the northern Line Islands (Palmyra), southern Line Islands (Christmas Island), and Ryukyu Islands and Ogasawara Islands in Japan. Advanced genetic techniques will be used to resolve the closest relatives to the Hawaiian fish species and the pathways by which reef species colonize Hawaii and help establish patterns of biodiversity. In cases where Hawaiian species are closely related to widespread sister species, this project will detect hotspots of genetic divergence. Because this research will reveal the sources of Hawaiian marine biodiversity, results can be used to help define priorities for reef protection. The project will support two graduate students and train at least two more in all aspects of the project from rebreather diving, specimen collection and curation, information management, and advanced genetic techniques. There will be outreach efforts to schools through existing programs, and expedition teams will include a videographer to provide footage for the award-winning Voice of the Sea program, broadcast locally. Expeditions will also include an outreach specialist to handle media reports and promote awareness and concern for reefs in the communities surrounding study sites. &lt;/p&gt;
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http://lod.bco-dmo.org/id/dataset-parameter/828727.rdf
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	Description: &lt;p&gt;slope of the reef substrate&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/828728.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/828730.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/828732.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/828733.rdf
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&amp;lt;p&amp;gt;&amp;lt;br /&amp;gt;
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&amp;lt;br /&amp;gt;
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&amp;lt;br /&amp;gt;
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