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            <gco:CharacterString>Cite this dataset as: Steinmuller, H. E., White, J. R., Cook, R. L., Xue, Z., Chambers, L. G. (2021) Soil physicochemical properties of coastal wetland soil cores collected in June 2018 from Barataria Bay, Louisiana. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-02-10 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.840246.1 [access date]</gco:CharacterString>
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        <gco:CharacterString>Soil physicochemical properties of wetland soil cores (Barataria Bay, LA) Dataset Description:  Methods and Sampling: &amp;lt;p&amp;gt;Nine coastal wetland soil cores were collected in June 2018 from Barataria Bay, Louisiana, a shallow open water basin located west of the Mississippi River Delta.&amp;amp;nbsp;&amp;amp;nbsp;Soil cores were collected along three transects, roughly 1 meter apart, that consisted of three points:&amp;amp;nbsp; the coastal fringe (0 m inland), 1 meter inland, and 2 meters inland. Soil cores were collected in polycarbonate&amp;amp;nbsp;tubes via the push core method to a depth of 150 cm, and field-extruded into 15 separate 10-cm intervals. Soils were stored&amp;amp;nbsp; in polyethylene bags on ice and immediately transported back to the laboratory, where they were kept at 4 °C until sample analysis was complete.&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;This dataset includes analyses of&amp;amp;nbsp;biogeochemical properties, organic matter fractionation, and stable isotope signatures.&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Moisture Content and Bulk Density&amp;lt;/strong&amp;gt;: A subsample of soil was dried using a gravimetric oven at 70°C for 3 days or until a constant weight was achieved. Dried soils were ground using a SPEX Sample Prep 8000M Mixer/Mill (Metuchen, NJ).&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Total Carbon and Nitrogen content:&amp;amp;nbsp;&amp;lt;/strong&amp;gt;&amp;amp;nbsp;%C and %N were&amp;amp;nbsp; determined using a Vario Micro Cube CHNS Analyzer on dried, ground subsamples.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Organic Matter Content:&amp;lt;/strong&amp;gt;&amp;amp;nbsp;Dried, ground sub- samples were used to determine percent organic matter using the loss- on-ignition method, where soils were burned at 550°C in a muffle furnace for a total of 3 hours&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Extractable Nitrate, Extractable Ammonium, Extractable&amp;amp;nbsp;Soluble Reactive Phosphorus:&amp;amp;nbsp; &amp;lt;/strong&amp;gt;2.5 grams of wet soil (both from the field and from the bottle incubation) were placed into 40 mL centrifuge tubes and 25 mL of 2 M KCl was added. Samples were then shaken continuously on an orbital shaker for 1 hour at 25 °C and 150 rpm, then centrifuged for 10 minutes at 10 °C and 5000 rpm.&amp;amp;nbsp; Following the centrifuge step, samples were immediately filtered through Supor 0.45 μM filters and acidified with double distilled H2SO4 to a pH of &amp;amp;lt; 2 for preservation. Samples of Extractable nutrients&amp;amp;nbsp; were then analyzed using an AQ2 Automated Discrete Analyzer (Seal Analytical, Mequon, WI, EPA methods 231-A Rev.0, 210-A Rev.1, and 204-A Rev.0).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Carbon and Nitrogen isotopes: &amp;lt;/strong&amp;gt;Stable isotope analysis was performed at the Stable Isotope Mass Spectroscopy Laboratory, Department of Geological Sciences at the University of Florida.&amp;amp;nbsp; Dried, ground subsamples from only the 1 meter inland cores were initially combusted on a Carlo Erba NA1500 CNS elemental analyzer.&amp;amp;nbsp; Following the removal of oxygen and water from the sample gas, the stream was passed through a 0.7 m GC column (120 °C), which separated the N2 gas from CO2.&amp;amp;nbsp; Effluent then passed into a ConFlo II system, and into a Thermo Electron Delta V Advantage isotope ratio mass spectrometer, where sample gas was measured in relation to laboratory reference gases.&amp;amp;nbsp; Carbon isotope results are expressed in relation to Vienna PDB, in standard delta notation.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Cellulose, Hemicellulose, and Refractory Carbon:&amp;amp;nbsp;&amp;lt;/strong&amp;gt;Dried, ground subsamples were subjected to sequential extraction with H2SO4, following Rovira and Vallejo (2002) and Oades et al. (1970), with modifications.&amp;amp;nbsp; The first fraction is referred to hereafter as Labile Pool 1 (LP1) and consists of either plant- or microbially-derived non-cellulosic polysaccharides, including hemicellulose.&amp;amp;nbsp; Labile Pool 1 was extracted by adding 20 mL of 5 N H2SO4 into a 50 mL flask containing 0.5 grams of soil.&amp;amp;nbsp; The solution was heated for 30 minutes at 105 °C and subsequently allowed to cool.&amp;amp;nbsp; Samples were then filtered through Whatman #41 filters to separate particulates from the solution, and then diluted to a final volume of 50 mL.&amp;amp;nbsp; Labile Pool 2 (LP2), which consists of cellulose, was determined by adding 2 mL of 26 N H2SO4 to 0.5 grams of dried, ground soil.&amp;amp;nbsp; Samples were shaken at 100 rpm for 16 hours, then diluted to a final concentration of 2 N H2SO4 with deionized water.&amp;amp;nbsp; Samples were heated for 3 hours at 105°C, then filtered in the same manner as LP1.&amp;amp;nbsp; Determinations of LP1 and LP2 concentrations were conducted by use of a Shimadzu TOC-L (Shimadzu, Kyoto, Japan).&amp;amp;nbsp; The refractory pool was calculated as total soil C minus the sum of the labile pools.&amp;amp;nbsp; All pools were normalized to total soil C content.&amp;amp;nbsp;&amp;amp;nbsp;&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/670519.rdf" xlink:title="OCE-1635837" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1635837 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1635837</gmx:Anchor>
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Coastal Louisiana is currently experiencing net sea level rise at rates higher than most of the world's coastlines and within the global range predicted to occur in the next 65 - 85 years, making Louisiana an ideal site to study potential future impacts of rising sea level on coastal systems. This project will use field collection and controlled tank experiments to study the changing organic carbon cycle resulting from erosion of marsh soils along with its impact on associated biogeochemical processes. The hypothesis tested in this study is that the majority of eroded soil organic carbon is converted to carbon dioxide (CO2) and released to the atmosphere, representing an addition to the anthropogenic input of CO2. This process has not been quantified and could be an important missing component in predictive models of atmospheric CO2 changes. While this process may be of only regional importance today in comparison to other sources of CO2, this study of the Louisiana coast will greatly enhance our full understanding of the potential impacts on the global carbon cycle that may result from coastal erosion as global sea level continues to rise.&lt;/p&gt;
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	Name: Distance
	Units: meters (m)
	Description: &lt;p&gt;Distance from marsh edge&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/851568.rdf
	Name: Rep
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http://lod.bco-dmo.org/id/dataset-parameter/851569.rdf
	Name: Depth
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http://lod.bco-dmo.org/id/dataset-parameter/851570.rdf
	Name: Moisture_pcnt
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http://lod.bco-dmo.org/id/dataset-parameter/851571.rdf
	Name: Bulk_Density
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http://lod.bco-dmo.org/id/dataset-parameter/851572.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/851573.rdf
	Name: d15N
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http://lod.bco-dmo.org/id/dataset-parameter/851574.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/851575.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/851576.rdf
	Name: C_pcnt
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http://lod.bco-dmo.org/id/dataset-parameter/851577.rdf
	Name: Extractable_Nitrate
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http://lod.bco-dmo.org/id/dataset-parameter/851578.rdf
	Name: Extractable_SRP
	Units: milligrams per kilogram (mg kg-1)
	Description: &lt;p&gt;Concentration of Extractable Soluble Reactive Phosphorous within a soil sample&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/851579.rdf
	Name: Extractable_Ammonium
	Units: milligrams per kilogram (mg kg-1)
	Description: &lt;p&gt;Concentration of Extractable Ammonium (NH4+) within a soil sample&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/851580.rdf
	Name: Hemicellulose
	Units: milligrams per kilogram (mg kg-1)
	Description: &lt;p&gt;Concentration of Hemicellulose within soil&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/851581.rdf
	Name: Cellulose
	Units: milligrams per kilogram (mg kg-1)
	Description: &lt;p&gt;Concentration of Cellulose within soil&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/851582.rdf
	Name: Recalcitrant_Fraction
	Units: milligrams per kilogram (mg kg-1)
	Description: &lt;p&gt;Concentration of Refractory Carbon within soil&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/851583.rdf
	Name: Latitude
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http://lod.bco-dmo.org/id/dataset-parameter/851584.rdf
	Name: Longitude
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	Description: &lt;p&gt;Longitude of sampling location (West is negative)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/851585.rdf
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                <gco:CharacterString>&amp;lt;p&amp;gt;Nine coastal wetland soil cores were collected in June 2018 from Barataria Bay, Louisiana, a shallow open water basin located west of the Mississippi River Delta.&amp;amp;nbsp;&amp;amp;nbsp;Soil cores were collected along three transects, roughly 1 meter apart, that consisted of three points:&amp;amp;nbsp; the coastal fringe (0 m inland), 1 meter inland, and 2 meters inland. Soil cores were collected in polycarbonate&amp;amp;nbsp;tubes via the push core method to a depth of 150 cm, and field-extruded into 15 separate 10-cm intervals. Soils were stored&amp;amp;nbsp; in polyethylene bags on ice and immediately transported back to the laboratory, where they were kept at 4 °C until sample analysis was complete.&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;This dataset includes analyses of&amp;amp;nbsp;biogeochemical properties, organic matter fractionation, and stable isotope signatures.&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Moisture Content and Bulk Density&amp;lt;/strong&amp;gt;: A subsample of soil was dried using a gravimetric oven at 70°C for 3 days or until a constant weight was achieved. Dried soils were ground using a SPEX Sample Prep 8000M Mixer/Mill (Metuchen, NJ).&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Total Carbon and Nitrogen content:&amp;amp;nbsp;&amp;lt;/strong&amp;gt;&amp;amp;nbsp;%C and %N were&amp;amp;nbsp; determined using a Vario Micro Cube CHNS Analyzer on dried, ground subsamples.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Organic Matter Content:&amp;lt;/strong&amp;gt;&amp;amp;nbsp;Dried, ground sub- samples were used to determine percent organic matter using the loss- on-ignition method, where soils were burned at 550°C in a muffle furnace for a total of 3 hours&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Extractable Nitrate, Extractable Ammonium, Extractable&amp;amp;nbsp;Soluble Reactive Phosphorus:&amp;amp;nbsp; &amp;lt;/strong&amp;gt;2.5 grams of wet soil (both from the field and from the bottle incubation) were placed into 40 mL centrifuge tubes and 25 mL of 2 M KCl was added. Samples were then shaken continuously on an orbital shaker for 1 hour at 25 °C and 150 rpm, then centrifuged for 10 minutes at 10 °C and 5000 rpm.&amp;amp;nbsp; Following the centrifuge step, samples were immediately filtered through Supor 0.45 μM filters and acidified with double distilled H2SO4 to a pH of &amp;amp;lt; 2 for preservation. Samples of Extractable nutrients&amp;amp;nbsp; were then analyzed using an AQ2 Automated Discrete Analyzer (Seal Analytical, Mequon, WI, EPA methods 231-A Rev.0, 210-A Rev.1, and 204-A Rev.0).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Carbon and Nitrogen isotopes: &amp;lt;/strong&amp;gt;Stable isotope analysis was performed at the Stable Isotope Mass Spectroscopy Laboratory, Department of Geological Sciences at the University of Florida.&amp;amp;nbsp; Dried, ground subsamples from only the 1 meter inland cores were initially combusted on a Carlo Erba NA1500 CNS elemental analyzer.&amp;amp;nbsp; Following the removal of oxygen and water from the sample gas, the stream was passed through a 0.7 m GC column (120 °C), which separated the N2 gas from CO2.&amp;amp;nbsp; Effluent then passed into a ConFlo II system, and into a Thermo Electron Delta V Advantage isotope ratio mass spectrometer, where sample gas was measured in relation to laboratory reference gases.&amp;amp;nbsp; Carbon isotope results are expressed in relation to Vienna PDB, in standard delta notation.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Cellulose, Hemicellulose, and Refractory Carbon:&amp;amp;nbsp;&amp;lt;/strong&amp;gt;Dried, ground subsamples were subjected to sequential extraction with H2SO4, following Rovira and Vallejo (2002) and Oades et al. (1970), with modifications.&amp;amp;nbsp; The first fraction is referred to hereafter as Labile Pool 1 (LP1) and consists of either plant- or microbially-derived non-cellulosic polysaccharides, including hemicellulose.&amp;amp;nbsp; Labile Pool 1 was extracted by adding 20 mL of 5 N H2SO4 into a 50 mL flask containing 0.5 grams of soil.&amp;amp;nbsp; The solution was heated for 30 minutes at 105 °C and subsequently allowed to cool.&amp;amp;nbsp; Samples were then filtered through Whatman #41 filters to separate particulates from the solution, and then diluted to a final volume of 50 mL.&amp;amp;nbsp; Labile Pool 2 (LP2), which consists of cellulose, was determined by adding 2 mL of 26 N H2SO4 to 0.5 grams of dried, ground soil.&amp;amp;nbsp; Samples were shaken at 100 rpm for 16 hours, then diluted to a final concentration of 2 N H2SO4 with deionized water.&amp;amp;nbsp; Samples were heated for 3 hours at 105°C, then filtered in the same manner as LP1.&amp;amp;nbsp; Determinations of LP1 and LP2 concentrations were conducted by use of a Shimadzu TOC-L (Shimadzu, Kyoto, Japan).&amp;amp;nbsp; The refractory pool was calculated as total soil C minus the sum of the labile pools.&amp;amp;nbsp; All pools were normalized to total soil C content.&amp;amp;nbsp;&amp;amp;nbsp;&amp;lt;/p&amp;gt;</gco:CharacterString>
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All statistical analysis was performed in R (R Institute for Statistical Computing, Vienna, Austria) using RStudio (RStudio Inc., Boston, MA, USA). The Shapiro-Wilk test was used to verify assumptions of normality, and a logarithmic transformation was performed on all datasets. &amp;amp;nbsp;Levene’s test was used to determine homogeneity of variance. A linear mixed-effect model (package ‘lmer’) was used to test the following predictor variables: depth, distance inland, and the interaction of depth and distance inland on the samples collected from the marsh. Transect was included as a random effect. Distance inland was found to be a non-significant predictor variable for all parameters except for extractable NH4+. As such, distance inland and the interaction of depth and distance was removed as a predictor variable from models except extractable NH4+. Isotopic determinations and quantitative PCR analysis was performed exclusively on the three replicate cores taken 1 meter inland, and thus depth was the only predictor variable tested for those parameters. Following determination of significance within one of the predictor variables, the package ‘lsmeans’ was used for post-hoc pairwise comparisons using the Tukey method.&amp;lt;/p&amp;gt;

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Examples: Finnigan MATConFlo II, ThermoScientific ConFlo IV, and Picarro Caddy.

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Developed by Shimadzu, the 680 degree C combustion catalytic oxidation method is now used worldwide. One of its most important features is the capacity to efficiently oxidize hard-to-decompose organic compounds, including insoluble and macromolecular organic compounds. The 680 degree C combustion catalytic oxidation method has been adopted for the TOC-L series.

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