http://lod.bco-dmo.org/id/dataset/829025
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2020-11-16
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Series 1B-4: Multiple stressor experiments on T. pseudonana (CCMP1335) – Chlorophyll, particulate organic carbon and particulate organic nitrogen
2020-11-12
publication
2020-11-12
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-11-30
publication
https://doi.org/10.26008/1912/bco-dmo.829025.1
Uta Passow
University of California-Santa Barbara
principalInvestigator
Edward Laws
Louisiana State University College of the Coast and Environment
principalInvestigator
Julia Sweet
University of California-Santa Barbara
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Passow, U., Laws, E., Sweet, J. (2020) Series 1B-4: Multiple stressor experiments on T. pseudonana (CCMP1335) – Chlorophyll, particulate organic carbon and particulate organic nitrogen. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-11-12 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.829025.1 [access date]
Series 1B: POC, PON, Chl-a Dataset Description: Methods and Sampling: <p><strong>Experimental setup:</strong></p>
<p>The experiments were designed to test the combined effects of four temperatures, and eight light intensities on growth and photophysiology of the diatom T. pseudonana CCMP1335 in a multifactorial design. Four temperatures were tested: 15°C, 18°C, 22°C, and 26°C. Within each temperature, eight light levels were tested: 30, 40, 70,90,105,125,140 and 265 µmol photons · m-2 · s-1. All lights were set at a 12 h day: 12 h dark cycle. For logistical reasons, experiments were partially conducted in series.</p>
<p>Experiments were conducted in Multicultivator MC-1000 OD units (Photon Systems Instruments, Drasov, Czech Republic). Each unit consists of eight 85 ml test-tubes immersed in a thermostated water bath, each independently illuminated by an array of cool white LEDs set at specific intensity and timing. A 0.2µm filtered ambient air was bubbled through sterile artificial seawater, and the humidified air was supplied to each tube&nbsp; Each experiment was split into two phases: An acclimation phase spanning 3 days, was used to acclimate cultures to their new environment. Pre-acclimated, exponentially-growing cultures were then inoculated into fresh media and incubated through a 4-day experimental phase during which assessments of growth, photophysiology, and nutrient cycling were carried out daily. All sampling started 6 hours into the daily light cycle to minimize the effects of diurnal cycles.</p>
<p>Experiments were conducted with artificial seawater (ASW) prepared using previously described methods (Kester et. al 1967), and enriched with 50mL per liter of UV sterilized natural seawater and nitrate (NO3), phosphate (PO4), silicic acid (Si[OH]4), at levels ensuring that the cultures would remain nutrient-replete over the course of the experiment. Trace metals and vitamins were added as in f/2 (Guillard 1975). The pH of the growth media was measured spectrophotometrically using the m-cresol purple method (Dickson 1993), and adjusted using 0.1N HCl or 0.1M NaOH.</p>
<p><strong>Organic Carbon and Nitrogen concentrations</strong></p>
<p>Samples were filtered onto pre-combusted GF/F filters, dried at 60°C, and stored at room temperature until analyses of particulate organic carbon (POC), and particulate organic nitrogen (PON).&nbsp; Samples were analyzed using an elemental analyzer (CEC 44OHA; Control Equipment). Samples where C or N concentrations were below instrument detection limits (columns 'C_detection_limit_ug' and 'N_detection_limit_ug') were flagged (column 'Flags').</p>
<p><strong>Chlorophyll</strong></p>
<p>Daily subsamples from each treatment were filtered onto 0.45 µm polycarbonate filters and stored at -20°C. Filters were placed in 90% acetone (v/v) overnight at -20°C, and the extracted chlorophyll was measured fluorometrically on a Turner 700 fluorometer (Strickland 1972). Chlorophyll-a liquid standards in 90% acetone (Turner Designs Inc.), and adjustable solid secondary standards (Turner Designs Inc. P/N 8000-952) were used for calibrations, and to calculate the chlorophyll content of the samples (Column M)</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1538602 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1538602
completed
Uta Passow
University of California-Santa Barbara
709-864-8010
Ocean Sciences Centre, Memorial University Marine Lab Road, Logy Bay
St. John's
Newfoundland
A1C 5S7
Canada
uta.passow@mun.ca
pointOfContact
Edward Laws
Louisiana State University College of the Coast and Environment
225 578-3334
Louisiana State University School of the Coast and Environment 1002R Energy, Coast and Environment Building
Baton Rouge
LA
70803
USA
edlaws@lsu.edu
pointOfContact
Julia Sweet
University of California-Santa Barbara
Department of Biology
Lafayette
LA
70503
julia.sweet@lifesci.ucsb.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Phase
Temp
Day
Irradiance
Replicate
Reference_Label
C_ug
N_ug
Flags
C_detection_limit_ug
N_detection_limit_ug
Volume_filtered
Chl_a_ug_L
Multicultivator MC-1000 OD (Photon Systems Instruments, Drasov, Czech Republic)
Elemental analyzer (CEC 44OHA; Control Equipment)
Aquapen-C AP-C 100 (Photon Systems Instruments)
theme
None, User defined
sample description
water temperature at measurement depth
time_point
irradiance
replicate
sample identification
Elemental carbon (C)
Nitrogen
flag
volume of water filtered
chlorophyll a
featureType
BCO-DMO Standard Parameters
Cell Cultivator
CHN Elemental Analyzer
Fluorometer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Effects of multiple stressors on Marine Phytoplankton
https://osprey.bco-dmo.org/project/654347
Collaborative Research: Effects of multiple stressors on Marine Phytoplankton
<p>The overarching goal of this project is to develop a framework for understanding the response of phytoplankton to multiple environmental stresses. Marine phytoplankton, which are tiny algae, produce as much oxygen as terrestrial plants and provide food, directly or indirectly, to all marine animals. Their productivity is thus important both for global elemental cycles of oxygen and carbon, as well as for the productivity of the ocean. Globally the productivity of marine phytoplankton appears to be changing, but while we have some understanding of the response of phytoplankton to shifts in one environmental parameter at a time, like temperature, there is very little knowledge of their response to simultaneous changes in several parameters. Increased atmospheric carbon dioxide concentrations result in both ocean acidification and increased surface water temperatures. The latter in turn leads to greater ocean stratification and associated changes in light exposure and nutrient availability for the plankton. Recently it has become apparent that the response of phytoplankton to simultaneous changes in these growth parameters is not additive. For example, the effect of ocean acidification may be severe at one temperature-light combination and negligible at another. The researchers of this project will carry out experiments that will provide a theoretical understanding of the relevant interactions so that the impact of climate change on marine phytoplankton can be predicted in an informed way. This project will engage high schools students through training of a teacher and the development of a teaching unit. Undergraduate and graduate students will work directly on the research. A cartoon journalist will create a cartoon story on the research results to translate the findings to a broader general public audience.</p>
<p>Each phytoplankton species has the capability to acclimatize to changes in temperature, light, pCO2, and nutrient availability - at least within a finite range. However, the response of phytoplankton to multiple simultaneous stressors is frequently complex, because the effects on physiological responses are interactive. To date, no datasets exist for even a single species that could fully test the assumptions and implications of existing models of phytoplankton acclimation to multiple environmental stressors. The investigators will combine modeling analysis with laboratory experiments to investigate the combined influences of changes in pCO2, temperature, light, and nitrate availability on phytoplankton growth using cultures of open ocean and coastal diatom strains (Thalassiosira pseudonana) and an open ocean cyanobacteria species (Synechococcus sp.). The planned experiments represent ideal case studies of the complex and interactive effects of environmental conditions on organisms, and results will provide the basis for predictive modeling of the response of phytoplankton taxa to multiple environmental stresses.</p>
Stressors on Marine Phytoplankton
largerWorkCitation
project
eng; USA
biota
oceans
2018-11-17
2019-04-16
0
BCO-DMO catalogue of parameters from Series 1B-4: Multiple stressor experiments on T. pseudonana (CCMP1335) – Chlorophyll, particulate organic carbon and particulate organic nitrogen
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/829100.rdf
Name: Phase
Units: unitless
Description: <p>Indicates whether the sample was collected during the acclimation phase or the experiment phase of the experiment.</p>
http://lod.bco-dmo.org/id/dataset-parameter/829101.rdf
Name: Temp
Units: degrees Celsius
Description: <p>Indicates the temperature at which the samples were incubated</p>
http://lod.bco-dmo.org/id/dataset-parameter/829102.rdf
Name: Day
Units: unitless
Description: <p>Indicates the timepoint (day) of sampling. D0 = day 0; D1 = day 1; etc.</p>
http://lod.bco-dmo.org/id/dataset-parameter/829103.rdf
Name: Irradiance
Units: micromol photons/meter^2/second
Description: <p>Indicates the light intensity</p>
http://lod.bco-dmo.org/id/dataset-parameter/829104.rdf
Name: Replicate
Units: unitless
Description: <p>Indicates replication within a treatment</p>
http://lod.bco-dmo.org/id/dataset-parameter/829105.rdf
Name: Reference_Label
Units: unitless
Description: <p>Reference label (used internally for verifying sample identity)</p>
http://lod.bco-dmo.org/id/dataset-parameter/829106.rdf
Name: C_ug
Units: micrograms
Description: <p>Organic carbon concentration (in µg) in sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/829107.rdf
Name: N_ug
Units: micrograms
Description: <p>Organic Nitrogen concentration (in µg) in sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/829108.rdf
Name: Flags
Units: unitless
Description: <p>Indicates if C or N levels were below detection limits (DL) of the instrument</p>
http://lod.bco-dmo.org/id/dataset-parameter/829109.rdf
Name: C_detection_limit_ug
Units: micrograms
Description: <p>Instrument detection limit for organic carbon concentration (in µg)</p>
http://lod.bco-dmo.org/id/dataset-parameter/829110.rdf
Name: N_detection_limit_ug
Units: micrograms
Description: <p>Instrument detection limit for organic carbon concentration (in µg)</p>
http://lod.bco-dmo.org/id/dataset-parameter/829111.rdf
Name: Volume_filtered
Units: milliliters
Description: <p>Volume of the sample that was filtered</p>
http://lod.bco-dmo.org/id/dataset-parameter/829112.rdf
Name: Chl_a_ug_L
Units: micrograms/liter
Description: <p>Chlorophyll concentration</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
13973
https://darchive.mblwhoilibrary.org/bitstream/1912/26419/1/dataset-829025_series-1b-poc-pon-chl__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.829025.1
download
onLine
dataset
<p><strong>Experimental setup:</strong></p>
<p>The experiments were designed to test the combined effects of four temperatures, and eight light intensities on growth and photophysiology of the diatom T. pseudonana CCMP1335 in a multifactorial design. Four temperatures were tested: 15°C, 18°C, 22°C, and 26°C. Within each temperature, eight light levels were tested: 30, 40, 70,90,105,125,140 and 265 µmol photons · m-2 · s-1. All lights were set at a 12 h day: 12 h dark cycle. For logistical reasons, experiments were partially conducted in series.</p>
<p>Experiments were conducted in Multicultivator MC-1000 OD units (Photon Systems Instruments, Drasov, Czech Republic). Each unit consists of eight 85 ml test-tubes immersed in a thermostated water bath, each independently illuminated by an array of cool white LEDs set at specific intensity and timing. A 0.2µm filtered ambient air was bubbled through sterile artificial seawater, and the humidified air was supplied to each tube&nbsp; Each experiment was split into two phases: An acclimation phase spanning 3 days, was used to acclimate cultures to their new environment. Pre-acclimated, exponentially-growing cultures were then inoculated into fresh media and incubated through a 4-day experimental phase during which assessments of growth, photophysiology, and nutrient cycling were carried out daily. All sampling started 6 hours into the daily light cycle to minimize the effects of diurnal cycles.</p>
<p>Experiments were conducted with artificial seawater (ASW) prepared using previously described methods (Kester et. al 1967), and enriched with 50mL per liter of UV sterilized natural seawater and nitrate (NO3), phosphate (PO4), silicic acid (Si[OH]4), at levels ensuring that the cultures would remain nutrient-replete over the course of the experiment. Trace metals and vitamins were added as in f/2 (Guillard 1975). The pH of the growth media was measured spectrophotometrically using the m-cresol purple method (Dickson 1993), and adjusted using 0.1N HCl or 0.1M NaOH.</p>
<p><strong>Organic Carbon and Nitrogen concentrations</strong></p>
<p>Samples were filtered onto pre-combusted GF/F filters, dried at 60°C, and stored at room temperature until analyses of particulate organic carbon (POC), and particulate organic nitrogen (PON).&nbsp; Samples were analyzed using an elemental analyzer (CEC 44OHA; Control Equipment). Samples where C or N concentrations were below instrument detection limits (columns 'C_detection_limit_ug' and 'N_detection_limit_ug') were flagged (column 'Flags').</p>
<p><strong>Chlorophyll</strong></p>
<p>Daily subsamples from each treatment were filtered onto 0.45 µm polycarbonate filters and stored at -20°C. Filters were placed in 90% acetone (v/v) overnight at -20°C, and the extracted chlorophyll was measured fluorometrically on a Turner 700 fluorometer (Strickland 1972). Chlorophyll-a liquid standards in 90% acetone (Turner Designs Inc.), and adjustable solid secondary standards (Turner Designs Inc. P/N 8000-952) were used for calibrations, and to calculate the chlorophyll content of the samples (Column M)</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:</strong><br />
- &nbsp;data submitted in Excel file "BCODMO_Series 1B - 4_CHN_Chla_4Aug2020.xlsx" sheet "C N and Chl" extracted to csv<br />
- added conventional header with dataset name, PI name, version date<br />
- renamed columns to conform with BCO-DMO naming conventions (removed units and special characters)</p>
<p>&nbsp;</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Multicultivator MC-1000 OD (Photon Systems Instruments, Drasov, Czech Republic)
Multicultivator MC-1000 OD (Photon Systems Instruments, Drasov, Czech Republic)
PI Supplied Instrument Name: Multicultivator MC-1000 OD (Photon Systems Instruments, Drasov, Czech Republic) PI Supplied Instrument Description:Used for incubation of TP1014 cultures. Instrument Name: Cell Cultivator Instrument Short Name: Instrument Description: An instrument used for the purpose of culturing small cells such as algae or bacteria. May provide temperature and light control and bubbled gas introduction.
Elemental analyzer (CEC 44OHA; Control Equipment)
Elemental analyzer (CEC 44OHA; Control Equipment)
PI Supplied Instrument Name: Elemental analyzer (CEC 44OHA; Control Equipment) PI Supplied Instrument Description:Used for analysis of total organic carbon and nitrogen content. Instrument Name: CHN Elemental Analyzer Instrument Short Name:CHN_EA Instrument Description: A CHN Elemental Analyzer is used for the determination of carbon, hydrogen, and nitrogen content in organic and other types of materials, including solids, liquids, volatile, and viscous samples.
Aquapen-C AP-C 100 (Photon Systems Instruments)
Aquapen-C AP-C 100 (Photon Systems Instruments)
PI Supplied Instrument Name: Aquapen-C AP-C 100 (Photon Systems Instruments) PI Supplied Instrument Description:Used for assessment of photochemistry. Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/