http://lod.bco-dmo.org/id/dataset/846664
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2021-03-23
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Eukaryotic phytoplankton flow cytometric results from salp grazing incubations conducted on R/V Tangaroa cruise TAN1810 during Oct-Nov 2018
2021-04-08
publication
2021-04-08
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2022-09-13
publication
https://doi.org/10.26008/1912/bco-dmo.846664.1
Michael R. Stukel
Florida State University
principalInvestigator
Moira Decima
University of California-San Diego Scripps
principalInvestigator
Karen E. Selph
University of Hawaiʻi at Mānoa
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Stukel, M., Selph, K. E., Decima, M. (2022) Eukaryotic phytoplankton flow cytometric results from salp grazing incubations conducted on R/V Tangaroa cruise TAN1810 during Oct-Nov 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-04-08 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.846664.1 [access date]
Eukaryotic Phytoplankton Flow Cytometry from Salp Incubations Dataset Description: Methods and Sampling: <p>This dataset seeks to quantify food web flows through contrasting salp-dominated and salp-absent water parcels near the Chatham Rise off western New Zealand where salp blooms are a predictable phenomenon.</p>
<p>Paired 20-liter plankton kreisels or 30-liter pseudo-kreisels were gently filled (using silicon tubing) with mixed layer seawater collected by CTD-Niskin rosette casts at ~22:00 local time. Salps for incubations were collected at ~23:00 local time using the salp net. The net was towed slowly and briefly (5-10 minutes) through the mixed layer. Healthy specimens, i.e., those that showed no physical damage, were then gently transferred (using a large ladle) into a bucket containing filtered surface seawater Specimens were further observed (15-30 minutes) to ensure they actively swam (i.e., that they appeared healthy). They were then transferred into one of the paired kreisels (+Salp treatment), while the second kreisel was used as a control treatment with prey only. We successfully collected and incubated S. thompsoni blastozooids and oozooids ranging in size from 50 – 128 millimeters. We also conducted three incubations with a chain of blastozooids (6-8 millimeter individuals) released by an oozooid inside of one of the plankton kreisels. We found that this was the only way to successfully obtain such small blastozooids in healthy conditions. We also incubated a 112-millimeter Thetys vagina oozooid.</p>
<p>Water was circulated within the kreisels using a peristaltic pump and silicon tubing. Just after salp transfer to the kreisels, initial samples for flow cytometry were taken from each experimental and control kreisel. Additional time points were taken approximately every 2 hours and analyzed in near real time to allow us to monitor salp grazing.Incubations typically lasted ~24 hours.</p>
<p>Samples from the salp incubations were analyzed at sea on a Becton-Dickinson Accuri C6 Plus flow cytometer to estimate the abundance and size of eukaryotic phytoplankton. Samples (~660 µL) were run live within ~1-2 h of collection, discriminating on the Chl a fluorescence signal. We estimated cell diameter from forward light scatter after developing a relationship based on analyses of multiple polystyrene bead sizes (0.99-10 µm diameter). We note that forward scatter is an imperfect proxy for equivalent spherical diameter, and thus the absolute cell sizes determined in our study should be assumed to have associated uncertainty.</p>
<p>For additional details on all methods, see Stukel et al. (in review).</p>
<p>Note: This dataset is also provided in .mat (MATLAB) format under Supplemental Files.&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1756465 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1756465
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1756610 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1756610
completed
Michael R. Stukel
Florida State University
815-258-3875
Earth, Ocean, and Atmos. Science Building (EOA) Rm. 6089 1011 Academic Way
Tallahassee
FL
32306
USA
mstukel@fsu.edu
pointOfContact
Moira Decima
University of California-San Diego Scripps
858-246-4710
9500 Gilman Dr #0218
La Jolla
CA
92093-0218
USA
mdecima@ucsd.edu
pointOfContact
Karen E. Selph
University of Hawaiʻi at Mānoa
808-956-7941
Department of Oceanography 1000 Pope Road, MSB 205
Honolulu
HI
96822
USA
selph@hawaii.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Stage
Incubation
SalpTL
NumSalps
Temperature
Vol_Kreisel
TimePoint
TimeSinceStart
TreatmentControl
vol_FCM
FSC
SSC
Phyco
Chl
ESD_fsc
salp net
theme
None, User defined
stage
number
length
temperature of sample or a generic measurement (not in-situ water temp)
volume
time_point
incubation time or duration
treatment
No BCO-DMO term
diameter
featureType
BCO-DMO Standard Parameters
Flow Cytometer
Niskin bottle
Plankton Net
instrument
BCO-DMO Standard Instruments
TAN1810
service
Deployment Activity
Chatham Rise (Subtropical and Sub-Antarctic waters off of New Zealand)
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Quantifying trophic roles and food web ecology of salp blooms of the Chatham Rise
https://osprey.bco-dmo.org/project/754878
Collaborative Research: Quantifying trophic roles and food web ecology of salp blooms of the Chatham Rise
<p><em>NSF Award Abstract:</em><br />
Salps are unique open-ocean animals that range in size from a few millimeters to greater than twenty centimeters, have a gelatinous (jelly-like) body, and can form long chains of many connected individuals. These oceanic organisms act as oceanic vacuum cleaners, having incredibly high feeding rates on phytoplankton and, unusual for consumers of their size, smaller bacteria-sized prey. This rapid feeding and the salps' tendency to form dense blooms, allows them move substantial amounts of prey carbon from the surface into the deep ocean, leading to carbon dioxide removal from the atmosphere. However, salps are often considered a trophic dead-end, rather than a link, in the food web due to the assumption that they themselves are not consumed, since their gelatinous bodies are less nutritious than co-occurring crustacean prey. Along with this, salp populations are hypothesized to be increasing due to climate change. This proposal addresses these questions: 1) Do salps compete primarily with crustaceans (as in the prevailing paradigm) or are they competitors of single-celled protists, which are the dominant grazers of small phytoplankton? 2) Do salp blooms increase the efficiency of food-web pathways from tiny phytoplankton to fisheries production in nutrient-poor ocean regions?</p>
<p>This project will support the interdisciplinary education of a graduate student who will learn modeling and laboratory techniques in the fields of biological and chemical oceanography and stimulate international collaborations between scientists in the United States and New Zealand. Additionally, several Education and Outreach initiatives are planned, including development of a week-long immersive high school class in biological oceanography, and education modules that will serve the "scientists-in-the schools" program in Tallahassee, FL.</p>
<p>It is commonly assumed that salps are a trophic sink. However, this idea was developed before the discovery that protists (rather than crustaceans) are the dominant grazers in the open ocean and was biased by the difficulty of recognizing gelatinous salps in fish guts. More recent studies show that salps are found in guts of a diverse group of fish and seabirds and are a readily available prey source when crustacean abundance is low. This proposal seeks to quantify food web flows through contrasting salp-dominated and salp-absent water parcels near the Chatham Rise off western New Zealand where salp blooms are a predictable phenomenon. The proposal will leverage previously obtained data on salp abundance, bulk grazing impact, and biogeochemical significance during Lagrangian experiments conducted by New Zealand-based collaborators. The proposal will determine 1) taxon- and size-specific phytoplankton growth rate measurements, 2) taxon- and size-specific protozoan and salp grazing rate measurements, 3) compound specific isotopic analysis of the amino acids of mesozooplankton to quantify the trophic position of salps, hyperiid amphipods, and other crustaceans, 4) sediment traps to quantify zooplankton carcass sinking rates, and 5) linear inverse ecosystem modeling syntheses. Secondary production and trophic flows from this well-constrained ecosystem model will be compared to crustacean-dominated and microbial loop-dominated ecosystems in similarly characterized regions (California Current, Costa Rica Dome, and Gulf of Mexico).</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
Salp Food Web Ecology
largerWorkCitation
project
eng; USA
oceans
Chatham Rise (Subtropical and Sub-Antarctic waters off of New Zealand)
174.095
179.9428
-45.5503
-42.742
2018-10-23
2018-11-21
East of New Zealand, Chatham Rise area
0
BCO-DMO catalogue of parameters from Eukaryotic phytoplankton flow cytometric results from salp grazing incubations conducted on R/V Tangaroa cruise TAN1810 during Oct-Nov 2018
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/848639.rdf
Name: Stage
Units: unitless
Description: <p>Stage of salp (oozooid/blastozooid)</p>
http://lod.bco-dmo.org/id/dataset-parameter/848640.rdf
Name: Incubation
Units: number
Description: <p>Incubation Number</p>
http://lod.bco-dmo.org/id/dataset-parameter/848641.rdf
Name: SalpTL
Units: millimeters (mm)
Description: <p>Salp Total length</p>
http://lod.bco-dmo.org/id/dataset-parameter/848642.rdf
Name: NumSalps
Units: number
Description: <p>Number of salps in the incubation</p>
http://lod.bco-dmo.org/id/dataset-parameter/848643.rdf
Name: Temperature
Units: degrees Celsius
Description: <p>Temperature of incubation</p>
http://lod.bco-dmo.org/id/dataset-parameter/848644.rdf
Name: Vol_Kreisel
Units: liter
Description: <p>Volume of the plankton kreisel</p>
http://lod.bco-dmo.org/id/dataset-parameter/848645.rdf
Name: TimePoint
Units: unitless
Description: <p>Time point of sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/848646.rdf
Name: TimeSinceStart
Units: days
Description: <p>Time since the beginning of incubation</p>
http://lod.bco-dmo.org/id/dataset-parameter/848647.rdf
Name: TreatmentControl
Units: unitless
Description: <p>1=+Salp Treatment, 2=Control Treatment</p>
http://lod.bco-dmo.org/id/dataset-parameter/848648.rdf
Name: vol_FCM
Units: milliliters (mL)
Description: <p>Sample volume analyzed for FCM</p>
http://lod.bco-dmo.org/id/dataset-parameter/848649.rdf
Name: FSC
Units: arbitrary units
Description: <p>Forward scatter</p>
http://lod.bco-dmo.org/id/dataset-parameter/848650.rdf
Name: SSC
Units: arbitrary units
Description: <p>Side scatter</p>
http://lod.bco-dmo.org/id/dataset-parameter/848651.rdf
Name: Phyco
Units: arbitrary units
Description: <p>Phycoerythrin fluorescence</p>
http://lod.bco-dmo.org/id/dataset-parameter/848652.rdf
Name: Chl
Units: arbitrary units
Description: <p>Chlorophyll fluorescence</p>
http://lod.bco-dmo.org/id/dataset-parameter/848653.rdf
Name: ESD_fsc
Units: micrometer (um)
Description: <p>Equivalent spherical diameter estimated from FSC</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
312470184
https://darchive.mblwhoilibrary.org/bitstream/1912/29333/1/dataset-846664_eukaryotic-phytoplankton-flow-cytometry-salp-grazing-incubations__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.846664.1
download
onLine
dataset
<p>This dataset seeks to quantify food web flows through contrasting salp-dominated and salp-absent water parcels near the Chatham Rise off western New Zealand where salp blooms are a predictable phenomenon.</p>
<p>Paired 20-liter plankton kreisels or 30-liter pseudo-kreisels were gently filled (using silicon tubing) with mixed layer seawater collected by CTD-Niskin rosette casts at ~22:00 local time. Salps for incubations were collected at ~23:00 local time using the salp net. The net was towed slowly and briefly (5-10 minutes) through the mixed layer. Healthy specimens, i.e., those that showed no physical damage, were then gently transferred (using a large ladle) into a bucket containing filtered surface seawater Specimens were further observed (15-30 minutes) to ensure they actively swam (i.e., that they appeared healthy). They were then transferred into one of the paired kreisels (+Salp treatment), while the second kreisel was used as a control treatment with prey only. We successfully collected and incubated S. thompsoni blastozooids and oozooids ranging in size from 50 – 128 millimeters. We also conducted three incubations with a chain of blastozooids (6-8 millimeter individuals) released by an oozooid inside of one of the plankton kreisels. We found that this was the only way to successfully obtain such small blastozooids in healthy conditions. We also incubated a 112-millimeter Thetys vagina oozooid.</p>
<p>Water was circulated within the kreisels using a peristaltic pump and silicon tubing. Just after salp transfer to the kreisels, initial samples for flow cytometry were taken from each experimental and control kreisel. Additional time points were taken approximately every 2 hours and analyzed in near real time to allow us to monitor salp grazing.Incubations typically lasted ~24 hours.</p>
<p>Samples from the salp incubations were analyzed at sea on a Becton-Dickinson Accuri C6 Plus flow cytometer to estimate the abundance and size of eukaryotic phytoplankton. Samples (~660 µL) were run live within ~1-2 h of collection, discriminating on the Chl a fluorescence signal. We estimated cell diameter from forward light scatter after developing a relationship based on analyses of multiple polystyrene bead sizes (0.99-10 µm diameter). We note that forward scatter is an imperfect proxy for equivalent spherical diameter, and thus the absolute cell sizes determined in our study should be assumed to have associated uncertainty.</p>
<p>For additional details on all methods, see Stukel et al. (in review).</p>
<p>Note: This dataset is also provided in .mat (MATLAB) format under Supplemental Files.&nbsp;</p>
Specified by the Principal Investigator(s)
<p>BCO-DMO Processing:<br />
- Adjusted field/parameter names to comply with BCO-DMO naming conventions;<br />
- Added a conventional header with dataset name, PI names, version date.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: PI Supplied Instrument Description:Becton-Dickinson Accuri C6 Plus flow cytometer Instrument Name: Flow Cytometer Instrument Short Name:Flow Cytometer Instrument Description: Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/
PI Supplied Instrument Name: Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
salp net
salp net
PI Supplied Instrument Name: salp net Instrument Name: Plankton Net Instrument Short Name:Plankton Net Instrument Description: A Plankton Net is a generic term for a sampling net that is used to collect plankton. It is used only when detailed instrument documentation is not available. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/22/
Cruise: TAN1810
TAN1810
Community Standard Description
International Council for the Exploration of the Sea
R/V Tangaroa
vessel
TAN1810
Moira Decima
New Zealand National Institute of Water and Atmospheric Research
Community Standard Description
International Council for the Exploration of the Sea
R/V Tangaroa
vessel