http://lod.bco-dmo.org/id/dataset/840875
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2021-02-17
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Concentrations of mercury forms and ancillary parameters in size fractionated plankton samples and in water collected during 2014 from Long Island Sound and the adjacent shelf
2021-06-07
publication
2021-06-07
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2021-06-11
publication
https://doi.org/10.26008/1912/bco-dmo.840875.1
Robert P. Mason
University of Connecticut
principalInvestigator
Kathleen J. Gosnell
University of Connecticut
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Mason, R. P., Gosnell, K. J. (2021) Concentrations of mercury forms and ancillary parameters in size fractionated plankton samples and in water collected during 2014 from Long Island Sound and the adjacent shelf. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-06-07 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.840875.1 [access date]
Methods and Sampling: <p><strong>Sampling and analytical procedures:</strong><br />
Methods are described in detail in Gosnell et al. (2017).&nbsp;</p>
<p>Samples were collected to represent three separate seasons during 2014:&nbsp; Spring (May 20th-22nd), Summer (August 2nd-5th), and Fall (September 9th-11th).&nbsp; Plankton and water samples were collected from Western Long Island Sound (WLIS) and Eastern Long Island Sound (ELIS) stations in the spring and summer, while the fall collection included stations in Central Long Island Sound (CLIS), as well as along the shelf break (SB) and mid-shelf (MS) region.&nbsp;</p>
<p>At each station, triplicate substations were sampled approximately one mile apart at each site during the spring and summer seasons, while duplicate substations were sampled during the fall. Water samples were collected using a trace-metal clean GoFlo bottle attached to a Kevlar line, or with a trace-metal clean rosette system. Water was kept cold and dark until processing.&nbsp; Water samples were filtered for particulate methylmercury (MeHg) and total mercury (HgT), total suspended solids (TSS) and chlorophyll a (Chl a). Filtrate was saved for dissolved mercury and methylmercury, nutrients, and dissolved organic carbon (DOC) measurements. A CTD was deployed concurrent with water collections, and recorded the vertical profiles of fluorescence, oxygen, temperature, and salinity. Separate phytoplankton (seston) fractions were obtained by sequentially filtering the seawater through polycarbonate filters of sizes 0.2 µm, 5&nbsp;µm&nbsp;and 20 µm. Larger particulate material was excluded by initially passing water through a 200 µm mesh. Zooplankton were collected by attaching an opening/closing 200 µm net (Seatec) to the Kevlar line. Deployments were from 2 meters above the bottom up to 1 meter from the surface. Depths ranged from 11 meter in Long Island Sound, to 100 meter at the Mid-Shelf&nbsp;and Shelf Break stations. Zooplankton were separated into size fractions of 0.2–0.5 millimeters, 0.5–1 mm, 1–2 mm, and&nbsp; &gt;2 mm&nbsp;via mesh screens.</p>
<p>Each sample was digested in 4.5 M HNO3 acid solution at 60 degrees C for at least 12 hours prior to analysis. For MeHg analysis, a subsample was neutralized with KOH, diluted to a final volume of 30 mililiters, buffered with acetate buffer (pH of 4.9), and ethylated with sodium tetraethylborate (Hammerschmidt et al. 2013). Water samples were analyzed without acid digestion (Munson et al., 2014). Samples were then analyzed using a Tekran 2700 methylmercury analyzer with cold vapor atomic fluorescence (CVAFS) detection, and an external calibration curve (r2 &gt;0.998). For total Hg analysis (HgT), the remaining digest was diluted, bromine monochloride (BrCl) was added for a minimum 24 hours preceding analysis to decompose organic matter and convert all Hg into the ionic form. Hydroxylamine hydrochloride was added to degrade excess BrCl oxidant prior to analysis using a manual system with tin chloride (SnCl2) reduction, purging with N2 onto a trap containing gold-coated beads for quantification using a Tekran 2500 CVAFS detector, and an external calibration curve (r2 &gt;0.999). For water samples, the acidified water was treated with BrCl and then processed as for biota digests. Chlorophyll and phaeopigment were analyzed using a Trilogy fluorometer, and DOC was analyzed using a Shimazdu TOC/TN instrument.</p>
<p><strong>Additional information:</strong><br />
QA/QC information is given in Table 1. (see Supplemental Files section below)&nbsp;<br />
<br />
Results of these data are shown in Figs 2-5 and Table 1 in Gosnell et al. (2017). Additional calculated information (%MeHg, bioconcentration factors) is included in the same paper in additional tables. Stable isotope data for C, N and S for zooplankton is also reported.</p>
<p><strong>Problem report:</strong><br />
Not all size fractions of samples were collected at all locations because of biota variability. Missing information is indicated in the dataset as 'nd'. Additionally,&nbsp;Spring and Summer samples were collected only at two sites with additional Fall&nbsp;sampling at three other sites.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1634048 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1634048
completed
Robert P. Mason
University of Connecticut
860-405-9129
Department of Marine Sciences
Groton
CT
06340
USA
Robert.mason@uconn.edu
pointOfContact
Kathleen J. Gosnell
University of Connecticut
kgosnell@geomar.de
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Season
Location
Location_name
Latitude
Longitude
DateTime_Local_EDT
Sample_type_size
Sample_size_id
Sample_size
Num_samples
MeHg
MeHg_stdev
HgT
HgT_stdev
Depth
Depth_description
Diss_HgT
Diss_MeHg
Part_HgT
Part_MeHg
TSS
DOC
Chl_a
ISO_DateTime_UTC
Tekran 2700 Automated Methylmercury Analysis System
Trilogy fluorometer
Tekran 2500 Total Mercury Analysis System
Shimadzu TOC/TN analyzer
theme
None, User defined
season
site description
site
latitude
longitude
date
sample type
sample description
number
methylmercury
standard deviation
total mercury
depth
suspended particulate matter concentration
dissolved organic Carbon
chlorophyll a
Date and time
featureType
BCO-DMO Standard Parameters
Automated Mercury Analysis System
Fluorometer
Tekran 2500 CVAFS mercury detector
Total Organic Carbon Analyzer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Transformations and mercury isotopic fractionation of methylmercury by marine phytoplankton
https://osprey.bco-dmo.org/project/810499
Collaborative Research: Transformations and mercury isotopic fractionation of methylmercury by marine phytoplankton
<p><em>NSF Award Abstract:</em><br />
The accumulation of mercury (Hg) in seafood is a public health concern. The presence of Hg in seafood depends to a large degree on the air-sea exchange of Hg, with atmospheric deposition leading to accumulation of Hg in the ocean. The pathways to seafood start with the uptake of Hg by phytoplankton from seawater where is has always been assumed to accumulate to be eaten by grazers and passed on to larger organisms. This project challenges this assumption with preliminary data that suggests certain phytoplankton species can transform Hg to volatile forms (mercury vapor & dimethylmercury) that are lost to the atmosphere, a processes that removes Hg from the ocean rather than simply concentrating it into the ecosystem and seafood. This process, which has not been studied before, could dramatically alter our view of the Hg cycle in the ocean. The researchers funded by this project will look for the specific phytoplankton species that are capable of volatilizing Hg and quantify the rates at which they do so. They will also examine the suspected role of associated sulfur and selenium compounds in the process, as well as quantifying the changes in the Hg isotopic values for potential use as chemical tracers of the source of Hg in the ecosystem and food supply. These results should allow oceanographers to better quantify and refine our knowledge of Hg cycling in the ocean. The project will support participation of graduate students, a postdoctoral scientist, and incorporation of new information directly into courses taught by the researchers. Funding will also support continuing activities by the participants in activities that disseminate information on mercury and its effect on public and environmental health.</p>
<p>Biogeochemical cycling of mercury (Hg) in the ocean may be more complex than previously assumed. New evidence has challenged the idea that methylmercury (MeHg) merely accumulates in phytoplankton and undergoes little to no transformation before being passed into the food web. This project aims to more fully elucidate the mechanisms behind the intracellular transformation of MeHg to volatile Hg and dimethylmercury (Me2Hg) that can be lost to the atmosphere, as well as to evaluate the range of algal taxa that can perform this transformation using directed culture work. Additionally, the PIs will investigate evidence that thiols, organic selenium (Se) compounds, and sulfides are required to facilitate these reactions within the phytoplankton, and specific pathways will be investigated and quantified through this research. Stable Hg isotopic data has been used to track Hg sources and pathways in marine systems and its fractionation during these MeHg transformations will also be quantified for future field study of marine Hg. The investigators hypothesize that coccolithophorids and other haptophytes capable of these intracellular reactions may account for a significant portion of the production of volatile Hg in the ocean. If this turns out to be the case, understanding and quantifying these volatilization processes may significantly alter our current understanding of the overall biogeochemical cycling of Hg in the ocean.</p>
Phytoplankton MeHg
largerWorkCitation
project
eng; USA
biota
oceans
-73.56
-70.956
40.228
41.263
2014-05-20
2014-09-11
Antarctic Peninsula
0
BCO-DMO catalogue of parameters from Concentrations of mercury forms and ancillary parameters in size fractionated plankton samples and in water collected during 2014 from Long Island Sound and the adjacent shelf
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/853158.rdf
Name: Season
Units: unitless
Description: <p>Season of sample collection (Spring, Summer, Fall)</p>
http://lod.bco-dmo.org/id/dataset-parameter/853159.rdf
Name: Location
Units: unitless
Description: <p>Location in Long Island Sound or on the associated shelf</p>
http://lod.bco-dmo.org/id/dataset-parameter/853160.rdf
Name: Location_name
Units: unitless
Description: <p>Western (WLIS), Central (CLIS), or Eastern (ELIS) Long Island Sounds; Mid-Shelf (MS); and Shelf Break (SB)</p>
http://lod.bco-dmo.org/id/dataset-parameter/853161.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitude of sampling</p>
http://lod.bco-dmo.org/id/dataset-parameter/853162.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitude of sampling</p>
http://lod.bco-dmo.org/id/dataset-parameter/853163.rdf
Name: DateTime_Local_EDT
Units: unitless
Description: <p>DateTime of sampling (yyyy-mm-dd hh:mm:ss) in Eastern time zone</p>
http://lod.bco-dmo.org/id/dataset-parameter/853166.rdf
Name: Sample_type_size
Units: unitless
Description: <p>Sample type (Zooplankton or Phytoplankton)</p>
http://lod.bco-dmo.org/id/dataset-parameter/853167.rdf
Name: Sample_size_id
Units: unitless
Description: <p>Size description of the sample (small, medium, large, x-large)</p>
http://lod.bco-dmo.org/id/dataset-parameter/853168.rdf
Name: Sample_size
Units: unitless
Description: <p>Size (in microns or millimeters) of the sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/853169.rdf
Name: Num_samples
Units: each
Description: <p>Number of samples collected and used for calculating standard deviation</p>
http://lod.bco-dmo.org/id/dataset-parameter/853170.rdf
Name: MeHg
Units: picomol per gram (wet weight)
Description: <p>Average methylmercury (MeHg) concentration in each seston size fraction on a wet weight basis</p>
http://lod.bco-dmo.org/id/dataset-parameter/853171.rdf
Name: MeHg_stdev
Units: picomol per gram (wet weight)
Description: <p>Standard deviation for the MeHg concentration for the size fraction. (If <2 samples, no standard deviation is given)</p>
http://lod.bco-dmo.org/id/dataset-parameter/853172.rdf
Name: HgT
Units: picomol per gram (wet weight)
Description: <p>Average total mercury (HgT) concentration in each seston size fraction on a wet weight basis</p>
http://lod.bco-dmo.org/id/dataset-parameter/853173.rdf
Name: HgT_stdev
Units: picomol per gram (wet weight)
Description: <p>Standard deviation for the HgT concentration. (If <2 samples, no standard deviation is given)</p>
http://lod.bco-dmo.org/id/dataset-parameter/853174.rdf
Name: Depth
Units: meters (m)
Description: <p>Water depth for the water collections (mostly 2 per location)</p>
http://lod.bco-dmo.org/id/dataset-parameter/853175.rdf
Name: Depth_description
Units: units
Description: <p>Water column location</p>
http://lod.bco-dmo.org/id/dataset-parameter/853176.rdf
Name: Diss_HgT
Units: picomol per liter
Description: <p>Dissolved total mercury (<0.2 µm)</p>
http://lod.bco-dmo.org/id/dataset-parameter/853177.rdf
Name: Diss_MeHg
Units: picomol per liter
Description: <p>Dissolved methylmercury (<0.2 µm)</p>
http://lod.bco-dmo.org/id/dataset-parameter/853178.rdf
Name: Part_HgT
Units: picomol per gram (dry weight)
Description: <p>Particulate total mercury on a dry weight basis</p>
http://lod.bco-dmo.org/id/dataset-parameter/853179.rdf
Name: Part_MeHg
Units: picomol per gram (dry weight)
Description: <p>Particulate methylmercury on a dry weight basis</p>
http://lod.bco-dmo.org/id/dataset-parameter/853180.rdf
Name: TSS
Units: milligram per liter (dry weight)
Description: <p>Total suspended solids</p>
http://lod.bco-dmo.org/id/dataset-parameter/853181.rdf
Name: DOC
Units: micomol per liter (µM)
Description: <p>Dissolved organic carbon (<0.2 µm)</p>
http://lod.bco-dmo.org/id/dataset-parameter/853182.rdf
Name: Chl_a
Units: microgram per liter (µg/L)
Description: <p>Chlorophyll a concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/853317.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>DateTime of sampling in UTC/GMT</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
13787
https://darchive.mblwhoilibrary.org/bitstream/1912/27228/1/dataset-840875_lis-plankton__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.840875.1
download
onLine
dataset
<p><strong>Sampling and analytical procedures:</strong><br />
Methods are described in detail in Gosnell et al. (2017).&nbsp;</p>
<p>Samples were collected to represent three separate seasons during 2014:&nbsp; Spring (May 20th-22nd), Summer (August 2nd-5th), and Fall (September 9th-11th).&nbsp; Plankton and water samples were collected from Western Long Island Sound (WLIS) and Eastern Long Island Sound (ELIS) stations in the spring and summer, while the fall collection included stations in Central Long Island Sound (CLIS), as well as along the shelf break (SB) and mid-shelf (MS) region.&nbsp;</p>
<p>At each station, triplicate substations were sampled approximately one mile apart at each site during the spring and summer seasons, while duplicate substations were sampled during the fall. Water samples were collected using a trace-metal clean GoFlo bottle attached to a Kevlar line, or with a trace-metal clean rosette system. Water was kept cold and dark until processing.&nbsp; Water samples were filtered for particulate methylmercury (MeHg) and total mercury (HgT), total suspended solids (TSS) and chlorophyll a (Chl a). Filtrate was saved for dissolved mercury and methylmercury, nutrients, and dissolved organic carbon (DOC) measurements. A CTD was deployed concurrent with water collections, and recorded the vertical profiles of fluorescence, oxygen, temperature, and salinity. Separate phytoplankton (seston) fractions were obtained by sequentially filtering the seawater through polycarbonate filters of sizes 0.2 µm, 5&nbsp;µm&nbsp;and 20 µm. Larger particulate material was excluded by initially passing water through a 200 µm mesh. Zooplankton were collected by attaching an opening/closing 200 µm net (Seatec) to the Kevlar line. Deployments were from 2 meters above the bottom up to 1 meter from the surface. Depths ranged from 11 meter in Long Island Sound, to 100 meter at the Mid-Shelf&nbsp;and Shelf Break stations. Zooplankton were separated into size fractions of 0.2–0.5 millimeters, 0.5–1 mm, 1–2 mm, and&nbsp; &gt;2 mm&nbsp;via mesh screens.</p>
<p>Each sample was digested in 4.5 M HNO3 acid solution at 60 degrees C for at least 12 hours prior to analysis. For MeHg analysis, a subsample was neutralized with KOH, diluted to a final volume of 30 mililiters, buffered with acetate buffer (pH of 4.9), and ethylated with sodium tetraethylborate (Hammerschmidt et al. 2013). Water samples were analyzed without acid digestion (Munson et al., 2014). Samples were then analyzed using a Tekran 2700 methylmercury analyzer with cold vapor atomic fluorescence (CVAFS) detection, and an external calibration curve (r2 &gt;0.998). For total Hg analysis (HgT), the remaining digest was diluted, bromine monochloride (BrCl) was added for a minimum 24 hours preceding analysis to decompose organic matter and convert all Hg into the ionic form. Hydroxylamine hydrochloride was added to degrade excess BrCl oxidant prior to analysis using a manual system with tin chloride (SnCl2) reduction, purging with N2 onto a trap containing gold-coated beads for quantification using a Tekran 2500 CVAFS detector, and an external calibration curve (r2 &gt;0.999). For water samples, the acidified water was treated with BrCl and then processed as for biota digests. Chlorophyll and phaeopigment were analyzed using a Trilogy fluorometer, and DOC was analyzed using a Shimazdu TOC/TN instrument.</p>
<p><strong>Additional information:</strong><br />
QA/QC information is given in Table 1. (see Supplemental Files section below)&nbsp;<br />
<br />
Results of these data are shown in Figs 2-5 and Table 1 in Gosnell et al. (2017). Additional calculated information (%MeHg, bioconcentration factors) is included in the same paper in additional tables. Stable isotope data for C, N and S for zooplankton is also reported.</p>
<p><strong>Problem report:</strong><br />
Not all size fractions of samples were collected at all locations because of biota variability. Missing information is indicated in the dataset as 'nd'. Additionally,&nbsp;Spring and Summer samples were collected only at two sites with additional Fall&nbsp;sampling at three other sites.</p>
Specified by the Principal Investigator(s)
<p>Data was processed using spreadsheets in Excel and no other post-analytical data processing occurred</p>
<p>BCO-DMO Processing:<br />
- data was rearranged to list both Zooplankton and Phytoplankton species by season, location, and size<br />
- values rounded to four decimal places for MeHg and HgT columns<br />
- added&nbsp;fields for sample type, size ID, and description of depth<br />
- replaced asterisk in standard deviation with 'nd' and created a column to list the number of samples&nbsp;<br />
- dates and times joined to main data set</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Tekran 2700 Automated Methylmercury Analysis System
Tekran 2700 Automated Methylmercury Analysis System
PI Supplied Instrument Name: Tekran 2700 Automated Methylmercury Analysis System PI Supplied Instrument Description:Tekran 2700 Automated Methylmercury Analysis System (which incorporates gas chromatography and cold vapor atomic fluorescence detection)
Instrument Name: Automated Mercury Analysis System Instrument Short Name: Instrument Description: Examples include Tekran Models 2600 and 2700
Trilogy fluorometer
Trilogy fluorometer
PI Supplied Instrument Name: Trilogy fluorometer PI Supplied Instrument Description:Chlorophyll and phaeopigment were analyzed using a Trilogy fluorometer Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/
Tekran 2500 Total Mercury Analysis System
Tekran 2500 Total Mercury Analysis System
PI Supplied Instrument Name: Tekran 2500 Total Mercury Analysis System PI Supplied Instrument Description:Tekran 2500 Total Mercury Analysis System (not automated; cold vapor atomic fluorescence spectrometry) Instrument Name: Tekran 2500 CVAFS mercury detector Instrument Short Name: Instrument Description: Tekran 2500 Total Mercury Analysis System (not automated; cold vapor atomic fluorescence spectrometry)
Shimadzu TOC/TN analyzer
Shimadzu TOC/TN analyzer
PI Supplied Instrument Name: Shimadzu TOC/TN analyzer PI Supplied Instrument Description:DOC was analyzed on a Shimadzu TOC/TN analyzer. Instrument Name: Total Organic Carbon Analyzer Instrument Short Name:TOC analyzer Instrument Description: A unit that accurately determines the carbon concentrations of organic compounds typically by detecting and measuring its combustion product (CO2). See description document at: http://bcodata.whoi.edu/LaurentianGreatLakes_Chemistry/bs116.pdf Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/