http://lod.bco-dmo.org/id/dataset/840749
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2021-02-15
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Amino acid compound specific isotope analyses of abyssal deposit feeders, gut contents, and surrounding surface sediments collected on R/V Atlantis cruise AT42-10 and R/V Western Flyer Pulse 72 in the eastern North Pacific in 2019
2021-03-30
publication
2021-03-30
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2021-06-03
publication
https://doi.org/10.26008/1912/bco-dmo.840749.1
Jeffrey C. Drazen
University of Hawaiʻi at Mānoa
principalInvestigator
Brian N. Popp
University of Hawaiʻi at Mānoa
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Drazen, J. C., Popp, B. N., Romero, S. (2021) Amino acid compound specific isotope analyses of abyssal deposit feeders, gut contents, and surrounding surface sediments collected on R/V Atlantis cruise AT42-10 and R/V Western Flyer Pulse 72 in the eastern North Pacific in 2019. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-03-30 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.840749.1 [access date]
AA-CSIA of abyssal deposit feeders, their gut contents and the surrounding surface sediments collected on R/V Atlantis cruise AT4210 and R/V Western Flyer cruise Pulse 72 Dataset Description: Methods and Sampling: <p>Sediment cores and megafauna were collected in May and October 2019 using the HOV Alvin and the ROV Doc Ricketts, respectively. Upon retrieval to the surface, samples were placed in a cool room (5º C) for further processing. Sediment cores of 7 centimeters diameter were sliced and the surface sediments (0–0.5 cm) were placed in petri dishes and stored frozen at -80º C. Specimens of megafauna were weighed and measured, then dissected using a scalpel. We made a longitudinal cut along the digestive tract and took a sample of the foregut and hindgut contents, avoiding gut tissue. Then we removed the remaining guts and took a sample from cleaned body tissue, or in the case of echinoids, from the test. All samples were placed in cryovials and frozen in liquid nitrogen, and subsequently stored at -80º C. In the laboratory, samples of sediments, gut contents and body tissue were freeze dried and ground to a homogenous powder using mortar and pestle.<br />
<br />
For analysis of bulk nitrogen and carbon isotopic composition, samples were placed in silver capsules in the following amounts: ~ 0.7 milligrams of body tissue from holothurians, 3 miligrams of body tissue from echinoids, ~ 5 miligrams&nbsp;of gut content, and ~ 20 miligrams of sediment. Samples were acidified to remove carbonates with 1M HCl, which was added dropwise until bubbling ceased, then dried at 60ºC and packed.<br />
<br />
For δ15N and δ13C analysis of individual amino acids, samples were analyzed following the methods of Hannides et al. (2013). For the analysis of carbon isotope composition, we also derivatized and analyzed under the same conditions a sample containing a set of 13 pure amino acids. δ13C values were corrected based on the analysis of that set of pure amino acids (Silfer et al. 1991; Arthur et al. 2014).</p>
<p>&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1829612 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1829612
completed
Jeffrey C. Drazen
University of Hawaiʻi at Mānoa
808-956-6567
Department of Oceanography, SOEST 1000 Pope Road
Honolulu
HI
96822
USA
jdrazen@hawaii.edu
pointOfContact
Brian N. Popp
University of Hawaiʻi at Mānoa
808-956-6206
Department of Earth Sciences 1680 East-West Road
Honolulu
HI
96822
USA
popp@hawaii.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Sample
Cruise
Site
ISO_Date_initial
ISO_Date_final
Latitude
Longitude
Type
Species
Type_gut
delta15N
delta13C
ALA_N
GLY_N
THR_N
SER_N
VAL_N
LEU_N
ILE_N
PRO_N
ASX_N
GLX_N
PHE_N
TYR_N
LYS_N
ALA_N_sd
GLY_N_sd
THR_N_sd
SER_N_sd
VAL_N_sd
LEU_N_sd
ILE_N_sd
PRO_N_sd
ASX_N_sd
GLX_N_sd
PHE_N_sd
TYR_N_sd
LYS_N_sd
ALA_C
GLY_C
THR_C
SER_C
VAL_C
LEU_C
ILE_C
PRO_C
ASX_C
GLX_C
PHE_C
TYR_C
LYS_C
ALA_C_sd
GLY_C_sd
THR_C_sd
SER_C_sd
VAL_C_sd
LEU_C_sd
ILE_C_sd
PRO_C_sd
ASX_C_sd
GLX_C_sd
PHE_C_sd
TYR_C_sd
LYS_C_sd
Costech Model 4010
ConFlo IV (input from IRMS, output to GC)
Thermo Finnigan GC-C III
DeltaPlusXP or Delta-V-Advantage
Thermo Scientific Delta V Plus
Thermo-Fisher Scientific MAT 253
ROV Doc Ricketts
theme
None, User defined
sample identification
Cruise identfier
site
date
latitude
longitude
sample type
species scientific name (binomial)
d15N
d13C
d15N measured in biota
standard deviation
d13C measured in biota
featureType
BCO-DMO Standard Parameters
Alvin tube core
CHN Elemental Analyzer
Continuous Flow Interface for Mass Spectrometers
Gas Chromatograph
Isotope-ratio Mass Spectrometer
Isotope-ratio Mass Spectrometer
Isotope-ratio Mass Spectrometer
Remotely Operated Vehicle
instrument
BCO-DMO Standard Instruments
Pulse 72
AT42-10
Pulse72_ROV_dives
AT42-10_Alvin_dives
service
Deployment Activity
Eastern North Pacific 34.50° N 123.06° W (Station M)
Station M in the eastern North Pacific Ocean
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Assessing the relative importance of small vs large particles as sources of nutrition to abyssal communities
https://osprey.bco-dmo.org/project/775278
Collaborative Research: Assessing the relative importance of small vs large particles as sources of nutrition to abyssal communities
<p><em>NSF Award Abstract:</em><br />
The abyssal plains of the oceans cover roughly half of the earth's surface, host enormous reservoirs of biodiversity and mineral resources, and play important roles in nutrient recycling and carbon sequestration. The most important process controlling the structure and function of these ecosystems is the quantity and quality of food (mostly sinking organic particles) that reaches the deep-sea floor. However, we do not fully understand the processes provisioning this vast ecosystem. We propose to evaluate the relative importance of small and larger "marine snow" particles that sink to deep-sea benthic communities by using the stable isotope signature of amino acids within various food sources and trace their consumption by fauna on the seafloor. This project compares ecosystems from the productive waters off California with the nutrient poor central Pacific, north of Hawaii. This project provides novel insights into how surface ocean processes are coupled to food-webs at the deep ocean seafloor and how changes in food sources potentially impact deep-sea communities. This project also provides excellent training opportunities for graduate students, a postdoctoral researcher, and undergraduates at UH and USC, particularly underrepresented minorities who pursue majors in the geosciences. The project will sponsor an annual G6-12 teacher workshop to inform Hawaii educators about the deep sea and broadly disseminate knowledge to the community. All results are communicated broadly to inform the public as concerns regarding abyssal ecosystems are rising due to interests in deep-sea mining.</p>
<p>The most important process controlling the structure and function of abyssal ecosystems is the quantity and quality of organic material that ultimately reaches the deep-sea floor. Despite the strong relationship between euphotic zone export flux and benthic ecology, studies of abyssal ecosystems have observed a deficit between food supply and benthic community demand. Additional work is therefore needed, particularly with regards to understanding the sources of nutrition to the deep-sea benthos. Recent evidence suggests that small particles may be significant contributors to carbon export, increasing in relative importance with depth in the mesopelagic and reaching the abyssal seafloor. This project is to evaluate the relative importance of small and larger "marine snow" particles to deep-sea benthic communities using a combination of particle flux measurements and state of the art compound specific stable isotope analysis of amino acids (AA-CSIA) at two abyssal locations that contrast in overlying productivity, seasonality, and export magnitude. Time series measurements at these locations (Sta. M off California and Sta. Aloha off Hawaii) provide a rich context for the work. In the mesopelagic central North Pacific larger particles (>53 um) can be resolved from microbially reworked, smaller (0.7-53 um) particles using AA-CSIA. This project is characterizing the isotopic compositions of key individual compounds in a continuum of particle sizes (< 1.0 um suspended particles to large sinking particles >53 um) collected using in situ filtration near the seafloor and bottom-moored sediment traps, thereby defining source-specific isotopic signatures that can be traced into benthic fauna and sediments (that are collected by ROVs and epibenthic sleds). This research to understand pelagic-benthic coupling from particles to megafauna using isotopic measurements at the compound-level will yield novel insights into the importance of small microbially reworked particles to deep-sea benthic food webs. This will more precisely couple surface ocean processes to food-webs at the deep ocean seafloor with implications for understanding climate change effects and the efficiency of energy transfer to higher trophic levels. Furthermore, isotopic measurements can also be used to further parameterize ecosystem models by quantifying trophic position across size classes and thus estimate predator-prey mass ratios in relation to variation in body size spectra, functional type, and ultimately to carbon flux and remineralization. Finally, the results will help refine interpretations of deep-sea paleorecords of past nitrogen dynamics by calibrating potential changes in organic matter isotope values between the surface and seafloor archives.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
Abyssal food web
largerWorkCitation
project
eng; USA
biota
oceans
Eastern North Pacific 34.50° N 123.06° W (Station M); Station M in the eastern North Pacific Ocean
-123.06
-123.06
34.5
34.5
2019-05-01
2019-10-24
California current, Station M (34º 50' N, 123º W) and North Pacific Subtropical Gyre, Station Aloha (22º 45' N, 158º W)
0
BCO-DMO catalogue of parameters from Amino acid compound specific isotope analyses of abyssal deposit feeders, gut contents, and surrounding surface sediments collected on R/V Atlantis cruise AT42-10 and R/V Western Flyer Pulse 72 in the eastern North Pacific in 2019
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/843122.rdf
Name: Sample
Units: unitless
Description: <p>Sample identification code</p>
http://lod.bco-dmo.org/id/dataset-parameter/843123.rdf
Name: Cruise
Units: unitless
Description: <p>Cruise designation</p>
http://lod.bco-dmo.org/id/dataset-parameter/843124.rdf
Name: Site
Units: unitless
Description: <p>Site designation</p>
http://lod.bco-dmo.org/id/dataset-parameter/843125.rdf
Name: ISO_Date_initial
Units: unitless
Description: <p>Initial sampling date UTC (yyyy-mm-dd)</p>
http://lod.bco-dmo.org/id/dataset-parameter/843126.rdf
Name: ISO_Date_final
Units: unitless
Description: <p>Final sampling date UTC (yyyy-mm-dd)</p>
http://lod.bco-dmo.org/id/dataset-parameter/843127.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/843128.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitude (West is negative)</p>
http://lod.bco-dmo.org/id/dataset-parameter/843129.rdf
Name: Type
Units: unitless
Description: <p>Type of sample: Sediment= surface sediment (0-5 mm); Body= deposit feeder body tissue; Gut= gut content</p>
http://lod.bco-dmo.org/id/dataset-parameter/843130.rdf
Name: Species
Units: unitless
Description: <p>Species of deposit feeder: Scotoplanes= <em>S. globosa</em>; Echinocrepis= <em>E. rostrata</em>; Onerophanta= <em>O. mutabilis</em></p>
http://lod.bco-dmo.org/id/dataset-parameter/843131.rdf
Name: Type_gut
Units: unitless
Description: <p>Gut section: Hgut= hindgut; Fgut= foregut</p>
http://lod.bco-dmo.org/id/dataset-parameter/843132.rdf
Name: delta15N
Units: per mil (‰) vs AIR
Description: <p>Bulk nitrogen isotopic composition</p>
http://lod.bco-dmo.org/id/dataset-parameter/843133.rdf
Name: delta13C
Units: per mil (‰) vs VPDB
Description: <p>Bulk carbon isotopic composition</p>
http://lod.bco-dmo.org/id/dataset-parameter/843134.rdf
Name: ALA_N
Units: per mil (‰) vs AIR
Description: <p>Mean delta15N value of all injections for Alanine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843135.rdf
Name: GLY_N
Units: per mil (‰) vs AIR
Description: <p>Mean delta15N value of all injections for Glycine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843136.rdf
Name: THR_N
Units: per mil (‰) vs AIR
Description: <p>Mean delta15N value of all injections for Threonine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843137.rdf
Name: SER_N
Units: per mil (‰) vs AIR
Description: <p>Mean delta15N value of all injections for Serine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843138.rdf
Name: VAL_N
Units: per mil (‰) vs AIR
Description: <p>Mean delta15N value of all injections for Valine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843139.rdf
Name: LEU_N
Units: per mil (‰) vs AIR
Description: <p>Mean delta15N value of all injections for Leucine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843140.rdf
Name: ILE_N
Units: per mil (‰) vs AIR
Description: <p>Mean delta15N value of all injections for Isoleucine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843141.rdf
Name: PRO_N
Units: per mil (‰) vs AIR
Description: <p>Mean delta15N value of all injections for Proline</p>
http://lod.bco-dmo.org/id/dataset-parameter/843142.rdf
Name: ASX_N
Units: per mil (‰) vs AIR
Description: <p>Mean delta15N value of all injections for Aspartic Acid and Asparagine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843143.rdf
Name: GLX_N
Units: per mil (‰) vs AIR
Description: <p>Mean delta15N value of all injections for Glutamic Acid and Glutamine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843144.rdf
Name: PHE_N
Units: per mil (‰) vs AIR
Description: <p>Mean delta15N value of all injections for Phenylalanine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843145.rdf
Name: TYR_N
Units: per mil (‰) vs AIR
Description: <p>Mean delta15N value of all injections for Thyrosine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843146.rdf
Name: LYS_N
Units: per mil (‰) vs AIR
Description: <p>Mean delta15N value of all injections for Lysine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843147.rdf
Name: ALA_N_sd
Units: per mil (‰) vs AIR
Description: <p>Standard deviation of delta15N values from all injections for Alanine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843148.rdf
Name: GLY_N_sd
Units: per mil (‰) vs AIR
Description: <p>Standard deviation of delta15N values from all injections for Glycine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843149.rdf
Name: THR_N_sd
Units: per mil (‰) vs AIR
Description: <p>Standard deviation of delta15N values from all injections for Theronine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843150.rdf
Name: SER_N_sd
Units: per mil (‰) vs AIR
Description: <p>Standard deviation of delta15N values from all injections for Serine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843151.rdf
Name: VAL_N_sd
Units: per mil (‰) vs AIR
Description: <p>Standard deviation of delta15N values from all injections for Valine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843152.rdf
Name: LEU_N_sd
Units: per mil (‰) vs AIR
Description: <p>Standard deviation of delta15N values from all injections for Leucine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843153.rdf
Name: ILE_N_sd
Units: per mil (‰) vs AIR
Description: <p>Standard deviation of delta15N values from all injections for Isoleucine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843154.rdf
Name: PRO_N_sd
Units: per mil (‰) vs AIR
Description: <p>Standard deviation of delta15N values from all injections for Proline</p>
http://lod.bco-dmo.org/id/dataset-parameter/843155.rdf
Name: ASX_N_sd
Units: per mil (‰) vs AIR
Description: <p>Standard deviation of delta15N values from all injections for Aspartic Acid and Asparagine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843156.rdf
Name: GLX_N_sd
Units: per mil (‰) vs AIR
Description: <p>Standard deviation of delta15N values from all injections for Glutamic Acid and Glutamine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843157.rdf
Name: PHE_N_sd
Units: per mil (‰) vs AIR
Description: <p>Standard deviation of delta15N values from all injections for Phenylalanine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843158.rdf
Name: TYR_N_sd
Units: per mil (‰) vs AIR
Description: <p>Standard deviation of delta15N values from all injections for Thyrosine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843159.rdf
Name: LYS_N_sd
Units: per mil (‰) vs AIR
Description: <p>Standard deviation of delta15N values from all injections for Lysine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843160.rdf
Name: ALA_C
Units: per mil (‰) vs VPDB
Description: <p>Mean delta13C value of all injections for Alanine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843161.rdf
Name: GLY_C
Units: per mil (‰) vs VPDB
Description: <p>Mean delta13C value of all injections for Glycine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843162.rdf
Name: THR_C
Units: per mil (‰) vs VPDB
Description: <p>Mean delta13C value of all injections for Theronine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843163.rdf
Name: SER_C
Units: per mil (‰) vs VPDB
Description: <p>Mean delta13C value of all injections for Serine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843164.rdf
Name: VAL_C
Units: per mil (‰) vs VPDB
Description: <p>Mean delta13C value of all injections for Valine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843165.rdf
Name: LEU_C
Units: per mil (‰) vs VPDB
Description: <p>Mean delta13C value of all injections for Leucine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843166.rdf
Name: ILE_C
Units: per mil (‰) vs VPDB
Description: <p>Mean delta13C value of all injections for Isoleucine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843167.rdf
Name: PRO_C
Units: per mil (‰) vs VPDB
Description: <p>Mean delta13C value of all injections for Proline</p>
http://lod.bco-dmo.org/id/dataset-parameter/843168.rdf
Name: ASX_C
Units: per mil (‰) vs VPDB
Description: <p>Mean delta13C value of all injections for Aspartic Acid and Asparagine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843169.rdf
Name: GLX_C
Units: per mil (‰) vs VPDB
Description: <p>Mean delta13C value of all injections for Glutamic Acid and Glutamine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843170.rdf
Name: PHE_C
Units: per mil (‰) vs VPDB
Description: <p>Mean delta13C value of all injections for Phenylalanine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843171.rdf
Name: TYR_C
Units: per mil (‰) vs VPDB
Description: <p>Mean delta13C value of all injections for Thyrosine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843172.rdf
Name: LYS_C
Units: per mil (‰) vs VPDB
Description: <p>Mean delta13C value of all injections for Lysine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843173.rdf
Name: ALA_C_sd
Units: per mil (‰) vs VPDB
Description: <p>Standard deviation of delta13C values from all injections for Alanine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843174.rdf
Name: GLY_C_sd
Units: per mil (‰) vs VPDB
Description: <p>Standard deviation of delta13C values from all injections for Glycine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843175.rdf
Name: THR_C_sd
Units: per mil (‰) vs VPDB
Description: <p>Standard deviation of delta13C values from all injections for Theronine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843176.rdf
Name: SER_C_sd
Units: per mil (‰) vs VPDB
Description: <p>Standard deviation of delta13C values from all injections for Serine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843177.rdf
Name: VAL_C_sd
Units: per mil (‰) vs VPDB
Description: <p>Standard deviation of delta13C values from all injections for Valine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843178.rdf
Name: LEU_C_sd
Units: per mil (‰) vs VPDB
Description: <p>Standard deviation of delta13C values from all injections for Leucine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843179.rdf
Name: ILE_C_sd
Units: per mil (‰) vs VPDB
Description: <p>Standard deviation of delta13C values from all injections for Isoleucine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843180.rdf
Name: PRO_C_sd
Units: per mil (‰) vs VPDB
Description: <p>Standard deviation of delta13C values from all injections for Proline</p>
http://lod.bco-dmo.org/id/dataset-parameter/843181.rdf
Name: ASX_C_sd
Units: per mil (‰) vs VPDB
Description: <p>Standard deviation of delta13C values from all injections for Aspartic Acid and Asparagine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843182.rdf
Name: GLX_C_sd
Units: per mil (‰) vs VPDB
Description: <p>Standard deviation of delta13C values from all injections for Glutamic Acid and Glutamine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843183.rdf
Name: PHE_C_sd
Units: per mil (‰) vs VPDB
Description: <p>Standard deviation of delta13C values from all injections for Phenylalanine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843184.rdf
Name: TYR_C_sd
Units: per mil (‰) vs VPDB
Description: <p>Standard deviation of delta13C values from all injections for Thyrosine</p>
http://lod.bco-dmo.org/id/dataset-parameter/843185.rdf
Name: LYS_C_sd
Units: per mil (‰) vs VPDB
Description: <p>Standard deviation of delta13C values from all injections for Lysine</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
10330
https://darchive.mblwhoilibrary.org/bitstream/1912/27190/1/dataset-840749_abyssal-deposit-feeders-aacsia__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.840749.1
download
onLine
dataset
<p>Sediment cores and megafauna were collected in May and October 2019 using the HOV Alvin and the ROV Doc Ricketts, respectively. Upon retrieval to the surface, samples were placed in a cool room (5º C) for further processing. Sediment cores of 7 centimeters diameter were sliced and the surface sediments (0–0.5 cm) were placed in petri dishes and stored frozen at -80º C. Specimens of megafauna were weighed and measured, then dissected using a scalpel. We made a longitudinal cut along the digestive tract and took a sample of the foregut and hindgut contents, avoiding gut tissue. Then we removed the remaining guts and took a sample from cleaned body tissue, or in the case of echinoids, from the test. All samples were placed in cryovials and frozen in liquid nitrogen, and subsequently stored at -80º C. In the laboratory, samples of sediments, gut contents and body tissue were freeze dried and ground to a homogenous powder using mortar and pestle.<br />
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For analysis of bulk nitrogen and carbon isotopic composition, samples were placed in silver capsules in the following amounts: ~ 0.7 milligrams of body tissue from holothurians, 3 miligrams of body tissue from echinoids, ~ 5 miligrams&nbsp;of gut content, and ~ 20 miligrams of sediment. Samples were acidified to remove carbonates with 1M HCl, which was added dropwise until bubbling ceased, then dried at 60ºC and packed.<br />
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For δ15N and δ13C analysis of individual amino acids, samples were analyzed following the methods of Hannides et al. (2013). For the analysis of carbon isotope composition, we also derivatized and analyzed under the same conditions a sample containing a set of 13 pure amino acids. δ13C values were corrected based on the analysis of that set of pure amino acids (Silfer et al. 1991; Arthur et al. 2014).</p>
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Specified by the Principal Investigator(s)
<p>BCO-DMO processing description:<br />
- Converted dates to ISO date&nbsp;format (yyyy-mm-dd)<br />
- Adjusted field/parameter names to comply with database requirements<br />
- Missing data identifier ‘NA’ replaced with 'nd' (BCO-DMO's default missing data identifier)&nbsp;<br />
- Converted latitudes and longitudes to decimal degrees<br />
- Added a conventional header with dataset name, PI names, version date</p>
<div>&nbsp;</div>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: PI Supplied Instrument Description:Sediment cores and megafauna were collected in May 2019 using the HOV Alvin Instrument Name: Alvin tube core Instrument Short Name:Tube Core Instrument Description: A plastic tube, about 40 cm (16 inches) long, is pushed into the sediment by Alvin's manipulator arm to collect a sediment core.
Costech Model 4010
Costech Model 4010
PI Supplied Instrument Name: Costech Model 4010 PI Supplied Instrument Description:Bulk nitrogen and carbon isotopic composition were determined using an isotope ratio mass spectrometer (DeltaPlusXP or Delta-V-Advantage) coupled to an elemental analyzer (Costech Model 4010).
Instrument Name: CHN Elemental Analyzer Instrument Short Name:CHN_EA Instrument Description: A CHN Elemental Analyzer is used for the determination of carbon, hydrogen, and nitrogen content in organic and other types of materials, including solids, liquids, volatile, and viscous samples.
ConFlo IV (input from IRMS, output to GC)
ConFlo IV (input from IRMS, output to GC)
PI Supplied Instrument Name: ConFlo IV (input from IRMS, output to GC) PI Supplied Instrument Description:Carbon isotope composition of amino acids was measured using a Thermo-Fisher Scientific MAT 253 isotope ratio mass spectrometer interfaced with a Trace Ultra GC-III via ConFlo IV.
Instrument Name: Continuous Flow Interface for Mass Spectrometers Instrument Short Name: Instrument Description: A Continuous Flow Interface connects solid and liquid sample preparation devices to instruments that measure isotopic composition. It allows the introduction of the sample and also reference and carrier gases.
Examples: Finnigan MATConFlo II, ThermoScientific ConFlo IV, and Picarro Caddy.
Note: This is NOT an analyzer
Thermo Finnigan GC-C III
Thermo Finnigan GC-C III
PI Supplied Instrument Name: Thermo Finnigan GC-C III PI Supplied Instrument Description:Nitrogen isotope composition of amino acids was determined using an isotope ratio mass spectrometer (Thermo Scientific Delta V Plus) interfaced with a Thermo Finnigan GC-C III.
Instrument Name: Gas Chromatograph Instrument Short Name:Gas Chromatograph Instrument Description: Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB02/
DeltaPlusXP or Delta-V-Advantage
DeltaPlusXP or Delta-V-Advantage
PI Supplied Instrument Name: DeltaPlusXP or Delta-V-Advantage PI Supplied Instrument Description:Bulk nitrogen and carbon isotopic composition were determined using an isotope ratio mass spectrometer (DeltaPlusXP or Delta-V-Advantage) coupled to an elemental analyzer (Costech Model 4010).
Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Thermo Scientific Delta V Plus
Thermo Scientific Delta V Plus
PI Supplied Instrument Name: Thermo Scientific Delta V Plus PI Supplied Instrument Description:Nitrogen isotope composition of amino acids was determined using an isotope ratio mass spectrometer (Thermo Scientific Delta V Plus) interfaced with a Thermo Finnigan GC-C III. Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Thermo-Fisher Scientific MAT 253
Thermo-Fisher Scientific MAT 253
PI Supplied Instrument Name: Thermo-Fisher Scientific MAT 253 PI Supplied Instrument Description:Carbon isotope composition of amino acids was measured using a Thermo-Fisher Scientific MAT 253 isotope ratio mass spectrometer interfaced with a Trace Ultra GC-III via ConFlo IV.
Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
ROV Doc Ricketts
ROV Doc Ricketts
PI Supplied Instrument Name: ROV Doc Ricketts PI Supplied Instrument Description:Sediment cores and megafauna were collected in October 2019 using the ROV Doc Ricketts Instrument Name: Remotely Operated Vehicle Instrument Short Name:ROV Instrument Description: Remotely operated underwater vehicles (ROVs) are unoccupied, highly maneuverable underwater robots operated by a person aboard a surface vessel. They are linked to the ship by a group of cables that carry electrical signals back and forth between the operator and the vehicle. Most are equipped with at least a video camera and lights. Additional equipment is commonly added to expand the vehicle’s capabilities. These may include a still camera, a manipulator or cutting arm, water samplers, and instruments that measure water clarity, light penetration, and temperature.
Cruise: Pulse 72
Pulse 72
R/V Western Flyer
International Council for the Exploration of the Sea
R/V Western Flyer
vessel
Pulse 72
Kenneth Smith
Monterey Bay Aquarium Research Institute
Cruise: AT42-10
AT42-10
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
AT42-10
Jeffrey C. Drazen
University of Hawaiʻi at Mānoa
Deployment: Pulse72_ROV_dives
Pulse72_ROV_dives
Doc Ricketts
International Council for the Exploration of the Sea
Doc Ricketts
ROV
Deployment: AT42-10_Alvin_dives
AT42-10_Alvin_dives
HOV Alvin
Community Standard Description
International Council for the Exploration of the Sea
HOV Alvin
submarine-manned
R/V Western Flyer
International Council for the Exploration of the Sea
R/V Western Flyer
vessel
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
Doc Ricketts
International Council for the Exploration of the Sea
Doc Ricketts
ROV
HOV Alvin
Community Standard Description
International Council for the Exploration of the Sea
HOV Alvin
submarine-manned