http://lod.bco-dmo.org/id/dataset/854424
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2021-06-23
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Sample metadata for MOCNESS tows conducted on R/V Kilo Moana cruise KM1910 at Station ALOHA during June 2019
2021-05-25
publication
2021-05-25
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2022-02-15
publication
https://doi.org/10.26008/1912/bco-dmo.854424.1
Erica Goetze
University of Hawaiʻi at Mānoa
principalInvestigator
Matthew J. Church
University of Montana
principalInvestigator
Sara Ferrón
University of Hawaiʻi at Mānoa
principalInvestigator
Angelicque E. White
University of Hawaiʻi at Mānoa
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Goetze, E., Church, M. J., White, A. E., Ferron, S. (2022) Sample metadata for MOCNESS tows conducted on R/V Kilo Moana cruise KM1910 at Station ALOHA during June 2019. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-05-25 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.854424.1 [access date]
Sample Metadata for MOCNESS Tows Dataset Description: <p>Data have been published “as is”. Final review by the data submitter was not received after it was imported into the BCO-DMO data system.</p> Methods and Sampling: <p>The zooplankton assemblage was collected using a 1m<sup>2</sup> Multiple Opening/Closing Nets and Environmental Sampling System (MOCNESS), with all nets and cod ends at 202 µm mesh. All tows were conducted at Station ALOHA (22° 45’N 158° W) A total of 5 tows sampled the full water column between 1000 m and the sea surface, with 3 day and 2 night paired tows. Depth strata were as follows: 0-1000m (Net 0), 1000-800 m (Net 1), 800-600m (Net 2), 600-500m (Net 3), 500-400m (Net 4), 400-300m (Net 5), 300-200m (Net 6), 200-100m (Net 7), 100-50m (Net 8), 50-0m (Net 9).</p>
<p>Bulk plankton from each net and tow were quantitatively split using a Folsom plankton splitter, with additional processing or preservation for several analyses.&nbsp; Two additional ‘short’ MOCNESS tows were conducted to sample the base of the mesopelagic (700-1000m) and epipelagic (0-200 m) for metatranscriptomic analyses of the zooplankton assemblage.&nbsp; Rapid recovery and preservation of the material was important in this case, given primary interest in gene expression profiles, and so the nets were flown as quickly as possible through midwater.</p>
<p>Quantitative subsamples of bulk plankton were preserved as follows:</p>
<ol>
<li>Formalin-preserved – sodium tetraborate buffered formaldehyde, preserved broadly following CalCOFI protocols (<a href="https://calcofi.org/about-calcofi/calcofi-handbook/301-net-sample-preservation.html">https://calcofi.org/about-calcofi/calcofi-handbook/301-net-sample-preservation.html</a>).&nbsp; Analyses: ZooScan, Microscopy</li>
<li>Size-fractionated into 5 or 2 fractions (0.2-0.5 mm, 0.5-1.0 mm, 1.0-2.0 mm, 2.0-5.0 mm, &gt;5.0 mm OR 0.2-1.0 mm, &gt;1.0 mm), filtered onto 200 µm Nitex filters, rinsed with ammonium formate, and followed by cryopreservation and storage at -80° C. Analyses: biomass, bioelements, isotopes.</li>
<li>Filtered onto 200 µm Nitex filters, followed by preservation in RNALater and storage at -80° C. Analyses: Metabarcoding.</li>
<li>Filtered onto 200 µm Nitex filters, flash frozen and stored in liquid nitrogen. Analyses: Electron Transport System (ETS) activity assay.</li>
<li>Size-fractionated into 2 fractions (0.2 - 1.0 mm, &gt; 1.0 mm) and analyzed shipboard for alkaline phosphatase activity (APA assay)</li>
</ol>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1911831 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1911831
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1911990 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1911990
completed
Erica Goetze
University of Hawaiʻi at Mānoa
808-956-7156
Department of Oceanography, University of Hawaii at Manoa 1000 Pope Road
Honolulu
HI
96822
United States
egoetze@hawaii.edu
pointOfContact
Matthew J. Church
University of Montana
(406) 982-3301
Flathead Lake Biological Station, University of Montana 321225 Bio Station Lane
Polson
MT
59860-6815
USA
matt.church@flbs.umt.edu
pointOfContact
Sara Ferrón
University of Hawaiʻi at Mānoa
(808) 956-3420
1950 East West Road
Honolulu
HI
96822
USA
sferron@hawaii.edu
pointOfContact
Angelicque E. White
University of Hawaiʻi at Mānoa
808-956-6220
1950 East West Rd.
Honolulu
HI
USA
aewhite@hawaii.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
ID
Event_no
Tow_no
Latitude_decimal_degrees
Longitude_decimal_degrees
Time_HST
Time_UTC
Day_vs_night_tow
Net_no
Target_depths_m
Target_depth_min_m
Target_depth_max_m
Bulk_fraction
Size_fraction
Size_fraction_min
Size_fraction_max
Purpose_analysis
Preservation
Folsom Plankton Splitter
1m2 MOCNESS
theme
None, User defined
sample identification
event
tow
latitude
longitude
time_local
time_utc
time_of_day
net
depth
depth_min
depth_max
sample description
comments
featureType
BCO-DMO Standard Parameters
Folsom Plankton Splitter
MOCNESS1
instrument
BCO-DMO Standard Instruments
KM1910
service
Deployment Activity
Honolulu, Hawai'i
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
EAGER Collaborative Research: Early career chief scientist training for biological and chemical oceanographers
https://osprey.bco-dmo.org/project/805876
EAGER Collaborative Research: Early career chief scientist training for biological and chemical oceanographers
<p><em>NSF Award Abstract:</em></p>
<p>Intellectual Merit<br />
The PIs request funds to provide training in leading and organizing research cruises to early career researchers in the areas of Biological and Chemical Oceanography. Participants in this training program would be introduced to pre-cruise planning and logistics, receive training in commonly used oceanographic sampling equipment, and conduct shipboard measurements during a 10-day oceanographic cruise to the North Pacific Subtropical Gyre (NPSG). The goal of this training program is to prepare early career scientists for leading and participating in interdisciplinary oceanographic research at sea.</p>
<p>Broader Impacts<br />
The proposed program addresses the broader impacts criteria successfully. The research cruise and follow-up reports and publications focus on interdisciplinary questions important for advancing the field. Given the rapid changes that oceanic systems are undergoing, it is important to have a cadre of junior scientists who are adept at managing interdisciplinary collaborations and conducting research at sea. The PIs are considering ways to connect with diverse audiences in recruiting participants. The impact on early career oceanographers will be very strong. This will create an experience that will be a major impact on the careers of the trainees, especially if they stay in the oceanography field.</p>
Chief Sci KM1910
largerWorkCitation
project
eng; USA
oceans
Honolulu, Hawai'i
-158.081
-157.955
22.6879
22.8433
2019-06-01
2019-06-30
Station ALOHA (22.75N, 158W), North Pacific Ocean
0
BCO-DMO catalogue of parameters from Sample metadata for MOCNESS tows conducted on R/V Kilo Moana cruise KM1910 at Station ALOHA during June 2019
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/854850.rdf
Name: ID
Units: unitless
Description: <p>Unique record idenfitier</p>
http://lod.bco-dmo.org/id/dataset-parameter/854851.rdf
Name: Event_no
Units: unitless
Description: <p>Event identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/854852.rdf
Name: Tow_no
Units: unitless
Description: <p>Tow identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/854853.rdf
Name: Latitude_decimal_degrees
Units: decimal degrees
Description: <p>Latitude (degrees N)</p>
http://lod.bco-dmo.org/id/dataset-parameter/854854.rdf
Name: Longitude_decimal_degrees
Units: decimal degrees
Description: <p>Longitude (degrees E)</p>
http://lod.bco-dmo.org/id/dataset-parameter/854855.rdf
Name: Time_HST
Units: unitless
Description: <p>Local time</p>
http://lod.bco-dmo.org/id/dataset-parameter/854856.rdf
Name: Time_UTC
Units: unitless
Description: <p>Time in UTC</p>
http://lod.bco-dmo.org/id/dataset-parameter/854857.rdf
Name: Day_vs_night_tow
Units: unitless
Description: <p>Time of day when a zooplankton sample was taken. "N" indicates night, and "D" indicates a daytime sample. "N short" and "D short" correspond to 2 abbreviated MOCNESS tows conducted to sample zooplankton for metatranscriptomics in the epipelagic and deep mesopelagic.</p>
http://lod.bco-dmo.org/id/dataset-parameter/854858.rdf
Name: Net_no
Units: unitless
Description: <p>Net identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/854859.rdf
Name: Target_depths_m
Units: meters (m)
Description: <p>Target collection depth range</p>
http://lod.bco-dmo.org/id/dataset-parameter/854860.rdf
Name: Target_depth_min_m
Units: meters (m)
Description: <p>Target depth minimum</p>
http://lod.bco-dmo.org/id/dataset-parameter/854861.rdf
Name: Target_depth_max_m
Units: meters (m)
Description: <p>Target depth maximum</p>
http://lod.bco-dmo.org/id/dataset-parameter/854862.rdf
Name: Bulk_fraction
Units: unitless
Description: <p>Bulk fraction is the quantitative subsample of the original bulk plankton from that tow/net.</p>
http://lod.bco-dmo.org/id/dataset-parameter/854863.rdf
Name: Size_fraction
Units: milimeters (mm)
Description: <p>Size fractions as follows: NSF = not size-fractionated, 0.2-1.0mm [200 um - 1000 um], > 1.0mm [ > 1000 um].</p>
http://lod.bco-dmo.org/id/dataset-parameter/854864.rdf
Name: Size_fraction_min
Units: milimeters (mm)
Description: <p>Size fraction minimums as follows: NSF = not size-fractionated, 0.2-1.0mm [200 um - 1000 um], > 1.0mm [ > 1000 um].</p>
http://lod.bco-dmo.org/id/dataset-parameter/854865.rdf
Name: Size_fraction_max
Units: milimeters (mm)
Description: <p>Size fractions maximum as follows: NSF = not size-fractionated, 0.2-1.0mm [200 um - 1000 um], > 1.0mm [ > 1000 um].</p>
http://lod.bco-dmo.org/id/dataset-parameter/854866.rdf
Name: Purpose_analysis
Units: unitless
Description: <p>Purpose of collection and subsiquent analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/854867.rdf
Name: Preservation
Units: unitless
Description: <p>Preservation method applied to collected plankton</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
51963
https://darchive.mblwhoilibrary.org/bitstream/1912/28005/1/dataset-854424_sample-metadata-mocness-tows__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.854424.1
download
onLine
dataset
<p>The zooplankton assemblage was collected using a 1m<sup>2</sup> Multiple Opening/Closing Nets and Environmental Sampling System (MOCNESS), with all nets and cod ends at 202 µm mesh. All tows were conducted at Station ALOHA (22° 45’N 158° W) A total of 5 tows sampled the full water column between 1000 m and the sea surface, with 3 day and 2 night paired tows. Depth strata were as follows: 0-1000m (Net 0), 1000-800 m (Net 1), 800-600m (Net 2), 600-500m (Net 3), 500-400m (Net 4), 400-300m (Net 5), 300-200m (Net 6), 200-100m (Net 7), 100-50m (Net 8), 50-0m (Net 9).</p>
<p>Bulk plankton from each net and tow were quantitatively split using a Folsom plankton splitter, with additional processing or preservation for several analyses.&nbsp; Two additional ‘short’ MOCNESS tows were conducted to sample the base of the mesopelagic (700-1000m) and epipelagic (0-200 m) for metatranscriptomic analyses of the zooplankton assemblage.&nbsp; Rapid recovery and preservation of the material was important in this case, given primary interest in gene expression profiles, and so the nets were flown as quickly as possible through midwater.</p>
<p>Quantitative subsamples of bulk plankton were preserved as follows:</p>
<ol>
<li>Formalin-preserved – sodium tetraborate buffered formaldehyde, preserved broadly following CalCOFI protocols (<a href="https://calcofi.org/about-calcofi/calcofi-handbook/301-net-sample-preservation.html">https://calcofi.org/about-calcofi/calcofi-handbook/301-net-sample-preservation.html</a>).&nbsp; Analyses: ZooScan, Microscopy</li>
<li>Size-fractionated into 5 or 2 fractions (0.2-0.5 mm, 0.5-1.0 mm, 1.0-2.0 mm, 2.0-5.0 mm, &gt;5.0 mm OR 0.2-1.0 mm, &gt;1.0 mm), filtered onto 200 µm Nitex filters, rinsed with ammonium formate, and followed by cryopreservation and storage at -80° C. Analyses: biomass, bioelements, isotopes.</li>
<li>Filtered onto 200 µm Nitex filters, followed by preservation in RNALater and storage at -80° C. Analyses: Metabarcoding.</li>
<li>Filtered onto 200 µm Nitex filters, flash frozen and stored in liquid nitrogen. Analyses: Electron Transport System (ETS) activity assay.</li>
<li>Size-fractionated into 2 fractions (0.2 - 1.0 mm, &gt; 1.0 mm) and analyzed shipboard for alkaline phosphatase activity (APA assay)</li>
</ol>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing:</strong></p>
<ul>
<li>renamed fields to remove spaces, and problematic characters</li>
<li>converted time to UTC</li>
<li>converted lat lon to decimal degrees&nbsp;</li>
<li>split size fraction column into min and max values</li>
<li>split target depths column into min and max values</li>
</ul>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Folsom Plankton Splitter
Folsom Plankton Splitter
PI Supplied Instrument Name: Folsom Plankton Splitter PI Supplied Instrument Description:Bulk plankton from each net and tow were quantitatively split using a Folsom plankton splitter, with additional processing or preservation for several analyses. Instrument Name: Folsom Plankton Splitter Instrument Short Name:Folsom Splitter Instrument Description: A device for sub-sampling of plankton and ichthyoplankton samples by splitting, developed by Dr. Folsom of the Scripps Institute of Oceanography. Ideally suited for splitting plankton samples with minimal debris. A measured volume of plankton sample is placed in the undivided section of the drum. This is rotated 120 degrees to divide the stirred sample with a separating blade. Standard Methods suggests splitting until a subsample of 200-500 individuals is obtained.
1m2 MOCNESS
1m2 MOCNESS
PI Supplied Instrument Name: 1m2 MOCNESS PI Supplied Instrument Description:The zooplankton assemblage was collected using a 1m2 Multiple Opening/Closing Nets and Environmental Sampling System (MOCNESS), with all nets and cod ends at 202 µm mesh. All tows were conducted at Station ALOHA (22° 45’N 158° W). A total of 5 tows sampled the full water column between 1000 m and the sea surface, with 3 day and 2 night paired tows. Depth strata were as follows: 0-1000m (Net 0), 1000-800 m (Net 1), 800-600m (Net 2), 600-500m (Net 3), 500-400m (Net 4), 400-300m (Net 5), 300-200m (Net 6), 200-100m (Net 7), 100-50m (Net 8), 50-0m (Net 9). Instrument Name: MOCNESS1 Instrument Short Name:MOC1 Instrument Description: The Multiple Opening/Closing Net and Environmental Sensing System or MOCNESS is a family of net systems based on the Tucker Trawl principle. The MOCNESS-1 carries nine 1-m2 nets usually of 335 micrometer mesh and is intended for use with the macrozooplankton. All nets are black to reduce contrast with the background. A motor/toggle release assembly is mounted on the top portion of the frame and stainless steel cables with swaged fittings are used to attach the net bar to the toggle release. A stepping motor in a pressure compensated case filled with oil turns the escapement crankshaft of the toggle release which sequentially releases the nets to an open then closed position on command from the surface. -- from the MOCNESS Operations Manual (1999 + 2003). Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/NETT0097/
Cruise: KM1910
KM1910
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel
KM1910
Matthew J. Church
University of Montana
https://datadocs.bco-dmo.org/docs/305/Chief_Sci_KM1910/data_docs/matt_church_EAGER_cruise_plan_06_17_2019.pdf
Report describing KM1910
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel