http://lod.bco-dmo.org/id/dataset/839920
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2021-02-05
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Experimental coral physiological and δ15N isotopic measurements in October 2012 at Reef Systems Coral Farm, Ohio.
2022-03-21
publication
2022-03-21
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2022-03-21
publication
https://doi.org/10.26008/1912/bco-dmo.839920.1
Andréa G. Grottoli
Ohio State University
principalInvestigator
Kerri Dobson
Ohio State University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Grottoli, A. G., Dobson, K. (2022) Experimental coral physiological and δ15N isotopic measurements in October 2012 at Reef Systems Coral Farm, Ohio. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-03-21 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.839920.1 [access date]
Coral physiological and δ15N isotopic measurements Dataset Description: <p>Details of instruments used during the experiment can be found in Hoadley et al. (2016).</p>
<p>Other comments on the data:</p>
<ul>
<li>No control data (treatment 1) for <em>A. millepora</em>.</li>
<li>No DOC or POC samples were collected for <em>A. millepora</em>.</li>
<li>No δ<sup>15</sup>N coral host data was collected for <em>T. reniformis</em>, and no δ<sup>15</sup>N whole data was collected for <em>A. millepora</em>.</li>
</ul> Methods and Sampling: <p>Prior to the start of this experiment in September 2012, six colonies of <em>A. millepora</em> and <em>T. reniformis</em> originally collected from 3-10m depth in Fiji (17°29’19”S, 177°23’39”E) were maintained for 18 months at 26.4°C ±0.04 SE in 3785 L recirculating indoor aquaria with artificial seawater (Instant Ocean Reef Crystals) in a greenhouse (700–1000 µmol quanta<sup>-1</sup> m<sup>-2</sup> s<sup>-1</sup>) at the Reef Systems Coral Farm mariculture facility in New Albany, Ohio (40°07'24"N, 82°46'55”W). In January 2012, <em>A. millepora</em> and <em>T. reniformis</em> colonies were divided into coral ramets (n=8 per colony), mounted on 5cm PVC tiles using EcoTech coral glue, and allowed to recover. On 6 August 2012, coral ramets were placed into indoor experimental tanks (57 L) with artificial light (Tek Light T5 actinic lights, 275 μmol quanta<sup>-1</sup> m<sup>-2 </sup>s<sup>-1</sup>, 10:14 hours light:dark diurnal cycle) under ambient conditions (26.4°C, 402 μatm) and allowed to acclimate for four weeks.</p>
<p>The experimental systems in this study were previously outlined in Hoadley <em>et al.</em> (2016). Briefly, from 7 – 16 September 2012, treatment conditions were initiated: temperature, <em>p</em>CO<sub>2</sub>, and nutrients were gradually increased over the course of a week until target conditions in each treatment were reached to minimize shocking any of the corals. The treatments consisted of a control (26.4°C, 402 μatm), elevated <em>p</em>CO<sub>2</sub> (26.4°C, 760 µatm), elevated temperature (29.8°C, 402 μatm), and combined elevated temperature and <em>p</em>CO<sub>2</sub> (29.8°C, 760 μatm). Half of the tanks in each treatment were under ambient nutrient concentrations (0.41 μmol L<sup>-1</sup> NO<sub>3</sub><sup>-</sup> and 0.25 μmol L<sup>-1</sup> PO<sub>4</sub><sup>-3</sup>) and the other half under moderate nutrient concentrations (3.56 μmol L<sup>-1</sup> NO<sub>3</sub><sup>- </sup>and 0.31 μmol L<sup>-1</sup> PO<sub>4</sub><sup>-3</sup>). Experimental conditions (ramping plus target conditions) lasted for 30 days, and coral were fed fresh, two-day old <em>Artemia nauplii</em> (Carolina Biological Supply) twice each week.</p>
Funding provided by NSF Emerging Frontiers Division (NSF EF) Award Number: EF-1041124 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1041124
completed
Andréa G. Grottoli
Ohio State University
614-292-5782
School of Earth Sciences 125 South Oval Mall
Columbus
OH
43210
United States
grottoli.1@osu.edu
pointOfContact
Kerri Dobson
Ohio State University
+1-614-620-9772
School of Earth Sciences 125 South Oval Mall
Columbus
OH
43210
USA
K.L.Dobson@soton.ac.uk
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Date_Collected
Latitude
Longitude
Species
Sample_ID
Treatment
Temperature_Water
pCO2
Nutrient_Concentration
Genotype
Total_Calcification
Gross_Photosynthesis
CZAR
CHARTOC
CTAR
Tissue_Biomass
Chlorophyll_a
Protein
Carbohydrates
Total_lipids
Delta15N_Whole
Delta15N_Algal_Endosymbionts
Delta15N_Coral_Host
Costech elemental analyzer
Elementar Vario EL Cube/Micro Cube elemental analyzer
Thermo Finnigan Delta IV stable isotope ratio mass spectrometer
PDZ Europa 20-20 stable isotope ratio mass spectrometer
Thermo Scientific Genesys
Shimadzu TOC-L total organic carbon analyzer
theme
None, User defined
date
latitude
longitude
species scientific name (binomial)
sample identification
sample description
water temperature at measurement depth
Partial pressure of CO2
calcification
dissolved Oxygen
wet_weight_biomass
chlorophyll a
protein concentration
carbohydrates
total lipids
Nitrogen
featureType
BCO-DMO Standard Parameters
Elemental Analyzer
Elemental Analyzer
Isotope-ratio Mass Spectrometer
Isotope-ratio Mass Spectrometer
Spectrophotometer
Total Organic Carbon Analyzer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)
https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=503477
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)
NSF Climate Research Investment (CRI) activities that were initiated in 2010 are now included under Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES). SEES is a portfolio of activities that highlights NSF's unique role in helping society address the challenge(s) of achieving sustainability. Detailed information about the SEES program is available from NSF (https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=504707).
In recognition of the need for basic research concerning the nature, extent and impact of ocean acidification on oceanic environments in the past, present and future, the goal of the SEES: OA program is to understand (a) the chemistry and physical chemistry of ocean acidification; (b) how ocean acidification interacts with processes at the organismal level; and (c) how the earth system history informs our understanding of the effects of ocean acidification on the present day and future ocean.
Solicitations issued under this program:
NSF 10-530, FY 2010-FY2011
NSF 12-500, FY 2012
NSF 12-600, FY 2013
NSF 13-586, FY 2014
NSF 13-586 was the final solicitation that will be released for this program.
PI Meetings:
1st U.S. Ocean Acidification PI Meeting(March 22-24, 2011, Woods Hole, MA)
2nd U.S. Ocean Acidification PI Meeting(Sept. 18-20, 2013, Washington, DC)
3rd U.S. Ocean Acidification PI Meeting (June 9-11, 2015, Woods Hole, MA – Tentative)
NSF media releases for the Ocean Acidification Program:
Press Release 10-186 NSF Awards Grants to Study Effects of Ocean Acidification
Discovery Blue Mussels "Hang On" Along Rocky Shores: For How Long?
Discovery nsf.gov - National Science Foundation (NSF) Discoveries - Trouble in Paradise: Ocean Acidification This Way Comes - US National Science Foundation (NSF)
Press Release 12-179 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: Finding New Answers Through National Science Foundation Research Grants - US National Science Foundation (NSF)
Press Release 13-102 World Oceans Month Brings Mixed News for Oysters
Press Release 13-108 nsf.gov - National Science Foundation (NSF) News - Natural Underwater Springs Show How Coral Reefs Respond to Ocean Acidification - US National Science Foundation (NSF)
Press Release 13-148 Ocean acidification: Making new discoveries through National Science Foundation research grants
Press Release 13-148 - Video nsf.gov - News - Video - NSF Ocean Sciences Division Director David Conover answers questions about ocean acidification. - US National Science Foundation (NSF)
Press Release 14-010 nsf.gov - National Science Foundation (NSF) News - Palau's coral reefs surprisingly resistant to ocean acidification - US National Science Foundation (NSF)
Press Release 14-116 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: NSF awards $11.4 million in new grants to study effects on marine ecosystems - US National Science Foundation (NSF)
SEES-OA
largerWorkCitation
program
Interactive Effects of Temperature, Nutrients, and Ocean Acidification on Coral Physiology and Calcification
https://osprey.bco-dmo.org/project/528004
Interactive Effects of Temperature, Nutrients, and Ocean Acidification on Coral Physiology and Calcification
<p><em>Extracted from the NSF award abstract:</em> </p>
<p>Atmospheric and sea surface CO2 concentrations are expected to continue to increase substantially over the coming decades resulting in warmer and more acidic oceans, which will greatly stress the health of coral reefs. In addition, ocean margins where most corals live will also see continued increases in human-produced nutrient inputs. While there has recently been a considerable focus on how ocean acidification (due to higher CO2 alone) could negatively impact the growth of reef-building corals due to the projected loss in calcification, the combined impacts of CO2, temperature, and nutrients on coral physiology and calcification are poorly understood. This project will investigate the possible synergistic and antagonistic effects of elevated temperature, CO2, and nutrients on the physiology and internal calcifying chemistry of several species of corals in a laboratory setting. Research tools will include the assessment of coral energy reserves and metabolic demand, symbiotic algal physiology and molecular diversity, coral calcification, and direct measurement of the internal coral pH and carbonate concentration via microprobes. The results from this project have the potential to supply broad scientific impacts regarding how (or if) reef-building corals will survive future climate change scenarios, and will help establish several parameter ranges that could be used to strengthen ocean acidification and coral reef growth models.</p>
OA_coral_physiology
largerWorkCitation
project
eng; USA
oceans
-82.782
-82.782
40.123
40.123
2012-10-06
2012-10-13
Reef Systems Coral Farm, New Albany, Ohio, USA
0
BCO-DMO catalogue of parameters from Experimental coral physiological and δ15N isotopic measurements in October 2012 at Reef Systems Coral Farm, Ohio.
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/839948.rdf
Name: Date_Collected
Units: unitless
Description: <p>Collection date in ISO format (yyyy-mm-dd)</p>
http://lod.bco-dmo.org/id/dataset-parameter/839949.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitude of sampling location, south is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/839950.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitude of sampling location, west is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/839951.rdf
Name: Species
Units: unitless
Description: <p>Taxonomic name of the coral species</p>
http://lod.bco-dmo.org/id/dataset-parameter/839952.rdf
Name: Sample_ID
Units: unitless
Description: <p>ID number of the coral sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/839953.rdf
Name: Treatment
Units: units
Description: <p>No description provided</p>
http://lod.bco-dmo.org/id/dataset-parameter/839954.rdf
Name: Temperature_Water
Units: units
Description: <p>Water temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/839955.rdf
Name: pCO2
Units: micro-atmospheres (µatm)
Description: <p>Partial pressure of CO2</p>
http://lod.bco-dmo.org/id/dataset-parameter/839956.rdf
Name: Nutrient_Concentration
Units: mol/L
Description: <p>Nutrient concentration of the water</p>
http://lod.bco-dmo.org/id/dataset-parameter/839957.rdf
Name: Genotype
Units: unitless
Description: <p>Genotype (parent colony) identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/839958.rdf
Name: Total_Calcification
Units: milligrams per day per square centimeters (mg/day/cm2)
Description: <p>Calcification rate across the entire experiment using buoyant weight, normalized to dry weight, and standardized to the surface area of the coral fragment</p>
http://lod.bco-dmo.org/id/dataset-parameter/839959.rdf
Name: Gross_Photosynthesis
Units: micromoles per minute per square centimeters (µmol/min/cm2)
Description: <p>Gross photosynthesis of the coral fragment in amount of oxygen produced, standardized to time and surface area of the fragment</p>
http://lod.bco-dmo.org/id/dataset-parameter/839960.rdf
Name: CZAR
Units: percentage (%)
Description: <p>Contribution of Zooxanthellae to Animal Respiration</p>
http://lod.bco-dmo.org/id/dataset-parameter/839961.rdf
Name: CHARTOC
Units: percentage (%)
Description: <p>Contribution of Heterotrophy to Animal Respiration from total organic carbon, measured in percentage</p>
http://lod.bco-dmo.org/id/dataset-parameter/839962.rdf
Name: CTAR
Units: percentage (%)
Description: <p>Contribution of Total Acquired fixed carbon relative to animal Respiration, measured in percentage</p>
http://lod.bco-dmo.org/id/dataset-parameter/839963.rdf
Name: Tissue_Biomass
Units: miligrams per square centimeters (mg/cm2)
Description: <p>The ash-free dry weight of the whole tissue (animal host and algal endosymbiont) standardized to surface area</p>
http://lod.bco-dmo.org/id/dataset-parameter/839964.rdf
Name: Chlorophyll_a
Units: micrograms per square centimers (µg/cm2)
Description: <p>The chlorophyll a content of the endosymbiont fraction, standardized to surface area of the fragment</p>
http://lod.bco-dmo.org/id/dataset-parameter/839965.rdf
Name: Protein
Units: Joules per g ash-free dry weight (J/gdw)
Description: <p>The soluble protein content of the animal host fraction, converted into Joules and standardized to ash-free dry weight</p>
http://lod.bco-dmo.org/id/dataset-parameter/839966.rdf
Name: Carbohydrates
Units: Joules per g ash-free dry weight (J/gdw)
Description: <p>The soluble carbohydrate content of the animal host fraction, converted into Joules and standardized to ash-free dry weight</p>
http://lod.bco-dmo.org/id/dataset-parameter/839967.rdf
Name: Total_lipids
Units: Joules per g ash-free dry weight (J/gdw)
Description: <p>The soluble lipid content of the combined animal host and algal endosymbiont fraction, converted to Joules and standardized to ash-free dry weight</p>
http://lod.bco-dmo.org/id/dataset-parameter/839968.rdf
Name: Delta15N_Whole
Units: parts per thousand (‰)
Description: <p>Stable nitrogen isotopes of the combined animal host and algal endosymbiont, relative to air</p>
http://lod.bco-dmo.org/id/dataset-parameter/839969.rdf
Name: Delta15N_Algal_Endosymbionts
Units: parts per thousand (‰)
Description: <p>Stable nitrogen isotopes of the isolated algal endosymbiont, relative to air</p>
http://lod.bco-dmo.org/id/dataset-parameter/839970.rdf
Name: Delta15N_Coral_Host
Units: parts per thousand (‰)
Description: <p>Stable nitrogen isotopes of the isolated animal host, relative to air</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
12991
https://darchive.mblwhoilibrary.org/bitstream/1912/28217/1/dataset-839920_coral-physiological-and-__15n-isotopic-measurements__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.839920.1
download
onLine
dataset
<p>Prior to the start of this experiment in September 2012, six colonies of <em>A. millepora</em> and <em>T. reniformis</em> originally collected from 3-10m depth in Fiji (17°29’19”S, 177°23’39”E) were maintained for 18 months at 26.4°C ±0.04 SE in 3785 L recirculating indoor aquaria with artificial seawater (Instant Ocean Reef Crystals) in a greenhouse (700–1000 µmol quanta<sup>-1</sup> m<sup>-2</sup> s<sup>-1</sup>) at the Reef Systems Coral Farm mariculture facility in New Albany, Ohio (40°07'24"N, 82°46'55”W). In January 2012, <em>A. millepora</em> and <em>T. reniformis</em> colonies were divided into coral ramets (n=8 per colony), mounted on 5cm PVC tiles using EcoTech coral glue, and allowed to recover. On 6 August 2012, coral ramets were placed into indoor experimental tanks (57 L) with artificial light (Tek Light T5 actinic lights, 275 μmol quanta<sup>-1</sup> m<sup>-2 </sup>s<sup>-1</sup>, 10:14 hours light:dark diurnal cycle) under ambient conditions (26.4°C, 402 μatm) and allowed to acclimate for four weeks.</p>
<p>The experimental systems in this study were previously outlined in Hoadley <em>et al.</em> (2016). Briefly, from 7 – 16 September 2012, treatment conditions were initiated: temperature, <em>p</em>CO<sub>2</sub>, and nutrients were gradually increased over the course of a week until target conditions in each treatment were reached to minimize shocking any of the corals. The treatments consisted of a control (26.4°C, 402 μatm), elevated <em>p</em>CO<sub>2</sub> (26.4°C, 760 µatm), elevated temperature (29.8°C, 402 μatm), and combined elevated temperature and <em>p</em>CO<sub>2</sub> (29.8°C, 760 μatm). Half of the tanks in each treatment were under ambient nutrient concentrations (0.41 μmol L<sup>-1</sup> NO<sub>3</sub><sup>-</sup> and 0.25 μmol L<sup>-1</sup> PO<sub>4</sub><sup>-3</sup>) and the other half under moderate nutrient concentrations (3.56 μmol L<sup>-1</sup> NO<sub>3</sub><sup>- </sup>and 0.31 μmol L<sup>-1</sup> PO<sub>4</sub><sup>-3</sup>). Experimental conditions (ramping plus target conditions) lasted for 30 days, and coral were fed fresh, two-day old <em>Artemia nauplii</em> (Carolina Biological Supply) twice each week.</p>
Specified by the Principal Investigator(s)
<p><strong>Calcification.</strong> Net calcification was determined using the buoyant weight technique (Jokiel et al 1978). Each coral fragment was weighed at the beginning and end of the experiment. Buoyant weight was normalized to dry weight.</p>
<p><strong>Gross photosynthesis.</strong> Gross photosynthesis was calculated from net photosynthesis and respiration; these measurements were made on the live coral fragments incubated in sealed chambers (Hoadley et al. 2016).</p>
<p><strong>CZAR, CHARtoc, CTAR.</strong> Gross photosynthesis and respiration were used to calculate CZAR (Muscatine et al. 1981). Dissolved and particulate organic carbon samples (DOC and POC) were collected following incubations. DOC samples were analyzed using a Shimadzu TOC-L total organic carbon analyzer (using the 680°C combustion catalytic oxidation method) (Levas et al 2015). POC filters (GF/F) were acid fumigated (Levas et al 2015) and combusted in an Elementar Vario EL Cube/Micro Cube elemental analyzer interfaced to a PDZ Europa 20-20 isotope ratio mass spectrometer at the stable isotope facility at the University of California - Davis. CHARtoc was calculated using methods modified from Levas et al (2015). CZAR and CHAR<sub>ZOO</sub> were summed to yield CTAR (Grottoli et al. 2014).</p>
<p>For tissue analyses, corals were frozen at -80 degrees C and samples were either ground of airbrushed for further analyses.</p>
<p><strong>Surface area.</strong> Surface area was measured on <em>Acropora millepora</em> using the wax-dipping technique (Veal et al. 2010) and on <em>Turbinaria reniformis</em> using the aluminum foil method (Marsh 1970).</p>
<p><strong>Tissue biomass.</strong> Ash free dry weight was calculated using methods in McLachlan et al. (2020) on ground growing tips of <em>A. millepora</em> and water-picked tissue slurry of <em>T. reniformis</em>.</p>
<p><strong>Chlorophyll <em>a</em>.</strong> <em>A. millepora</em> chlorophyll <em>a</em> was measured from tissue slurry using 100% acetone (Jeffrey &amp; Humphrey 1975). <em>T. reniformis</em> chlorophyll data was from Hoadley et al. (2016).</p>
<p><strong>Protein.</strong> Protein was measured using the bicinchoninic method (Smith et al. 1985) with bovine serum albumin as a standard (Pierce BCA Protein Assay Kit). This was carried out on air-brushed tissue slurry of <em>A. millepora</em> and water-picked and freeze-dried tissue slurry for <em>T. reniformis</em>. Protein was reported in Joules (Gnaiger &amp; Bitterlich, 1984) and standardized to total biomass of the sub-sample.</p>
<p><strong>Carbohydrates.</strong> Carbohydrates were measured using the phenol-sulfuric acid spectrophotometric method (Dubois et al. 1956) with glucose standards. This was carried out on air-brushed tissue slurry of <em>A. millepora</em> and ground <em>T. reniformis</em>. Carbohydrates were reported in Joules (Gnaiger &amp; Bitterlich, 1984) and standardized to total biomass of the sub-sample.</p>
<p><strong>Total lipids.</strong> Total lipids were measured using chloroform:methanol (2:1, v:v) with two KCl rinses (Baumann et al. 2014). This was carried out on air-brushed tissue slurry of <em>A. millepora</em> and water-piked and freeze-dried tissue slurry for <em>T. reniformis</em>. Total lipids were reported in Joules (Gnaiger &amp; Bitterlich, 1984) and standardized to total biomass of the sub-sample.</p>
<p><strong>δ<sup>15</sup>N isotopes (coral host, algal endosymbiont, whole tissue).</strong> Samples were prepped for isotopic analysis using a method modified from Hughes and Grottoli (2010). All <em>A. millepora</em> host and algal endosymbiont fractions, partitioned from airbrushed tissue slurry, were combusted in a Costech elemental analyzer and the resulting N<sub>2</sub> gas analyzed with a Thermo Finnigan Delta IV stable isotope ratio mass spectrometer (SIRMS) via a ConFlow open split interface in the Grottoli Stable Isotope Biogeochemistry Lab at The Ohio State University. All <em>T. reniformis</em> whole (freeze-dried tissue slurry) and algal endosymbiont fractions were combusted in an Elementar Vario EL Cube/Micro Cube elemental analyzer interfaced to a PDZ Europa 20-20 SIRMS at the stable isotope facility at University of California - Davis. Repeated measurements of an internal standard had an average SD ±0.08‰ δ<sup>15</sup>N. The δ<sup>15</sup>N values of both species are reported as the per mil deviation of the ratio of stable nitrogen isotopes <sup>15</sup>N:<sup>14</sup>N relative to air. Approximately 10% of <em>A. millepora</em> samples were run in duplicate with an average SD ± 0.21‰.</p>
<p>&nbsp;</p>
<p>A Euclidean distance-based resemblance matrix was constructed using normalized data of primary physiological variables: P, calcification, biomass, protein, and total lipids. Non-metric multi-dimensional scaling (NMDS) plots were generated to visualize relationships between each coral ramet across all treatments, for each species. Analysis of similarities (ANOSIM) was used to evaluate the degree of dissimilarity among treatments. All multivariate analyses were conducted using the software package Primer v6 (Clarke &amp; Gorley 2006). Prior to further statistical analysis, all data were tested for normality by a Shapiro-Wilk’s test and homogeneity of variance was assessed with plots of expected vs. residual values. Univariate three-way analysis of variance (ANOVA) was used to test the effects of temperature, <em>p</em>CO<sub>2</sub>, nutrients, and genotype on each measured variable for each species. Temperature was fixed with 2 levels (26.4°C, 29.8°C), <em>p</em>CO<sub>2</sub> fixed with 2 levels (401 μatm, 760 μatm), nutrients fixed with 2 levels (ambient, moderate). All univariate parametric statistics were generated using SAS software, Version 9.3 of the SAS System for Windows. Values of <em>p</em> ≤ 0.05 were considered significant.</p>
<p>Additional details of the statistical analysis are outlined in Dobson et al. 2020.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Costech elemental analyzer
Costech elemental analyzer
PI Supplied Instrument Name: Costech elemental analyzer PI Supplied Instrument Description:A. millepora δ15N samples measured on Costech elemental analyzer coupled to a Thermo Finnigan Delta IV stable isotope ratio mass spectrometer (EA-SIRMS). Instrument Name: Elemental Analyzer Instrument Short Name: Instrument Description: Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/
Elementar Vario EL Cube/Micro Cube elemental analyzer
Elementar Vario EL Cube/Micro Cube elemental analyzer
PI Supplied Instrument Name: Elementar Vario EL Cube/Micro Cube elemental analyzer PI Supplied Instrument Description:T. reniformis δ15N samples measured on Elementar Vario EL Cube/Micro Cube elemental analyzer interfaced to a PDZ Europa 20-20 stable isotope ratio mass spectrometer (EA-SIRMS). Instrument Name: Elemental Analyzer Instrument Short Name: Instrument Description: Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/
Thermo Finnigan Delta IV stable isotope ratio mass spectrometer
Thermo Finnigan Delta IV stable isotope ratio mass spectrometer
PI Supplied Instrument Name: Thermo Finnigan Delta IV stable isotope ratio mass spectrometer PI Supplied Instrument Description:A. millepora δ15N samples measured on Costech elemental analyzer coupled to a Thermo Finnigan Delta IV stable isotope ratio mass spectrometer (EA-SIRMS). Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
PDZ Europa 20-20 stable isotope ratio mass spectrometer
PDZ Europa 20-20 stable isotope ratio mass spectrometer
PI Supplied Instrument Name: PDZ Europa 20-20 stable isotope ratio mass spectrometer PI Supplied Instrument Description:T. reniformis δ15N samples measured on Elementar Vario EL Cube/Micro Cube elemental analyzer interfaced to a PDZ Europa 20-20 stable isotope ratio mass spectrometer (EA-SIRMS). Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Thermo Scientific Genesys
Thermo Scientific Genesys
PI Supplied Instrument Name: Thermo Scientific Genesys PI Supplied Instrument Description:A Thermo Scientific Genesys spectrophotometer was used to measure the intensity of light transmission at specific wavelengths for chlorophyll a, protein and carbohydrate samples of both species. Instrument Name: Spectrophotometer Instrument Short Name:Spectrophotometer Instrument Description: An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/
Shimadzu TOC-L total organic carbon analyzer
Shimadzu TOC-L total organic carbon analyzer
PI Supplied Instrument Name: Shimadzu TOC-L total organic carbon analyzer PI Supplied Instrument Description:DOC samples measured on Shimadzu TOC-L total organic carbon analyzer. Instrument Name: Total Organic Carbon Analyzer Instrument Short Name:TOC analyzer Instrument Description: A unit that accurately determines the carbon concentrations of organic compounds typically by detecting and measuring its combustion product (CO2). See description document at: http://bcodata.whoi.edu/LaurentianGreatLakes_Chemistry/bs116.pdf Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/