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            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset/855277.rdf" xlink:actuate="onRequest">Fluorescence and Physical Indicators for Sediment Cores from a Protected island in south Wilkinson Bay in the northeast portion of Barataria Bay, Louisiana in September 2018</gmx:Anchor>
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            <gco:CharacterString>Cite this dataset as: Hayes, M. P., Sapkota, Y., Thomas, E., White, J. R., Cook, R. L. (2021) Fluorescence and Physical Indicators for Sediment Cores from a Protected island in south Wilkinson Bay in the northeast portion of Barataria Bay, Louisiana in September 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-09-08 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.855277.1 [access date]</gco:CharacterString>
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        <gco:CharacterString>Methods and Sampling: &amp;lt;p&amp;gt;Triplicate core samples were collected at three different distances from the island’s edge at sites on the North, East, South, and West sides of the island. These core samples were then broken down into 10 centimeter (cm) sections and these sections were then further divided into two subsets. The samples in the first subset were used for the soil physiochemical analysis. The samples in the second set were centrifuged to yield pore water samples that were spectroscopically analyzed.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Sample Collection: Thirty-six cores, which were 0.5 – 1 meters long, were collected on four sides (cardinal directions) of the island with a manual push-coring method using an acrylic tube (1.7 m long x 7.6 cm diameter). On each side, cores were extracted in triplicates at 16 m outland, the edge of the marsh (0 m), and 2 m inland. Upon extraction in the field, each core was sectioned in 10 cm sections, sealed in polyethylene bags, and stored on ice during transportation back to the lab, where they were stored at 4 degrees&amp;lt;sup&amp;gt; &amp;lt;/sup&amp;gt;C in the dark until analysis. &amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Surface water samples were collected 10 cm below the surface at the edge of the marsh and 16 m outland following the procedures in Haywood et al. (2018).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Soil Physiochemical Analysis: The 10 cm sections were homogenized and weighed prior to analysis to determine gravimetric moisture content, bulk density, percent organic matter, and total carbon.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Gravimetric Moisture Content: The entire 10 cm section (~30 grams) was oven-dried at 60 degrees&amp;lt;sup&amp;gt; &amp;lt;/sup&amp;gt;C until constant weight was achieved.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Bulk Density: The total weight of the dried 10 cm section of core was divided by the volume of the 10 cm section (385 cm&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt;)&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Percent Organic Matter: 1 gram of the dried 10 cm section of core was ground using a mortar and pestle. It was combusted in a Thermolyne furnace (Thermofischer Scientific, Waltham, MA) at 550 degrees&amp;lt;sup&amp;gt; &amp;lt;/sup&amp;gt;C for 4 hours. After cooling, the sample was weighed. The difference in weight following combustion determined percent organic matter.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Total Carbon: 8 milligrams (mg) of the dried 10 cm section of core was used to determine total carbon. These samples were placed into a Shimadzu Total Organic Carbon Analyzer with SSM-5000 A (Shimadzu, Columbia, MD) and combusted at 900 degrees&amp;lt;sup&amp;gt; &amp;lt;/sup&amp;gt;C for 13 minutes.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Porewater Analysis: To remove pore water from the wet soil samples, samples were centrifuged at 2578 &amp;lt;em&amp;gt;g&amp;lt;/em&amp;gt; for 10 minutes. The pore water was decanted and filtered through a 0.45-micrometer x 22-millimeter Nylon syringe filter. After filtration, the pore water was stored at 4 degrees&amp;lt;sup&amp;gt; &amp;lt;/sup&amp;gt;C in the dark until analysis. To determine the dissolved organic carbon, a Total Organic Carbon Analyzer (TOC-L) (Shimazdu, Columbia, MD) was used. A Cary 100 Spectrophotometer (Varian Inc., Palo Alto, CA) was used to collect UV–Vis absorbance spectra, and a Spex Fluorolog-3 spectrofluorometer (HORIBA Scientific, Edison, NJ) was used to collect fluorescence, methods based on Haywood et&amp;amp;nbsp;al. (2018).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;The UV–Vis absorbance spectra were collected from 200 to 600 nm using a 0.5 nm bandpass and a 1 cm quartz cell, on a Cary 100 spectrophotometer.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Fluorescence EEMs were collected using a 1 cm quartz cell with excitation wavelengths of 250 to 550 nm and emission wavelengths of 250 to 600 nm with 5 nm increments for both on a Spex Fluorolog-3 spectrofluorometer. Along with sample EEMs, blank EEMs of Milli-Qnwater were collected daily.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;To minimize temperature effects, samples were allowed to reach room temperature and shielded from light prior to analysis.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;amp;nbsp;&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/670526.rdf" xlink:title="OCE-1636052" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1636052 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1636052</gmx:Anchor>
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                <gco:CharacterString>&amp;lt;p&amp;gt;Triplicate core samples were collected at three different distances from the island’s edge at sites on the North, East, South, and West sides of the island. These core samples were then broken down into 10 centimeter (cm) sections and these sections were then further divided into two subsets. The samples in the first subset were used for the soil physiochemical analysis. The samples in the second set were centrifuged to yield pore water samples that were spectroscopically analyzed.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Sample Collection: Thirty-six cores, which were 0.5 – 1 meters long, were collected on four sides (cardinal directions) of the island with a manual push-coring method using an acrylic tube (1.7 m long x 7.6 cm diameter). On each side, cores were extracted in triplicates at 16 m outland, the edge of the marsh (0 m), and 2 m inland. Upon extraction in the field, each core was sectioned in 10 cm sections, sealed in polyethylene bags, and stored on ice during transportation back to the lab, where they were stored at 4 degrees&amp;lt;sup&amp;gt; &amp;lt;/sup&amp;gt;C in the dark until analysis. &amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Surface water samples were collected 10 cm below the surface at the edge of the marsh and 16 m outland following the procedures in Haywood et al. (2018).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Soil Physiochemical Analysis: The 10 cm sections were homogenized and weighed prior to analysis to determine gravimetric moisture content, bulk density, percent organic matter, and total carbon.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Gravimetric Moisture Content: The entire 10 cm section (~30 grams) was oven-dried at 60 degrees&amp;lt;sup&amp;gt; &amp;lt;/sup&amp;gt;C until constant weight was achieved.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Bulk Density: The total weight of the dried 10 cm section of core was divided by the volume of the 10 cm section (385 cm&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt;)&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Percent Organic Matter: 1 gram of the dried 10 cm section of core was ground using a mortar and pestle. It was combusted in a Thermolyne furnace (Thermofischer Scientific, Waltham, MA) at 550 degrees&amp;lt;sup&amp;gt; &amp;lt;/sup&amp;gt;C for 4 hours. After cooling, the sample was weighed. The difference in weight following combustion determined percent organic matter.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Total Carbon: 8 milligrams (mg) of the dried 10 cm section of core was used to determine total carbon. These samples were placed into a Shimadzu Total Organic Carbon Analyzer with SSM-5000 A (Shimadzu, Columbia, MD) and combusted at 900 degrees&amp;lt;sup&amp;gt; &amp;lt;/sup&amp;gt;C for 13 minutes.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Porewater Analysis: To remove pore water from the wet soil samples, samples were centrifuged at 2578 &amp;lt;em&amp;gt;g&amp;lt;/em&amp;gt; for 10 minutes. The pore water was decanted and filtered through a 0.45-micrometer x 22-millimeter Nylon syringe filter. After filtration, the pore water was stored at 4 degrees&amp;lt;sup&amp;gt; &amp;lt;/sup&amp;gt;C in the dark until analysis. To determine the dissolved organic carbon, a Total Organic Carbon Analyzer (TOC-L) (Shimazdu, Columbia, MD) was used. A Cary 100 Spectrophotometer (Varian Inc., Palo Alto, CA) was used to collect UV–Vis absorbance spectra, and a Spex Fluorolog-3 spectrofluorometer (HORIBA Scientific, Edison, NJ) was used to collect fluorescence, methods based on Haywood et&amp;amp;nbsp;al. (2018).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;The UV–Vis absorbance spectra were collected from 200 to 600 nm using a 0.5 nm bandpass and a 1 cm quartz cell, on a Cary 100 spectrophotometer.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Fluorescence EEMs were collected using a 1 cm quartz cell with excitation wavelengths of 250 to 550 nm and emission wavelengths of 250 to 600 nm with 5 nm increments for both on a Spex Fluorolog-3 spectrofluorometer. Along with sample EEMs, blank EEMs of Milli-Qnwater were collected daily.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;To minimize temperature effects, samples were allowed to reach room temperature and shielded from light prior to analysis.&amp;lt;/p&amp;gt;

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                <gco:CharacterString>&amp;lt;p&amp;gt;Analysis of Variance (ANOVA) was performed on R (Version 3.5.3; R Foundation for Statistical Computing, Vienna, Austria). The Tukey HSD method with Bonferonni correction was applied to the significant level (alpha = 0.003). The plots were created using ggplot2 (Wickhm, 2016) in R, too.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;PCA analysis was performed in JMP (SAS Institute, Cary, NC).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;For parallel factor analysis (PARAFAC) the pre-processed sample data were arranged into a three-way array with 114 samples, 61 excitation wavelengths in 5 nanometer (nm) increments ranging from 250 to 550 nm, and 71 emission wavelengths in 5 nm increments ranging from 250 to 600 nm. The fluorescence intensity was converted from arbitrary unites (A.U.) to Raman unites (R.U.) be-fore PARAFAC analysis. The PARAFAC analysis was performed with MATLAB R2015a (Math Works, Inc., Cambridge, MA) software program, utilizing open source drEEM toolbox version 2.0. The appropriate number of components for the dataset was determined using outlier identification and method validation techniques (i.e., least square fit and split-half analysis) following methods outlined in, with the best fit model having an explained variance of N95%. The identities of the components determined in the model were verified through comparison to previously identified component models using OpenFluor (DOM fluorescence spectral database).&amp;lt;/p&amp;gt;

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              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/827601.rdf" xlink:title="muffle furnace" xlink:actuate="onRequest">Thermolyne furnace (Thermofischer Scientific, Waltham, MA)</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>Thermolyne furnace (Thermofischer Scientific, Waltham, MA)</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Thermolyne furnace (Thermofischer Scientific, Waltham, MA) Instrument Name: muffle furnace Instrument Short Name:   Instrument Description: A muffle furnace or muffle oven (sometimes retort furnace in historical usage) is a furnace in which the subject material is isolated from the fuel and all of the products of combustion, including gases and flying ash.  A type of jacketed enclosure that is used to heat a material to significantly high temperatures while keeping it contained and fully isolated from external contaminants, chemicals or substances. Muffle furnaces are usually lined with stainless steel, making them largely corrosion-resistant.</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/628287.rdf" xlink:title="Push Corer" xlink:actuate="onRequest"></gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString></gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name:  PI Supplied Instrument Description:Acrylic tube (1.7 m long x 7.6 cm diameter) Instrument Name: Push Corer Instrument Short Name:   Instrument Description: Capable of being performed in numerous environments, push coring is just as it sounds. Push coring is simply pushing the core barrel (often an aluminum or polycarbonate tube) into the sediment by hand. A push core is useful in that it causes very little disturbance to the more delicate upper layers of a sub-aqueous sediment.

Description obtained from: http://web.whoi.edu/coastal-group/about/how-we-work/field-methods/coring/</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/527277.rdf" xlink:title="Shimadzu TOC-L Analyzer" xlink:actuate="onRequest">Shimadzu Total Organic Carbon Analyzer (TOC-L) (Shimazdu, Columbia, MD)</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>Shimadzu Total Organic Carbon Analyzer (TOC-L) (Shimazdu, Columbia, MD)</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Shimadzu Total Organic Carbon Analyzer (TOC-L) (Shimazdu, Columbia, MD) Instrument Name: Shimadzu TOC-L Analyzer Instrument Short Name:Shimadzu TOC-L   Instrument Description: A Shimadzu TOC-L Analyzer measures DOC by high temperature combustion method.

Developed by Shimadzu, the 680 degree C combustion catalytic oxidation method is now used worldwide. One of its most important features is the capacity to efficiently oxidize hard-to-decompose organic compounds, including insoluble and macromolecular organic compounds. The 680 degree C combustion catalytic oxidation method has been adopted for the TOC-L series.

http://www.shimadzu.com/an/toc/lab/toc-l2.html Community Standard Description: http://onto.nerc.ac.uk/CAST/124.html</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/707.rdf" xlink:title="Spectrophotometer" xlink:actuate="onRequest">Cary 100 Spectrophotometer (Varian Inc., Palo Alto, CA)</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>Cary 100 Spectrophotometer (Varian Inc., Palo Alto, CA)</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Cary 100 Spectrophotometer (Varian Inc., Palo Alto, CA) Instrument Name: Spectrophotometer Instrument Short Name:Spectrophotometer   Instrument Description: An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/652.rdf" xlink:title="Total Organic Carbon Analyzer" xlink:actuate="onRequest">Shimadzu Total Organic Carbon Analyzer with SSM-5000 A (Shimadzu, Columbia, MD)</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>Shimadzu Total Organic Carbon Analyzer with SSM-5000 A (Shimadzu, Columbia, MD)</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Shimadzu Total Organic Carbon Analyzer with SSM-5000 A (Shimadzu, Columbia, MD) Instrument Name: Total Organic Carbon Analyzer Instrument Short Name:TOC analyzer   Instrument Description: A unit that accurately determines the carbon concentrations of organic compounds typically by detecting and measuring its combustion product (CO2). See description document at: http://bcodata.whoi.edu/LaurentianGreatLakes_Chemistry/bs116.pdf Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      </gmi:MI_AcquisitionInformation>
  </gmi:acquisitionInformation>
</gmi:MI_Metadata>
