http://lod.bco-dmo.org/id/dataset/840078
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2021-02-08
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Measurements of chemoautotrophy in samples collected on cruise LMG1801 on R/V Laurence M. Gould from January to February 2018
2021-04-08
publication
2021-04-08
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2021-04-08
publication
https://doi.org/10.26008/1912/bco-dmo.840078.1
James T. Hollibaugh
University of Georgia
principalInvestigator
Brian N. Popp
University of Hawaiʻi at Mānoa
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Hollibaugh, J. T., Popp, B. N. (2021) Measurements of chemoautotrophy in samples collected on cruise LMG1801 on R/V Laurence M. Gould from January to February 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-04-08 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.840078.1 [access date]
LMG1801 Chemoautotrophy Dataset Description: Methods and Sampling: <p><strong>Sample Collection</strong>. Samples were collected on the Antarctic continental shelf and slope west of the Antarctic Peninsula within the PAL-LTER sampling domain (<a href="http://pal.lternet.edu/" target="_blank">http://pal.lternet.edu/</a>) during summer (cruise dates 30 Dec 2017 through 12 Feb 2018; sampling dates 5 Jan to 4 Feb 2018) from the ARSV Laurence M Gould (LMG 1801, PAL-LTER cruise 26, DOI: <a href="http://dx.doi.org/10.7284/907858" target="_blank">10.7284/907858</a>). Sampling focused on three or 4 depths at each station chosen to represent the Antarctic Surface Water (ASW, 0 -34 m depth), the Winter Water (WW, the water column temperature minimum, generally between 35 and 174 m) the Circumpolar Deep Water (CDW, 175-1000 m) and slope water (SLOPE, &gt;1000 m, generally ~10 m above the bottom at deep stations on the slope, 2500-3048m). Water samples were collected from Niskin bottles (General Oceanics Inc., Miami, FL, USA) into opaque 2 L HDPE plastic bottles or into aged, acid-washed, sample-rinsed 250 ml polycarbonate bottles (Nalge) completely filled (~270 mL) directly from Niskin bottles as soon as possible after the rosette was secured on deck. Subsequent processing took place in an adjacent laboratory.</p>
<p>Samples for DNA analysis were taken from the 2 L opaque HDPE bottles and were filtered under pressure through 0.22 um pore size Sterivex GVWP filters (EMD Millipore, Billerica, MA, USA) using a peristaltic pump. Residual seawater was expelled from the filter using a syringe filled with air, then ~1.8 ml of lysis buffer (0.75 M sucrose, 40 mM EDTA, 50 mM Tris, pH 8.3) was added to the filter capsule, which was capped and placed in a -20 °C freezer. The frozen samples were aggregated into Ziploc Freezer Bags and transferred to a -80 °C freezer for the remainder of the cruise and for shipping to the laboratory.</p>
<p>Two samples of the Sterivex filtrate (40 mL each into new 50 mL disposable centrifuge tubes, VWR, rinsed 3x with sample) were frozen immediately at -20 °C, then aggregated into Ziploc Freezer Bags and transferred to a -80 ° freezer for the remainder of the cruise and for shipping to the laboratory. These were used for subsequent determination of 1) urea concentration and 2) the natural abundance of <sup>15</sup>N in the nitrite plus nitrate pools (<sup>15</sup>NOₓ hereinafter). An additional sample of the Sterivex filtrate was stored in a polycarbonate bottle at 4 °C for subsequent onboard determination of ammonia concentration by the Holmes et al (1999) o-phthaldialdehyde method and nitrite concentration by the diazo-coupling method (Strickland and Parsons 1972). Technical difficulties encountered during onboard analysis resulted in the loss of ammonium and nitrite data for some samples.</p>
<p>Samples for DNA and chemical analyses were shipped on dry ice from Punta Arenas, Chile to the Hollibaugh laboratory at the University of Georgia. Upon arrival they were stored in a -80 °C freezer until analyzed. Samples for <sup>15</sup>N analysis were shipped on dry ice from Punta Arenas, Chile to the Popp laboratory at the University of Hawaii. Upon arrival they were stored in a -40 °C freezer until analyzed.</p>
<p><strong>Chemoautotropic production.</strong> Samples used for determining chemoautotrophic production were held in the dark at 0°C for no longer than 6 hours prior to being amended with <sup>14</sup>C bicarbonate. Chemoautotrophy was determined by measuring the incorporation into organic matter of <sup>14</sup>C supplied as NaH<sup>14</sup>CO₃. NaH<sup>14</sup>CO₃ (5 mCi) was diluted into 25 mL of MilliQ water made basic (pH ≈9) using NaOH. This stock solution (0.2 uCi/uL) was passed through a syringe filter (Acrodisk, 0.22 pore size) into a 30 mL polycarbonate bottle and stored at 4°C. Water from the sample depth was collected directly from the appropriate Niskin sampler into aged, acid-washed 250 mL screw-cap amber HDPE bottles (3 rinses) filled to the top (volume ~270 mL). Each experiment used two replicate treatments and a control bottle. Controls consisted of either 0.22 um filtered water from the Sterivex filtration of the same sample or of whole water that was incubated along with the <sup>14</sup>C amended treatment, except that no <sup>14</sup>C was added until immediately before filtering the set. Each bottle received ~ 20 uCi of NaH<sup>14</sup>CO₃ (100 uL of the working stock). Label was added in a darkened lab van illuminated with a dim, red-filtered light. Samples were mixed by inverting gently then placed in a water-ice bath contained in an ice chest wrapped in aluminum foil contained in a black 3 mil plastic garbage bag. Incubation temperature was maintained by adding ice as needed, which led to departures to above the desired incubation temperature for some stations. Water temperature in the bath was recorded at 5-minute time steps with HOBO TidBit loggers and these samples have been flagged. Lights were kept off except when working in the lab van, when red light was used to illuminate the working area.<br />
<br />
See the attached supplemental files for detailed data on the water bath temperature. "<a href="https://datadocs.bco-dmo.org/docs/305/Oxidation_of_Urea_N/data_docs/840078/LMG1801_Water_Bath_Temperature_Plot.pdf" target="_blank">LMG1801_Water_Bath_Temperature_Plot.pdf</a>" contains a figure plotting the bath temperature, the measured chemoautotrophy value for each station and depth, and the intervals of the incubations by station. "<a href="https://datadocs.bco-dmo.org/docs/305/Oxidation_of_Urea_N/data_docs/840078/Water_Bath_Temperature_Data.xlsx" target="_blank">Water_Bath_Temperature_Data.xlsx</a>" contains the data used to create this plot.</p>
<p>At the end of the incubation (~48 hours) the bottles were removed from the ice bath, triplicate samples of 100 uL were taken from the filtered control (or from one of the treatments in later experiments) and radioassayed to verify the amount of tracer added. The remaining sample and all of the treatment samples were filtered through 25 mm diameter, 0.22 um pore size membrane filters (GSWP Millipore) under dim red light. Filters were rinsed two times with filtered seawater, removed from the filter holder into scintillation vials, then 100 uL of 10% HCl was added to each vial, soaking the filter in the process. After 24 hr in the fume hood (uncapped) to allow excess water and acid to volatilize, vials received 4 mL of Ultima Gold scintillation cocktail, then were counted in a Perkin-Elmer LSC.</p>
Funding provided by NSF Office of Polar Programs (formerly NSF PLR) (NSF OPP) Award Number: OPP-1643466 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1643466
Funding provided by NSF Office of Polar Programs (formerly NSF PLR) (NSF OPP) Award Number: OPP-1643345 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1643345
completed
James T. Hollibaugh
University of Georgia
706-542-7671
Department of Marine Sciences, University of Georgia
Athens
GA
30602
USA
aquadoc@uga.edu
pointOfContact
Brian N. Popp
University of Hawaiʻi at Mānoa
808-956-6206
Department of Earth Sciences 1680 East-West Road
Honolulu
HI
96822
USA
popp@hawaii.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Event_Log_Number
Cast_Start_Time_GMT
Latitude
Longitude
Station_Description
LTER_Grid_Station
Depth
Treatment
Fraction_of_label_incorporated
Start_ISO_DateTime_UTC
End_ISO_DateTime_UTC
Incubation_Length
Midpoint_of_incubation
Chemoautotrophy
Incubation_Temp_Max
Incubation_Temp_Min
Incubation_Temp_Median
Incubation_Temp_Mean
Incubation_Temp_St_Dev
Perkin-Elmer LSC
Niskin bottles (General Oceanics Inc., Miami, FL, USA)
HOBO TidBit loggers
theme
None, User defined
event
ISO_DateTime_UTC
latitude
longitude
site description
station
depth
treatment
No BCO-DMO term
time_elapsed
water temperature at measurement depth
featureType
BCO-DMO Standard Parameters
Liquid Scintillation Counter
Niskin bottle
Temperature Logger
instrument
BCO-DMO Standard Instruments
LMG1801
service
Deployment Activity
Palmer LTER
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Chemoautotrophy in Antarctic Bacterioplankton Communities Supported by the Oxidation of Urea-derived Nitrogen
https://osprey.bco-dmo.org/project/775717
Collaborative Research: Chemoautotrophy in Antarctic Bacterioplankton Communities Supported by the Oxidation of Urea-derived Nitrogen
<p><em>NSF Award Abstract:</em><br />
Part 1: The project addresses fundamental questions regarding the role of nitrification (the conversion of ammonium to nitrate by a two-step process involving two different guilds of microorganisms: ammonia- and nitrite-oxidizers) in the Antarctic marine ecosystem. Specifically, the project seeks to evaluate the contribution of primary production supported by the energy in nitrogen compounds to the overall supply of organic carbon to the food web of the Southern Ocean. Previous measurements indicate that nitrification could contribute about 9% to primary production supporting the Antarctic food web on an annual basis, but those measurements did not include the additional production associated with nitrite oxidation. Additionally, the project will aim to determine the significance of the contribution of other sources of nitrogen, (specifically organic nitrogen and urea released by other organisms) to nitrification because these contributions may not be assessed by standard protocols. Such work will aid in better understanding the basis of the energy for the Antarctic marine ecosystems on an annual basis as well as better aid in understanding the energetics of the ecosystem in times and places where primary production based on light energy is limited (i.e. during the polar night or under sea ice cover).</p>
<p>This project will result in training a postdoctoral researcher and provide undergraduate students opportunities to gain hand-on experience with research on microbial geochemistry. The Palmer Long Term Ecological Research (LTER) activities have focused largely on the interaction between ocean climate and the marine food web affecting top predators. Relatively little effort has been devoted to studying processes related to the microbial geochemistry of nitrogen cycling, yet these are a major themes at other LTER sites. This work will contribute substantially to understanding an important aspect of nitrogen cycling and bacterioplankton production in the study area. The team will be working synergistically and be participating fully in the education and outreach efforts of the Palmer LTER, including making highlights of the findings available for posting to their project web site and participating in any special efforts they have in the area of outreach.</p>
<p>Part 2: The proposed work will quantify oxidation rates of 15N supplied as ammonium, urea and nitrite, allowing the estimation of the contribution of urea-derived N and complete nitrification (ammonia to nitrate) to chemoautotrophy and bacterioplankton production in Antarctic coastal waters. The project will compare these estimates to direct measurements of the incorporation of 14C into organic matter in the dark for an independent estimate of chemoautotrophy. The team aims to collect samples spanning the water column: from surface water (~10 m), winter water (50-100 m) and circumpolar deep water (>150 m); on a cruise surveying the continental shelf and slope west of the Antarctic Peninsula in the austral summer of 2018. Other samples will be taken to measure the concentrations of nitrate, nitrite, ammonia and urea, for qPCR analysis of the abundance of relevant microorganisms, and for studies of related processes. The project will rely on collaboration with the existing Palmer LTER to ensure that ancillary data (bacterioplankton abundance and production, chlorophyll, physical and chemical variables) will be available. The synergistic activities of this project along with the LTER activities will provide a unique opportunity to assess chemoautotrophy in context of the overall ecosystem's dynamics- including both primary and secondary production processes.</p>
Oxidation of Urea N
largerWorkCitation
project
eng; USA
oceans
Palmer LTER
-74.47352
-64.40628
-68.69457
-64.08913
2018-01-08
2018-02-04
Coastal, shelf and slope waters off the West Antarctic Peninsula, PAL-LTER sampling grid, Lawrence M Gould cruise 18-01
0
BCO-DMO catalogue of parameters from Measurements of chemoautotrophy in samples collected on cruise LMG1801 on R/V Laurence M. Gould from January to February 2018
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/845147.rdf
Name: Event_Log_Number
Units: unitless
Description: <p>Sequential numbers keyed to the bridge log of activities</p>
http://lod.bco-dmo.org/id/dataset-parameter/845148.rdf
Name: Cast_Start_Time_GMT
Units: unitless
Description: <p>Date and time of day for beginning CTD cast = sample collection; 24-hour clock; formatted to ISO8601 standard (UTC/GMT): YYYY-MM-DDThh:mmZ</p>
http://lod.bco-dmo.org/id/dataset-parameter/845149.rdf
Name: Latitude
Units: degrees North
Description: <p>Latitude in decimal degrees (negative values = South)</p>
http://lod.bco-dmo.org/id/dataset-parameter/845150.rdf
Name: Longitude
Units: degrees East
Description: <p>Longitude in decimal degrees (negative values = West)</p>
http://lod.bco-dmo.org/id/dataset-parameter/845151.rdf
Name: Station_Description
Units: unitless
Description: <p>PAL-LTER category for the station</p>
http://lod.bco-dmo.org/id/dataset-parameter/845152.rdf
Name: LTER_Grid_Station
Units: unitless
Description: <p>Station location on the PAL-LTER sampling grid (<a href="http://pal.lternet.edu">http://pal.lternet.edu</a>)</p>
http://lod.bco-dmo.org/id/dataset-parameter/845153.rdf
Name: Depth
Units: meters (m)
Description: <p>Depth sampled in meters</p>
http://lod.bco-dmo.org/id/dataset-parameter/845154.rdf
Name: Treatment
Units: unitless
Description: <p>Identifies manipulation experiments or comparisons</p>
http://lod.bco-dmo.org/id/dataset-parameter/845155.rdf
Name: Fraction_of_label_incorporated
Units: unitless
Description: <p>Fraction of the added 14C that was incorporated into organic matter</p>
http://lod.bco-dmo.org/id/dataset-parameter/845156.rdf
Name: Start_ISO_DateTime_UTC
Units: unitless
Description: <p>Time at which the incubation was initiated by adding 14C to the sample; 24-hour clock; formatted to formatted to ISO8601 standard (UTC/GMT): YYYY-MM-DDThh:mmZ</p>
http://lod.bco-dmo.org/id/dataset-parameter/845157.rdf
Name: End_ISO_DateTime_UTC
Units: unitless
Description: <p>Time at which the incubation was terminated by filtration; 24-hour clock; formatted to formatted to ISO8601 standard (UTC/GMT): YYYY-MM-DDThh:mmZ</p>
http://lod.bco-dmo.org/id/dataset-parameter/845158.rdf
Name: Incubation_Length
Units: days
Description: <p>Duration of the incubation in days</p>
http://lod.bco-dmo.org/id/dataset-parameter/845159.rdf
Name: Midpoint_of_incubation
Units: decimal days
Description: <p>Julian Day of 2018, as a decimal day (GMT)</p>
http://lod.bco-dmo.org/id/dataset-parameter/845160.rdf
Name: Chemoautotrophy
Units: nanomoles C per liter per day (nmol C L-1 d-1)
Description: <p>Carbon fixation rate, nmol C L-1 d-1</p>
http://lod.bco-dmo.org/id/dataset-parameter/845161.rdf
Name: Incubation_Temp_Max
Units: degrees Celsius
Description: <p>Maximum temperature recorded in the incubator during an incubation</p>
http://lod.bco-dmo.org/id/dataset-parameter/845162.rdf
Name: Incubation_Temp_Min
Units: degrees Celsius
Description: <p>Minimum temperature recorded in the incubator during an incubation</p>
http://lod.bco-dmo.org/id/dataset-parameter/845163.rdf
Name: Incubation_Temp_Median
Units: degrees Celsius
Description: <p>Median temperature recorded in the incubator during an incubation</p>
http://lod.bco-dmo.org/id/dataset-parameter/845164.rdf
Name: Incubation_Temp_Mean
Units: degrees Celsius
Description: <p>Mean temperature recorded in the incubator during an incubation</p>
http://lod.bco-dmo.org/id/dataset-parameter/845165.rdf
Name: Incubation_Temp_St_Dev
Units: degrees Celsius
Description: <p>Standard Deviation of the temperature recorded in the incubator during an incubation</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
6889
https://darchive.mblwhoilibrary.org/bitstream/1912/26921/1/dataset-840078_chemoautotrophy__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.840078.1
download
onLine
dataset
<p><strong>Sample Collection</strong>. Samples were collected on the Antarctic continental shelf and slope west of the Antarctic Peninsula within the PAL-LTER sampling domain (<a href="http://pal.lternet.edu/" target="_blank">http://pal.lternet.edu/</a>) during summer (cruise dates 30 Dec 2017 through 12 Feb 2018; sampling dates 5 Jan to 4 Feb 2018) from the ARSV Laurence M Gould (LMG 1801, PAL-LTER cruise 26, DOI: <a href="http://dx.doi.org/10.7284/907858" target="_blank">10.7284/907858</a>). Sampling focused on three or 4 depths at each station chosen to represent the Antarctic Surface Water (ASW, 0 -34 m depth), the Winter Water (WW, the water column temperature minimum, generally between 35 and 174 m) the Circumpolar Deep Water (CDW, 175-1000 m) and slope water (SLOPE, &gt;1000 m, generally ~10 m above the bottom at deep stations on the slope, 2500-3048m). Water samples were collected from Niskin bottles (General Oceanics Inc., Miami, FL, USA) into opaque 2 L HDPE plastic bottles or into aged, acid-washed, sample-rinsed 250 ml polycarbonate bottles (Nalge) completely filled (~270 mL) directly from Niskin bottles as soon as possible after the rosette was secured on deck. Subsequent processing took place in an adjacent laboratory.</p>
<p>Samples for DNA analysis were taken from the 2 L opaque HDPE bottles and were filtered under pressure through 0.22 um pore size Sterivex GVWP filters (EMD Millipore, Billerica, MA, USA) using a peristaltic pump. Residual seawater was expelled from the filter using a syringe filled with air, then ~1.8 ml of lysis buffer (0.75 M sucrose, 40 mM EDTA, 50 mM Tris, pH 8.3) was added to the filter capsule, which was capped and placed in a -20 °C freezer. The frozen samples were aggregated into Ziploc Freezer Bags and transferred to a -80 °C freezer for the remainder of the cruise and for shipping to the laboratory.</p>
<p>Two samples of the Sterivex filtrate (40 mL each into new 50 mL disposable centrifuge tubes, VWR, rinsed 3x with sample) were frozen immediately at -20 °C, then aggregated into Ziploc Freezer Bags and transferred to a -80 ° freezer for the remainder of the cruise and for shipping to the laboratory. These were used for subsequent determination of 1) urea concentration and 2) the natural abundance of <sup>15</sup>N in the nitrite plus nitrate pools (<sup>15</sup>NOₓ hereinafter). An additional sample of the Sterivex filtrate was stored in a polycarbonate bottle at 4 °C for subsequent onboard determination of ammonia concentration by the Holmes et al (1999) o-phthaldialdehyde method and nitrite concentration by the diazo-coupling method (Strickland and Parsons 1972). Technical difficulties encountered during onboard analysis resulted in the loss of ammonium and nitrite data for some samples.</p>
<p>Samples for DNA and chemical analyses were shipped on dry ice from Punta Arenas, Chile to the Hollibaugh laboratory at the University of Georgia. Upon arrival they were stored in a -80 °C freezer until analyzed. Samples for <sup>15</sup>N analysis were shipped on dry ice from Punta Arenas, Chile to the Popp laboratory at the University of Hawaii. Upon arrival they were stored in a -40 °C freezer until analyzed.</p>
<p><strong>Chemoautotropic production.</strong> Samples used for determining chemoautotrophic production were held in the dark at 0°C for no longer than 6 hours prior to being amended with <sup>14</sup>C bicarbonate. Chemoautotrophy was determined by measuring the incorporation into organic matter of <sup>14</sup>C supplied as NaH<sup>14</sup>CO₃. NaH<sup>14</sup>CO₃ (5 mCi) was diluted into 25 mL of MilliQ water made basic (pH ≈9) using NaOH. This stock solution (0.2 uCi/uL) was passed through a syringe filter (Acrodisk, 0.22 pore size) into a 30 mL polycarbonate bottle and stored at 4°C. Water from the sample depth was collected directly from the appropriate Niskin sampler into aged, acid-washed 250 mL screw-cap amber HDPE bottles (3 rinses) filled to the top (volume ~270 mL). Each experiment used two replicate treatments and a control bottle. Controls consisted of either 0.22 um filtered water from the Sterivex filtration of the same sample or of whole water that was incubated along with the <sup>14</sup>C amended treatment, except that no <sup>14</sup>C was added until immediately before filtering the set. Each bottle received ~ 20 uCi of NaH<sup>14</sup>CO₃ (100 uL of the working stock). Label was added in a darkened lab van illuminated with a dim, red-filtered light. Samples were mixed by inverting gently then placed in a water-ice bath contained in an ice chest wrapped in aluminum foil contained in a black 3 mil plastic garbage bag. Incubation temperature was maintained by adding ice as needed, which led to departures to above the desired incubation temperature for some stations. Water temperature in the bath was recorded at 5-minute time steps with HOBO TidBit loggers and these samples have been flagged. Lights were kept off except when working in the lab van, when red light was used to illuminate the working area.<br />
<br />
See the attached supplemental files for detailed data on the water bath temperature. "<a href="https://datadocs.bco-dmo.org/docs/305/Oxidation_of_Urea_N/data_docs/840078/LMG1801_Water_Bath_Temperature_Plot.pdf" target="_blank">LMG1801_Water_Bath_Temperature_Plot.pdf</a>" contains a figure plotting the bath temperature, the measured chemoautotrophy value for each station and depth, and the intervals of the incubations by station. "<a href="https://datadocs.bco-dmo.org/docs/305/Oxidation_of_Urea_N/data_docs/840078/Water_Bath_Temperature_Data.xlsx" target="_blank">Water_Bath_Temperature_Data.xlsx</a>" contains the data used to create this plot.</p>
<p>At the end of the incubation (~48 hours) the bottles were removed from the ice bath, triplicate samples of 100 uL were taken from the filtered control (or from one of the treatments in later experiments) and radioassayed to verify the amount of tracer added. The remaining sample and all of the treatment samples were filtered through 25 mm diameter, 0.22 um pore size membrane filters (GSWP Millipore) under dim red light. Filters were rinsed two times with filtered seawater, removed from the filter holder into scintillation vials, then 100 uL of 10% HCl was added to each vial, soaking the filter in the process. After 24 hr in the fume hood (uncapped) to allow excess water and acid to volatilize, vials received 4 mL of Ultima Gold scintillation cocktail, then were counted in a Perkin-Elmer LSC.</p>
Specified by the Principal Investigator(s)
- renamed fields to comply with BCO-DMO naming conventions;
- converted start and end date/time fields to ISO8601 format;
- 2021-03-16: revised/updated the Methods & Sampling section of the metadata;
- 2021-04-08: replaced data file with copy received on 2021-03-18.
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Perkin-Elmer LSC
Perkin-Elmer LSC
PI Supplied Instrument Name: Perkin-Elmer LSC Instrument Name: Liquid Scintillation Counter Instrument Short Name:LSC Instrument Description: Liquid scintillation counting is an analytical technique which is defined by the incorporation of the radiolabeled analyte into uniform distribution with a liquid chemical medium capable of converting the kinetic energy of nuclear emissions into light energy. Although the liquid scintillation counter is a sophisticated laboratory counting system used to quantify the activity of particulate emitting (ß and a) radioactive samples, it can also detect the auger electrons emitted from 51Cr and 125I samples.
Liquid scintillation counters are instruments assaying alpha and beta radiation by quantitative detection of visible light produced by the passage of rays or particles through a suitable scintillant incorporated into the sample. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB21/
Niskin bottles (General Oceanics Inc., Miami, FL, USA)
Niskin bottles (General Oceanics Inc., Miami, FL, USA)
PI Supplied Instrument Name: Niskin bottles (General Oceanics Inc., Miami, FL, USA) Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
HOBO TidBit loggers
HOBO TidBit loggers
PI Supplied Instrument Name: HOBO TidBit loggers Instrument Name: Temperature Logger Instrument Short Name: Instrument Description: Records temperature data over a period of time.
Cruise: LMG1801
LMG1801
ARSV Laurence M. Gould
Community Standard Description
International Council for the Exploration of the Sea
ARSV Laurence M. Gould
vessel
ARSV Laurence M. Gould
Community Standard Description
International Council for the Exploration of the Sea
ARSV Laurence M. Gould
vessel