http://lod.bco-dmo.org/id/dataset/842922 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2021-03-02 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Seawater conditions monitored and recorded during two separate laboratory experiments in 2017 to acclimate krill to dissolved oxygen (DO) or pH conditions. 2021-03-02 publication 2021-03-02 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2021-03-24 publication https://doi.org/10.26008/1912/bco-dmo.842922.1 Julie E. Keister University of Washington principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: McLaskey, A. K., Keister, J. E. (2021) Seawater conditions monitored and recorded during two separate laboratory experiments in 2017 to acclimate krill to dissolved oxygen (DO) or pH conditions. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-03-02 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.842922.1 [access date] Laboratory krill experiments Dataset Description: Methods and Sampling: &lt;p&gt;Two separate experiments were conducted to test the effects of acclimation to different pH or dissolved oxygen (DO) conditions on adult female &lt;em&gt;Euphausia pacifica. &lt;/em&gt;System indicates the system that generated controlled seawater conditions with many individual krill held within one experimental system. Responses measured after the ten day acclimation (ETS activity, AARS activity, and respiration rate) are given in the Krill Physiology dataset along with the corresponding system name.&lt;/p&gt; &lt;p&gt;Acclimation of krill to different oxygen levels in the laboratory was done at the NOAA Mukilteo Research Station in flow-through experimental systems with independent temperature, pH, and oxygen control. Nine systems of generating controlled seawater were used, three per treatment. Target dissolved oxygen levels were 3, 5, and 9 mg DO L&lt;sup&gt;-1&lt;/sup&gt;; temperature was maintained at 12 °C and pH at ~7.82. During the oxygen experiment, temperature, pH, and dissolved oxygen were monitored continuously using Omega thermistors, Honeywell Durafet III probes, and Vernier optical dissolved oxygen probes, respectively. Temperature, pH, and oxygen concentration were recorded in each system every 6 seconds, but were binned into 10 min averages.&lt;/p&gt; &lt;p&gt;Experimental acclimation to pH was conducted at the FHL Ocean Acidification Environmental Laboratory, using a flow-through system with pH controlled by CO2 bubbling. Target pH levels were 8.0, 7.5, and 7.2: three flow-through systems were used, one per pH treatment. Temperature was set to 12 °C and oxygen was maintained at ambient levels through bubbling, but not monitored. Temperature and pH were monitored and logged every 10 min with a Honeywell Durafet III electrode. Temperature failed to record in system 104A but this system shared a cooling unit with system 104B and was therefore likely similar.&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1154648 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1154648 completed Julie E. Keister University of Washington 206-543-7620 School of Oceanography Box 357940 Seattle WA 98195 United States jkeister@uw.edu pointOfContact asNeeded Dataset Version: 1 Unknown Experiment ISO_DateTime_UTC System Temperature pH DO Vernier optical dissolved oxygen probes Honeywell Durafet III pH probes Omega thermistors theme None, User defined sample description ISO_DateTime_UTC water temperature at measurement depth pH dissolved Oxygen featureType BCO-DMO Standard Parameters Oxygen Sensor pH Sensor Thermistor instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Consequences of hypoxia on food web linkages in a pelagic marine ecosystem https://osprey.bco-dmo.org/project/557504 Consequences of hypoxia on food web linkages in a pelagic marine ecosystem <p><em>Description from NSF award abstract:</em><br /> Low dissolved oxygen (hypoxia) is one of the most pronounced, pervasive, and significant disturbances in marine ecosystems. Yet, our understanding of the ecological impacts of hypoxia on pelagic food webs is incomplete because of our limited knowledge of how organism responses to hypoxia affect critical ecosystem processes. In pelagic food webs, distribution shifts of mesozooplankton and their predators may affect predator-prey overlap and dictate energy flow up food webs. Similarly, hypoxia may induce shifts in zooplankton community composition towards species that impede energy flow to planktivorous fish. However, compensatory responses by species and communities might negate these effects, maintaining trophic coupling and sustaining productivity of upper trophic level species. The PIs propose to answer the question "Does hypoxia affect energy flow from mesozooplankton to pelagic fish?" They approach this question with a nested framework of hypotheses that considers two sets of processes alternatively responsible for either changes or maintenance of pelagic ecosystem energy flows. They will conduct their study in the Hood Canal, WA. Unlike most hypoxia-impacted estuaries, hypoxic regions of Hood Canal are in close proximity to sites that are not affected. This makes it logistically easier to conduct a comparative study and reduces the number of potential confounding factors when comparing areas that are far apart.</p> <p>Improved understanding of how hypoxia impacts marine ecosystems will benefit the practical application of ecosystem-based management (EBM) in coastal and estuarine ecosystems. Effective application of EBM requires that the impacts of human activities are well understood and that ecological effects can be tracked using indicators. This project will contribute to both of these needs. The PIs will share their findings on local and national levels with Federal, State, Tribal, and County biologists. To increase exposure of science to underrepresented groups, the PIs also will provide Native American youth with opportunities to participate in field collections and laboratory processing through summer internships. The PIs will collaborate with the NSF-funded Pacific Northwest Louis Stokes Alliance for Minority Participation and tribes from the Hood Canal region to recruit and mentor students for potential careers in marine science. This project will support several undergraduate researchers, two Ph.D. students, a post-doc, and two early-career scientists.</p> PelagicHypoxia largerWorkCitation project eng; USA oceans 2017-07-24 2017-10-02 Puget Sound, WA (47 N, 123 W) 0 BCO-DMO catalogue of parameters from Seawater conditions monitored and recorded during two separate laboratory experiments in 2017 to acclimate krill to dissolved oxygen (DO) or pH conditions. Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/842932.rdf Name: Experiment Units: unitless Description: <p>Experiment type: pH or DO experiment</p> http://lod.bco-dmo.org/id/dataset-parameter/842933.rdf Name: ISO_DateTime_UTC Units: unitless Description: <p>Sampling date and time in ISO format (yyyy-mm-ddThh:mm), UTC timezone</p> http://lod.bco-dmo.org/id/dataset-parameter/842934.rdf Name: System Units: units Description: <p>The system that generated controlled seawater conditions and represents the experimental replicates. Many individual krill were held within one experimental system. For the pH experiment 3 systems were used, only one per treatment condition (104A, 104B, 105A); for the DO experiment nine systems were used with three per treatment condition (M1, M2, M3, M4, M5, M9, M10, M11, M12)</p> http://lod.bco-dmo.org/id/dataset-parameter/842935.rdf Name: Temperature Units: degrees Celsius (°C) Description: <p>water temperature</p> http://lod.bco-dmo.org/id/dataset-parameter/842936.rdf Name: pH Units: unitless Description: <p>pH on the total scale</p> http://lod.bco-dmo.org/id/dataset-parameter/842937.rdf Name: DO Units: milligrams per liter (mg/L) Description: <p>Dissolved oxygen</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2291214 https://darchive.mblwhoilibrary.org/bitstream/1912/26862/1/dataset-842922_krill-experiment-experimental-conditions__v1.tsv download https://doi.org/10.26008/1912/bco-dmo.842922.1 download onLine dataset &lt;p&gt;Two separate experiments were conducted to test the effects of acclimation to different pH or dissolved oxygen (DO) conditions on adult female &lt;em&gt;Euphausia pacifica. &lt;/em&gt;System indicates the system that generated controlled seawater conditions with many individual krill held within one experimental system. Responses measured after the ten day acclimation (ETS activity, AARS activity, and respiration rate) are given in the Krill Physiology dataset along with the corresponding system name.&lt;/p&gt; &lt;p&gt;Acclimation of krill to different oxygen levels in the laboratory was done at the NOAA Mukilteo Research Station in flow-through experimental systems with independent temperature, pH, and oxygen control. Nine systems of generating controlled seawater were used, three per treatment. Target dissolved oxygen levels were 3, 5, and 9 mg DO L&lt;sup&gt;-1&lt;/sup&gt;; temperature was maintained at 12 °C and pH at ~7.82. During the oxygen experiment, temperature, pH, and dissolved oxygen were monitored continuously using Omega thermistors, Honeywell Durafet III probes, and Vernier optical dissolved oxygen probes, respectively. Temperature, pH, and oxygen concentration were recorded in each system every 6 seconds, but were binned into 10 min averages.&lt;/p&gt; &lt;p&gt;Experimental acclimation to pH was conducted at the FHL Ocean Acidification Environmental Laboratory, using a flow-through system with pH controlled by CO2 bubbling. Target pH levels were 8.0, 7.5, and 7.2: three flow-through systems were used, one per pH treatment. Temperature was set to 12 °C and oxygen was maintained at ambient levels through bubbling, but not monitored. Temperature and pH were monitored and logged every 10 min with a Honeywell Durafet III electrode. Temperature failed to record in system 104A but this system shared a cooling unit with system 104B and was therefore likely similar.&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;BCO-DMO processing notes:&lt;/p&gt; &lt;ul&gt; &lt;li&gt;Converted pacific daylight date and time to ISO_DateTime, in UTC timezone.&lt;/li&gt; &lt;li&gt;Adjusted parameter names to comply with database requirements.&lt;/li&gt; &lt;/ul&gt; Specified by the Principal Investigator(s) asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact Vernier optical dissolved oxygen probes Vernier optical dissolved oxygen probes PI Supplied Instrument Name: Vernier optical dissolved oxygen probes PI Supplied Instrument Description:Vernier optical dissolved oxygen probes Instrument Name: Oxygen Sensor Instrument Short Name:Dissolved Oxygen Sensor Instrument Description: An electronic device that measures the proportion of oxygen (O2) in the gas or liquid being analyzed Honeywell Durafet III pH probes Honeywell Durafet III pH probes PI Supplied Instrument Name: Honeywell Durafet III pH probes PI Supplied Instrument Description:Honeywell Durafet III pH probes Instrument Name: pH Sensor Instrument Short Name:pH Sensor Instrument Description: An instrument that measures the hydrogen ion activity in solutions. The overall concentration of hydrogen ions is inversely related to its pH.  The pH scale ranges from 0 to 14 and indicates whether acidic (more H+) or basic (less H+).  Omega thermistors Omega thermistors PI Supplied Instrument Name: Omega thermistors Instrument Name: Thermistor Instrument Short Name: Instrument Description: A thermistor is a type of resistor whose resistance varies significantly with temperature, more so than in standard resistors. The word is a portmanteau of thermal and resistor. Thermistors are widely used as inrush current limiters, temperature sensors, self-resetting overcurrent protectors, and self-regulating heating elements. Thermistors differ from resistance temperature detectors (RTD) in that the material used in a thermistor is generally a ceramic or polymer, while RTDs use pure metals. The temperature response is also different; RTDs are useful over larger temperature ranges, while thermistors typically achieve a higher precision within a limited temperature range, typically 90C to 130C.