<div><p>Krill were collected from surface waters (upper 50 m) at each sampling station during the nighttime using a 60-cm diameter Bongo frame equipped with black 335-μm mesh nets and non-filtering cod ends towed for <10 min at 2-3 kts. Healthy female <em>E. pacifica</em> (obvious ovary) were identified under a microscope, kept chilled (10-14 °C) at all times, and were gently transferred using broad spoons to prevent damage.</p></div>
Krill physiology - In situ conditions
<div><p>Krill were measured for total length (from behind the eye to the end of the telson) at 6X using a calibrated eyepiece reticle then flash frozen individually in liquid nitrogen. Total length was converted to dry weight (DW) using a published length-weight regression (Feinberg et al. 2007).</p>
<p>Protein content was determined according to the bicinchoninic acid (BCA) method (Smith et al. 1985) using a Pierce BCA Protein Assay Kit (Thermo Scientific).</p>
<p>ETS activity was assayed using the method of Owens and King (1975), as modified by Gómez et al. (1996), and adapted for a 96-well plate. Assays and blanks were measured in triplicate at 25 °C, calculated according to Packard and Christensen (2004), and corrected to <em>in situ</em> temperatures (depth integrated) using the Arrhenius equation with an activation energy of 15 kcal mol-1 (Packard et al. 1975) and standardized to protein specific activity, spETS.</p>
<p>AARS was measured following the method of Yebra and Hernadez-León (2004), modified by Yebra et al. (2011), and adapted for a 96-well plate (Yebra et al. 2017). All activities were corrected to <em>in situ</em> temperatures with the Arrhenius equation using an activation energy of 8.57 kcal mol-1 (Yebra et al. 2005) and standardized to protein specific activity, spAARS. spAARS_1 was measured with the NADH Blank and calculated as described by Mclaskey et al. (2020). For each sample, assays and NADH Blanks were measured in triplicate.</p>
<p>Oxygen consumption of individual krill was measured at 12 °C by closed-cell respirometry in 22-mL vials containing optical oxygen sensors (PSt7 PreSens) and a 12-mm magnetic stir bar separated from the animal by 200-μm mesh. Measurements were taken every 15 minutes for 2 hours. Krill were kept in filtered seawater to clear their guts for ~1 hr prior to respirometry.</p></div>
840626
Krill physiology - In situ conditions
2021-02-11T14:20:21-05:00
2021-02-11T14:20:21-05:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:840626
Physiological observations of Euphausia pacifica sampled in Puget Sound, WA aboard R/V Clifford A. Barnes during cruises CB1073 and CB1078 in 2017.
Physiological observations of Euphausia pacifica sampled in Puget Sound, WA aboard R/V Clifford A. Barnes during cruises CB1073 and CB1078 in 2017.
false
McLaskey, A. K., Keister, J. E. (2021) Physiological observations of Euphausia pacifica sampled in Puget Sound, WA aboard R/V Clifford A. Barnes during cruises CB1073 and CB1078 in 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2) Version Date 2021-03-22 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.840626.2 [access date]
true
2
10.26008/1912/bco-dmo.840626.2
false
Euphausiids
ocean acidification
hypoxia
ETS
AARS
growth
respiration
metabolism
2021-03-22
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840626
http://lod.bco-dmo.org/id/dataset/840626
2017-06-24 - 2017-08-31
2017-06-24
2017-06-24
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