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            <gco:CharacterString>Cite this dataset as: Anderson, S. I., Franze, G., Kling, J. D., Wilburn, P., Kremer, C. T., Menden-Deuer, S., Litchman, E., Hutchins, D. A., Rynearson, T. A. (2021) Microscopy cell counts from multivariate mesocosm experiments conducted with a natural phytoplankton community from Narragansett Bay, RI. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-04-14 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.848977.1 [access date]</gco:CharacterString>
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        <gco:CharacterString>Methods and Sampling: &amp;lt;p&amp;gt;Multivariate mesocosm experiments were conducted with a natural phytoplankton community from Narragansett Bay, RI. Water was incubated in triplicate at -0.5ºC, 2.6ºC, and 6ºC for 10 days. At each temperature, treatments included both nutrient amendments (N, P, Si addition) and controls (no macronutrients added).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;At the onset and conclusion of the incubation experiments, the microplankton community was identified and quantified to discern treatment effects on community composition. Aliquots from each incubation were fixed in triplicate with 2% acid Lugol’s solution for microscopy, final concentration. Cell enumeration was conducted by performing cell counts ~&amp;amp;gt;10 micrometer (µm) on a 1 milliliter (ml) Sedgewick cell-counting chamber (Structure Probe Inc.) using an Eclipse E800 microscope (Nikon).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;These data were assessed as part of “The Interactive Effects of Temperature and Nutrients on a Spring Phytoplankton Community” (Anderson et al, in prep).&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/712795.rdf" xlink:title="OCE-1638834" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1638834 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1638834</gmx:Anchor>
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            <gmx:Anchor xlink:href="https://ror.org/013ckk937" xlink:title="ROR ID" xlink:actuate="onRequest">University of Rhode Island</gmx:Anchor>
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Photosynthetic marine microbes, phytoplankton, contribute half of global primary production, form the base of most aquatic food webs and are major players in global biogeochemical cycles. Understanding their community composition is important because it affects higher trophic levels, the cycling of energy and elements and is sensitive to global environmental change. This project will investigate how phytoplankton communities respond to two major global change stressors in aquatic systems: warming and changes in nutrient availability. The researchers will work in two marine systems with a long history of environmental monitoring, the temperate Narragansett Bay estuary in Rhode Island and a subtropical North Atlantic site near Bermuda. They will use field sampling and laboratory experiments with multiple species and varieties of phytoplankton to assess the diversity in their responses to different temperatures under high and low nutrient concentrations. If the diversity of responses is high within species, then that species may have a better chance to adapt to rising temperatures and persist in the future. Some species may already be able to grow at high temperatures; consequently, they may become more abundant as the ocean warms. The researchers will incorporate this response information in mathematical models to predict how phytoplankton assemblages would reorganize under future climate scenarios. Graduate students and postdoctoral associates will be trained in diverse scientific approaches and techniques such as shipboard sampling, laboratory experiments, genomic analyses and mathematical modeling. The results of the project will be incorporated into K-12 teaching, including an advanced placement environmental science class for underrepresented minorities in Los Angeles, data exercises for rural schools in Michigan and disseminated to the public through an environmental journalism institute based in Rhode Island.&lt;/p&gt;
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	Description: &lt;p&gt;Treatment identification. Letter corresponds to temperature treatment, L=low (-5_C), M=medium (2.6_C), H=high (6_C); and symbol describes whether macronutrients were added (+) or not (-). T0 is initial community.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850871.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/850872.rdf
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	Units: degrees celcius (¡C)
	Description: &lt;p&gt;Nutrient treatment in which incubation was conducted&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850873.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/850874.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/850875.rdf
	Name: Alexandrium_sp
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850876.rdf
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	Units: cells per liter (cells/L)
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http://lod.bco-dmo.org/id/dataset-parameter/850877.rdf
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	Units: cells per liter (cells/L)
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http://lod.bco-dmo.org/id/dataset-parameter/850878.rdf
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	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850879.rdf
	Name: Chaetoceros_affinis
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850880.rdf
	Name: Chaetoceros_atlanticus
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850881.rdf
	Name: Chaetoceros_compressus
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850882.rdf
	Name: Chaetoceros_costatus
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850883.rdf
	Name: Chaetoceros_danicus
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850884.rdf
	Name: Chaetoceros_debilis
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850885.rdf
	Name: Chaetoceros_decipiens
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850886.rdf
	Name: Chaetoceros_diadema
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850887.rdf
	Name: Chaetoceros_didymus
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850888.rdf
	Name: Chaetoceros_laciniosus
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850889.rdf
	Name: Chaetoceros_mitra
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850890.rdf
	Name: Chaetoceros_similis
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850891.rdf
	Name: Chaetoceros_spp
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850892.rdf
	Name: Chaetoceros_subtilis
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850893.rdf
	Name: Chaetoceros_winghamii
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850894.rdf
	Name: Coscinodiscus_spp
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850895.rdf
	Name: Cylindrotheca_closterium
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850896.rdf
	Name: Dactyliosolen_blavyanus
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850897.rdf
	Name: Dactyliosolen_fragilissimus
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850898.rdf
	Name: Diatom_unknown
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850899.rdf
	Name: Dictyocha_speculum
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850900.rdf
	Name: Dinoflagellates_unknown
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850901.rdf
	Name: Ditylum_brightwellii
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850902.rdf
	Name: Flagellate_unknown
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850903.rdf
	Name: Guinardia_delicatula
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850904.rdf
	Name: Gymnodinium_spp
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850905.rdf
	Name: Gyrodinium_spp
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850906.rdf
	Name: Heterocapsa_rotundata
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850907.rdf
	Name: Heterocapsa_triquetra
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850908.rdf
	Name: Leptocylindrus_danicus
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850909.rdf
	Name: Leptocylindrus_minimus
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850910.rdf
	Name: Licmophora_spp
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850911.rdf
	Name: Melosira_spp
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850912.rdf
	Name: Odontella_spp
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850913.rdf
	Name: Paralia_sulcata
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850914.rdf
	Name: Pennate_unknown
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850915.rdf
	Name: Phaeocystis_spp
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850916.rdf
	Name: Pleurosigma_spp
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850917.rdf
	Name: Prorocentrum_spp
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850918.rdf
	Name: Protoperidinium_spp
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850919.rdf
	Name: Pseudonitzschia_spp
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850920.rdf
	Name: Rhabdonema_adriaticum
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850921.rdf
	Name: Rhizosolenia_pungens
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850922.rdf
	Name: Rhizosolenia_setigera
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850923.rdf
	Name: Rhizosolenia_spp
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850924.rdf
	Name: Rhizosolenia_styliformis_imbricata
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850925.rdf
	Name: Skeletonema_spp
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850926.rdf
	Name: Thalassionema_nitzschioides
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850927.rdf
	Name: Thalassiosira_nordenskioeldii
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850928.rdf
	Name: Thalassiosira_rotula
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/850929.rdf
	Name: Thalassiosira_spp
	Units: cells per liter (cells/L)
	Description: &lt;p&gt;Species abundance&lt;/p&gt; 
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                      <gmd:date gco:nilReason="unknown"/>
                    </gmd:CI_Citation>
                  </gmd:sourceCitation>
                </gmd:LI_Source>
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            </gmd:LI_ProcessStep>
          </gmd:processStep>
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            <gmd:LI_ProcessStep>
              <gmd:description>
                <gco:CharacterString>&amp;lt;p dir=&amp;quot;ltr&amp;quot;&amp;gt;BCO-DMO processing description:&amp;lt;/p&amp;gt;

&amp;lt;p dir=&amp;quot;ltr&amp;quot;&amp;gt;- Adjusted field/parameter names to comply with database requirements&amp;lt;br /&amp;gt;
- Missing data identifier ‘NA’ and ‘N/A’ replaced with 'nd' (BCO-DMO's default missing data identifier)&amp;lt;br /&amp;gt;
- Added a conventional header with dataset name, PI names, version date&amp;lt;/p&amp;gt;</gco:CharacterString>
              </gmd:description>
              <gmd:source>
                <gmd:LI_Source>
                  <gmd:sourceCitation>
                    <gmd:CI_Citation>
                      <gmd:title>
                        <gco:CharacterString>Specified by the Principal Investigator(s)</gco:CharacterString>
                      </gmd:title>
                      <gmd:date gco:nilReason="unknown"/>
                    </gmd:CI_Citation>
                  </gmd:sourceCitation>
                </gmd:LI_Source>
              </gmd:source>
            </gmd:LI_ProcessStep>
          </gmd:processStep>
        </gmd:LI_Lineage>
      </gmd:lineage>
   </gmd:DQ_DataQuality>
  </gmd:dataQualityInfo>
  <gmd:metadataMaintenance>
    <gmd:MD_MaintenanceInformation>
      <gmd:maintenanceAndUpdateFrequency>
        <gmd:MD_MaintenanceFrequencyCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#MD_MaintenanceFrequencyCode" codeListValue="asNeeded" codeSpace="009">asNeeded</gmd:MD_MaintenanceFrequencyCode>
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        <gco:CharacterString>7.x-1.1</gco:CharacterString>
      </gmd:maintenanceNote>
      <gmd:contact>
        <gmd:CI_ResponsibleParty>
  <gmd:organisationName>
    <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/affiliation/191.rdf" xlink:actuate="onRequest">Biological and Chemical Oceanography Data Management Office (BCO-DMO)</gmx:Anchor>
  </gmd:organisationName>
  <gmd:contactInfo>
    <gmd:CI_Contact>
		  <gmd:phone>
		    <gmd:CI_Telephone>
				  <gmd:voice>
				    <gco:CharacterString>Unavailable</gco:CharacterString>
				  </gmd:voice>
				  <gmd:facsimile>
				    <gco:CharacterString>508-289-2009</gco:CharacterString>
				  </gmd:facsimile>
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		  </gmd:phone>
		  <gmd:address>
		    <gmd:CI_Address>
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				    <gco:CharacterString>WHOI MS#36</gco:CharacterString>
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				  <gmd:city>
				    <gco:CharacterString>Woods Hole</gco:CharacterString>
				  </gmd:city>
				  <gmd:administrativeArea>
				    <gco:CharacterString>MA</gco:CharacterString>
				  </gmd:administrativeArea>
				  <gmd:postalCode>
				    <gco:CharacterString>02543</gco:CharacterString>
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				  <gmd:country>
				    <gco:CharacterString>USA</gco:CharacterString>
				  </gmd:country>
				  <gmd:electronicMailAddress>
				    <gco:CharacterString>info@bco-dmo.org</gco:CharacterString>
				  </gmd:electronicMailAddress>
		    </gmd:CI_Address>
		  </gmd:address>
      <gmd:onlineResource>
          <gmd:CI_OnlineResource>
            <gmd:linkage>
              <gmd:URL>http://www.bco-dmo.org</gmd:URL>
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          </gmd:CI_OnlineResource>
        </gmd:onlineResource>
		  <gmd:hoursOfService>
        <gco:CharacterString>Monday - Friday 8:00am - 5:00pm</gco:CharacterString>
      </gmd:hoursOfService>
		  <gmd:contactInstructions>
		    <gco:CharacterString>For questions regarding this resource, please contact BCO-DMO via the email address provided.</gco:CharacterString>
		  </gmd:contactInstructions>
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</gmd:CI_ResponsibleParty>
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    </gmd:MD_MaintenanceInformation>
  </gmd:metadataMaintenance>
  <gmi:acquisitionInformation>
    <gmi:MI_AcquisitionInformation>
    <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/708.rdf" xlink:title="Microscope - Optical" xlink:actuate="onRequest">Eclipse E800 microscope (Nikon)</gmx:Anchor>
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            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>Eclipse E800 microscope (Nikon)</gco:CharacterString>
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          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Eclipse E800 microscope (Nikon) Instrument Name: Microscope - Optical Instrument Short Name:   Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a &quot;light microscope&quot;. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/</gco:CharacterString>
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      </gmi:instrument>
      </gmi:MI_AcquisitionInformation>
  </gmi:acquisitionInformation>
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