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            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset/851142.rdf" xlink:actuate="onRequest">Phytoplankton growth and grazing mortality from HPLC pigments sampled in the Gulf of Mexico on R/V Nancy Foster cruises in May 2017 and May 2018</gmx:Anchor>
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            <gco:CharacterString>Cite this dataset as: Landry, M. R. (2021) Phytoplankton growth and grazing mortality from HPLC pigments sampled in the Gulf of Mexico on R/V Nancy Foster cruises in May 2017 and May 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-04-30 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.851142.1 [access date]</gco:CharacterString>
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        <gco:CharacterString>Phytoplankton growth and grazing mortality from HPLC pigments in the Gulf of Mexico Dataset Description:  Methods and Sampling: &amp;lt;p&amp;gt;This dataset is from CTD hydrocasts in the Gulf of Mexico from R/V Nancy Foster cruises in May 2017 and May 2018, which were part of a NOAA RESTORE project (aka: BLOOFINZ-GoM) to investigate the epipelagic marine nitrogen cycle, plankton dynamics, and impacts on growth and survival of larval Atlantic Bluefin Tuna (ABT). These data are meant to be used in inter-species, interregional comparisons to data from the BLOOFIN-IO study of larval Southern Bluefin Tuna in the Indian Ocean spawning region.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;On each cruise, we conducted multi-day quasi-Lagrangian experiments, called “cycles”, during which we sampled and measured processes on a repeated daily schedule following a satellite-tracked free-drifting array (Landry et al., 2009). The drift array (Pacific Gyre, San Diego) consisted of a surface float, a 3-m drogue centered at 15 m, coated-wire with stainless-steel attachment rings for in situ bottle incubations, and a separately attached smaller float with iridium transmission (10-min position frequency) and nighttime strobe light.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;For each experiment, we collected seawater daily from Niskin bottles on early-morning CTD hydrocasts (~02:00 local time) at 6 depths in the euphotic zone from 5 m to the deep chlorophyll maximum (DCM). Samples for initial concentrations of pigments, flow cytometry (FCM) and microscopy were filled directly from the Niskin bottles via silicone tubing. For each depth, we also prepared a dilution experiment that compared net population growth rates in polycarbonate bottles (2.7 L) containing unfiltered seawater (100%) and a dilution treatment consisting of ~32% whole seawater diluted with filtered seawater from that depth (Landry et al., 2008). Seawater was filtered directly from the Niskin bottles using a peristaltic pump, silicone tubing and an in-line 0.2 µm Suporcap filter capsule that had previously been acid washed (3.7% trace-metal grade HCl; Milli-Q and seawater rinses). Dilution bottles were first given a measured volume of filtered water and then gently filled to the top with unscreened water from the Niskin bottles.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;All bottles were secured into coarse net bags with top and bottom attachment clips and incubated in situ for 24 h at the depth of collection on the line below the drifter float. For the first deployment of each cycle, the entire array with bottles attached was laid out on deck before being quickly lowered by hand. For subsequent daily experiments, a new 6-depth experiment was set up in net bags on deck before recovering the drifter. The drifter was then recovered, the previous day’s experiments removed, the new experiments attached, and the drifter redeployed – a process that took ~15 min while the ship maintained position. All recovery and deployments were carried out before sunrise. Sampling for daily experiments was done in close proximity (~100 m) to the drifter position. Upon recovery, all bottles were subsampled for assessments of community composition and biomass, as described below.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Concentrations of chlorophyll and carotenoid pigments were determined using high-pressure liquid chromatography (HPLC) on 2.2-L samples filtered onto Whatman GF/F filters, frozen in liquid nitrogen and stored at -85°C. The samples were extracted and analyzed by the Horn Point Analytical Services Laboratory at the University of Maryland Center for Environmental Science using a C8 column and a reversed phase, methanol-based solvent protocol and an automated 1100 HPLC system with temperature-controlled autosampler, peltier temperature-controlled column oven compartment and PDA detector (Van Heukelem and Thomas, 2001; Hooker et al., 2012). Monovinyl and divinyl Chla were detected at 665 nm. Carotenoids and xanthophylls were detected at 450 nm. Concentrations were quantified from chromatograms relative to run standards using Agilent ChemStation software.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;We determined rate profiles for phytoplankton growth (µ, d-1) and microzooplankton grazing (m, d-1) from each pair of dilution experiment bottles and for each FCM or pigment-associated population according to the following equations:&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;m = (kd - k)/(1 - D) and µ = k + m,&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;where kd and k are the measured net rates of change between initial and final concentrations in the diluted and undiluted treatments, respectively, and D is the portion of unfiltered water in the dilution treatment (Landry et al., 2008; Selph et al., 2011).&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/819487.rdf" xlink:title="OCE-1851558" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1851558 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1851558</gmx:Anchor>
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The small area between NW Australia and Indonesia in the eastern Indian Ocean (IO) is the only known spawning ground of Southern Bluefin Tuna (SBT), a critically endangered top marine predator. Adult SBT migrate thousands of miles each year from high latitude feeding areas to lay their eggs in these tropical waters, where food concentrations on average are below levels that can support optimal feeding and growth of their larvae. Many critical aspects of this habitat are poorly known, such as the main source of nitrogen nutrient that sustains system productivity, how the planktonic food web operates to produce the unusual types of zooplankton prey that tuna larvae prefer, and how environmental differences in habitat quality associated with ocean fronts and eddies might be utilized by adult spawning tuna to give their larvae a greater chance for rapid growth and survival success. This project investigates these questions on a 38-day expedition in early 2021, during the peak time of SBT spawning. This project is a US contribution to the 2nd International Indian Ocean Expedition (IIOE-2) that advances understanding of biogeochemical and ecological dynamics in the poorly studied eastern IO. This is the first detailed study of nitrogen and carbon cycling in the region linking Pacific and IO waters. The shared dietary preferences of SBT larvae with those of other large tuna and billfish species may also make the insights gained broadly applicable to understanding larval recruitment issues for top consumers in other marine ecosystems. New information from the study will enhance international management efforts for SBT. The shared larval dietary preferences of large tuna and billfish species may also extend the insights gained broadly to many other marine top consumers, including Atlantic bluefin tuna that spawn in US waters of the Gulf of Mexico. The end-to-end study approach, highlights connections among physical environmental variability, biogeochemistry, and plankton food webs leading to charismatic and economically valuable fish production, is the theme for developing educational tools and modules through the &quot;scientists-in-the-schools&quot; program of the Center for Ocean-Atmospheric Prediction Studies at Florida State University, through a program for enhancing STEM learning pathways for underrepresented students in Hawaii, and through public outreach products for display at the Birch Aquarium in San Diego. The study also aims to support an immersive field experience to introduce talented high school students to marine research, with the goal of developing a sustainable marine-related educational program for underrepresented students in rural northwestern Florida.&lt;/p&gt;
&lt;p&gt;Southern Bluefin Tuna (SBT) migrate long distances from high-latitude feeding grounds to spawn exclusively in a small oligotrophic area of the tropical eastern Indian Ocean (IO) that is rich in mesoscale structures, driven by complex currents and seasonally reversing monsoonal winds. To survive, SBT larvae must feed and grow rapidly under environmental conditions that challenge conventional understanding of food-web structure and functional relationships in poor open-ocean systems. The preferred prey of SBT larvae, cladocerans and Corycaeidae copepods, are poorly studied and have widely different implications for trophic transfer efficiencies to larvae. Differences in nitrogen sources - N fixation vs deep nitrate of Pacific origin - to sustain new production in the region also has implications for conditions that may select for prey types (notably cladocerans) that enhance transfer efficiency and growth rates of SBT larvae. The relative importance of these N sources for the IO ecosystem may affect SBT resiliency to projected increased ocean stratification. This research expedition investigates how mesoscale variability in new production, food-web structure and trophic fluxes affects feeding and growth conditions for SBT larvae. Sampling across mesoscale features tests hypothesized relationships linking variability in SBT larval feeding and prey preferences (gut contents), growth rates (otolith analyses) and trophic positions (TP) to the environmental conditions of waters selected by adult spawners. Trophic Positions of larvae and their prey are determined using Compound-Specific Isotope Analyses of Amino Acids (CSIA-AA). Lagrangian experiments investigate underlying process rates and relationships through measurements of water-column 14C productivity, N2 fixation, 15NO3- uptake and nitrification; community biomass and composition (flow cytometry, pigments, microscopy, in situ imaging, genetic analyses); and trophic fluxes through micro- and mesozooplankton grazing, remineralization and export. Biogeochemical and food web elements of the study are linked by CSIA-AA (N source, TP), 15N-constrained budgets and modeling. The project elements comprise an end-to-end coupled biogeochemistry-trophic study as has not been done previously for any pelagic ecosystem.&lt;/p&gt;
&lt;p&gt;This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.&lt;/p&gt;</gco:CharacterString>
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&amp;lt;p&amp;gt;On each cruise, we conducted multi-day quasi-Lagrangian experiments, called “cycles”, during which we sampled and measured processes on a repeated daily schedule following a satellite-tracked free-drifting array (Landry et al., 2009). The drift array (Pacific Gyre, San Diego) consisted of a surface float, a 3-m drogue centered at 15 m, coated-wire with stainless-steel attachment rings for in situ bottle incubations, and a separately attached smaller float with iridium transmission (10-min position frequency) and nighttime strobe light.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;For each experiment, we collected seawater daily from Niskin bottles on early-morning CTD hydrocasts (~02:00 local time) at 6 depths in the euphotic zone from 5 m to the deep chlorophyll maximum (DCM). Samples for initial concentrations of pigments, flow cytometry (FCM) and microscopy were filled directly from the Niskin bottles via silicone tubing. For each depth, we also prepared a dilution experiment that compared net population growth rates in polycarbonate bottles (2.7 L) containing unfiltered seawater (100%) and a dilution treatment consisting of ~32% whole seawater diluted with filtered seawater from that depth (Landry et al., 2008). Seawater was filtered directly from the Niskin bottles using a peristaltic pump, silicone tubing and an in-line 0.2 µm Suporcap filter capsule that had previously been acid washed (3.7% trace-metal grade HCl; Milli-Q and seawater rinses). Dilution bottles were first given a measured volume of filtered water and then gently filled to the top with unscreened water from the Niskin bottles.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;All bottles were secured into coarse net bags with top and bottom attachment clips and incubated in situ for 24 h at the depth of collection on the line below the drifter float. For the first deployment of each cycle, the entire array with bottles attached was laid out on deck before being quickly lowered by hand. For subsequent daily experiments, a new 6-depth experiment was set up in net bags on deck before recovering the drifter. The drifter was then recovered, the previous day’s experiments removed, the new experiments attached, and the drifter redeployed – a process that took ~15 min while the ship maintained position. All recovery and deployments were carried out before sunrise. Sampling for daily experiments was done in close proximity (~100 m) to the drifter position. Upon recovery, all bottles were subsampled for assessments of community composition and biomass, as described below.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Concentrations of chlorophyll and carotenoid pigments were determined using high-pressure liquid chromatography (HPLC) on 2.2-L samples filtered onto Whatman GF/F filters, frozen in liquid nitrogen and stored at -85°C. The samples were extracted and analyzed by the Horn Point Analytical Services Laboratory at the University of Maryland Center for Environmental Science using a C8 column and a reversed phase, methanol-based solvent protocol and an automated 1100 HPLC system with temperature-controlled autosampler, peltier temperature-controlled column oven compartment and PDA detector (Van Heukelem and Thomas, 2001; Hooker et al., 2012). Monovinyl and divinyl Chla were detected at 665 nm. Carotenoids and xanthophylls were detected at 450 nm. Concentrations were quantified from chromatograms relative to run standards using Agilent ChemStation software.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;We determined rate profiles for phytoplankton growth (µ, d-1) and microzooplankton grazing (m, d-1) from each pair of dilution experiment bottles and for each FCM or pigment-associated population according to the following equations:&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;m = (kd - k)/(1 - D) and µ = k + m,&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;where kd and k are the measured net rates of change between initial and final concentrations in the diluted and undiluted treatments, respectively, and D is the portion of unfiltered water in the dilution treatment (Landry et al., 2008; Selph et al., 2011).&amp;lt;/p&amp;gt;</gco:CharacterString>
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This term applies to profiling CTDs. For fixed CTDs, see https://www.bco-dmo.org/instrument/869934. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/130/</gco:CharacterString>
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            <gco:CharacterString>PI Supplied Instrument Name: drift array PI Supplied Instrument Description:The drift array (Pacific Gyre, San Diego) consisted of a surface float, a 3-m drogue centered at 15 m, coated-wire with stainless-steel attachment rings for in situ bottle incubations, and a separately attached smaller float with iridium transmission (10-min position frequency) and nighttime strobe light. Instrument Name: Drifter Buoy Instrument Short Name:   Instrument Description: Drifting buoys are free drifting platforms with a float or buoy that keep the drifter at the surface and underwater sails or socks that catch the current. These instruments sit at the surface of the ocean and are transported via near-surface ocean currents. They are not fixed to the ocean bottom, therefore they &quot;drift&quot; with the currents. For this reason, these instruments are referred to as drifters, or drifting buoys.

The surface float contains sensors that measure different parameters, such as sea surface temperature, barometric pressure, salinity, wave height, etc. Data collected from these sensors are transmitted to satellites passing overhead, which are then relayed to land-based data centers.

definition sources: https://mmisw.org/ont/ioos/platform/drifting_buoy and https://www.aoml.noaa.gov/phod/gdp/faq.php#drifter1</gco:CharacterString>
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                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/506.rdf" xlink:title="High-Performance Liquid Chromatograph" xlink:actuate="onRequest">Agilent 1100 HPLC system</gmx:Anchor>
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            <gco:CharacterString>Agilent 1100 HPLC system</gco:CharacterString>
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            <gco:CharacterString>PI Supplied Instrument Name: Agilent 1100 HPLC system PI Supplied Instrument Description:Agilent 1100 HPLC system with temperature-controlled autosampler, Peltier temperature-controlled column oven compartment and PDA detector. Instrument Name: High-Performance Liquid Chromatograph Instrument Short Name:HPLC   Instrument Description: A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB11/</gco:CharacterString>
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            <gco:CharacterString>PI Supplied Instrument Name: Niskin bottles Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle   Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/</gco:CharacterString>
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            <gmi:parentOperation gco:nilReason="inapplicable"/>
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            <gmi:parentOperation gco:nilReason="inapplicable"/>
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