<div><p>We conducted short term (6-h) NO3- uptake experiments in deckboard incubators. Each incubator was uniformly shaded using clear or blue-tinted acrylic sheets to achieve three light levels as determined by simultaneously using a 2-π LI-COR photosynthetically active radiation (PAR) sensor to measure downwelling irradiance and a 4-π water-proof LI-COR PAR sensor to measure ambient irradiance within the incubator. These calibrations were done on the ship to account for light reflection or ship shading. Incubation light levels for the NF17 cruise were determined to be 145% (clear, surface), 79% (mixed layer), and 21% (lower mixed layer) of surface irradiance. For the NF1802 cruise, the clear incubator was replaced with one screened to 1.7% surface irradiance to mimic deep chlorophyll maximum light. All incubators were cooled with mixed-layer seawater. Samples were drawn from depths near these light levels (as determined from noon CTD casts with CTD-mounted PAR sensor). <br />
To determine patterns of nitrate uptake with depth, three 2.7-L samples from each of the three incubator light levels (e.g. 5 m, 15 m, 45 m for surface, 79% and 21% light levels, respectively) were collected near dusk and placed in the incubators until dawn (ship schedule and CTD water budget did not allow dawn sampling for these experiments). At dawn triplicate samples from each depth were spiked with 15NO3- (final concentration of 10 or 8 nmol L-1 on NF17 and NF18, respectively) and incubated for 6-h (dawn to roughly local noon). At the end of the incubation bottles were immediately vacuum filtered onto pre-combusted 25-mm GF/F filters in the dark. Filters were rinsed with filtered seawater, wrapped in foil and stored at 80°C. On land, samples were fumigated with HCl vapor to remove inorganic carbon, dried, and placed inside a tin cup for C/N and isotopic analysis at the UC Davis stable isotope facility. NO3- uptake rates in each incubation bottle (and associated uncertainties) were determined using equations in Stukel (2020).</p></div>
<div><p>In addition to the funding sources listed in the "Funding Source" section, this dataset was partially funded by:</p>
<p>National Oceanic and Atmospheric Administration's RESTORE Program Grant (Project Title: Effects of nitrogen sources and plankton food-web dynamics on habitat quality for the larvae of Atlantic bluefin tuna in the Gulf of Mexico) under federal funding opportunity NOAA-NOS-NCCOS-2017-2004875, including NOAA JIMAR Cooperative Agreement, award #NA16NMF4320058, NOAA CIMAS Cooperative Agreement, award #NA15OAR4320064, and NOAA CIMEAS Cooperative Agreement, award #NA15OAR4320071.</p></div>
Gulf of Mexico deckboard nitrate uptake
<div><p>In situ nitrate uptake rates were estimated using either equation ρkan,is (Eq. 6) or ρreg,is (Eq. 14) from Stukel (2020). ρkan,is was used if there was not evidence for high recycling within the incubation bottle (Φ<0.6). ρreg,is was used if there was evidence for high recycling within the incubation bottle (Φ>0.6). See Fig. 4 of Stukel (2020). </p>
<p>BCO-DMO Data Manager Processing Notes:<br />
* Data in original excel file "Deckboard Nitrate Uptake.xlsx" exported as csv with the formatting that was set in Excel.<br />
* Converted Date to ISO 8601 format yyyy-mm-dd<br />
* modified parameter names to conform with BCO-DMO naming conventions: only A-Za-z0-9 and underscore allowed. Can not start with a number. (spaces, +, and - changed to underscores). <br />
* Removed percent character "%" from the values in the Light_Level column so it could be typed as numeric. The units (percent) are described in the parameter description section.</p></div>
836181
Gulf of Mexico deckboard nitrate uptake
2021-01-12T15:36:02-05:00
2021-01-12T15:36:02-05:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:836181
Nitrate uptake from short-term 6-hour deckboard incubations on R/V Nancy Foster cruises NF1704 and NF1802 in the Gulf of Mexico in May of 2017 and 2018
Nitrate uptake from short-term 6-hour deckboard incubations on R/V Nancy Foster cruises NF1704 and NF1802 in the Gulf of Mexico in May of 2017 and 2018.
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Stukel, M., Kelly, T. (2021) Nitrate uptake from short-term 6-hour deckboard incubations on R/V Nancy Foster cruises NF1704 and NF1802 in the Gulf of Mexico in May of 2017 and 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-01-12 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.836181.1 [access date]
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10.26008/1912/bco-dmo.836181.1
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2021-01-12
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