http://lod.bco-dmo.org/id/dataset/849153
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2021-04-15
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Direct measure of phytoplankton cell quotas from field populations sampled from multiple cruises cruises between 2010 and 2016
2021-06-02
publication
2021-06-02
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2021-06-03
publication
https://doi.org/10.26008/1912/bco-dmo.849153.1
Michael W. Lomas
Bigelow Laboratory for Ocean Sciences
principalInvestigator
Adam Martiny
University of California-Irvine
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Lomas, M. W., Martiny, A. (2021) Direct measure of phytoplankton cell quotas from field populations sampled from multiple cruises cruises between 2010 and 2016. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-06-02 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.849153.1 [access date]
Sorted Cell Quotas Dataset Description: Methods and Sampling: <p><em>Phytoplankton sample collection and preparation. </em>Samples for taxon-specific elemental content were collected using 12 liter (L) Niskin bottles, usually from two depths at each station (see supplemental file for map of stations from which samples were collected and cell-sorted to quantify cellular macronutrient content; one within the upper mixed layer (10-20 meters) and one near the deep chlorophyll maximum. Due to timing of the cruises, samples were collected from a seasonal window limited to summer and early fall. Samples were prepared for sorting by concentrating cells from the ambient sample under darkened conditions using a Memteq High Volume Cell Trap (0.2 micrometer pore size), and then flushed from the Cell Trap using 0.2 micrometer (µm) filtered seawater and a luer lock syringe; roughly 3-4 liters (L) of ambient seawater was concentrated down to ~5 milliliters (mls). Samples were either gravity filtered through the Cell Trap directly from the Niskin bottle or pushed through the cell trap using a peristaltic pump at &lt;50 milliliter per minute (ml min<sup>-1</sup>). Samples were then kept cool at 4 degrees C if they were going to be sorted live and immediately after collection or fixed with freshly filtered paraformaldehyde (0.5% v/v final concentration) if they were going to be stored for later sorting. Samples were allowed to fix at 4<sup> </sup>degrees C for 1-2 hours and then flash frozen in liquid nitrogen and then moved to -80<sup> </sup>degrees C for longer term storage.</p>
<p><em>Cell sorting and elemental analysis. </em>The cell counting and sorting protocols used follow that of Baer et al. (2017). Cell counts and sorts were performed on either a Becton Dickinson Influx or FACSJazz flow cytometer, each utilizing a 200 milliwatt (mW) 488 namometer (nm) laser, with detectors for forward scatter, side scatter, 692 namometer (nm), and 530 namometer (nm), and operated with 8 grams of NaCL kg<sup>-1</sup> solution for sheath fluid, which was filtered inline using a 0.22 micrometer (µm) Millipore Sterivex<sup>TM</sup> filter. Instrument alignment was performed with 3.0 micrometer (μm) 6-peak rainbow beads, while roughly hourly checks on forward scatter response were performed with 0.53 micrometer (μm) Nile Red beads (Spherotech). <em>Prochlorococcus</em> populations were discriminated based on forward scatter and red fluorescence, and a gate in orange (530 nm) discriminated for <em>Synechococcus</em>. Eukaryotes were all larger autofluorescing cells that did not fit the cyanobacterial gating scheme. For sorting, sort control software was set to "1.0 drop pure" sort mode. In excess of 17 million, 8 million, and 500,000 cells for <em>Prochlorococcus, Synechococcus, </em>and eukaryotes were sorted for chemical analysis, respectively. Not all phytoplankton groups were sufficiently abundant at each station/depth for practical sorting and subsequent analysis. Post-sort purity tests were run with subsamples of each sorted population; sort purity always exceeded 94%. Sorted populations were collected in polystyrene Falcon tubes (BD Biosciences Inc.) and subsequently filtered on pre-combusted (450 degrees C for 4 hours) GF-75 filters (Ahlstrom; nominal pore size = 0.3 micrometer). To ensure complete capture of all of the cells, the Falcon tubes were rinsed multiple times with 0.2 micrometer (μm) filtered sheath fluid. Filter and sheath fluid blanks were produced each day samples were sorted for subsequent subtraction from the mass of each unknown sample. Following filtration, filters were placed in acid-washed cryovials and frozen (-20 degrees C) until analysis as described below. Average cellular elemental content was determined by dividing the elemental content of the sample (see next section) by the number of sorted cells as determined in a direct post-sort count analysis of the sorted sample.</p>
<p><em>Particulate nutrients. </em>Particulate organic P (POP) was analyzed using the ash-hydrolysis method, with oxidation efficiency and standard recovery tested with each sample run using an ATP standard solution and a certified phosphate standard (OSIL Phosphate Nutrient Standard Solution). Method precision is 1-2% at 5 nanomoles per kilogram (nmol kg<sup>-1</sup>). Samples for POC and PON were acid fumed in a desiccator over concentrated HCl prior to analysis. After acid fuming, POC and PON were determined on a Costech 4010 elemental analyzer or a Control Equipment 440 elemental analyzer, depending on the samples. L-glutamic acid (USGS40) was used for standard curve generation and as a check standard (tolerance of &lt;0.1 microgram) approximately every ten samples and at the end of every instrument run. Empty tin capsules (Costech Analytical Technologies) were cleaned with acetone and dried and run as instrument blanks.</p>
<p>Additional methodological details can be found in Lomas et al. 2021 (currently in revision).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1046297 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1046297
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1045966 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1045966
completed
Michael W. Lomas
Bigelow Laboratory for Ocean Sciences
207-315-2567 ext 311
60 Bigelow Drive PO Box 380
East Boothbay
ME
04544
United States
mlomas@bigelow.org
pointOfContact
Adam Martiny
University of California-Irvine
949-824-9713
Earth System Science & Ecology and Evolutionary Biology 3208 Croul Hall
Irvine
CA
92697
USA
amartiny@uci.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Categorical_Region
Cruise_ID
Date
Latitude
Longitude
Station
Depth
Prochlorococcus_POC
Prochlorcoccus_PON
Prochlorococcus_POP
Synechococcus_POC
Synechococcus_PON
Synechococcus_POP
Eukaryote_POC
Eukaryote_PON
Eukaryote_POP
Costech 4010 elemental analyzer, Control Equipment 440 elemental analyzer
theme
None, User defined
region
Cruise identfier
date
latitude
longitude
station
depth
particulate organic Carbon (POC)
particulate organic nitrogen
particulate organic phosphorus
featureType
BCO-DMO Standard Parameters
CHN Elemental Analyzer
Flow Cytometer
Niskin bottle
instrument
BCO-DMO Standard Instruments
AE1319
NH1410
NH1418
RR1604
SO243
AE1032
AE1226
service
Deployment Activity
NW Atlantic
Eastern Tropical North Pacific (ETNP) oxygen minimum zone off Manzanillo, Mexico; sampling transects run southeast-northwest along the Colima coast (~19-24°N, 106-116°W) and northeast-southwest off the coast (~14-18°N, 106-115°W)
Hawaii
Hydrographic line IO9, Indian Ocean
Western Subtropical North Atlantic, ~32N - ~19N, ~65W
St. George's, Bermuda
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Dimensions of Biodiversity
http://www.nsf.gov/funding/pgm_summ.jsp?pims_id=503446
Dimensions of Biodiversity
(adapted from the NSF Synopsis of Program)
Dimensions of Biodiversity is a program solicitation from the NSF Directorate for Biological Sciences. FY 2010 was year one of the program. [MORE from NSF]
The NSF Dimensions of Biodiversity program seeks to characterize biodiversity on Earth by using integrative, innovative approaches to fill rapidly the most substantial gaps in our understanding. The program will take a broad view of biodiversity, and in its initial phase will focus on the integration of genetic, taxonomic, and functional dimensions of biodiversity. Project investigators are encouraged to integrate these three dimensions to understand the interactions and feedbacks among them. While this focus complements several core NSF programs, it differs by requiring that multiple dimensions of biodiversity be addressed simultaneously, to understand the roles of biodiversity in critical ecological and evolutionary processes.
Dimensions of Biodiversity
largerWorkCitation
program
Ocean Carbon and Biogeochemistry
http://us-ocb.org/
Ocean Carbon and Biogeochemistry
The Ocean Carbon and Biogeochemistry (OCB) program focuses on the ocean's role as a component of the global Earth system, bringing together research in geochemistry, ocean physics, and ecology that inform on and advance our understanding of ocean biogeochemistry. The overall program goals are to promote, plan, and coordinate collaborative, multidisciplinary research opportunities within the U.S. research community and with international partners. Important OCB-related activities currently include: the Ocean Carbon and Climate Change (OCCC) and the North American Carbon Program (NACP); U.S. contributions to IMBER, SOLAS, CARBOOCEAN; and numerous U.S. single-investigator and medium-size research projects funded by U.S. federal agencies including NASA, NOAA, and NSF.
The scientific mission of OCB is to study the evolving role of the ocean in the global carbon cycle, in the face of environmental variability and change through studies of marine biogeochemical cycles and associated ecosystems.
The overarching OCB science themes include improved understanding and prediction of: 1) oceanic uptake and release of atmospheric CO2 and other greenhouse gases and 2) environmental sensitivities of biogeochemical cycles, marine ecosystems, and interactions between the two.
The OCB Research Priorities (updated January 2012) include: ocean acidification; terrestrial/coastal carbon fluxes and exchanges; climate sensitivities of and change in ecosystem structure and associated impacts on biogeochemical cycles; mesopelagic ecological and biogeochemical interactions; benthic-pelagic feedbacks on biogeochemical cycles; ocean carbon uptake and storage; and expanding low-oxygen conditions in the coastal and open oceans.
OCB
largerWorkCitation
program
Biological Controls on the Ocean C:N:P ratios
https://osprey.bco-dmo.org/project/2178
Biological Controls on the Ocean C:N:P ratios
<p>One of the fundamental patterns of ocean biogeochemistry is the Redfield ratio, linking the stoichiometry of surface plankton with the chemistry of the deep ocean. There is no obvious mechanism for the globally consistent C:N:P ratio of 106:16:1 (Redfield ratio), especially as there is substantial elemental variation among plankton communities in different ocean regions. Thus, knowing how biodiversity regulates the elemental composition of the ocean is important for understanding the ocean and climate as a whole -- now and in the future.</p>
<p>The conceptual hypotheses for this study are as follows: 1. The C:N:P ratio of a cell is constrained by its broad taxonomic group, which determines, for example, whether it has an outer shell, its size, functional metabolism, membrane lipid composition. 2. Within a taxon, there is high genetic diversity. Some of this genetic diversity is potentially laterally transferred, or can be lost within taxa, and confers various functional abilities (organic phosphate assimilation, nitrate assimilation, photoheterotrophy, etc.). Functional diversity provides the cell with further flexibility, such as the ability to respond to varying nutrient supply rates/ratios, and affects a cell's C:N:P ratio within the range specified by the taxon. 3. Given these taxonomic and genetic constraints, a cell is physiologically plastic and modifies how it allocates cellular resources in response to nutrient supply rates/ratios in the environment. 4. The microbial diversity (taxonomic, genetic, and functional) of the surface ocean varies over time and space, driven by many factors in addition to nutrients. The sum of this mixture composes the ecosystem C:N:P, the ratio that Redfield described.</p>
<p>Based on this framework, the CoPIs will make field observations of taxon-specific stoichiometry and growth rates, genomic analyses, and conduct laboratory chemostat experiments to improve understanding of how ocean taxonomic, genetic, and functional biodiversity control the stoichiometry of the surface ocean plankton. Their analyses of these data would lead to a mechanistic understanding of variations in the Redfield ratio, both spatially and temporally.</p>
<p>This study will greatly expand knowledge of the genomic diversity among ocean microbes and how this diversity affects biogeochemistry. The stoichiometry of the ocean's microbes is a parameter that nearly every chemical or biological oceanographer uses, from converting measurements made in one element to another, to estimating regional and global nitrogen budgets. The research also has important implications for the global carbon budget and any changes that might result from climate change.</p>
<p>To understand mechanistically temporal and spatial variability of the plankton C:N:P ratio, biodiversity must be studied not only at the traditional taxonomic level, but at the genetic and functional levels which dictate organism response to their environment. Data will be integrated into a combined ocean ecological, evolutionary, and biogeochemical model, with flexible stoichiometry, including cellular biochemical allocations. Seeding a coupled physical-biological model of the oceans with multiple competing genotypes enables the exploration of ecological and evolutionary patterns of resource acquisition and C:N:P ratios. Developing a more mechanistic examination of the course of ecology and evolution, in which laboratory and field data define tradeoffs between different growth and nutrient acquisition strategies, would estabblish the framework of adaptive dynamics for determining "evolutionarily convergence". Finally, model outcomes will be evaluated against field data.</p>
<p>The field work planned for this project includes several cruises: BV46 (September/October 2011), BV48 (September 2012), a June 2013 cruise from Bermuda to the Labrador Sea, and a cruise from Hawaii to Tahiti (May 2014). Additionally, samples will be be acquired during cruises of opportunity.</p>
Biological C:N:P ratios
largerWorkCitation
project
eng; USA
oceans
NW Atlantic; Eastern Tropical North Pacific (ETNP) oxygen minimum zone off Manzanillo, Mexico; sampling transects run southeast-northwest along the Colima coast (~19-24°N, 106-116°W) and northeast-southwest off the coast (~14-18°N, 106-115°W); Hawaii; Hydrographic line IO9, Indian Ocean; Western Subtropical North Atlantic, ~32N - ~19N, ~65W; St. George's, Bermuda
-157
95
-20.76
55
2010-10-31
2016-04-15
western North Atlantic; 60N to 20N along 66W longitude; 20N to 15S in the tropical Pacific
0
BCO-DMO catalogue of parameters from Direct measure of phytoplankton cell quotas from field populations sampled from multiple cruises cruises between 2010 and 2016
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/850805.rdf
Name: Categorical_Region
Units: unitless
Description: <p>Ocean region name as defined in Lomas et al. 2021 publication (currently in revision)</p>
http://lod.bco-dmo.org/id/dataset-parameter/850806.rdf
Name: Cruise_ID
Units: unitless
Description: <p>Cruise identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/850807.rdf
Name: Date
Units: unitless
Description: <p>Date in format YYYY-MM-DD</p>
http://lod.bco-dmo.org/id/dataset-parameter/850808.rdf
Name: Latitude
Units: degrees North
Description: <p>Latitude of station sampled</p>
http://lod.bco-dmo.org/id/dataset-parameter/850809.rdf
Name: Longitude
Units: degrees West
Description: <p>Longitude of station sampled</p>
http://lod.bco-dmo.org/id/dataset-parameter/850810.rdf
Name: Station
Units: unitless
Description: <p>Station name on the specific cruise</p>
http://lod.bco-dmo.org/id/dataset-parameter/850811.rdf
Name: Depth
Units: meters
Description: <p>Depth of sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/850812.rdf
Name: Prochlorococcus_POC
Units: femtomole per cell (fmol/cell)
Description: <p>Particulate Organic Carbon (POC) per cell of in situ Prochlorococcus population, derived from flow cytometric sorting and subsequent chemical analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/850813.rdf
Name: Prochlorcoccus_PON
Units: femtomole per cell (fmol/cell)
Description: <p>Particulate Organic Nitrogen (PNC) per cell of in situ Prochlorococcus population, derived from flow cytometric sorting and subsequent chemical analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/850814.rdf
Name: Prochlorococcus_POP
Units: femtomole per cell (fmol/cell)
Description: <p>Particulate Organic Phosphorus (POP) per cell of in situ Prochlorococcus population, derived from flow cytometric sorting and subsequent chemical analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/850815.rdf
Name: Synechococcus_POC
Units: femtomole per cell (fmol/cell)
Description: <p>Particulate Organic Carbon (POC) per cell of in situ Synechococcus population, derived from flow cytometric sorting and subsequent chemical analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/850816.rdf
Name: Synechococcus_PON
Units: femtomole per cell (fmol/cell)
Description: <p>Particulate Organic Nitrogen (PNC) per cell of in situ Synechococcus population, derived from flow cytometric sorting and subsequent chemical analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/850817.rdf
Name: Synechococcus_POP
Units: femtomole per cell (fmol/cell)
Description: <p>Particulate Organic Phosphorus (POP) per cell of in situ Synechococcus population, derived from flow cytometric sorting and subsequent chemical analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/850818.rdf
Name: Eukaryote_POC
Units: femtomole per cell (fmol/cell)
Description: <p>Particulate Organic Carbon (POC) per cell of in situ eukaryote population, derived from flow cytometric sorting and subsequent chemical analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/850819.rdf
Name: Eukaryote_PON
Units: femtomole per cell (fmol/cell)
Description: <p>Particulate Organic Nitrogen (PNC) per cell of in situ eukaryote population, derived from flow cytometric sorting and subsequent chemical analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/850820.rdf
Name: Eukaryote_POP
Units: femtomole per cell (fmol/cell)
Description: <p>Particulate Organic Phosphorus (POP) per cell of in situ eukaryote population, derived from flow cytometric sorting and subsequent chemical analysis</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
10965
https://darchive.mblwhoilibrary.org/bitstream/1912/27189/1/dataset-849153_sorted-cell-quotas__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.849153.1
download
onLine
dataset
<p><em>Phytoplankton sample collection and preparation. </em>Samples for taxon-specific elemental content were collected using 12 liter (L) Niskin bottles, usually from two depths at each station (see supplemental file for map of stations from which samples were collected and cell-sorted to quantify cellular macronutrient content; one within the upper mixed layer (10-20 meters) and one near the deep chlorophyll maximum. Due to timing of the cruises, samples were collected from a seasonal window limited to summer and early fall. Samples were prepared for sorting by concentrating cells from the ambient sample under darkened conditions using a Memteq High Volume Cell Trap (0.2 micrometer pore size), and then flushed from the Cell Trap using 0.2 micrometer (µm) filtered seawater and a luer lock syringe; roughly 3-4 liters (L) of ambient seawater was concentrated down to ~5 milliliters (mls). Samples were either gravity filtered through the Cell Trap directly from the Niskin bottle or pushed through the cell trap using a peristaltic pump at &lt;50 milliliter per minute (ml min<sup>-1</sup>). Samples were then kept cool at 4 degrees C if they were going to be sorted live and immediately after collection or fixed with freshly filtered paraformaldehyde (0.5% v/v final concentration) if they were going to be stored for later sorting. Samples were allowed to fix at 4<sup> </sup>degrees C for 1-2 hours and then flash frozen in liquid nitrogen and then moved to -80<sup> </sup>degrees C for longer term storage.</p>
<p><em>Cell sorting and elemental analysis. </em>The cell counting and sorting protocols used follow that of Baer et al. (2017). Cell counts and sorts were performed on either a Becton Dickinson Influx or FACSJazz flow cytometer, each utilizing a 200 milliwatt (mW) 488 namometer (nm) laser, with detectors for forward scatter, side scatter, 692 namometer (nm), and 530 namometer (nm), and operated with 8 grams of NaCL kg<sup>-1</sup> solution for sheath fluid, which was filtered inline using a 0.22 micrometer (µm) Millipore Sterivex<sup>TM</sup> filter. Instrument alignment was performed with 3.0 micrometer (μm) 6-peak rainbow beads, while roughly hourly checks on forward scatter response were performed with 0.53 micrometer (μm) Nile Red beads (Spherotech). <em>Prochlorococcus</em> populations were discriminated based on forward scatter and red fluorescence, and a gate in orange (530 nm) discriminated for <em>Synechococcus</em>. Eukaryotes were all larger autofluorescing cells that did not fit the cyanobacterial gating scheme. For sorting, sort control software was set to "1.0 drop pure" sort mode. In excess of 17 million, 8 million, and 500,000 cells for <em>Prochlorococcus, Synechococcus, </em>and eukaryotes were sorted for chemical analysis, respectively. Not all phytoplankton groups were sufficiently abundant at each station/depth for practical sorting and subsequent analysis. Post-sort purity tests were run with subsamples of each sorted population; sort purity always exceeded 94%. Sorted populations were collected in polystyrene Falcon tubes (BD Biosciences Inc.) and subsequently filtered on pre-combusted (450 degrees C for 4 hours) GF-75 filters (Ahlstrom; nominal pore size = 0.3 micrometer). To ensure complete capture of all of the cells, the Falcon tubes were rinsed multiple times with 0.2 micrometer (μm) filtered sheath fluid. Filter and sheath fluid blanks were produced each day samples were sorted for subsequent subtraction from the mass of each unknown sample. Following filtration, filters were placed in acid-washed cryovials and frozen (-20 degrees C) until analysis as described below. Average cellular elemental content was determined by dividing the elemental content of the sample (see next section) by the number of sorted cells as determined in a direct post-sort count analysis of the sorted sample.</p>
<p><em>Particulate nutrients. </em>Particulate organic P (POP) was analyzed using the ash-hydrolysis method, with oxidation efficiency and standard recovery tested with each sample run using an ATP standard solution and a certified phosphate standard (OSIL Phosphate Nutrient Standard Solution). Method precision is 1-2% at 5 nanomoles per kilogram (nmol kg<sup>-1</sup>). Samples for POC and PON were acid fumed in a desiccator over concentrated HCl prior to analysis. After acid fuming, POC and PON were determined on a Costech 4010 elemental analyzer or a Control Equipment 440 elemental analyzer, depending on the samples. L-glutamic acid (USGS40) was used for standard curve generation and as a check standard (tolerance of &lt;0.1 microgram) approximately every ten samples and at the end of every instrument run. Empty tin capsules (Costech Analytical Technologies) were cleaned with acetone and dried and run as instrument blanks.</p>
<p>Additional methodological details can be found in Lomas et al. 2021 (currently in revision).</p>
Specified by the Principal Investigator(s)
<p>Data were calculated using standard Microsoft Excel.<br />
<br />
&nbsp;</p>
Specified by the Principal Investigator(s)
- Converted dates to ISO8601 date format (YYYY-MM-DD);
- Adjusted field/parameter names to comply with BCO-DMO naming conventions;
- Added a conventional header with dataset name, PI names, version date.
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Costech 4010 elemental analyzer, Control Equipment 440 elemental analyzer
Costech 4010 elemental analyzer, Control Equipment 440 elemental analyzer
PI Supplied Instrument Name: Costech 4010 elemental analyzer, Control Equipment 440 elemental analyzer PI Supplied Instrument Description:Costech 4010 elemental analyzer or a Control Equipment 440 elemental analyzer depending on the sample Instrument Name: CHN Elemental Analyzer Instrument Short Name:CHN_EA Instrument Description: A CHN Elemental Analyzer is used for the determination of carbon, hydrogen, and nitrogen content in organic and other types of materials, including solids, liquids, volatile, and viscous samples.
PI Supplied Instrument Name: PI Supplied Instrument Description:Becton Dickinson Influx or FACSJazz flow cytometer, each utilizing a 200 mW 488 nm laser, with detectors for forward scatter, side scatter, 692 nm, and 530 nm Instrument Name: Flow Cytometer Instrument Short Name:Flow Cytometer Instrument Description: Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/
PI Supplied Instrument Name: Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
Cruise: AE1319
AE1319
R/V Atlantic Explorer
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantic Explorer
vessel
AE1319
Michael W. Lomas
Bigelow Laboratory for Ocean Sciences
http://dmoserv3.whoi.edu/data_docs/Bio_CNP_Ratios/AE1319_Cruise_Report_09182013_reduced2.pdf
Report describing AE1319
Cruise: NH1410
NH1410
R/V New Horizon
Community Standard Description
International Council for the Exploration of the Sea
R/V New Horizon
vessel
NH1410
Frank James Stewart
Georgia Institute of Technology
http://dmoserv3.whoi.edu/data_docs/OMZ_SulfurCycling/Cruise_Report_NH1410.pdf
Report describing NH1410
Cruise: NH1418
NH1418
R/V New Horizon
Community Standard Description
International Council for the Exploration of the Sea
R/V New Horizon
vessel
NH1418
Michael W. Lomas
Bigelow Laboratory for Ocean Sciences
Cruise: RR1604
RR1604
R/V Roger Revelle
Community Standard Description
International Council for the Exploration of the Sea
R/V Roger Revelle
vessel
Cruise: SO243
SO243
R/V Sonne
Community Standard Description
R/V Sonne
vessel
Cruise: AE1032
AE1032
R/V Atlantic Explorer
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantic Explorer
vessel
AE1032
Michael W. Lomas
Bigelow Laboratory for Ocean Sciences
Cruise: AE1226
AE1226
R/V Atlantic Explorer
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantic Explorer
vessel
R/V Atlantic Explorer
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantic Explorer
vessel
R/V New Horizon
Community Standard Description
International Council for the Exploration of the Sea
R/V New Horizon
vessel
R/V Roger Revelle
Community Standard Description
International Council for the Exploration of the Sea
R/V Roger Revelle
vessel
R/V Sonne
Community Standard Description
R/V Sonne
vessel