1b:I[42426,["/_next/static/immutable/chunks/1xctgzqre-wsy.js","/_next/static/immutable/chunks/06_-wz6pdtp2b.js","/_next/static/immutable/chunks/30e52g49hg40p.js","/_next/static/immutable/chunks/02yq6tul2z-oh.js","/_next/static/immutable/chunks/10bx_ec2vj7n_.js","/_next/static/immutable/chunks/33bfn03fo8xnt.js","/_next/static/immutable/chunks/0tfjf78scu61b.js","/_next/static/immutable/chunks/31xnvej4n1bi9.js"],"default"] 1c:Teb1,NSF Award Abstract: Planktonic ciliates are key members of marine food webs where they serve diverse roles, including as food chain links between smaller microbes and larger plankton. Due to their small size and difficulties in identifying and cultivating them, we know less about ciliate diversity and distributions in the ocean than we do about larger organisms such as fish and invertebrates. Previous work from this team measured ciliate diversity in coastal waters and found that distinct genetic variants were separated in time and space in a way that could be related to factors such as ocean temperature, salinity, and depth gradients. Many questions remained unanswered, and it is important to understand the environmental factors that control the diversity and distribution of plankton such as ciliates to predict how these organisms may respond to a changing enviroment in the coming decades. This project focuses on: 1) how ciliate species are delineated using single-cell genomics and transcriptomics; 2) DNA-based studies of all ciliates and other planktonic members of the SAR clade (Stramenopila, Alveolata, Rhizaria), which will provide ecological context; 3) in situ gene expression by single-cell and meta- transcriptomics; and 4) laboratory studies of gene expression in cultivated ciliate species. This project involves training of postdoctoral scholars, graduate students, and undergraduates. The researchers are committed to creating diverse and inclusive research labs; recruitment of participants will be done through partnership with appropriate groups on our campuses. The project integrates with summer Research Experiences for Undergraduates (REU) activities at both Smith College and UCONN (including the UCONN/Mystic Aquarium joint REU), which are especially focused on underrepresented students. This project also enhances efforts to broaden understanding of biodiversity in partnership with the UCONN Noyce Scholars Program, which facilitates career-changing STEM professionals to become teachers in underserved secondary schools. This project will assess distributions of reproductively-isolated species, determined using a new method to characterize regions of the ciliate germline genome. Furthermore, it will use phylogenomic methods to identify clade-specific transcripts (e.g. those of spirotrich ciliates) within metatranscriptomes from the shelf environment and to expand knowledge of ciliate function with single-cell transcriptomics of field-collected cells. These approaches will be a substantial improvement over the culture-based methods that are potentially biased towards "weedy" species in the ocean. The combination of definitive species identification with assessment of function via single-cell and meta- transcriptomics promises to provide significant advances in marine plankton ecology. The research focuses on two broad questions: 1) does the observed high diversity in phylogenetically-informative genes reflect reproductive isolation and functional differentiation in planktonic ciliates? and 2) do different co-occurring species of planktonic ciliates show substantial functional differences that correspond to different niches in the ocean? The project assesses species boundaries (i.e. reproductive isolation) through analyses of patterns in the germline micronuclei of planktonic ciliate morphospecies; characterizes transitions of closely-related ciliates across ecological gradients in the ocean; and examines functional differences within and between species, and in communities, through analyses of transcriptomics. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.6:["$","$L1b",null,{"dataset":{"_id":"854554","description":"","problem":"","acquisition":"

Samples were collected at the UConn dock in Groton, CT, USA (41˚ 18' 59\" N, 72˚ 03'39\" W). Dock sampling was conducted weekly using a 20 um mesh plankton net, bulb thermometer, and temperature-corrected salinity refractometer. On some dates, cells were picked for single-cell genome/transcriptomes. There is a gap in the data between 2020-03-20 and 2020-05-01 when sampling was suspended due to COVID-19.

\n","processing":"

BCO-DMO Processing:
\n- removed empty rows;
\n- renamed fields to conform with BCO-DMO naming conventions;
\n- removed the Notes column (empty);
\n- changed date format to YYYY-MM-DD;
\n- added ISO8601 datetime (UTC) fields.

\n","bcodmoProcessing":"","title":"Temperature and salinity measured at the UConn dock in Groton, CT, USA, associated with DNA collection","nickname":"Weekly dock samples","dataUrl":"","currentState":"Final no updates expected","currentStateId":"7","validated":true,"restricted":false,"version":"1","version_date":"2021-06-23T04:00:00.000Z","abstract":"This dataset includes temperature and salinity measurements collected from the UConn dock in Groton CT, USA (41˚ 18' 59\" N, 72˚ 03'39\" W), in association with DNA collection. Measurements were made weekly from January 2020 to April 2021.","citation":"McManus, G., Katz, L. A., Santoferrara, L. (2021) Temperature and salinity measured at the UConn dock in Groton, CT, USA, associated with DNA collection. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-06-23 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.854554.1 [access date]","citationParts":{"identifier":"doi:10.26008/1912/bco-dmo.854554.1","versionDate":"2021-06-23","versionId":"1","year":"2021","title":"Temperature and salinity measured at the UConn dock in Groton, CT, USA, associated with DNA collection","authors":"McManus, G., Katz, L. A., Santoferrara, L.","publisher":"Biological and Chemical Oceanography Data Management Office (BCO-DMO)."},"doi":"10.26008/1912/bco-dmo.854554.1","release_date":null,"field_dataset_location":"","temporal_extent":{"start":"2020-01-24T05:00:00.000Z","end":"2021-04-06T04:00:00.000Z"},"temporal":{"resolution":"time:unitDay","type":"time:Interval","start":{"value":"1579842000","label":"2020-01-24"},"end":{"value":"1617681600","label":"2021-04-06"}},"spatial_extent":{"shape":"point","center":{"latitude":41.31639,"longitude":-72.06083},"wkt":" POINT (-72.06083 41.31639)","north":0,"east":0,"south":0,"west":0},"dataset_type":["Other Field Results"],"keywords":["oceans"],"data_files":[{"id":"903392","title":"weekly_dock_samples.csv","url":"https://datadocs.bco-dmo.org/dataset/854554/file/m77kmN5cgp51O/weekly_dock_samples.csv","showTableUrl":"https://www.bco-dmo.org/dataset/854554/data/view/903392","description":"Primary data file for dataset ID 854554","filename":"weekly_dock_samples.csv","bytesize":3901,"checksum":{"value":"7fd3590572b07ea9b292dd603835e442","Algorithm":{"_id":"190","name":"MD5"}},"mimetype":"text/csv","fileType":{"_id":"192","name":"Comma Separated Values (.csv)"},"isErddap":"","date":"","isPublicDuringEmbargo":false,"primaryDataFile":true,"showTable":true,"restricted":false}],"supplemental_files":[],"roles":[{"role":{"_id":"138","name":"Principal Investigator"},"performedBy":{"_id":"51375","name":"George McManus"},"performedAtOrganization":{"_id":"85","name":"University of Connecticut"}},{"role":{"_id":"140","name":"Co-Principal Investigator"},"performedBy":{"_id":"51376","name":"Laura A. Katz"},"performedAtOrganization":{"_id":"255","name":"Smith College"}},{"role":{"_id":"140","name":"Co-Principal Investigator"},"performedBy":{"_id":"626644","name":"Luciana Santoferrara"},"performedAtOrganization":{"_id":"85","name":"University of Connecticut"}},{"role":{"_id":"145","name":"BCO-DMO Data Manager"},"performedBy":{"_id":"51498","name":"Shannon Rauch"},"performedAtOrganization":{"_id":"191","name":"Woods Hole Oceanographic Institution"}}],"deployments":[],"funding":[{"_id":"826125","name":"OCE-1924570","fundingSource":{"_id":"355","name":"NSF Division of Ocean Sciences"},"url":"https://www.nsf.gov/awardsearch/show-award?AWD_ID=1924570"},{"_id":"826130","name":"OCE-1924527","fundingSource":{"_id":"355","name":"NSF Division of Ocean Sciences"},"url":"https://www.nsf.gov/awardsearch/show-award?AWD_ID=1924527"}],"instruments":[{"_id":"854563","name":"20 um mesh plankton net","description":"","type_id":"525","type_name":"Plankton Net","type_desc":"A Plankton Net is a generic term for a sampling net that is used to collect plankton. It is used only when detailed instrument documentation is not available.","type_acronym":"Plankton Net"},{"_id":"854565","name":"temperature-corrected salinity refractometer","description":"","type_id":"679","type_name":"Refractometer","type_desc":"A refractometer is a laboratory or field device for the measurement of an index of refraction (refractometry). The index of refraction is calculated from Snell's law and can be calculated from the composition of the material using the Gladstone-Dale relation.\r\n\r\nIn optics the refractive index (or index of refraction) n of a substance (optical medium) is a dimensionless number that describes how light, or any other radiation, propagates through that medium.","type_acronym":"Refractometer"},{"_id":"854564","name":"bulb thermometer","description":"","type_id":"725867","type_name":"Thermometer","type_desc":"A device designed to measure temperature.","type_acronym":""}],"parameters":[{"_id":"854597","name":"Date","format":"%Y-%m-%d","frictionlessType":"date","description":"

Date of sampling (local time zone EST/EDT); format: YYYY-MM-DD

","units":{"_id":"","name":"unitless"},"type":{"_id":"1070","name":"date","description":"

date; generally reported in GMT as YYYYMMDD (year; month; day); also as MMDD (month; day); EqPac dates are local Hawaii time. ISO_Date format is YYYY-MM-DD (http://www.iso.org/iso/home/standards/iso8601.htm)

"}},{"_id":"854598","name":"Time_of_High_Tide_local","format":"%H:%M","frictionlessType":"time","description":"

Time of high tide (local time zone EST/EDT); format: hh:mm

","units":{"_id":"","name":"unitless"},"type":{"_id":"1075","name":"time","description":"

Time GMT defined as HHMM; time of day; generally reported in GMT as HHMM (hours and minutes) but may also be HH.H (decimal hours) or HHMMSS (hours; minutes and seconds); in EqPac time is reported as Hawaii local time

"}},{"_id":"854599","name":"Time_of_Dock_Tow_local","format":"%H:%M","frictionlessType":"time","description":"

Time of dock tow (local time zone EST/EDT); format: hh:mm

","units":{"_id":"","name":"unitless"},"type":{"_id":"1075","name":"time","description":"

"}},{"_id":"854600","name":"Temperature","format":"","frictionlessType":"number","description":"

Water temperature

","units":{"_id":"","name":"degrees Celsius"},"type":{"_id":"733","name":"temperature","description":"

water temperature at measurement depth

"}},{"_id":"854601","name":"Salinity","format":"","frictionlessType":"number","description":"

Water salinity

","units":{"_id":"","name":"parts per thousand (PPT)"},"type":{"_id":"955","name":"sal","description":"

Salinity: The amount (mass or quantity of matter) of salt per amount (volume or mass) of a body of water.

"}},{"_id":"854602","name":"ISO_DateTime_UTC_High_Tide","format":"%Y-%m-%dT%H:%MZ","frictionlessType":"datetime","description":"

Date and time (UTC) of high tide in ISO8601 format: YYYY-MM-DDThh:mmZ

","units":{"_id":"","name":"unitless"},"type":{"_id":"1908","name":"ISO_DateTime_UTC","description":"

Date/Time (UTC) ISO formatted
\nThis standard is based on ISO 8601:2004(E) and takes on any of the following forms:
\n2009-08-30T09:05:00[.xx] (local time)
\n2009-08-30T14:05:00[.xx]Z (UTC time)
\n2009-08-30T14:05:00[.xx]-05:00
\nThe dashes and the colons can be dropped.
\nThe T can also be dropped \"by mutual agreement\", but one needs the trailing Z if the time is UTC.

Sample ISO_datetime_utc:
\n2009-08-30T14:05:00[.xx]Z (UTC time)

Format: YYYY-mm-ddTHH:MM:SS[.xx]Z (UTC time)

"}},{"_id":"854603","name":"ISO_DateTime_UTC_Dock_Tow","format":"%Y-%m-%dT%H:%MZ","frictionlessType":"datetime","description":"

Date and time (UTC) of dock tow in ISO8601 format: YYYY-MM-DDThh:mmZ

","units":{"_id":"","name":"unitless"},"type":{"_id":"1908","name":"ISO_DateTime_UTC","description":"

Sample ISO_datetime_utc:
\n2009-08-30T14:05:00[.xx]Z (UTC time)

Format: YYYY-mm-ddTHH:MM:SS[.xx]Z (UTC time)

"}}],"related_publications":[],"related_datasets":[],"services":[{"grouping":"","items":[{"name":"View Data","description":"An in-browser view of the primary tabular data file","url":"https://www.bco-dmo.org/dataset/854554/data/view","contentType":"text/html","actionType":"view"}]},{"grouping":"Metadata","items":[{"name":"HTML","description":"A simple HTML page of the dataset metadata","url":"https://www.bco-dmo.org/dataset/854554/description","contentType":"text/html","actionType":"view"},{"name":"PDF","description":"A PDF of the HTML dataset metadata","url":"https://www.bco-dmo.org/dataset/854554/Dataset_description.pdf","contentType":"application/pdf","actionType":"download"},{"name":"ISO 19115-2 (NOAA Profile)","description":"","url":"https://www.bco-dmo.org/dataset/854554/iso","contentType":"application/xml","actionType":"download"}]}],"programs":[],"projects":[{"_id":"826126","name":"Collaborative Research: Combining single-cell and community 'omics' to test hypotheses about diversity and function of planktonic ciliates","acronym":"Ciliate Omics","description":"$1c"}],"archives":[{"id_value":"10.26008/1912/bco-dmo.854554.1","url":"https://doi.org/10.26008/1912/bco-dmo.854554.1","date_assigned":"2021-06-28","version_date":"2021-06-23","archiver":"Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)","archiver_id":"272"}],"license":{"name":"Creative Commons Attribution 4.0","id":"CC-BY-4.0","uri":"https://creativecommons.org/licenses/by/4.0/","spdxUri":"https://spdx.org/licenses/CC-BY-4.0"},"metrics":{"views":{"count":138},"downloads":{"count":394}}},"fileId":"903392"}]