http://lod.bco-dmo.org/id/dataset/834443
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2020-12-22
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Alexandrium cell counts from CTD bottle sampling from 2012 to 2019 in the Gulf of Maine
2021-05-04
publication
2021-05-04
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2021-05-04
publication
https://doi.org/10.26008/1912/bco-dmo.834443.1
Dennis J. McGillicuddy
Woods Hole Oceanographic Institution
principalInvestigator
Bruce Keafer
Woods Hole Oceanographic Institution
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: McGillicuddy, D. J., Keafer, B., Kosnyrev, O. (2021) Alexandrium cell counts from CTD bottle sampling from 2012 to 2019 in the Gulf of Maine. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-05-04 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.834443.1 [access date]
Alexandrium cell counts Dataset Description: Methods and Sampling: <p>Standard station CTD profiles measurements (down casts) with water sampling (up casts);</p>
<p>Calculations of the concentration (cells/Liter) of Alexandrium catenella, formerly A. fundyense, were completed using MS Excel. The spreadsheet used inputs of the original volume sieved at sea (usually 2 Liters), the volume re-suspended into formalin and then methanol (usually 14ml), and the volume of the plankton concentrate filtered for the molecular probe assay (usually 7ml,&nbsp; or less volume if during a significant bloom), and the number of labeled cells observed on that filter.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1131038 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1131038
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1314642 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1314642
Funding provided by National Institutes of Health (NIH) Award Number: NIH-P01ES021923 Award URL: https://reporter.nih.gov/project-details/8388954#details
Funding provided by National Institutes of Health (NIH) Award Number: NIH-P01ES028938 Award URL: https://taggs.hhs.gov/Detail/AwardDetail?arg_AwardNum=P01ES028938&arg_ProgOfficeCode=123
completed
Dennis J. McGillicuddy
Woods Hole Oceanographic Institution
508-289-2683
266 Woods Hole Road Bigelow 209B, Mail Stop 11
Woods Hole
MA
02543
USA
dmcgillicuddy@whoi.edu
pointOfContact
Bruce Keafer
Woods Hole Oceanographic Institution
+1 508 289-2509
Woods Hole Oceanographic Institution Department of Biology MS #32
Woods Hole
MA
02543
USA
bkeafer@whoi.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Year
Ship_ID
Cruise_number
Station
Depth
Live_cell_counts
Whole_cell_counts
Date_UTC
Time_UTC
Latitude
Longitude
ISO_DateTime_UTC
Sample_type
SeaBird 911
Rosette 24-position
Digiquartz
theme
None, User defined
year
ship
cruise id
station
depth
count
date
time of day
latitude
longitude
ISO_DateTime_UTC
sample description
featureType
BCO-DMO Standard Parameters
CTD Sea-Bird 911
Niskin bottle
Pressure Sensor
instrument
BCO-DMO Standard Instruments
CT2015-01
CT2015-04
CT2016-01
CT2016-02
CT2018-01
CT2018-02
CT2019-01
CT2019-02
GC2016-01
SI2017-01
WJ2017-01
TI603
TI606
TI667
TI670
TI672
TI691
TI747
TI751
TI758
TI813
TI817
TI831
TI906
TI972
TI978
service
Deployment Activity
Gulf of Maine
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Woods Hole Center for Oceans and Human Health
https://www2.whoi.edu/site/whcohh/
Woods Hole Center for Oceans and Human Health
Overall Abstract:
The Woods Hole Center for Oceans and Human Health (WHCOHH) will comprise a strong and integrated set of research projects using novel in situ sampling technologies and modeling approaches building on prior research to address how environmental factors influence harmful algal bloom (HAB) dynamics and human exposure to HAB toxins, a serious and global human health threat. The overall objective is to protect public health through enhanced understanding of how oceanic processes affect the intensity and distribution of toxin-producing HABs and to understand the potential health risks from exposure even to low levels of their potent neurotoxins, especially during susceptible stages of life. The Center will focus on two key HAB taxa: Alexandrium catenella, which produces the saxitoxins responsible for paralytic shellfish poisoning (PSP), and Pseudo-nitzschia spp., which produce domoic acid responsible for amnesic shellfish poisoning (ASP) syndrome, both are expanding geographically. Novel, targeted, efficient, and data-rich sampling approaches developed by the applicants and applied in situ in natural settings have revealed new controls of A. catenella population dynamics, and have identified possible new environmental links regarding toxic Pseudo-nitzschia species. Project 1 will examine further the physiological and environmental variables affecting these HABs, which may underlie population adaptation in different habitats and different environmental regimes. Project 2 will incorporate these new and fundamental insights on bloom regulation into coupled population models to predict HAB events, a key step toward being able to quantify future risks from this recurrent public health threat. In biomedical studies with the zebrafish model, Project 3 has identified myelination in the developing brain as a target of domoic acid. They will use transgenic zebrafish and single-cell RNA-sequencing to identify the cell-specific mechanisms underlying effects of domoic acid, saxitoxin, and the cyanotoxin anatoxin-a in zebrafish embryos in vivo and will use human iPSC-derived 3D brain systems in vitro to elucidate toxin effects on neural and glial cell differentiation in human cells. Studies also will determine whether prior exposure to ubiquitous persistent organic pollutants may modify effects of subsequent exposure to saxitoxin and domoic acid. All projects will collaborate to link oceanic processes to human exposure, helping to define the exposure of susceptible human subpopulations. The Community Engagement Core will facilitate integration of the research with education and engagement of resource managers and other stakeholders. We also will improve awareness of emerging HAB issues for the public health community and develop new educational materials and interactive activities for K-12 classrooms, and for health care providers. An Administrative Core will encourage open discussion of planning, integration, and communication, and provide rigorous evaluation of progress in all aspects of the program.
NSF Award Abstract: See https://www.nsf.gov/awardsearch/show-award?AWD_ID=2418297
The data management plan for the program can be found here.
WHCOHH
largerWorkCitation
program
WHCOHH - Physiological and behavioral plasticity in harmful algal bloom dynamics: variation across different habitats
https://osprey.bco-dmo.org/project/818414
WHCOHH - Physiological and behavioral plasticity in harmful algal bloom dynamics: variation across different habitats
<p><em>Alexandrium catenella</em> and <em>Pseudo-nitzschia</em> spp. threaten human health through their production of potent neurotoxins that respectively cause Paralytic Shellfish Poisoning and Amnesic Shellfish Poisoning. Both are widespread across coastal waters of the U.S. and globally. Environmental factors alter marine ecosystems in many unanticipated ways, and a major concern is that the occurrence of these harmful algal blooms (HABs) and their concomitant human health impacts will grow. This project addresses key uncertainties regarding the impact of environmental variability on the timing and severity of HABs through detailed biological and ecological study of <em>Alexandrium</em> and <em>Pseudo-nitzschia</em> blooms within the Gulf of Maine (GOM). The GOM is an ideal system because of the long history of regional HAB issues and associated groundbreaking research by WHCOHH. Comprehensive studies of bloom physiology, toxicity, and oceanographic drivers have led to construction of coupled physical-biological models that have improved our understanding of key HAB dynamics and set the stage for continued interdisciplinary investigation of mechanisms at the cellular-to-GOM scale that are relevant to human exposure and health. In particular, the physiological responses of these HABs — growth, toxicity, and life cycle transitions — to shifting environmental conditions remain uncertain. <strong>We hypothesize that long-term trends in HAB risks in the GOM are inherently linked to physiological processes and toxin dynamics that are driven by ecological stimuli</strong>. This project’s aims are designed to illuminate the role of life cycle dynamics and cellular physiology in determining the occurrence of <em>Alexandrium</em> and <em>Pseudo-nitzschia</em> blooms. We apply a wide variety of innovative approaches for study of natural populations in situ, leveraging a unique region-scale HAB observing system (HABON-NE). Aims 1 and 2 focus on <em>A. catenella</em> and seek to characterize cyst dormancy cycles, cyst formation, and subsequent bloom termination. These aims build upon prior successful approaches and models from study of inshore systems, asking the question if open water populations of the GOM are governed by similar dynamics. Aim 3 investigates the physiology underlying the emerging bloom dynamics of <em>Pseudo-nitzschia</em> in the GOM with in situ physiological observation coupled with targeted metatranscriptomics. Through the direct assessment of life cycle processes and physiology of HABs in the GOM, the results from this work will be directly applicable to the successful integration of physiology into models (Project 2) and inform estimates of potential risk for human exposure to HAB toxins (Project 3).</p>
WHCOHH Algal Bloom Dynamics
largerWorkCitation
project
eng; USA
oceans
Gulf of Maine
-70.7927
-66.113
41.5283
44.983
2012-05-04
2019-08-12
0
BCO-DMO catalogue of parameters from Alexandrium cell counts from CTD bottle sampling from 2012 to 2019 in the Gulf of Maine
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/836485.rdf
Name: Year
Units: unitless
Description: <p>Sampling year</p>
http://lod.bco-dmo.org/id/dataset-parameter/836486.rdf
Name: Ship_ID
Units: unitless
Description: <p>Vessel identifier: 1 - R/V Tioga, 2 - R/V Connecticut, 3 - R/V Gulf Challenger, 4 - R/V Warren Jr., 5 - R/V Scarlett Isabella</p>
http://lod.bco-dmo.org/id/dataset-parameter/836487.rdf
Name: Cruise_number
Units: unitless
Description: <p>Cruise identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/836488.rdf
Name: Station
Units: unitless
Description: <p>Station number</p>
http://lod.bco-dmo.org/id/dataset-parameter/836489.rdf
Name: Depth
Units: meters (m)
Description: <p>Nominal depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/836490.rdf
Name: Live_cell_counts
Units: cells per liter
Description: <p>live counts of cells concentration (live)</p>
http://lod.bco-dmo.org/id/dataset-parameter/836491.rdf
Name: Whole_cell_counts
Units: cells per liter
Description: <p>Alexandrium catenella cells concentration (WC)</p>
http://lod.bco-dmo.org/id/dataset-parameter/836492.rdf
Name: Date_UTC
Units: unitless
Description: <p>Date of data collection in UTC, standard ISO format (yyyy-mm-dd)</p>
http://lod.bco-dmo.org/id/dataset-parameter/836493.rdf
Name: Time_UTC
Units: unitless
Description: <p>Time of sample and data collection in UTC, standard ISO format (yyyy-mm-ddThh:mmZ)</p>
http://lod.bco-dmo.org/id/dataset-parameter/836494.rdf
Name: Latitude
Units: decimal degrees
Description: <p>latitude, south is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/836495.rdf
Name: Longitude
Units: decimal degrees
Description: <p>longitude, west is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/836496.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>Date and time of sample and data collection in UTC, standard ISO format (yyyy-mm-ddThh:mmZ)</p>
http://lod.bco-dmo.org/id/dataset-parameter/836497.rdf
Name: Sample_type
Units: unitless
Description: <p>Samples taken at ESP Stations, CTD stations or Underway</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
67110
https://darchive.mblwhoilibrary.org/bitstream/1912/27041/1/dataset-834443_alexandrium-cell-counts__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.834443.1
download
onLine
dataset
<p>Standard station CTD profiles measurements (down casts) with water sampling (up casts);</p>
<p>Calculations of the concentration (cells/Liter) of Alexandrium catenella, formerly A. fundyense, were completed using MS Excel. The spreadsheet used inputs of the original volume sieved at sea (usually 2 Liters), the volume re-suspended into formalin and then methanol (usually 14ml), and the volume of the plankton concentrate filtered for the molecular probe assay (usually 7ml,&nbsp; or less volume if during a significant bloom), and the number of labeled cells observed on that filter.</p>
Specified by the Principal Investigator(s)
<p>"Whole Cell" preserved samples. For the “Whole Cell” A. catenella water samples collected during the surveys, 2 Liters of seawater were drained from Niskin bottles into pre-rinsed bottles and sieved thru 20µm Nitex. The concentrated particulate material retained on the sieve was backwashed with filtered seawater (&lt;15µm) into 15ml centrifuge tubes to a final volume of 14ml and preserved with formalin to a final concentration of 5%. After storage at 4oC for no longer than 36 hours, the tubes were centrifuged for 5 min (5000 x g), formalin was removed by aspiration leaving the pellet, replaced with 100% cold methanol, and stored at -20C for later analysis.</p>
<p>Enumeratio"n of the WC samples followed the methods developed by Anderson et al (2005a) using a species-specific oligonucleotide probe (NA-1) conjugated to a Cy3 fluorochrome and visualized with epi-fluorescence microscopy.</p>
<p>“Live" Count samples. To obtain quick estimates of Alexandrium sp. concentrations while at sea, 10 L of seawater from a surface 10L Niskin bottle was sieved and concentrated to 14ml.&nbsp;&nbsp; A 1ml aliquot of the concentrate was loaded into a Sedgewick- Rafter counting chamber and enumerated using standard light microscopy.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
SeaBird 911
SeaBird 911
PI Supplied Instrument Name: SeaBird 911 PI Supplied Instrument Description:SeaBird 911+ Rosette 24-position, 10-liter bottle Rosette with dual T/C sensors At each station, CTD casts measured temperature, salinity and PAR. Water samples collected at depths of 300, 250, 200, 150, 120, 100, 80, 60, 40, 30, 20, 10 m, and the surface were filtered and preserved for nutrient analysis. Instrument Name: CTD Sea-Bird 911 Instrument Short Name:CTD SBE 911 Instrument Description: The Sea-Bird SBE 911 is a type of CTD instrument package. The SBE 911 includes the SBE 9 Underwater Unit and the SBE 11 Deck Unit (for real-time readout using conductive wire) for deployment from a vessel. The combination of the SBE 9 and SBE 11 is called a SBE 911. The SBE 9 uses Sea-Bird's standard modular temperature and conductivity sensors (SBE 3 and SBE 4). The SBE 9 CTD can be configured with auxiliary sensors to measure other parameters including dissolved oxygen, pH, turbidity, fluorescence, light (PAR), light transmission, etc.). More information from Sea-Bird Electronics. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0035/
Rosette 24-position
Rosette 24-position
PI Supplied Instrument Name: Rosette 24-position PI Supplied Instrument Description:SeaBird 911+ Rosette 24-position, 10-liter bottle Rosette with dual T/C sensors At each station, CTD casts measured temperature, salinity and PAR. Water samples collected at depths of 300, 250, 200, 150, 120, 100, 80, 60, 40, 30, 20, 10 m, and the surface were filtered and preserved for nutrient analysis. Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
Digiquartz
Digiquartz
PI Supplied Instrument Name: Digiquartz PI Supplied Instrument Description:A pressure sensor is a device used to measure absolute, differential, or gauge pressures. It is used only when detailed instrument documentation is not available. Instrument Name: Pressure Sensor Instrument Short Name:Pressure Sensor Instrument Description: A pressure sensor is a device used to measure absolute, differential, or gauge pressures. It is used only when detailed instrument documentation is not available.
Cruise: CT2015-01
CT2015-01
R/V Connecticut
R/V Connecticut
vessel
CT2015-01
Bruce Keafer
Woods Hole Oceanographic Institution
Cruise: CT2015-04
CT2015-04
R/V Connecticut
R/V Connecticut
vessel
CT2015-04
Bruce Keafer
Woods Hole Oceanographic Institution
Cruise: CT2016-01
CT2016-01
R/V Connecticut
R/V Connecticut
vessel
CT2016-01
Bruce Keafer
Woods Hole Oceanographic Institution
Cruise: CT2016-02
CT2016-02
R/V Connecticut
R/V Connecticut
vessel
CT2016-02
Bruce Keafer
Woods Hole Oceanographic Institution
Cruise: CT2018-01
CT2018-01
R/V Connecticut
R/V Connecticut
vessel
CT2018-01
Bruce Keafer
Woods Hole Oceanographic Institution
Cruise: CT2018-02
CT2018-02
R/V Connecticut
R/V Connecticut
vessel
CT2018-02
Bruce Keafer
Woods Hole Oceanographic Institution
Cruise: CT2019-01
CT2019-01
R/V Connecticut
R/V Connecticut
vessel
CT2019-01
Bruce Keafer
Woods Hole Oceanographic Institution
Cruise: CT2019-02
CT2019-02
R/V Connecticut
R/V Connecticut
vessel
CT2019-02
Bruce Keafer
Woods Hole Oceanographic Institution
Cruise: GC2016-01
GC2016-01
R/V Gulf Challenger
Community Standard Description
International Council for the Exploration of the Sea
R/V Gulf Challenger
vessel
GC2016-01
Bruce Keafer
Woods Hole Oceanographic Institution
Cruise: SI2017-01
SI2017-01
M/V Scarlett Isabella
vessel
SI2017-01
Bruce Keafer
Woods Hole Oceanographic Institution
Cruise: WJ2017-01
WJ2017-01
R/V Warren Jr.
vessel
WJ2017-01
Bruce Keafer
Woods Hole Oceanographic Institution
Cruise: TI603
TI603
R/V Tioga
Community Standard Description
International Council for the Exploration of the Sea
R/V Tioga
vessel
TI603
Bruce Keafer
Woods Hole Oceanographic Institution
Cruise: TI606
TI606
R/V Tioga
Community Standard Description
International Council for the Exploration of the Sea
R/V Tioga
vessel
TI606
Bruce Keafer
Woods Hole Oceanographic Institution
Cruise: TI667
TI667
R/V Tioga
Community Standard Description
International Council for the Exploration of the Sea
R/V Tioga
vessel
TI667
Bruce Keafer
Woods Hole Oceanographic Institution
Cruise: TI670
TI670
R/V Tioga
Community Standard Description
International Council for the Exploration of the Sea
R/V Tioga
vessel
TI670
Bruce Keafer
Woods Hole Oceanographic Institution
Cruise: TI672
TI672
R/V Tioga
Community Standard Description
International Council for the Exploration of the Sea
R/V Tioga
vessel
TI672
Bruce Keafer
Woods Hole Oceanographic Institution
Cruise: TI691
TI691
R/V Tioga
Community Standard Description
International Council for the Exploration of the Sea
R/V Tioga
vessel
TI691
Bruce Keafer
Woods Hole Oceanographic Institution
Cruise: TI747
TI747
R/V Tioga
Community Standard Description
International Council for the Exploration of the Sea
R/V Tioga
vessel
TI747
Bruce Keafer
Woods Hole Oceanographic Institution
Cruise: TI751
TI751
R/V Tioga
Community Standard Description
International Council for the Exploration of the Sea
R/V Tioga
vessel
TI751
Bruce Keafer
Woods Hole Oceanographic Institution
Cruise: TI758
TI758
R/V Tioga
Community Standard Description
International Council for the Exploration of the Sea
R/V Tioga
vessel
TI758
Bruce Keafer
Woods Hole Oceanographic Institution
Cruise: TI813
TI813
R/V Tioga
Community Standard Description
International Council for the Exploration of the Sea
R/V Tioga
vessel
TI813
Bruce Keafer
Woods Hole Oceanographic Institution
Cruise: TI817
TI817
R/V Tioga
Community Standard Description
International Council for the Exploration of the Sea
R/V Tioga
vessel
TI817
Bruce Keafer
Woods Hole Oceanographic Institution
Cruise: TI831
TI831
R/V Tioga
Community Standard Description
International Council for the Exploration of the Sea
R/V Tioga
vessel
TI831
Katherine Hubbard
Woods Hole Oceanographic Institution
Cruise: TI906
TI906
R/V Tioga
Community Standard Description
International Council for the Exploration of the Sea
R/V Tioga
vessel
TI906
Bruce Keafer
Woods Hole Oceanographic Institution
Cruise: TI972
TI972
R/V Tioga
Community Standard Description
International Council for the Exploration of the Sea
R/V Tioga
vessel
TI972
Bruce Keafer
Woods Hole Oceanographic Institution
Cruise: TI978
TI978
R/V Tioga
Community Standard Description
International Council for the Exploration of the Sea
R/V Tioga
vessel
TI978
Bruce Keafer
Woods Hole Oceanographic Institution
R/V Connecticut
R/V Connecticut
vessel
R/V Gulf Challenger
Community Standard Description
International Council for the Exploration of the Sea
R/V Gulf Challenger
vessel
M/V Scarlett Isabella
vessel
R/V Tioga
Community Standard Description
International Council for the Exploration of the Sea
R/V Tioga
vessel