http://lod.bco-dmo.org/id/dataset/855162
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2021-07-06
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Proportion of infected polyps at Days 31, 52 and 69 in newly settled polyps of Antillogorgia bipinnata inoculated with one of five genotypes of Breviolum antillogorgium and reared at 26 and 30 degrees Celsius.
2021-11-16
publication
2021-11-16
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2021-11-16
publication
https://doi.org/10.26008/1912/bco-dmo.855162.1
Casey terHorst
California State University Northridge
principalInvestigator
Mary Alice Coffroth
State University of New York at Buffalo
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Coffroth, M. A., terHorst, C. (2021) Proportion of infected polyps at Days 31, 52 and 69 in newly settled polyps of Antillogorgia bipinnata inoculated with one of five genotypes of Breviolum antillogorgium and reared at 26 and 30 degrees Celsius. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-11-16 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.855162.1 [access date]
Infected polyps Dataset Description: Methods and Sampling: <p>Larvae were collected from <em>Antillogorgia bipinnata </em>colonies that were maintained in seawater tables at the Keys Marine Lab on Long Key, FL.&nbsp; <em>Antillogorgia bipinnata </em>colonies were initially collected from Tennessee Reef (N 24° 45.150'&nbsp; W 81° 45.275').</p>
<p><em>Breviolum antillogorgium </em>cultures, representing five genotypes, were used to infect polyps reared from larvae collected from <em>A. bipinnata</em>. Three cultures had been grown at 26-degree C since isolation in 2016 (G1 [16-0590F], G2 [16-0875] and G3 [16-1631]) and two had been grown at 30-degree C since isolation in 2016 (G4 [16-0587] and G5 [16-0763]).&nbsp; Polyps from all treatments were reared at both 26 and 30 degree C in the lab under 12:12 light dark cycles.&nbsp; Containers with polyps were inoculated on Day 1 and Day 5. Starting on Day 32, containers were inoculated three times a week with water changes.&nbsp; Polyp location in the containers was mapped and infection status for each individual polyp was recorded every 3-4 days through visual observation as indicated by a light brown coloration in the polyp. At the end of the experiment (Day 69) infection status was based on cell counts.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1559286 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1559286
completed
Casey terHorst
California State University Northridge
818-677-3352
Department of Biology 18111 Nordhoff Street
Northridge
CA
91330-8303
USA
casey.terhorst@csun.edu
pointOfContact
Mary Alice Coffroth
State University of New York at Buffalo
716-645-4871
Department of Geology 126 Cooke Hall
Buffalo
NY
14260
USA
Coffroth@buffalo.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Genotype
Temperature
Container
Day
Proportion_infected
Percent_infected
Leica dissecting microscope
theme
None, User defined
sample description
temperature of sample or a generic measurement (not in-situ water temp)
featureType
BCO-DMO Standard Parameters
Microscope - Optical
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
RUI: Collaborative Research: Genetic variation as a driver of host and symbiont response to increased temperature on coral reefs
https://osprey.bco-dmo.org/project/632538
RUI: Collaborative Research: Genetic variation as a driver of host and symbiont response to increased temperature on coral reefs
<p><em>Description from NSF award abstract:</em><br />
On coral reefs, mutualisms with single celled algae (Symbiodinium) and reef species literally and figuratively form the foundation of reef ecosystems. Coral reefs are among the most threatened ecosystems under a changing climate and are rapidly declining due to increasing levels of environmental stress, namely increased temperatures. Climate change is resulting in even warmer ocean temperatures that threaten associations between Symbiodinium and their hosts. In this project the investigators examine the genetic diversity of Symbiodinium and the potential for this important species to evolve in response to temperature. The project will also address whether the ecological and evolutionary dynamics of the Symbiodinium population affect the performance of their host. If so, this suggests that the evolution of microscopic organisms with short generation times could confer adaptation to longer-lived host species on ecologically and economically vital coral reefs. Given that diversity is already being lost on many reefs, considering how evolutionary changes in Symbiodinium will affect reef species is crucial for predicting the responses of reefs to future climate change. This project provides training for two graduate students and several undergraduates at a Hispanic-serving institution. This work includes outreach to the students and the general public through the Aquarium of Niagara, local K-12 schools, and web-based education modules.</p>
<p>The effects of evolution on contemporary ecological processes are at the forefront of research in evolutionary ecology. This project will answer the call for experiments elucidating the effects of genetic variation in Symbiodinium performance and the effect on the response of the holobiont (host and symbiont) to increased temperature. These experiments examine the effects of temperature through both ecological and evolutionary mechanisms and will determine the relative importance of adaptation and acclimatization in replicated experimental populations. The investigators will examine how genetic variation within a species (Symbiodinium antillogorgium) affects symbiont performance in culture and in the host and how this affects the response of the holobiont to increased temperature. Further, the project examines whether holobiont response to increased temperature associated with climate change depends on particular GxG host-symbiont combinations. Moreover, the investigators will examine the effects of symbiont history on mutualist hosts, which have been largely ignored in eco-evolutionary studies. These experiments provide a first step in predicting whether invertebrate hosts on coral reefs will respond to global change via adaptation of their symbionts.</p>
Host Symbiont Temp Response
largerWorkCitation
project
eng; USA
oceans
-81.754583
-81.754583
24.7525
24.7525
2021-11-16
Florida Keys, Caribbean
0
BCO-DMO catalogue of parameters from Proportion of infected polyps at Days 31, 52 and 69 in newly settled polyps of Antillogorgia bipinnata inoculated with one of five genotypes of Breviolum antillogorgium and reared at 26 and 30 degrees Celsius.
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/855198.rdf
Name: Genotype
Units: Unitless
Description: <p>Genotype of culture (based on 5 microsatellite loci) used to inoculate the polyps,</p>
http://lod.bco-dmo.org/id/dataset-parameter/855199.rdf
Name: Temperature
Units: Degrees Celsius (°C)
Description: <p>Temperature at which the polyp was reared</p>
http://lod.bco-dmo.org/id/dataset-parameter/855200.rdf
Name: Container
Units: Unitless
Description: <p>Identification of container replicate</p>
http://lod.bco-dmo.org/id/dataset-parameter/855201.rdf
Name: Day
Units: Unitless
Description: <p>Day indicates the timepoint, post-inoculation, at which proportion of infected polyps was determined.</p>
http://lod.bco-dmo.org/id/dataset-parameter/855202.rdf
Name: Proportion_infected
Units: Unitless
Description: <p>Proportion infected polyps at that timepoint and was determined based on the number of polyps alive at that time. nd indicates that there were no surviving polyps at that time point. No polyps Alive</p>
http://lod.bco-dmo.org/id/dataset-parameter/855203.rdf
Name: Percent_infected
Units: Unitless
Description: <p>Percentage infected polyps at that timepoint and was determined based on the number of polyps alive at that time. nd indicates that there were no surviving polyps at that time point. No polyps Alive</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
5594
https://darchive.mblwhoilibrary.org/bitstream/1912/27764/1/dataset-855162_proportion-infected-polyps-___-fall-2018-experiment__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.855162.1
download
onLine
dataset
<p>Larvae were collected from <em>Antillogorgia bipinnata </em>colonies that were maintained in seawater tables at the Keys Marine Lab on Long Key, FL.&nbsp; <em>Antillogorgia bipinnata </em>colonies were initially collected from Tennessee Reef (N 24° 45.150'&nbsp; W 81° 45.275').</p>
<p><em>Breviolum antillogorgium </em>cultures, representing five genotypes, were used to infect polyps reared from larvae collected from <em>A. bipinnata</em>. Three cultures had been grown at 26-degree C since isolation in 2016 (G1 [16-0590F], G2 [16-0875] and G3 [16-1631]) and two had been grown at 30-degree C since isolation in 2016 (G4 [16-0587] and G5 [16-0763]).&nbsp; Polyps from all treatments were reared at both 26 and 30 degree C in the lab under 12:12 light dark cycles.&nbsp; Containers with polyps were inoculated on Day 1 and Day 5. Starting on Day 32, containers were inoculated three times a week with water changes.&nbsp; Polyp location in the containers was mapped and infection status for each individual polyp was recorded every 3-4 days through visual observation as indicated by a light brown coloration in the polyp. At the end of the experiment (Day 69) infection status was based on cell counts.</p>
Specified by the Principal Investigator(s)
<p>Proportion and percentage of infected polyps was calculated at three timepoints and was determined based on the number of polyps alive at that time.</p>
<p>Infection status of the polyps was assessed visually using a Leica dissecting microscope approximately every 3 days from Day 1 – Day 67 and by cell counts on Day 69.&nbsp;</p>
<p>BCO-DMO processing notes:</p>
<ul>
<li>Adjusted parameter names to comply with database requirements</li>
</ul>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Leica dissecting microscope
Leica dissecting microscope
PI Supplied Instrument Name: Leica dissecting microscope PI Supplied Instrument Description:Infection status of the polyps was assessed visually using a Leica dissecting microscope approximately every 3 days from Day 1 – Day 67 and by cell counts on Day 69. See BCO-DMO dataset “Cell counts in polyps – Fall 2018 experiment” Instrument Name: Microscope - Optical Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope". Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/