http://lod.bco-dmo.org/id/dataset/844810
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2021-03-11
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Carbon and nitrogen stable isotopes of marine inorganic and organic matter around O'ahu, Hawai'i, December 2017
2021-03-11
publication
2021-03-11
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2021-03-12
publication
https://doi.org/10.26008/1912/bco-dmo.844810.1
Andréa G. Grottoli
Ohio State University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Grottoli, A. G. (2021) Carbon and nitrogen stable isotopes of marine inorganic and organic matter around O'ahu, Hawai'i, December 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-03-11 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.844810.1 [access date]
CN isotopes inorganic/organic matter Hawai'i Dataset Description: Methods and Sampling: <p>Dissolved inorganic carbon (DIC) – 20 ml of seawater was filtered (0.45 µm pore size) and preserved in a glass crimp-top bottle with 200 µl of dried mercuric chloride for subsequent isotope analysis. The remaining seawater collected from each site was pre-filtered through 55 µm nylon mesh to remove large particulates and zooplankton. The d13C of DIC samples was analyzed at the Duke University Environmental Isotope Laboratory using a Thermo Finnigan Delta Plus XL continuous flow mass spectrometer via a ThermoFinnigan GasBench II and reported in permil relative to V-PDB. Repeated measurements of internal standards had a standard deviation of ± 0.2 per mil.</p>
<p>Dissolved organic carbon (DOC) – 40 ml of seawater was filtered through a quartz microfiber filter into a polytetrafluoroethylene bottle and acidified using 10% HCl to reach a pH below 2 to preserve the sample. The d13C of DOC samples was analyzed using an OI Analytical 1030D Total Organic Carbon Analyzer in the Aquatic Biogeochemical Laboratory at North Carolina State University and reported in permil relative to V-PDB. Repeated measurements of external standards had a standard deviation of ± 0.4 per mil.</p>
<p>Particulate organic matter (POM) – Between 3 – 4 L of the seawater was filtered through a quartz microfiber&nbsp; filter until the filter became light brown in color and was immediately stored at –20 degrees C for subsequent isotopic analysis of particulate organic carbon (POC) and particulate organic nitrogen (PON). The POM filters were fumigated with 12 M hydrochloric acid to remove carbonates (Moyer et al. 2013). All samples were combusted using a PDZ Europa ANCA-GSL elemental analyzer interfaced to a PDZ Europa 20-20 isotope ratio mass spectrometer (Sercon Ltd., Cheshire, UK) at the University of California (UC) Davis Stable Isotope Facility. The d13C and d15N values are reported in permil relative to V-PDB and air, respectively. The standard deviation of replicate analyses for these POM samples was ± 0.31 per mil&nbsp;for d13C. The standard deviations of replicate analyses for d15N of POM was not able to be calculated due lack of additional sample material.</p>
<p>Zooplankton – Zooplankton were collected using a bucket with an illuminated dive torch affixed to the bottom, which was placed on the seafloor near the reef at 1 m depth for 5 min. The zooplankton were separated into 150 – 400 µm and 400 – 800 µm size fractions in the field, stored on ice, and then isolated onto a glass fiber filter and stored at –20 degrees&nbsp;C upon return to the lab the same day. The zooplankton samples were processed separately for d13C and d15N. For d13C, zooplankton samples were fumigated with 12 M hydrochloric acid to remove carbonates. The samples were processed for d15N without pretreatment to ensure that these isotope values were unaffected by acidification. All samples were combusted using a PDZ Europa ANCA-GSL elemental analyzer interfaced to a PDZ Europa 20-20 isotope ratio mass spectrometer (Sercon Ltd., Cheshire, UK) at the University of California (UC) Davis Stable Isotope Facility. The d13C and d15N values are reported in permil relative to V-PDB and air, respectively. The standard deviation of replicate analyses for these zooplankton samples was ± 0.55 per mil for d15N of zooplankton. The standard deviations of replicate analyses for d13C of zooplankton was not able to be calculated due lack of additional sample material.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1459536 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1459536
completed
Andréa G. Grottoli
Ohio State University
614-292-5782
School of Earth Sciences 125 South Oval Mall
Columbus
OH
43210
United States
grottoli.1@osu.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Sample
Sample_Type
Location
Site
Lat
Lon
Collection_Date
Analysis_Date
d13C
Carbon
d15N
Nitrogen
PDZ Europa ANCA-GSL elemental analyzer (Sercon Ltd., Cheshire, UK)
Thermo Finnigan Delta Plus XL continuous flow mass spectrometer and ThermoFinnigan GasBench II
PDZ Europa 20-20 isotope ratio mass spectrometer (Sercon Ltd., Cheshire, UK)
OI Analytical 1030D Total Organic Carbon Analyzer
theme
None, User defined
sample identification
sample type
region
site
latitude
longitude
date
d13C
Elemental carbon (C)
d15N
Nitrogen
featureType
BCO-DMO Standard Parameters
CHN Elemental Analyzer
Continuous Flow Interface for Mass Spectrometers
Isotope-ratio Mass Spectrometer
Total Organic Carbon Analyzer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Phenotype and genotype of coral adaptation and acclimatization to global change
https://osprey.bco-dmo.org/project/546273
Phenotype and genotype of coral adaptation and acclimatization to global change
<p><strong>Project Summary:</strong><br />
<em>Overview: </em>This study proposes to evaluate the adaptation and acclimatization capacity of eight species of Hawaiian corals to long-term exposure of elevated temperature and ocean acidification (OA) conditions using a two-part approach: 1) a survey of natural corals found across natural temperature and pCO2 gradients and 2) a two-year long mesocosm study which will expose corals collected in part 1 to a range of temperature and pCO2 conditions expected this century. In both approaches, the phenotypic (i.e., physiological and biogeochemical) responses of corals to future climate change will be measured in conjunction with the already funded genotypic (i.e., genomic and transcriptomic) responses of the same corals by Dr. Rob Toonen. This study will address variation at both the population and species level. It will also be the first study to examine the effects of elevated temperature and pCO2 on corals in replicated mesocosms over a multiannual timeframe with a comprehensive suite of physiological, biogeochemical, and genomic tools.</p>
<p><em>Intellectual Merit: </em>Coral reefs are among the most diverse ecosystems on the planet, housing an estimated 25% of marine species. Yet, they appear to be especially susceptible to the effects of climate change and ocean acidification. To date, the assumption has been that corals will not be able to adapt because the rates of anthropogenically driven ocean acidification and climate change are too high. But there is little experimental evidence to evaluate that assumption. Recent models highlight the critical importance of that assumption in determining coral extinction risk, and several recent studies (including a couple of recent ones from Grottoli’s group) indicate that previous studies may have underestimated the potential for corals to acclimatize or adapt to global change. Here, quantitative, empirical estimates of the potential for long-term coral acclimatization and adaptation under global change scenarios will be made. The proposed study includes ~97% of the corals in the Hawaiian archipelago, yielding extensive spatial and biological relevance for the study. Lastly, this research brings together the expertise of Grottoli at OSU (coral physiologist and biogeochemist), Toonen at UH (marine molecular biologist), and McCulloch at UWA (geochemist) in a unique collaboration that blends a large suite of genetic, physiological, and biogeochemical tools to build an unprecedentedly comprehensive picture of coral adaptation and acclimation to global change. Thus, this work has the potential to transform our conceptual and empirical understanding of how corals respond to rapid environmental change.</p>
<p><em>Broader Impacts:</em> Half of the species in the Hawaiian archipelago are endemic, making Hawaiian coral reefs a high priority for biodiversity conservation. Results from the proposed work will be used for adaptive management plans that collaborator Dr. Toonen is involved in with the goal of preserving Hawaiian coral biodiversity in a UNESCO World Heritage Site -- the Papahanaumokuakea Marine National Monument (PMNM). PMNM encompasses the northwestern Hawaiian Islands, is renowned as one of the most pristine and highly protected coral reefs remaining on the planet, is the single largest conservation area under the U.S. flag, and one of the largest marine conservation areas in the world. This project will provide a bridge between short-term, single-species studies and longer-term, multi-species responses to global change in reef community settings. Findings from this work will be communicated at scientific meetings, through peer-reviewed journal publications, and via press releases. Grottoli will also bring her research and enthusiasm for marine science into her classrooms and onto the podium when giving general audience and professional talks. She has an established track record of recruiting and promoting under-represented students and will continue to do so. This project will recruit 3 undergrads and 2 high school students for supported senior thesis/independent research and provide an environment that will foster their passion and skills necessary to pursue career options in STEM disciplines.</p>
Coral Adaptation
largerWorkCitation
project
eng; USA
oceans
-158.132
-157.683
21.2861
21.5942
2017-12-06
2017-12-16
Oahu, HI and Hawaii Institute of Marine Biology
0
BCO-DMO catalogue of parameters from Carbon and nitrogen stable isotopes of marine inorganic and organic matter around O'ahu, Hawai'i, December 2017
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/844842.rdf
Name: Sample
Units: unitless
Description: <p>sample identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/844843.rdf
Name: Sample_Type
Units: unitless
Description: <p>sample type: DIC; DOC; Zooplankton</p>
http://lod.bco-dmo.org/id/dataset-parameter/844844.rdf
Name: Location
Units: unitless
Description: <p>general location of sampling</p>
http://lod.bco-dmo.org/id/dataset-parameter/844845.rdf
Name: Site
Units: unitless
Description: <p>collection site</p>
http://lod.bco-dmo.org/id/dataset-parameter/844846.rdf
Name: Lat
Units: decimal degrees
Description: <p>latitude; north is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/844847.rdf
Name: Lon
Units: decimal degrees
Description: <p>longitude; east is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/844848.rdf
Name: Collection_Date
Units: unitless
Description: <p>collection date; local time</p>
http://lod.bco-dmo.org/id/dataset-parameter/844849.rdf
Name: Analysis_Date
Units: unitless
Description: <p>analysis date; local time</p>
http://lod.bco-dmo.org/id/dataset-parameter/844850.rdf
Name: d13C
Units: per mil
Description: <p>a measure of the ratio of stable isotopes of carbon 13C : 12C</p>
http://lod.bco-dmo.org/id/dataset-parameter/844851.rdf
Name: Carbon
Units: micrograms/liter
Description: <p>carbon concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/844852.rdf
Name: d15N
Units: per mil
Description: <p>a measure of the ratio of the stable isotopes of nitrogen 15N:14N</p>
http://lod.bco-dmo.org/id/dataset-parameter/844853.rdf
Name: Nitrogen
Units: micrograms/liter
Description: <p>nitrogen concentration</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
12880
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/6fde9fdb-3108-564a-b8fe-982164675ac3/content
download
https://doi.org/10.26008/1912/bco-dmo.844810.1
download
onLine
dataset
<p>Dissolved inorganic carbon (DIC) – 20 ml of seawater was filtered (0.45 µm pore size) and preserved in a glass crimp-top bottle with 200 µl of dried mercuric chloride for subsequent isotope analysis. The remaining seawater collected from each site was pre-filtered through 55 µm nylon mesh to remove large particulates and zooplankton. The d13C of DIC samples was analyzed at the Duke University Environmental Isotope Laboratory using a Thermo Finnigan Delta Plus XL continuous flow mass spectrometer via a ThermoFinnigan GasBench II and reported in permil relative to V-PDB. Repeated measurements of internal standards had a standard deviation of ± 0.2 per mil.</p>
<p>Dissolved organic carbon (DOC) – 40 ml of seawater was filtered through a quartz microfiber filter into a polytetrafluoroethylene bottle and acidified using 10% HCl to reach a pH below 2 to preserve the sample. The d13C of DOC samples was analyzed using an OI Analytical 1030D Total Organic Carbon Analyzer in the Aquatic Biogeochemical Laboratory at North Carolina State University and reported in permil relative to V-PDB. Repeated measurements of external standards had a standard deviation of ± 0.4 per mil.</p>
<p>Particulate organic matter (POM) – Between 3 – 4 L of the seawater was filtered through a quartz microfiber&nbsp; filter until the filter became light brown in color and was immediately stored at –20 degrees C for subsequent isotopic analysis of particulate organic carbon (POC) and particulate organic nitrogen (PON). The POM filters were fumigated with 12 M hydrochloric acid to remove carbonates (Moyer et al. 2013). All samples were combusted using a PDZ Europa ANCA-GSL elemental analyzer interfaced to a PDZ Europa 20-20 isotope ratio mass spectrometer (Sercon Ltd., Cheshire, UK) at the University of California (UC) Davis Stable Isotope Facility. The d13C and d15N values are reported in permil relative to V-PDB and air, respectively. The standard deviation of replicate analyses for these POM samples was ± 0.31 per mil&nbsp;for d13C. The standard deviations of replicate analyses for d15N of POM was not able to be calculated due lack of additional sample material.</p>
<p>Zooplankton – Zooplankton were collected using a bucket with an illuminated dive torch affixed to the bottom, which was placed on the seafloor near the reef at 1 m depth for 5 min. The zooplankton were separated into 150 – 400 µm and 400 – 800 µm size fractions in the field, stored on ice, and then isolated onto a glass fiber filter and stored at –20 degrees&nbsp;C upon return to the lab the same day. The zooplankton samples were processed separately for d13C and d15N. For d13C, zooplankton samples were fumigated with 12 M hydrochloric acid to remove carbonates. The samples were processed for d15N without pretreatment to ensure that these isotope values were unaffected by acidification. All samples were combusted using a PDZ Europa ANCA-GSL elemental analyzer interfaced to a PDZ Europa 20-20 isotope ratio mass spectrometer (Sercon Ltd., Cheshire, UK) at the University of California (UC) Davis Stable Isotope Facility. The d13C and d15N values are reported in permil relative to V-PDB and air, respectively. The standard deviation of replicate analyses for these zooplankton samples was ± 0.55 per mil for d15N of zooplankton. The standard deviations of replicate analyses for d13C of zooplankton was not able to be calculated due lack of additional sample material.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:</strong><br />
- data submitted in Excel file 'Source Isotope Data for BCO-DMO_Price et al.xlsx' sheet 'Data for BCO_DMO'<br />
- added conventional header with dataset name, PI name, version, date<br />
- renamed columns to conform with BCO-DMO naming conventions (removed units, spaces,&nbsp;special characters)<br />
- re-formatted dates from d-Mon-yy to yyyy-mm-dd<br />
- split Coordinates column into Lat and Lon<br />
- changed special characters: ‘ to ' and ā to a, µ to u</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PDZ Europa ANCA-GSL elemental analyzer (Sercon Ltd., Cheshire, UK)
PDZ Europa ANCA-GSL elemental analyzer (Sercon Ltd., Cheshire, UK)
PI Supplied Instrument Name: PDZ Europa ANCA-GSL elemental analyzer (Sercon Ltd., Cheshire, UK) PI Supplied Instrument Description:Used for Particulate Organic Matter (POM) analysis in zooplankton samples. Instrument Name: CHN Elemental Analyzer Instrument Short Name:CHN_EA Instrument Description: A CHN Elemental Analyzer is used for the determination of carbon, hydrogen, and nitrogen content in organic and other types of materials, including solids, liquids, volatile, and viscous samples.
Thermo Finnigan Delta Plus XL continuous flow mass spectrometer and ThermoFinnigan GasBench II
Thermo Finnigan Delta Plus XL continuous flow mass spectrometer and ThermoFinnigan GasBench II
PI Supplied Instrument Name: Thermo Finnigan Delta Plus XL continuous flow mass spectrometer and ThermoFinnigan GasBench II PI Supplied Instrument Description:Used for Dissolved Inorganic Carbon (DIC) analysis. Instrument Name: Continuous Flow Interface for Mass Spectrometers Instrument Short Name: Instrument Description: A Continuous Flow Interface connects solid and liquid sample preparation devices to instruments that measure isotopic composition. It allows the introduction of the sample and also reference and carrier gases.
Examples: Finnigan MATConFlo II, ThermoScientific ConFlo IV, and Picarro Caddy.
Note: This is NOT an analyzer
PDZ Europa 20-20 isotope ratio mass spectrometer (Sercon Ltd., Cheshire, UK)
PDZ Europa 20-20 isotope ratio mass spectrometer (Sercon Ltd., Cheshire, UK)
PI Supplied Instrument Name: PDZ Europa 20-20 isotope ratio mass spectrometer (Sercon Ltd., Cheshire, UK) PI Supplied Instrument Description:Used for Particulate Organic Matter (POM) analysis in zooplankton samples. Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
OI Analytical 1030D Total Organic Carbon Analyzer
OI Analytical 1030D Total Organic Carbon Analyzer
PI Supplied Instrument Name: OI Analytical 1030D Total Organic Carbon Analyzer PI Supplied Instrument Description:Used for Dissolved Organic Carbon (DOC) analysis. Instrument Name: Total Organic Carbon Analyzer Instrument Short Name:TOC analyzer Instrument Description: A unit that accurately determines the carbon concentrations of organic compounds typically by detecting and measuring its combustion product (CO2). See description document at: http://bcodata.whoi.edu/LaurentianGreatLakes_Chemistry/bs116.pdf Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/