http://lod.bco-dmo.org/id/dataset/854554 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2021-06-23 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Temperature and salinity measured at the UConn dock in Groton, CT, USA, associated with DNA collection 2021-06-23 publication 2021-06-23 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2021-06-28 publication https://doi.org/10.26008/1912/bco-dmo.854554.1 George McManus University of Connecticut principalInvestigator Laura A. Katz Smith College principalInvestigator Luciana Santoferrara University of Connecticut principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: McManus, G., Katz, L. A., Santoferrara, L. (2021) Temperature and salinity measured at the UConn dock in Groton, CT, USA, associated with DNA collection. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-06-23 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.854554.1 [access date] Weekly dock samples Dataset Description: Methods and Sampling: &lt;p&gt;Samples were collected at the UConn dock in Groton, CT, USA (41˚ 18' 59&quot; N, 72˚ 03'39&quot; W). Dock sampling was conducted weekly using a 20 um mesh plankton net, bulb thermometer, and temperature-corrected salinity refractometer. On some dates, cells were picked for single-cell genome/transcriptomes. There is a gap in the data between 2020-03-20 and 2020-05-01 when sampling was suspended due to COVID-19.&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1924570 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1924570 Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1924527 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1924527 completed George McManus University of Connecticut 860-405-8602 Department of Marine Sciences, University of Connecticut 1080 Shennecossett Rd. Groton CT 06340 USA george.mcmanus@uconn.edu pointOfContact Laura A. Katz Smith College 413-585-3825 Department of Biological Sciences Smith College Burton Hall 201 Northampton MA 01063 USA lkatz@smith.edu pointOfContact Luciana Santoferrara University of Connecticut 516-463-7718 Biology Dept Room 301 Gittleson Hall Hempstead NY 11549-1000 USA luciana.santoferrara@hofstra.edu pointOfContact asNeeded Dataset Version: 1 Unknown Date Time_of_High_Tide_local Time_of_Dock_Tow_local Temperature Salinity ISO_DateTime_UTC_High_Tide ISO_DateTime_UTC_Dock_Tow 20 um mesh plankton net temperature-corrected salinity refractometer bulb thermometer theme None, User defined date time of day water temperature at measurement depth salinity ISO_DateTime_UTC featureType BCO-DMO Standard Parameters Plankton Net Refractometer Thermometer instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Collaborative Research: Combining single-cell and community &#039;omics&#039; to test hypotheses about diversity and function of planktonic ciliates http://microzooplankton.uconn.edu Collaborative Research: Combining single-cell and community &#039;omics&#039; to test hypotheses about diversity and function of planktonic ciliates <p><em>NSF Award Abstract:</em><br /> Planktonic ciliates are key members of marine food webs where they serve diverse roles, including as food chain links between smaller microbes and larger plankton. Due to their small size and difficulties in identifying and cultivating them, we know less about ciliate diversity and distributions in the ocean than we do about larger organisms such as fish and invertebrates. Previous work from this team measured ciliate diversity in coastal waters and found that distinct genetic variants were separated in time and space in a way that could be related to factors such as ocean temperature, salinity, and depth gradients. Many questions remained unanswered, and it is important to understand the environmental factors that control the diversity and distribution of plankton such as ciliates to predict how these organisms may respond to a changing enviroment in the coming decades. This project focuses on: 1) how ciliate species are delineated using single-cell genomics and transcriptomics; 2) DNA-based studies of all ciliates and other planktonic members of the SAR clade (Stramenopila, Alveolata, Rhizaria), which will provide ecological context; 3) in situ gene expression by single-cell and meta- transcriptomics; and 4) laboratory studies of gene expression in cultivated ciliate species. This project involves training of postdoctoral scholars, graduate students, and undergraduates. The researchers are committed to creating diverse and inclusive research labs; recruitment of participants will be done through partnership with appropriate groups on our campuses. The project integrates with summer Research Experiences for Undergraduates (REU) activities at both Smith College and UCONN (including the UCONN/Mystic Aquarium joint REU), which are especially focused on underrepresented students. This project also enhances efforts to broaden understanding of biodiversity in partnership with the UCONN Noyce Scholars Program, which facilitates career-changing STEM professionals to become teachers in underserved secondary schools.</p> <p>This project will assess distributions of reproductively-isolated species, determined using a new method to characterize regions of the ciliate germline genome. Furthermore, it will use phylogenomic methods to identify clade-specific transcripts (e.g. those of spirotrich ciliates) within metatranscriptomes from the shelf environment and to expand knowledge of ciliate function with single-cell transcriptomics of field-collected cells. These approaches will be a substantial improvement over the culture-based methods that are potentially biased towards "weedy" species in the ocean. The combination of definitive species identification with assessment of function via single-cell and meta- transcriptomics promises to provide significant advances in marine plankton ecology. The research focuses on two broad questions: 1) does the observed high diversity in phylogenetically-informative genes reflect reproductive isolation and functional differentiation in planktonic ciliates? and 2) do different co-occurring species of planktonic ciliates show substantial functional differences that correspond to different niches in the ocean? The project assesses species boundaries (i.e. reproductive isolation) through analyses of patterns in the germline micronuclei of planktonic ciliate morphospecies; characterizes transitions of closely-related ciliates across ecological gradients in the ocean; and examines functional differences within and between species, and in communities, through analyses of transcriptomics.</p> <p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p> Ciliate Omics largerWorkCitation project eng; USA oceans -72.06083 -72.06083 41.31639 41.31639 2020-01-24 2021-04-06 New England continental shelf 0 BCO-DMO catalogue of parameters from Temperature and salinity measured at the UConn dock in Groton, CT, USA, associated with DNA collection Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/854597.rdf Name: Date Units: unitless Description: <p>Date of sampling (local time zone EST/EDT); format: YYYY-MM-DD</p> http://lod.bco-dmo.org/id/dataset-parameter/854598.rdf Name: Time_of_High_Tide_local Units: unitless Description: <p>Time of high tide (local time zone EST/EDT); format: hh:mm</p> http://lod.bco-dmo.org/id/dataset-parameter/854599.rdf Name: Time_of_Dock_Tow_local Units: unitless Description: <p>Time of dock tow (local time zone EST/EDT); format: hh:mm</p> http://lod.bco-dmo.org/id/dataset-parameter/854600.rdf Name: Temperature Units: degrees Celsius Description: <p>Water temperature</p> http://lod.bco-dmo.org/id/dataset-parameter/854601.rdf Name: Salinity Units: parts per thousand (PPT) Description: <p>Water salinity</p> http://lod.bco-dmo.org/id/dataset-parameter/854602.rdf Name: ISO_DateTime_UTC_High_Tide Units: unitless Description: <p>Date and time (UTC) of high tide in ISO8601 format: YYYY-MM-DDThh:mmZ</p> http://lod.bco-dmo.org/id/dataset-parameter/854603.rdf Name: ISO_DateTime_UTC_Dock_Tow Units: unitless Description: <p>Date and time (UTC) of dock tow in ISO8601 format: YYYY-MM-DDThh:mmZ</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 3901 https://darchive.mblwhoilibrary.org/bitstream/1912/27277/1/dataset-854554_weekly-dock-samples__v1.tsv download https://doi.org/10.26008/1912/bco-dmo.854554.1 download onLine dataset &lt;p&gt;Samples were collected at the UConn dock in Groton, CT, USA (41˚ 18' 59&quot; N, 72˚ 03'39&quot; W). Dock sampling was conducted weekly using a 20 um mesh plankton net, bulb thermometer, and temperature-corrected salinity refractometer. On some dates, cells were picked for single-cell genome/transcriptomes. There is a gap in the data between 2020-03-20 and 2020-05-01 when sampling was suspended due to COVID-19.&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;BCO-DMO Processing:&lt;br /&gt; - removed empty rows;&lt;br /&gt; - renamed fields to conform with BCO-DMO naming conventions;&lt;br /&gt; - removed the Notes column (empty);&lt;br /&gt; - changed date format to YYYY-MM-DD;&lt;br /&gt; - added ISO8601 datetime (UTC) fields.&lt;/p&gt; Specified by the Principal Investigator(s) asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 20 um mesh plankton net 20 um mesh plankton net PI Supplied Instrument Name: 20 um mesh plankton net Instrument Name: Plankton Net Instrument Short Name:Plankton Net Instrument Description: A Plankton Net is a generic term for a sampling net that is used to collect plankton. It is used only when detailed instrument documentation is not available. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/22/ temperature-corrected salinity refractometer temperature-corrected salinity refractometer PI Supplied Instrument Name: temperature-corrected salinity refractometer Instrument Name: Refractometer Instrument Short Name:Refractometer Instrument Description: A refractometer is a laboratory or field device for the measurement of an index of refraction (refractometry). The index of refraction is calculated from Snell's law and can be calculated from the composition of the material using the Gladstone-Dale relation. In optics the refractive index (or index of refraction) n of a substance (optical medium) is a dimensionless number that describes how light, or any other radiation, propagates through that medium. bulb thermometer bulb thermometer PI Supplied Instrument Name: bulb thermometer Instrument Name: Thermometer Instrument Short Name: Instrument Description: A device designed to measure temperature.