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Experiments were conducted in the lab at the University of California Santa Barbara.<\/p>\n
Experimental setup:<\/strong><\/p>\n The experiments were designed to test the combined effects of four temperatures, and eight light intensities on growth and photophysiology of the diatom T. pseudonana CCMP1014 in a multifactorial design. Six temperatures were tested: 13.5\u00b0C, 20\u00b0C, 25\u00b0C, 29\u00b0C, 31\u00b0C, and 32.5\u00b0C. Within each temperature, eight light levels were tested: 25, 50, 80, 115,190, 300, 400 and 600 \u00b5mol photons \u00b7 m-2 \u00b7 s-1. All lights were set at a 12 h day: 12 h dark cycle. For logistical reasons, experiments were partially conducted in series.<\/p>\n Experiments were conducted in Multicultivator MC-1000 OD units (Photon Systems Instruments, Drasov, Czech Republic). Each unit consists of eight 85 ml test-tubes immersed in a thermostated water bath, each independently illuminated by an array of cool white LEDs set at specific intensity and timing. A 0.2\u00b5m filtered ambient air was bubbled through sterile artificial seawater, and the humidified air was supplied to each tube.\u00a0 Each experiment was split into two phases: An acclimation phase spanning 3 days, was used to acclimate cultures to their new environment. Pre-acclimated, exponentially-growing cultures were then inoculated into fresh media and incubated through a 4-day experimental phase during which assessments of growth, photophysiology, and nutrient cycling were carried out daily. All sampling started 6 hours into the daily light cycle to minimize effects of diurnal cycles.<\/p>\n Experiments were conducted with artificial seawater (ASW) prepared using previously described methods (Kester et. al 1967), and enriched with 50mL per liter of UV sterilized natural seawater and nitrate (NO3), phosphate (PO4), silicic acid (Si[OH]4), at levels ensuring that the cultures would remain nutrient-replete over the course of the experiment. Trace metals and vitamins were added as in f\/2 (Guillard 1975). The pH of the growth media was measured spectrophometrically using the m-cresol purple method (Dickson 1993), and adjusted using 0.1N HCl or 0.1M NaOH.<\/p>\n Flow cytometry:<\/strong><\/p>\n Samples were fixed in Hexamethylenetetramine-buffered formaldehyde (final concentration 1% v\/v) and stored at 4\u00b0C in the dark for a maximum of 4 days. Cell counts were confirmed to be unaffected over storage for up to a week. Samples were analyzed on a Guava easyCyte HT Benchtop Flow Cytometer (Millipore-Sigma, USA). All data acquisitions were done with logarithmic signal amplification. Cytometer sample flow rates were kept low (0.24 \u00b5L \u00b7 s-1) to accommodate high cell concentrations. Diatoms were identified based on size and chlorophyll autofluorescence using the forward scatter channel (FSC) and Red-FL (695\/50 nm) channel respectively. Growth rates were derived by fitting an exponential curve to cell concentrations vs. time for a 48-hour period during which cells exhibited exponential growth in the experimental phase. Growth rates in treatments where cells did not grow, or declined in abundance were listed as 0. Particle sizes (equivalent spherical diameter in \u00b5m, ESD) were derived from FSC using size-calibration beads of known diameters ranging from 2 \u00b5m to 10 \u00b5m (Particle Size standard kit, Spherotech Inc.).<\/p>\n Problem report:<\/strong>\u00a0 BCO-DMO Processing Notes:<\/strong>
\n- No data was collected from treatments grown at 32.5\u00b0C as this extreme temperature inhibited growth. Growth rate was zero.
\n-\u00a0Samples were lost from several treatments incubated at 20 and 25\u00b0C<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/ocean-data.org\/schema\/hasBriefDescription":[{"@value":"Series 2A: cell size, abundance","@language":"en-US"}],"http:\/\/www.w3.org\/2000\/01\/rdf-schema#label":[{"@value":"Series 2A: cell size and abundance","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/hasProcessingDescription":[{"@value":"
\n- data submitted in Excel file \"2a_cell_abund_size: BCODMO_Series 2A - 1_cellabund_size.xlsx\" sheets \"Abundance_ExptPhase\" and \"CellSize_ExptPhase1\" extracted to csv
\n- the two sheets were joined into a single table
\n- added conventional header with dataset name, PI name, version date
\n- renamed columns to conform with BCO-DMO naming conventions (removed units and special characters)<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/purl.org\/dc\/terms\/identifier":[{"@value":"829675","@type":"xsd:int"}],"http:\/\/purl.org\/dc\/terms\/title":[{"@value":"Series 2A: cell size and abundance"}],"http:\/\/purl.org\/dc\/terms\/date":[{"@value":"2020-11-18T10:32:33-05:00","@type":"xsd:dateTime"}],"http:\/\/purl.org\/dc\/terms\/created":[{"@value":"2020-11-18T10:32:33-05:00","@type":"xsd:dateTime"}],"http:\/\/purl.org\/dc\/terms\/modified":[{"@value":"2023-07-07T16:10:26-04:00","@type":"xsd:dateTime"}],"http:\/\/rdfs.org\/ns\/void#inDataset":[{"@id":"http:\/\/www.bco-dmo.org\/"}],"http:\/\/ocean-data.org\/schema\/namedGraph":[{"@value":"urn:bcodmo:dataset:829675","@type":"xsd:token"}],"http:\/\/ocean-data.org\/schema\/osprey_page":[{"@id":"https:\/\/www.bco-dmo.org\/dataset\/829675"}],"http:\/\/ocean-data.org\/schema\/identifier":[{"@value":"_:Identifier829675"}],"http:\/\/ocean-data.org\/schema\/datasetTitle":[{"@value":"Series 2A-1: Multiple stressor experiments on T. pseudonana (CCMP1014) \u2013 cell abundance and cell size in experiments","@language":"en-US"}],"http:\/\/ocean-data.org\/schema\/abstract":[{"@value":"These experiments were designed to test the combined effects of temperatures and light intensity on the growth growth rate (mu) and photophysiology of the diatom Thalassiosira pseudonana CCMP 1014 in a multifactorial design. Experiments were conducted in artificial seawater supplemented with 5% sterilized seawater. Six temperatures (13.5\u00b0C, 20\u00b0C, 25\u00b0C, 29\u00b0C, 31\u00b0C, and 32.5\u00b0C), and eight light intensities (25, 50, 80, 115,190, 300, 400 and 600 umol photons \u00b7 m-2 \u00b7 s-1 ) were tested during the course of these experiments. This dataset contains measurements of cell abundances and cell size expressed as forward scatter (FSC) as well as in equivalent spherical diameter (ESD) in microns.","@language":"en-US"}],"http:\/\/purl.org\/dc\/terms\/rights":[{"@id":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"http:\/\/ocean-data.org\/schema\/deprecated":[{"@value":"false","@type":"xsd:boolean"}],"http:\/\/ocean-data.org\/schema\/temporalExtent":[{"@value":"_:temporalExtent829675"}],"http:\/\/purl.org\/dc\/terms\/bibliographicCitation":[{"@value":"Passow, U., Laws, E., Sweet, J. (2020) Series 2A-1: Multiple stressor experiments on T. pseudonana (CCMP1014) \u2013 cell abundance and cell size in experiments. Biological and Chemical Oceanography Data Management Office (BCO-DMO). 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