http://lod.bco-dmo.org/id/dataset/852869
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2021-06-02
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Neocalanus copepod egg production is described from the Northern Gulf of Alaska for Fall 2015, 2016, 2017
2021-11-08
publication
2021-11-08
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2021-11-08
publication
https://doi.org/10.26008/1912/bco-dmo.852869.1
Russell R. Hopcroft
University of Alaska Fairbanks
principalInvestigator
Petra H. Lenz
University of Hawaiʻi at Mānoa
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Hopcroft, R. R., Lenz, P. (2021) Neocalanus copepod egg production is described from the Northern Gulf of Alaska for Fall 2015, 2016, 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-11-08 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.852869.1 [access date]
Copepod Egg Production Dataset Description: Methods and Sampling: <p>Neocalanus flemingeri females were collected in September of 2015, 2016 and 2017 from Knight Island Passage in Prince William Sound, Northern Gulf of Alaska using a Midi MultiNet equipped with a 150 um mesh. Healthy females, taken from samples collected below 400m, were placed individually in 500 ml tissue culture flasks.&nbsp;</p>
<p>Flasks were filled with seawater from Niskin bottles taken at the same depth as female collection, and sieved through a 35um mesh to remove metazoans.&nbsp; The flasks were maintained in darkened incubators at a temperature of 5 degrees Celsius. The animals were checked every 2-3 days for the presence of eggs or nauplli.</p>
<p>If production was observed, the female was transferred to a new flask and the eggs and nauplii were sieved from the container and counted under the microscope.&nbsp; This procedure was continued until all of the females either died or were spawned out (i.e. no ovaries or lipid storage were still present).</p>
<p>For 2015, and 2016 the prosome length of the female was measured after the egg production had ceased. However this length may have not been representative if the females were diseased or dead.&nbsp; Thus, for 2017 the female was photographed just before she was placed in the flask and the prosome length was measured directly from the scaled image.</p>
<p>Location of Animal Collection per cruise (station KIP2):</p>
<p>TXF15</p>
<ul>
<li>Latitude (decimal degrees): 60.275</li>
<li>Longitude (decimal degrees): -147.987</li>
</ul>
<p>TXF16</p>
<ul>
<li>Latitude (decimal degrees): 60.2752</li>
<li>Longitude (decimal degrees): -147.989</li>
</ul>
<p>TXF17</p>
<ul>
<li>Latitude (decimal degrees): 60.7313</li>
<li>Longitude (decimal degrees): -147.987</li>
</ul>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1459826 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1459826
completed
Russell R. Hopcroft
University of Alaska Fairbanks
907-474-7842
College of Fisheries and Ocean Sciences, Institute of Marine Sciences 120 O'Neill
Fairbanks
AK
99775-7220
USA
rrhopcroft@alaska.edu
pointOfContact
Petra H. Lenz
University of Hawaiʻi at Mānoa
808-956-8003
Pacific Biosciences Research Center (PBRC), Bekesy Laboratory of Neurobiology 1993 East-West Road
Honolulu
HI
96822
United States
petra@hawaii.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Year
Animal
Prosome_length
Consecutive_Clutch
Date_of_observation
Consecutive_day
Eggs
Nauplii
Midi Multinet
theme
None, User defined
year
individual
length
number
date
day of month
featureType
BCO-DMO Standard Parameters
MultiNet
instrument
BCO-DMO Standard Instruments
TXF15
TXF16
TXF17
service
Deployment Activity
Northern Gulf of Alaska
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Proposal: Optimizing Recruitment of Neocalanus copepods through Strategic Timing of Reproduction and Growth in the Gulf of Alaska
https://osprey.bco-dmo.org/project/542182
Collaborative Proposal: Optimizing Recruitment of Neocalanus copepods through Strategic Timing of Reproduction and Growth in the Gulf of Alaska
<p>NSF abstract:</p>
<p>The Gulf of Alaska supports a diverse and productive marine community that includes many commercially important fishes. Toward the base of this food web are small planktonic crustaceans that serve as the primary food source for many of these fish, as well as seabirds and marine mammals. The copepod Neocalanus flemingeri is one of these crustaceans, and it experiences rapid population growth during each spring's algal, or phytoplankton, bloom. An apparent mismatch between the presence of the youngest stages of the copepod, or nauplii, in early winter and the unpredictable timing of the spring phytoplankton bloom several months later raises important questions about when females reproduce and how this relates to survival and growth of nauplii. Two types of dormancy, diapause in adult females and physiological quiescence in nauplii, may be the key to the success of this copepod species. Timing and duration of the egg-laying period by adult females is linked to emergence from diapause. In addition, nauplii may enter a state of physiological quiescence while food resources are low, resuming growth after phytoplankton levels increase. This research will address a long-standing goal of biological oceanographers to understand dormancy and its role in controlling population cycles in marine copepods. It will use new technologies in molecular biology called transcriptomics to catalog the messages used by the cells to control copepod life processes, in this case those related to dormancy in adults and nauplii. Undergraduate students and a postdoctoral investigator will be trained in interdisciplinary research, and students from Native Hawaiian and Native Alaskan groups will be targeted for participation. Fishing is a major industry in the Gulf of Alaska, and outreach will focus on communicating the role copepods play in marine ecosystems. New content, including images, will be generated for existing websites: the Seward Line long-term observation program, the Alaska Ocean Observing System and the Gulf Watch Alaska Program.</p>
<p>Recruitment to the Neocalanus flemingeri spring population is dependent on successful emergence from diapause followed by reproduction, survival, and growth of the next generation. Individual-based models have made significant progress in predicting population growth in calanoid copepods using food, temperature, and advection as key environmental factors. Few of these models include predictors for naupliar recruitment, however, because little is known about this part of the life cycle given sampling difficulties and the lack of biomarkers to evaluate physiological state. This study will leverage existing monitoring efforts to track the N. flemingeri population during the winter and early spring. The research team will combine laboratory and field approaches to determine duration and synchronization of reproduction in emerging females and strategies for naupliar survival during low food conditions. Zooplankton samples will be processed to enumerate nauplii to species and to determine physiological condition of both nauplii and adult females. Gene expression studies will develop molecular markers for female dormancy and reproductive readiness and for naupliar growth and possible dormancy, which in turn will be used to evaluate field collected individuals. This will be the first comprehensive study to combine molecular and traditional tools to connect diapausing adults, naupliar production, and the resulting spring population of copepodites.</p>
Neocalanus Gulf of Alaska
largerWorkCitation
project
eng; USA
oceans
Northern Gulf of Alaska
-147.989
-147.987
60.275
60.7313
2015-09-18
2017-09-21
Gulf of Alaska; Seward Line
0
BCO-DMO catalogue of parameters from Neocalanus copepod egg production is described from the Northern Gulf of Alaska for Fall 2015, 2016, 2017
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/852888.rdf
Name: Year
Units: unitless
Description: <p>The year of animal collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/852889.rdf
Name: Animal
Units: unitless
Description: <p>The individual animal identification number within each year</p>
http://lod.bco-dmo.org/id/dataset-parameter/852890.rdf
Name: Prosome_length
Units: micrometer (um)
Description: <p>The prosome length of the individual animal</p>
http://lod.bco-dmo.org/id/dataset-parameter/852891.rdf
Name: Consecutive_Clutch
Units: unitless
Description: <p>The consecutive clutch number for each individual animal</p>
http://lod.bco-dmo.org/id/dataset-parameter/852892.rdf
Name: Date_of_observation
Units: unitless
Description: <p>The date that egg production was observed</p>
http://lod.bco-dmo.org/id/dataset-parameter/852893.rdf
Name: Consecutive_day
Units: unitless
Description: <p>The consecutive day from animal collection when activity was observed</p>
http://lod.bco-dmo.org/id/dataset-parameter/852894.rdf
Name: Eggs
Units: unitless
Description: <p>The number of eggs produced per given clutch</p>
http://lod.bco-dmo.org/id/dataset-parameter/852895.rdf
Name: Nauplii
Units: unitless
Description: <p>The number of nauplii observed per given clutch</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
19971
https://darchive.mblwhoilibrary.org/bitstream/1912/27723/1/dataset-852869_neocalanus-egg-production__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.852869.1
download
onLine
dataset
<p>Neocalanus flemingeri females were collected in September of 2015, 2016 and 2017 from Knight Island Passage in Prince William Sound, Northern Gulf of Alaska using a Midi MultiNet equipped with a 150 um mesh. Healthy females, taken from samples collected below 400m, were placed individually in 500 ml tissue culture flasks.&nbsp;</p>
<p>Flasks were filled with seawater from Niskin bottles taken at the same depth as female collection, and sieved through a 35um mesh to remove metazoans.&nbsp; The flasks were maintained in darkened incubators at a temperature of 5 degrees Celsius. The animals were checked every 2-3 days for the presence of eggs or nauplli.</p>
<p>If production was observed, the female was transferred to a new flask and the eggs and nauplii were sieved from the container and counted under the microscope.&nbsp; This procedure was continued until all of the females either died or were spawned out (i.e. no ovaries or lipid storage were still present).</p>
<p>For 2015, and 2016 the prosome length of the female was measured after the egg production had ceased. However this length may have not been representative if the females were diseased or dead.&nbsp; Thus, for 2017 the female was photographed just before she was placed in the flask and the prosome length was measured directly from the scaled image.</p>
<p>Location of Animal Collection per cruise (station KIP2):</p>
<p>TXF15</p>
<ul>
<li>Latitude (decimal degrees): 60.275</li>
<li>Longitude (decimal degrees): -147.987</li>
</ul>
<p>TXF16</p>
<ul>
<li>Latitude (decimal degrees): 60.2752</li>
<li>Longitude (decimal degrees): -147.989</li>
</ul>
<p>TXF17</p>
<ul>
<li>Latitude (decimal degrees): 60.7313</li>
<li>Longitude (decimal degrees): -147.987</li>
</ul>
Specified by the Principal Investigator(s)
<p>All egg and nauplii production were compiled into an EXCEL spreadsheet and the final flat-field output was consolidated from the original matrix</p>
<p>Any observations without egg production or nauplii presence were removed. The data from animals that showed disease throughout the experiment were also removed.</p>
<p>BCO-DMO Processing notes:</p>
<ul>
<li>No adjustments have been made</li>
</ul>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Midi Multinet
Midi Multinet
PI Supplied Instrument Name: Midi Multinet PI Supplied Instrument Description:Midi Multinet (0.25 m2) equipped with 150 um mesh
Instrument Name: MultiNet Instrument Short Name:MultiNet Instrument Description: The MultiNet© Multiple Plankton Sampler is designed as a sampling system for horizontal and vertical collections in successive water layers. Equipped with 5 or 9 net bags, the MultiNet© can be delivered in 3 sizes (apertures) : Mini (0.125 m2), Midi (0.25 m2) and Maxi (0.5 m2). The system consists of a shipboard Deck Command Unit and a stainless steel frame to which 5 (or 9) net bags are attached by means of zippers to canvas. The net bags are opened and closed by means of an arrangement of levers that are triggered by a battery powered Motor Unit. The commands for actuation of the net bags are given via single or multi-conductor cable between the Underwater Unit and the Deck Command Unit. Although horizontal collections typically use a mesh size of 300 microns, mesh sizes from 100 to 500 may also be used. Vertical collections are also common. The shipboard Deck Command Unit displays all relevant system data, including the actual operating depth of the net system. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/NETT0099/
Cruise: TXF15
TXF15
R/V Tiglax
Community Standard Description
International Council for the Exploration of the Sea
R/V Tiglax
vessel
TXF15
Russell R. Hopcroft
University of Alaska Fairbanks
https://www.ncei.noaa.gov/access/ocean-carbon-acidification-data-system/oceans/Coastal/seward.html
Report describing TXF15
Cruise: TXF16
TXF16
R/V Tiglax
Community Standard Description
International Council for the Exploration of the Sea
R/V Tiglax
vessel
TXF16
Russell R. Hopcroft
University of Alaska Fairbanks
https://www.ncei.noaa.gov/access/ocean-carbon-acidification-data-system/oceans/Coastal/seward.html
Report describing TXF16
Cruise: TXF17
TXF17
R/V Tiglax
Community Standard Description
International Council for the Exploration of the Sea
R/V Tiglax
vessel
TXF17
Russell R. Hopcroft
University of Alaska Fairbanks
https://www.ncei.noaa.gov/access/ocean-carbon-acidification-data-system/oceans/Coastal/seward.html
Report describing TXF17
R/V Tiglax
Community Standard Description
International Council for the Exploration of the Sea
R/V Tiglax
vessel