http://lod.bco-dmo.org/id/dataset/835968
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2021-01-08
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Photobehavior for octopus (‘Octopus bimaculatus’) and squid (‘Doryteuthis opalescens’) paralarvae during exposure to 9 light irradiance levels and 4 oxygen conditions in trials conducted in April of 2019
2021-08-30
publication
2021-08-30
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2021-03-23
publication
https://doi.org/10.26008/1912/bco-dmo.835968.1
Lisa A. Levin
University of California-San Diego
principalInvestigator
Nicholas Oesch
University of California-San Diego
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: McCormick, L., Levin, L., Oesch, N. (2021) Photobehavior for octopus (‘Octopus bimaculatus’) and squid (‘Doryteuthis opalescens’) paralarvae during exposure to 9 light irradiance levels and 4 oxygen conditions in trials conducted in April of 2019. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-03-16 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.835968.1 [access date]
Methods and Sampling: <p>This dataset gives the results of photobehavior experiments under different oxygen conditions in larvae of the market squid (Doryteuthis opalescens) and two-spot octopus (Octopus bimaculatus). Photobehavior experiments were conducted to determine whether the impairment of visual physiology observed in marine invertebrate larvae (McCormick et al., 2019) is subsequently affecting visual behavior in marine larvae.</p>
<p>Briefly, animals were placed in a 20cmx4cmx5cm clear plexiglass chamber filled with seawater to 16cm inside a light-tight box with an overhead light stimulus. After animals were placed in the chamber, the oxygen and temperature were measured, and then a 15-minute dark adaptation period was started. The experiment consisted of a series of a 10-s light stimulus followed by 3-minute dark period; this was repeated at 9 different irradiance levels (1= lowest, 9= highest; 0.062-2.45 mol photons/m^2/s) and at 4 different oxygen levels. Oxygen levels were selected based on the 3 visual metrics that were calculated to quantify the effects of reduced pO2 on retinal function (McCormick et al., 2019). V90, V50, and V10 were calculated for each individual larva as the oxygen where there was 90%, 50%, and 10% retinal function remaining, in respect to retinal responses in normoxia (surface-ocean oxygen levels). For squid, oxygen levels were 1) Normoxia (21.1 kPa); 2) V50 (12.9 kPa); 3) V10 (6.6 kPa); and 4) Low (4.2 kPa). For octopus, oxygen levels were 1) Normoxia (21.3 kPa); 2) V90 (10.7 kPa); 3) V50 (7.4 kPa); and 4) V10 (5.8 kPa). All experiments were video recorded and analyzed using Noldus EthoVision XT software (version 15).<br />
<br />
Oxygen and temperature were measured using a Microx4 (PreSens) oxygen meter and a Pst-7 oxygen optode probe.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1829623 Award URL: http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1829623
completed
Lisa A. Levin
University of California-San Diego
858-534-3579
9500 Gilman Drive Mail Code: 0218
La Jolla
CA
92093-0218
USA
llevin@ucsd.edu
pointOfContact
Nicholas Oesch
University of California-San Diego
858-534-2991
9500 Gilman Dr.
La Jolla
CA
92093-0109
USA
noesch@mail.ucsd.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Trial
O2
Time_Bin
Y_All1
Y_All2
Y_All3
Y_All4
Y_All5
Y_All6
Y_All7
Y_All8
Y_All9
Y_All10
Y_All11
Y_All12
Y_All13
Y_All14
Y_All15
Y_All16
Y_mean
Stimulus
bin_30s
Irrad
Video Camera
theme
None, User defined
experiment id
treatment
time_elapsed
No BCO-DMO term
irradiance
featureType
BCO-DMO Standard Parameters
Camera
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Vision-mediated influence of low oxygen on the physiology and ecology of marine larvae
https://www.bco-dmo.org/project/775843
Vision-mediated influence of low oxygen on the physiology and ecology of marine larvae
<p>NSF abstract:<br />
Oxygen is being lost in the ocean worldwide as a result of ocean warming and the input of nutrients from land. Vision requires a large amount of oxygen, and may be less effective or require more light when oxygen is in short supply. This is especially true for active marine animals with complex eyes and visual capabilities, including active arthropods (crabs), cephalopods (squid), and fish. The California coastal waters exhibit a sharp drop in oxygen and light with increasing water depth. This project examines how visual physiology and ecology in young (larval) highly visual marine animals respond to oxygen loss, with a focus on key fisheries and aquaculture species. Experiments and observations will test the hypothesis that oxygen stress will change the light required for these organisms to see effectively, influencing the water depths where they can live and survive. The project will provide interdisciplinary experiences to students and an early career scientist and inform both the public (through outreach at the Birch Aquarium at Scripps Institution of Oceanography) and policy makers about the effects of oxygen decline in the ocean.</p>
<p>Negative effects of oxygen loss on vision have been described for humans and other terrestrial organisms, but never in the marine environment, despite the large changes in oxygen that can occur with depth and over time in the ocean, and the high metabolic demand of visual systems. This project will test the effects of low oxygen on vision in 3 combinations of eye design and photo-transduction mechanisms: compound eye with rhabdomeric photoreceptors (arthropods), simple eye with rhabdomeric photoreceptors (cephalopods), and simple eye with ciliary photoreceptors (fish). A series of oxygen- and light-controlled laboratory experiments will be conducted on representative taxa of each group including the tuna crab, Pleuroncodes planipes; the market squid, Doryteuthis opalescens, and the white sea bass, Atractoscion nobilis. In vivo electrophysiology and behavioral phototaxis experiments will identify new oxygen metrics for visual physiology and function, and will be compared to metabolic thresholds determined in respiration experiments. Hydrographic data collected over 3 decades by the CalCOFI program in the Southern California Bight will be evaluated with respect to visual and metabolic limits to determine the consequences of oxygen variation on the critical luminoxyscape (range of oxygen and light conditions required for visual physiology and function in target species) boundary in each species. Findings for the three vision-based functional groups may test whether oxygen-limited visual responses offer an additional explanation for the shoaling of species distributions among highly visual pelagic taxa in low oxygen, and will help to focus future research efforts and better understand the stressors contributing to habitat compression with expanding oxygen loss in the ocean.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
Vision under hypoxia
largerWorkCitation
project
eng; USA
oceans
2019-04-01
2019-04-01
Southern California Bight, Northeast Pacific Ocean
0
BCO-DMO catalogue of parameters from Photobehavior for octopus (‘Octopus bimaculatus’) and squid (‘Doryteuthis opalescens’) paralarvae during exposure to 9 light irradiance levels and 4 oxygen conditions in trials conducted in April of 2019
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/859813.rdf
Name: Trial
Units: unitless
Description: Reference to the specific trial the data are obtained from. Format is SquidX_O2, where X is the trial number and O2 is the oxygen condition (see methodology for more details). “Squid” refers to ‘Doryteuthis opalescens’. “Obimac” refers to ‘Octopus bimaculatus’.
http://lod.bco-dmo.org/id/dataset-parameter/859814.rdf
Name: O2
Units: unitless
Description: Oxygen level for the given trial (see methodology for details)
http://lod.bco-dmo.org/id/dataset-parameter/859815.rdf
Name: Time_Bin
Units: seconds
Description: Time of the experiment in seconds. Data are binned for every second.
http://lod.bco-dmo.org/id/dataset-parameter/859816.rdf
Name: Y_All1
Units: unitless
Description: Vertical position data in mm for larva number 1 of the 16 tracked squid for the entire chamber (or 5-6 tracked octopus larvae, see Trial name). See methodology for explanation of analysis methods. Blank cells indicate the larva was not being tracked during that time. Vertical position is between -80 (bottom of the chamber) to 80 (top of the chamber). 0 is the center of the chamber.
http://lod.bco-dmo.org/id/dataset-parameter/859817.rdf
Name: Y_All2
Units: unitless
Description: Vertical position data in mm for larva number 2 of the 16 tracked squid for the entire chamber (or 5-6 tracked octopus larvae, see Trial name). See methodology for explanation of analysis methods. Blank cells indicate the larva was not being tracked during that time. Vertical position is between -80 (bottom of the chamber) to 80 (top of the chamber). 0 is the center of the chamber.
http://lod.bco-dmo.org/id/dataset-parameter/859818.rdf
Name: Y_All3
Units: unitless
Description: Vertical position data in mm for larva number 3 of the 16 tracked squid for the entire chamber (or 5-6 tracked octopus larvae, see Trial name). See methodology for explanation of analysis methods. Blank cells indicate the larva was not being tracked during that time. Vertical position is between -80 (bottom of the chamber) to 80 (top of the chamber). 0 is the center of the chamber.
http://lod.bco-dmo.org/id/dataset-parameter/859819.rdf
Name: Y_All4
Units: unitless
Description: Vertical position data in mm for larva number 4 of the 16 tracked squid for the entire chamber (or 5-6 tracked octopus larvae, see Trial name). See methodology for explanation of analysis methods. Blank cells indicate the larva was not being tracked during that time. Vertical position is between -80 (bottom of the chamber) to 80 (top of the chamber). 0 is the center of the chamber.
http://lod.bco-dmo.org/id/dataset-parameter/859820.rdf
Name: Y_All5
Units: unitless
Description: Vertical position data in mm for larva number 5 of the 16 tracked squid for the entire chamber (or 5-6 tracked octopus larvae, see Trial name). See methodology for explanation of analysis methods. Blank cells indicate the larva was not being tracked during that time. Vertical position is between -80 (bottom of the chamber) to 80 (top of the chamber). 0 is the center of the chamber.
http://lod.bco-dmo.org/id/dataset-parameter/859821.rdf
Name: Y_All6
Units: unitless
Description: Vertical position data in mm for larva number 6 of the 16 tracked squid for the entire chamber (or 5-6 tracked octopus larvae, see Trial name). See methodology for explanation of analysis methods. Blank cells indicate the larva was not being tracked during that time. Vertical position is between -80 (bottom of the chamber) to 80 (top of the chamber). 0 is the center of the chamber.
http://lod.bco-dmo.org/id/dataset-parameter/859822.rdf
Name: Y_All7
Units: unitless
Description: Vertical position data in mm for larva number 7 of the 16 tracked squid for the entire chamber (or 5-6 tracked octopus larvae, see Trial name). See methodology for explanation of analysis methods. Blank cells indicate the larva was not being tracked during that time. Vertical position is between -80 (bottom of the chamber) to 80 (top of the chamber). 0 is the center of the chamber.
http://lod.bco-dmo.org/id/dataset-parameter/859823.rdf
Name: Y_All8
Units: unitless
Description: Vertical position data in mm for larva number 8 of the 16 tracked squid for the entire chamber (or 5-6 tracked octopus larvae, see Trial name). See methodology for explanation of analysis methods. Blank cells indicate the larva was not being tracked during that time. Vertical position is between -80 (bottom of the chamber) to 80 (top of the chamber). 0 is the center of the chamber.
http://lod.bco-dmo.org/id/dataset-parameter/859824.rdf
Name: Y_All9
Units: unitless
Description: Vertical position data in mm for larva number 9 of the 16 tracked squid for the entire chamber (or 5-6 tracked octopus larvae, see Trial name). See methodology for explanation of analysis methods. Blank cells indicate the larva was not being tracked during that time. Vertical position is between -80 (bottom of the chamber) to 80 (top of the chamber). 0 is the center of the chamber.
http://lod.bco-dmo.org/id/dataset-parameter/859825.rdf
Name: Y_All10
Units: unitless
Description: Vertical position data in mm for larva number 10 of the 16 tracked squid for the entire chamber (or 5-6 tracked octopus larvae, see Trial name). See methodology for explanation of analysis methods. Blank cells indicate the larva was not being tracked during that time. Vertical position is between -80 (bottom of the chamber) to 80 (top of the chamber). 0 is the center of the chamber.
http://lod.bco-dmo.org/id/dataset-parameter/859826.rdf
Name: Y_All11
Units: unitless
Description: Vertical position data in mm for larva number 11 of the 16 tracked squid for the entire chamber (or 5-6 tracked octopus larvae, see Trial name). See methodology for explanation of analysis methods. Blank cells indicate the larva was not being tracked during that time. Vertical position is between -80 (bottom of the chamber) to 80 (top of the chamber). 0 is the center of the chamber.
http://lod.bco-dmo.org/id/dataset-parameter/859827.rdf
Name: Y_All12
Units: unitless
Description: Vertical position data in mm for larva number 12 of the 16 tracked squid for the entire chamber (or 5-6 tracked octopus larvae, see Trial name). See methodology for explanation of analysis methods. Blank cells indicate the larva was not being tracked during that time. Vertical position is between -80 (bottom of the chamber) to 80 (top of the chamber). 0 is the center of the chamber.
http://lod.bco-dmo.org/id/dataset-parameter/859828.rdf
Name: Y_All13
Units: unitless
Description: Vertical position data in mm for larva number 13 of the 16 tracked squid for the entire chamber (or 5-6 tracked octopus larvae, see Trial name). See methodology for explanation of analysis methods. Blank cells indicate the larva was not being tracked during that time. Vertical position is between -80 (bottom of the chamber) to 80 (top of the chamber). 0 is the center of the chamber.
http://lod.bco-dmo.org/id/dataset-parameter/859829.rdf
Name: Y_All14
Units: unitless
Description: Vertical position data in mm for larva number 14 of the 16 tracked squid for the entire chamber (or 5-6 tracked octopus larvae, see Trial name). See methodology for explanation of analysis methods. Blank cells indicate the larva was not being tracked during that time. Vertical position is between -80 (bottom of the chamber) to 80 (top of the chamber). 0 is the center of the chamber.
http://lod.bco-dmo.org/id/dataset-parameter/859830.rdf
Name: Y_All15
Units: unitless
Description: Vertical position data in mm for larva number 15 of the 16 tracked squid for the entire chamber (or 5-6 tracked octopus larvae, see Trial name). See methodology for explanation of analysis methods. Blank cells indicate the larva was not being tracked during that time. Vertical position is between -80 (bottom of the chamber) to 80 (top of the chamber). 0 is the center of the chamber.
http://lod.bco-dmo.org/id/dataset-parameter/859831.rdf
Name: Y_All16
Units: unitless
Description: Vertical position data in mm for larva number 16 of the 16 tracked squid for the entire chamber (or 5-6 tracked octopus larvae, see Trial name). See methodology for explanation of analysis methods. Blank cells indicate the larva was not being tracked during that time. Vertical position is between -80 (bottom of the chamber) to 80 (top of the chamber). 0 is the center of the chamber.
http://lod.bco-dmo.org/id/dataset-parameter/859832.rdf
Name: Y_mean
Units: unitless
Description: Mean vertical position for all individuals in the trial for each given time bin. Chamber positions are the same as above (range -80 to 80).
http://lod.bco-dmo.org/id/dataset-parameter/859833.rdf
Name: Stimulus
Units: unitless
Description: The experimental light stimulus the data are from. The stimulus will either be Light for during a light stimulus (1-9), or Dark for the dark period (no light) immediately after the light stimulus (1-9). DkCtrl1 and DkCtrl2 refer to the 2-3 minute periods of darkness before and after each experiment that serve as a control for normal swimming behavior.
http://lod.bco-dmo.org/id/dataset-parameter/859834.rdf
Name: bin_30s
Units: unitless
Description: The 30 second time bin of analysis before the termination of the light stimulus and 30 seconds (s) after the termination of the light stimulus. The 30 s before the light stimulus refers to 20 s in the darkness + the 10 s light stimulus. Values are similar to irradiance levels (e.g. 9.1 is the 30 s before the termination of Light9 and 8.9 is the 30 s after the termination of Light9 (see "Stimulus" description)
http://lod.bco-dmo.org/id/dataset-parameter/859835.rdf
Name: Irrad
Units: mol photons/m^2/s
Description: Irradiance of the 9 experimental light stimuli. 0 refers to darkness.
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
10479784
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/ab95bde2-b5a6-578e-ba5b-bd4d1b2a7274/content
download
https://doi.org/10.26008/1912/bco-dmo.835968.1
download
onLine
dataset
<p>This dataset gives the results of photobehavior experiments under different oxygen conditions in larvae of the market squid (Doryteuthis opalescens) and two-spot octopus (Octopus bimaculatus). Photobehavior experiments were conducted to determine whether the impairment of visual physiology observed in marine invertebrate larvae (McCormick et al., 2019) is subsequently affecting visual behavior in marine larvae.</p>
<p>Briefly, animals were placed in a 20cmx4cmx5cm clear plexiglass chamber filled with seawater to 16cm inside a light-tight box with an overhead light stimulus. After animals were placed in the chamber, the oxygen and temperature were measured, and then a 15-minute dark adaptation period was started. The experiment consisted of a series of a 10-s light stimulus followed by 3-minute dark period; this was repeated at 9 different irradiance levels (1= lowest, 9= highest; 0.062-2.45 mol photons/m^2/s) and at 4 different oxygen levels. Oxygen levels were selected based on the 3 visual metrics that were calculated to quantify the effects of reduced pO2 on retinal function (McCormick et al., 2019). V90, V50, and V10 were calculated for each individual larva as the oxygen where there was 90%, 50%, and 10% retinal function remaining, in respect to retinal responses in normoxia (surface-ocean oxygen levels). For squid, oxygen levels were 1) Normoxia (21.1 kPa); 2) V50 (12.9 kPa); 3) V10 (6.6 kPa); and 4) Low (4.2 kPa). For octopus, oxygen levels were 1) Normoxia (21.3 kPa); 2) V90 (10.7 kPa); 3) V50 (7.4 kPa); and 4) V10 (5.8 kPa). All experiments were video recorded and analyzed using Noldus EthoVision XT software (version 15).<br />
<br />
Oxygen and temperature were measured using a Microx4 (PreSens) oxygen meter and a Pst-7 oxygen optode probe.</p>
Specified by the Principal Investigator(s)
<p>All experiments were video recorded using Bonsai and later analyzed using Noldus EthoVision XT software (version 15). Analysis of videos allowed for 16 individuals to be tracked at once. Vertical position data is in mm from +80mm which is the surface of the water (closest to the light stimulus) and -80 which is the bottom of the chamber; 0 indicates the center of the chamber. Oxygen data was analyzed using PreSens Measurement Studio 2. Post-processing analysis was completed in R Studio (version 3.3.3).<br />
<br />
BCO-DMO Data Manager Processing Notes (v1, 2021-08-30):<br />
* Data from files&nbsp;VisualBehavior_Dopalescens_Vision3.txt and&nbsp;VisualBehavior_Obimaculatus_Vision3.txt imported in the BCO-DMO data system and combined into one data table.&nbsp;<br />
*&nbsp;Data files imported with missing data identifier "NA" .&nbsp; The missing data value served from this page will depend upon the data format (e.g. Matlab files will have missing data values as NaN).<br />
* column 30s_bin changed to "bin_30s" to conform with BCO-DMO naming convention (can't start with a number)<br />
* Files were originally submitted and archived as version 1 on&nbsp;2021-03-16 but were later revised and resubmitted to BCO-DMO while the dataset was still embargoed.&nbsp; The final version 1 is version date is 2021-08-30 for the archvied version of this dataset (DOI:&nbsp;10.26008/1912/bco-dmo.835968.1).</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Video Camera
Video Camera
PI Supplied Instrument Name: Video Camera Instrument Name: Camera Instrument Short Name:camera Instrument Description: All types of photographic equipment including stills, video, film and digital systems. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/311/