http://lod.bco-dmo.org/id/dataset/839645
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2021-02-03
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Biogeochemical data on cryptic methane cycling in hypersaline sediments of the Carpinteria Salt Marsh Reserve, California
2021-02-04
publication
2021-02-04
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2021-02-11
publication
https://doi.org/10.26008/1912/bco-dmo.839645.1
Tina Treude
University of California-Los Angeles
principalInvestigator
Sebastian Krause
University of California-Los Angeles
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Treude, T., Krause, S. (2021) Biogeochemical data on cryptic methane cycling in hypersaline sediments of the Carpinteria Salt Marsh Reserve, California. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-02-04 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.839645.1 [access date]
Carpinteria Salt Marsh Reserve Biogeochemistry Dataset Description: Methods and Sampling: <p>Sediments were collected from the hypersaline pool within the Carpinteria Salt Marsh Reserve using large (20 cm i.d.) and small (2.6 cm i.d.) pushcores. Porewater was separated from sediment by centrifugation (4300 x g for 20 mins) and subsampled for further analysis described below.</p>
<p>Porewater sulfate and chloride concentrations were determined by ion chromatography (Metrohm 761). Porewater salinity was calculated from porewater chloride concentrations, using Knudson’s equation (Knudsen, 1901). Porewater sulfide and iron (II) concentrations were determined spectrophotometrically (Shimadzu UV-Spectrophotometer UV-1800) according to Cline, (1969) and Grasshoff et al., (1999) respectively. Porewater methane concentrations were determined by gas chromatography (Shimadzu GC-2014). Sediment porosity was determined by drying sediments at 75°C for five days and calculated using the difference between wet and dry weights, divided by the volume of sediment.</p>
<p>Sulfate reduction rates were determined by injecting carrier-free 35S-Sulfate into intact whole round cores according to Jørgensen (1978); and incubated for 1 day. Sediment samples were then analyzed according to the cold-chromium distillation (Kallmeyer et al., 2004).</p>
<p>AOM rates were determined by injecting 14C-Methane dissolved in anoxic Milliq into intact whole round cores similar to sulfate reduction rate determinations; and incubated for 1 day. Sediment samples were then analyzed according to Treude et al. (2005) and Joye et al. (2004).</p>
Funding provided by NSF Division of Earth Sciences (NSF EAR) Award Number: EAR-1852912 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1852912
completed
Tina Treude
University of California-Los Angeles
310-267-5213
Department of Earth, Planetary, and Space Sciences 595 Charles E. Young Drive, East
Los Angeles
CA
90095-1567
USA
ttreude@g.ucla.edu
pointOfContact
Sebastian Krause
University of California-Los Angeles
Department of Earth, Planetary, and Space Sciences 595 Charles E. Young Drive, East
Los Angeles
CA
90095-1567
USA
sjkrause@ucsb.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Date
Time_Local_PST
Time_UTC
Latitude
Longitude
Pushcore_Inner_Diameter
Sediment_Depth
Porewater_Sulfate
Salinity
Porewater_Iron_II
Porewater_Sulfide
Porosity
Sulfate_Reduction
Methane
AOM
ISO_DateTime_UTC
centrifugation
Shimadzu GC-2014
ion chromatography
Polycarbonate push cores large (20 cm I.d.) and small (2.6 cm I.d.)
Shimadzu UV-Spectrophotometer UV-1800
theme
None, User defined
date
time of day
latitude
longitude
diameter
depth below seafloor
sulfate
salinity
Iron
sulfide
sediment porosity
methane
ISO_DateTime_UTC
featureType
BCO-DMO Standard Parameters
Centrifuge
Gas Chromatograph
Ion Chromatograph
Push Corer
UV Spectrophotometer-Shimadzu
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Deciphering the Cryptic Cycling of Methane in Sediments of a Coastal Wetland
https://osprey.bco-dmo.org/project/810674
Deciphering the Cryptic Cycling of Methane in Sediments of a Coastal Wetland
<p><em>NSF Award Abstract:</em><br />
This research project investigates the close relationship between methane production and consumption in sediments by microbes in the oxygen-free zone of a coastal wetland in Southern California. The direct exchange of methane between those two closely related microbial groups, which has been named "cryptic methane cycling", has only recently been identified and little is known about its importance in reducing methane emissions from coastal wetlands. This research will reveal how carbon moves between the two types of microbes, and will identify the microbes involved. It will also reveal the important metabolic reactions responsible and the balance between methane production and consumption under different environmental conditions. Towards broader impacts, this project will provide training for two undergraduate and one graduate student in interdisciplinary wetland science and state of the art laboratory methods. A new freshmen course on global methane emissions will bring undergraduate students into the field to provide education on local coastal wetland environments. Results of the project will be disseminated to public and academic groups and will provide a better understanding of methane production and consumption in coastal wetlands.</p>
<p>Concentrations of atmospheric methane have more than doubled since the pre-industrial era, hence we urgently need to understand the mechanisms that control the emission of this potent greenhouse gas. Recent studies have provided the first evidence for the simultaneous microbial production and consumption of methane in the sulfate reduction zone of organic-rich sediments, a process named the "cryptic methane cycle." In this process, methane is proposed to be passed directly from methylotrophic methanogenesis to anaerobic oxidation of methane (AOM). However, little is known about the identity of the organisms involved, the trail of carbon from one metabolism to the other, or the environmental net result of the two processes. Without the details of this metabolic relationship, methane budgets of sediments remain incomplete. Coastal wetlands are of particular interest for the study of cryptic methane cycling, because their organic and sulfate rich sediments promote the production of methylated substrates for methylotrophic methanogenesis and provide electron acceptors for AOM. Yet, anaerobic microbial removal of methane from this ecosystem, particularly along the sulfate gradient between ocean and land, is still not well understood. Towards intellectual merit, this study elucidates the identity of methanogenic and methanotrophic archaea involved in cryptic methane cycling in a coastal wetland as well as the selection of electron acceptors that fuel methane removal in this metabolic relationship. The research provides new metabolic clues to unravel the versatility of the enzymatic machinery that drives methanogenesis and AOM. By capturing environmental factors that control the balance between the two processes working in close proximity, the results of this work further provide an enhanced understanding of methane dynamics in coastal wetland sediments. This information can be applied to biogeochemical models to improve the prediction of methane emissions from this ecosystem, which is found throughout the global coastal zone. Towards broader impacts, the research provides training in innovative analytical and experimental techniques to two undergraduate and one graduate student. The project further engages 20 undergraduates per year in a newly developed freshman seminar, "Methane - the Other Greenhouse Problem" including a field trip to the local wetland. The goal of the seminar will be to educate students, including those not entering STEM fields, about global sources and sinks of methane and its involvement in global warming now and in the future. Results of this study will be broadly disseminated to educational and public outreach platforms, such as high schools, community colleges, and environmental non-profits to teach scientific methodologies, concepts of biogeochemical cycling, and enhance the appreciation of this vital coastal environment.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
Cryptic CH4 Cycling
largerWorkCitation
project
eng; USA
oceans
-119.536
-119.536
34.3989
34.3989
2018-06-18
2018-06-18
Carpinteria Salt Marsh Reserve, California, USA
0
BCO-DMO catalogue of parameters from Biogeochemical data on cryptic methane cycling in hypersaline sediments of the Carpinteria Salt Marsh Reserve, California
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/839670.rdf
Name: Date
Units: unitless
Description: <p>Date of sample collection; format: YYYY-MM-DD</p>
http://lod.bco-dmo.org/id/dataset-parameter/839671.rdf
Name: Time_Local_PST
Units: unitless
Description: <p>Time of sample collection (local time zone, PST); format: hh:mm:ss</p>
http://lod.bco-dmo.org/id/dataset-parameter/839672.rdf
Name: Time_UTC
Units: unitless
Description: <p>Time of sample collection (UTC); format: hh:mm:ss</p>
http://lod.bco-dmo.org/id/dataset-parameter/839673.rdf
Name: Latitude
Units: degrees North
Description: <p>Latitude of sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/839674.rdf
Name: Longitude
Units: degrees East
Description: <p>Longitude of sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/839675.rdf
Name: Pushcore_Inner_Diameter
Units: centimeters (cm)
Description: <p>Inner diameter of pushcore</p>
http://lod.bco-dmo.org/id/dataset-parameter/839676.rdf
Name: Sediment_Depth
Units: centimeters (cm)
Description: <p>Sediment depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/839677.rdf
Name: Porewater_Sulfate
Units: millimolar (mM)
Description: <p>Porewater Sulfate Concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/839678.rdf
Name: Salinity
Units: PSU
Description: <p>Porewater Salinity Concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/839679.rdf
Name: Porewater_Iron_II
Units: micromolar (uM)
Description: <p>Porewater Iron (II) Concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/839680.rdf
Name: Porewater_Sulfide
Units: micromolar (uM)
Description: <p>Porewater Sulfide Concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/839681.rdf
Name: Porosity
Units: unitless
Description: <p>Sediment Porosity (v/v)</p>
http://lod.bco-dmo.org/id/dataset-parameter/839682.rdf
Name: Sulfate_Reduction
Units: nanomoles per cubic centimeter per day (nmol cm-3 d-1)
Description: <p>Microbial Sulfate Reduction Rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/839683.rdf
Name: Methane
Units: micromolar (uM)
Description: <p>Porewater Methane Concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/839684.rdf
Name: AOM
Units: nanomoles per cubic centimeter per day (nmol cm-3 d-1)
Description: <p>Microbial Anaerobic Oxidation of Methane (AOM) rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/839685.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>Date and time of sample collection (UTC) formatted to ISO8601 standard: YYYY-MM-DDThh:mm:ssZ</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
11070
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/ec9bf7ee-2c09-5b68-a86b-a9a2fd396ef4/content
download
https://doi.org/10.26008/1912/bco-dmo.839645.1
download
onLine
dataset
<p>Sediments were collected from the hypersaline pool within the Carpinteria Salt Marsh Reserve using large (20 cm i.d.) and small (2.6 cm i.d.) pushcores. Porewater was separated from sediment by centrifugation (4300 x g for 20 mins) and subsampled for further analysis described below.</p>
<p>Porewater sulfate and chloride concentrations were determined by ion chromatography (Metrohm 761). Porewater salinity was calculated from porewater chloride concentrations, using Knudson’s equation (Knudsen, 1901). Porewater sulfide and iron (II) concentrations were determined spectrophotometrically (Shimadzu UV-Spectrophotometer UV-1800) according to Cline, (1969) and Grasshoff et al., (1999) respectively. Porewater methane concentrations were determined by gas chromatography (Shimadzu GC-2014). Sediment porosity was determined by drying sediments at 75°C for five days and calculated using the difference between wet and dry weights, divided by the volume of sediment.</p>
<p>Sulfate reduction rates were determined by injecting carrier-free 35S-Sulfate into intact whole round cores according to Jørgensen (1978); and incubated for 1 day. Sediment samples were then analyzed according to the cold-chromium distillation (Kallmeyer et al., 2004).</p>
<p>AOM rates were determined by injecting 14C-Methane dissolved in anoxic Milliq into intact whole round cores similar to sulfate reduction rate determinations; and incubated for 1 day. Sediment samples were then analyzed according to Treude et al. (2005) and Joye et al. (2004).</p>
Specified by the Principal Investigator(s)
<p><strong>Data Processing:</strong><br />
Excel was used for data processing.</p>
<p><strong>BCO-DMO Processing:</strong><br />
- replaced "NA" with "nd" as missing data identifier;<br />
- added ISO8601 UTC date/time column;<br />
- renamed fields.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
centrifugation
centrifugation
PI Supplied Instrument Name: centrifugation Instrument Name: Centrifuge Instrument Short Name: Instrument Description: A machine with a rapidly rotating container that applies centrifugal force to its contents, typically to separate fluids of different densities (e.g., cream from milk) or liquids from solids.
Shimadzu GC-2014
Shimadzu GC-2014
PI Supplied Instrument Name: Shimadzu GC-2014 Instrument Name: Gas Chromatograph Instrument Short Name:Gas Chromatograph Instrument Description: Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB02/
ion chromatography
ion chromatography
PI Supplied Instrument Name: ion chromatography Instrument Name: Ion Chromatograph Instrument Short Name:Ion Chromatograph Instrument Description: Ion chromatography is a form of liquid chromatography that measures concentrations of ionic species by separating them based on their interaction with a resin. Ionic species separate differently depending on species type and size. Ion chromatographs are able to measure concentrations of major anions, such as fluoride, chloride, nitrate, nitrite, and sulfate, as well as major cations such as lithium, sodium, ammonium, potassium, calcium, and magnesium in the parts-per-billion (ppb) range. (from http://serc.carleton.edu/microbelife/research_methods/biogeochemical/ic.html)
Polycarbonate push cores large (20 cm I.d.) and small (2.6 cm I.d.)
Polycarbonate push cores large (20 cm I.d.) and small (2.6 cm I.d.)
PI Supplied Instrument Name: Polycarbonate push cores large (20 cm I.d.) and small (2.6 cm I.d.) Instrument Name: Push Corer Instrument Short Name: Instrument Description: Capable of being performed in numerous environments, push coring is just as it sounds. Push coring is simply pushing the core barrel (often an aluminum or polycarbonate tube) into the sediment by hand. A push core is useful in that it causes very little disturbance to the more delicate upper layers of a sub-aqueous sediment.
Description obtained from: http://web.whoi.edu/coastal-group/about/how-we-work/field-methods/coring/
Shimadzu UV-Spectrophotometer UV-1800
Shimadzu UV-Spectrophotometer UV-1800
PI Supplied Instrument Name: Shimadzu UV-Spectrophotometer UV-1800 Instrument Name: UV Spectrophotometer-Shimadzu Instrument Short Name:UV Spectrophotometer-Shimadzu Instrument Description: The Shimadzu UV Spectrophotometer is manufactured by Shimadzu Scientific Instruments (ssi.shimadzu.com). Shimadzu manufacturers several models of spectrophotometer; refer to dataset for make/model information. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/