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            <gco:CharacterString>Cite this dataset as: Steinmuller, H. E., White, J. R., Cook, R. L., Xue, Z., Chambers, L. G. (2021) Nutrient properties of coastal wetland soil cores collected in June 2018 from Barataria Bay, Louisiana. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-02-10 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.840293.1 [access date]</gco:CharacterString>
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        <gco:CharacterString>Methods and Sampling: &amp;lt;p&amp;gt;Nine coastal wetland soil cores were collected in June 2018 from Barataria Bay, Louisiana, a shallow open water basin located west of the Mississippi River Delta.&amp;amp;nbsp;&amp;amp;nbsp;Soil cores were collected along three transects, roughly 1 meter apart, that consisted of three points:&amp;amp;nbsp; the coastal fringe (0 m inland), 1 meter inland, and 2 meters inland. Soil cores were collected in polycarbonate&amp;amp;nbsp;tubes via the push core method to a depth of 150 cm, and field-extruded into 15 separate 10-cm intervals. Soils were stored&amp;amp;nbsp; in polyethylene bags on ice and immediately transported back to the laboratory, where they were kept at 4 °C until sample analysis was complete.&amp;amp;nbsp;&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;This dataset includes analyses of nutrients.&amp;lt;/p&amp;gt;

&amp;lt;p dir=&amp;quot;ltr&amp;quot;&amp;gt;Samples of 2.5 grams of wet soil (both from the field and from the bottle incubation) were placed into 40 mL centrifuge tubes to which 25 mL of 2 M KCl was added.&amp;amp;nbsp; Samples were then shaken continuously on an orbital shaker for 1 hour at 25 °C and 150 rpm, then centrifuged for 10 minutes at 10 °C and 5000 rpm. Following the centrifuge step, samples were immediately filtered through Supor 0.45 μM filters and acidified with double distilled H2SO4 to a pH of &amp;amp;lt; 2 for preservation. Extractable nutrients (nitrate, soluble reactive phosphorus, and ammonium) were then analyzed with an AQ2 Automated Discrete Analyzer (Seal Analytical, Mequon, WI) and using&amp;amp;nbsp;EPA methods 231-A Rev.0, 210-A Rev.1, and 204-A Rev.0.&amp;amp;nbsp;&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/670519.rdf" xlink:title="OCE-1635837" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1635837 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1635837</gmx:Anchor>
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            <gmx:Anchor xlink:href="http://orcid.org/0000-0001-6432-8038" xlink:actuate="onRequest">Lisa G. Chambers</gmx:Anchor>
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            <gmx:Anchor xlink:href="https://ror.org/036nfer12" xlink:title="ROR ID" xlink:actuate="onRequest">University of Central Florida</gmx:Anchor>
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	Units: centimeters (cm)
	Description: &lt;p&gt;Soil depth below the surface.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/840538.rdf
	Name: Extractable_Nitrate
	Units: miligrams per kilometer (mg kg-1)
	Description: &lt;p&gt;Concentration of extractable nitrate within a soil sample.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/840539.rdf
	Name: Extractable_SRP
	Units: miligrams per kilometer (mg kg-1)
	Description: &lt;p&gt;Concentration of extractable soluble reactive phosphorous within a soil sample.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/840540.rdf
	Name: Extractable_Ammonium
	Units: miligrams per kilometer (mg kg-1)
	Description: &lt;p&gt;Concentration of extractable ammonium within a soil sample.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/840541.rdf
	Name: Latitude
	Units: decimal degrees
	Description: &lt;p&gt;Latitude&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/840542.rdf
	Name: Longitude
	Units: decimal degress
	Description: &lt;p&gt;Longitude (west is negative)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/851549.rdf
	Name: Date_collected
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&amp;lt;p&amp;gt;This dataset includes analyses of nutrients.&amp;lt;/p&amp;gt;

&amp;lt;p dir=&amp;quot;ltr&amp;quot;&amp;gt;Samples of 2.5 grams of wet soil (both from the field and from the bottle incubation) were placed into 40 mL centrifuge tubes to which 25 mL of 2 M KCl was added.&amp;amp;nbsp; Samples were then shaken continuously on an orbital shaker for 1 hour at 25 °C and 150 rpm, then centrifuged for 10 minutes at 10 °C and 5000 rpm. Following the centrifuge step, samples were immediately filtered through Supor 0.45 μM filters and acidified with double distilled H2SO4 to a pH of &amp;amp;lt; 2 for preservation. Extractable nutrients (nitrate, soluble reactive phosphorus, and ammonium) were then analyzed with an AQ2 Automated Discrete Analyzer (Seal Analytical, Mequon, WI) and using&amp;amp;nbsp;EPA methods 231-A Rev.0, 210-A Rev.1, and 204-A Rev.0.&amp;amp;nbsp;&amp;lt;/p&amp;gt;</gco:CharacterString>
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All statistical analysis was performed in R (R Institute for Statistical Computing, Vienna, Austria) using RStudio (RStudio Inc., Boston, MA, USA). The Shapiro-Wilk test was used to verify assumptions of normality, and a logarithmic transformation was performed on all datasets. &amp;amp;nbsp;Levene’s test was used to determine homogeneity of variance. A linear mixed-effect model (package ‘lmer’) was used to test the following predictor variables: depth, distance inland, and the interaction of depth and distance inland on the samples collected from the marsh. Transect was included as a random effect. Distance inland was found to be a non-significant predictor variable for all parameters except for extractable NH4+. As such, distance inland and the interaction of depth and distance was removed as a predictor variable from models except extractable NH4+. Isotopic determinations and quantitative PCR analysis was performed exclusively on the three replicate cores taken 1 m inland, and thus depth was the only predictor variable tested for those parameters. Following determination of significance within one of the predictor variables, the package ‘lsmeans’ was used for post-hoc pairwise comparisons using the Tukey method.&amp;lt;/p&amp;gt;

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