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&lt;p&gt;Given the dearth of knowledge of the lower oceanic crust, this project is poised to transform our understanding of life in this vast environment. The project assesses metabolic functions within all three domains of life in this crustal biosphere, with a focus on nutrient cycling and evaluation of connections to other deep marine microbial habitats. The lower ocean crust represents a potentially vast biosphere whose microbial constituents and the biogeochemical cycles they mediate are likely linked to deep ocean processes through faulting and subsurface fluid flow. Atlantis Bank represents a tectonic window that exposes lower oceanic crust directly at the seafloor. This enables seafloor drilling and research on an environment that can transform our understanding of connections between the deep subseafloor biosphere and the rest of the ocean. Preliminary analysis of recovered rocks from Expedition 360 suggests the interaction of seawater with the lower oceanic crust creates varied geochemical conditions capable of supporting diverse microbial life by providing nutrients and chemical energy. This project is the first interdisciplinary investigation of the microbiology of all 3 domains of life in basement samples that combines diversity and &quot;meta-omics&quot; analyses, analysis of nutrient addition experiments, high-throughput culturing and physiological analyses of isolates, including evaluation of their ability to utilize specific carbon sources, Raman spectroscopy, and lipid biomarker analyses. Comparative genomics are used to compare genes and pathways relevant to carbon cycling in these samples to data from published studies of other deep-sea environments. The collected samples present a rare and time-sensitive opportunity to gain detailed insights into microbial life, available carbon and energy sources for this life, and of dispersal of microbiota and connections in biogeochemical processes between the lower oceanic crust and the overlying aphotic water column.&lt;/p&gt;
&lt;p&gt;&lt;strong&gt;About the study area:&lt;/strong&gt;&lt;/p&gt;
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&amp;lt;p&amp;gt;Raman spectra were collected using a Horiba LabRam HR confocal Raman system using a blue laser with a wavelength of 473 nm, a 600 grooves/mm grating, and a 50x objective. The slit size was 150 µm and the confocal hole diameter was 1mm. This setting was chosen to minimize oxidation of the Fe-Mn oxide that was intergrown with the organic inclusions&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Background was subtracted using the software code LabSpec 6&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Spectral artifacts from oxidation of the Fe-Mn oxide and thin section polymer.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;BCO-DMO processing notes:&amp;lt;/p&amp;gt;

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&amp;lt;li&amp;gt;Adjusted column names to comply with database requirements&amp;lt;/li&amp;gt;
&amp;lt;li&amp;gt;Merged 3 tables into 1 (based on wavenumbers).&amp;lt;/li&amp;gt;
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