http://lod.bco-dmo.org/id/dataset/855573 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2021-07-13 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Effect of microplastic ingestion on heterotrophic dinoflagellate ingestion rates 2021-07-13 publication 2021-07-13 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2021-08-02 publication https://doi.org/10.26008/1912/bco-dmo.855573.1 Victoria Fulfer University of Rhode Island principalInvestigator Susanne Menden-Deuer University of Rhode Island principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Fulfer, V., Menden-Deuer, S. (2021) Effect of microplastic ingestion on heterotrophic dinoflagellate ingestion rates. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-07-13 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.855573.1 [access date] Effect of microplastic ingestion on heterotrophic dinoflagellate ingestion rates Dataset Description: Methods and Sampling: &lt;p&gt;&lt;strong&gt;Methodology:&lt;/strong&gt;&lt;br /&gt; Heterotrophic dinoflagellate species &lt;em&gt;O. marina &lt;/em&gt;and &lt;em&gt;Gyrodinium &lt;/em&gt;sp. were incubated for 5 days under two conditions: a control, fed only algal prey &lt;em&gt;I. galbana, &lt;/em&gt;and a treatment fed algal prey and microplastic particles. Samples were taken every 24 hours, with abundances of dinoflagellates, algal prey, and microplastics measured with a Beckman Coulter Counter and verified via microscopy. Ingestion rates were measured and compared between treatments.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Microplastic Ingestion Experiments:&lt;/strong&gt;&lt;br /&gt; The possibility and subsequent effects of microplastic ingestion by heterotrophic dinoflagellate species were determined using two treatment conditions: first, a treatment with microplastics, in which heterotrophic dinoflagellates were fed a mixture of algal prey and microplastic particles; and second, an algae-only control, in which heterotrophic dinoflagellates were fed algal prey.&lt;/p&gt; &lt;p&gt;For the grazing experiments, each of the three target heterotrophic dinoflagellate species was incubated with &lt;em&gt;I. galbana&lt;/em&gt; and microplastic particles, when applicable, diluted in filtered seawater (FSW) to the target concentrations. Control treatments of the two prey types in the absence of predators were incubated alongside the grazing experiments. All treatments were prepared in triplicate and in a total volume of 125 mL and incubated in 250 mL polycarbonate bottles on a 12 h: 12 h light-dark cycle at 15°C and a light intensity of 8 – 15 µmol photons m⁻² s⁻¹ on a shaker table at 60 rotations-per-minute (rpm) to reduce settling of microplastic particles.&lt;/p&gt; &lt;p&gt;Fluorescent yellow polystyrene (PS) microplastic particles ranging in diameter from 2.5 to 4.5 µm were used in all microplastic feeding experiments (Spherotech, FP-3052-2). This size range was chosen to mimic the size of the algal prey species. Microplastic particles were rinsed three times in DI water and resuspended in autoclaved, filtered seawater directly before use. For each experiment, prey (IG) control treatments were prepared in triplicate in 125 mL polycarbonate bottles with &lt;em&gt;I. galbana&lt;/em&gt; diluted in filtered seawater (FSW) to a final concentration of 70,000 - 100,000 prey cells mL⁻¹.&lt;/p&gt; &lt;p&gt;Microplastic ingestion experiments were run for up to 5 days and sampled daily. Samples of 3 mL were fixed with 10% glutaraldehyde to a final concentration of 0.1% glutaraldehyde. Heterotrophic dinoflagellates were counted via light microscopy. To measure ingestion rates, subsamples of 10 mL were taken at T0 and every 24 hours for 5 days. Abundances of prey, predators, and microplastic particles were measured with a Beckman Coulter Multisizer 3 (Beckman Coulter) using a 100 µm aperture.&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1736635 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1736635 completed Victoria Fulfer University of Rhode Island 717-713-9618 215 South Ferry Rd. Narragansett RI 02882 USA vmf713@uri.edu pointOfContact Susanne Menden-Deuer University of Rhode Island 215 South Ferry Rd. Narragansett RI 02882 USA smenden@uri.edu pointOfContact asNeeded Dataset Version: 1 Unknown Species Treatment Time Replicate Microplastics_Abundance I_galbana_Abundance Het_Dino_Abundance Het_Dino_Geometric_Mean Ingestion_Rate_of_I_galbana Ingestion_Rate_of_Microplastics Ingestion_Rate_of_All_Particles Beckman Coulter Multisizer 3 theme None, User defined species scientific name (binomial) treatment time_point replicate abundance No BCO-DMO term Zooplankton feeding featureType BCO-DMO Standard Parameters Particle Size Analyzer instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Quantifying Temperature Dependence In Growth &amp; Grazing Rates of Planktonic Herbivores https://osprey.bco-dmo.org/project/739232 Quantifying Temperature Dependence In Growth &amp; Grazing Rates of Planktonic Herbivores <p><em>NSF Award Abstract:</em><br /> Plankton, single-celled organisms that inhabit the world's oceans are responsible for the generation of oxygen, cycling energy and matter between the atmosphere and the deep ocean and are the basis for virtually all seafood harvested. These life-giving functions critically depend on the relative rates at which plankton grow and get eaten. How temperature influences those rates is essential to understand plankton responses to environmental changes and ocean dynamics. It is well established that plankton grow faster when temperatures are higher however, whether feeding has a similar temperature dependence is unknown. That means oceanographers are missing key data required to build global predictive models. This project will fill essential knowledge gaps and measure physiological rates of singled celled zooplankton across temperature gradients representing the global ocean, from polar to tropical regions and throughout the seasonal cycle. Researchers will combine laboratory experiments with specimens taken from the coastal ocean (Narragansett Bay), which is exemplary in its strong seasonal temperature variations. These data will provide a clear picture of the production capacity and activity of plankton in a global and dynamic ocean. The project supports an early career scientist, as well as graduate and undergraduate students. Scientists will continue communicating their research to the public through large-scale outreach events, education at the high-school level, and engagement through online and other media. Moreover, researchers will continue collaborating with the Metcalf Institute for Marine &amp; Environmental Reporting to support their Annual Science Immersion Workshop for Journalists and their ongoing work to disseminate research findings through web-based seminars.</p> <p>Grazing is the single largest loss factor of marine primary production and thus affects a key transfer rate between global organic and inorganic matter pools. Remarkably, data for herbivorous protist growth and grazing rates at temperatures representative of the vast polar regions and during winter and spring periods are extremely sparse. By combining laboratory experiments with ground truthing fieldwork, this project alleviates a central knowledge gap in oceanography and delivers the empirical measurements necessary to derive algorithms to incorporate temperature dependence of heterotrophic protist growth and grazing rates into biogeochemical models. The extraordinary seasonal temperature fluctuations in a temperate coastal estuary (Narragansett Bay) are exploited to measure rates of heterotrophic protists isolated from different temperatures and seasons and to quantify the temperature and acclimation responses of these ecotypes. This project delivers data urgently needed to solve the conundrum of whether herbivorous growth and predation is depressed at low temperatures, implying low trophic transfer rates and high carbon export, or if predation proceeds at rates comparable to temperate systems with primary production largely lost to predation. Large temperature gradients in the global ocean mean that cross-biome and biogeochemical models are particularly sensitive to assumptions about the temperature dependence in modeled rate processes. Establishment of the dependence of heterotrophic plankton physiological rates (growth and grazing) to gradients of temperature, mimicking realistic conditions experienced by plankton in a changing ocean, is a key step towards integrating much needed biological information in biogeochemical modeling efforts. This project makes a significant contribution to linking ecological research with ecosystem models by providing empirically rooted algorithms of the temperature dependence of protistan herbivory and growth rates, key processes in the transformation of organic matter in global biogeochemical cycles and tools critically missing in ecosystem models.</p> Planktonic Herbivore Temp Dependence largerWorkCitation project eng; USA oceans -71.4495 -71.4495 41.4501 41.4501 2020-11-09 2020-11-15 Narragansett Bay 0 BCO-DMO catalogue of parameters from Effect of microplastic ingestion on heterotrophic dinoflagellate ingestion rates Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/855607.rdf Name: Species Units: unitless Description: <p>Dinoflagellate species</p> http://lod.bco-dmo.org/id/dataset-parameter/855608.rdf Name: Treatment Units: unitless Description: <p>Experimental treatment: IG = fed algae only; MP + IG = fed algae and Microplastics</p> http://lod.bco-dmo.org/id/dataset-parameter/855609.rdf Name: Time Units: days Description: <p>Time point sample taken</p> http://lod.bco-dmo.org/id/dataset-parameter/855610.rdf Name: Replicate Units: unitless Description: <p>Experimental replicate id (A, B, C)</p> http://lod.bco-dmo.org/id/dataset-parameter/855611.rdf Name: Microplastics_Abundance Units: per milliliter (mL-1) Description: <p>Abundance of Microplastics particles</p> http://lod.bco-dmo.org/id/dataset-parameter/855612.rdf Name: I_galbana_Abundance Units: per milliliter (mL-1) Description: <p>Abundance of algal prey I. galbana</p> http://lod.bco-dmo.org/id/dataset-parameter/855613.rdf Name: Het_Dino_Abundance Units: per milliliter (mL-1) Description: <p>Abundance of heterotrophic dinoflagellate</p> http://lod.bco-dmo.org/id/dataset-parameter/855614.rdf Name: Het_Dino_Geometric_Mean Units: per milliliter (mL-1) Description: <p>Calculated geometric mean for each day</p> http://lod.bco-dmo.org/id/dataset-parameter/855615.rdf Name: Ingestion_Rate_of_I_galbana Units: cells per predator per day Description: <p>Daily rate het. Dino ingested algal prey</p> http://lod.bco-dmo.org/id/dataset-parameter/855616.rdf Name: Ingestion_Rate_of_Microplastics Units: particles per predator per day Description: <p>Daily rate het. Dino ingested microplastics</p> http://lod.bco-dmo.org/id/dataset-parameter/855617.rdf Name: Ingestion_Rate_of_All_Particles Units: particles per predator per day Description: <p>Daily rate het. Dino ingested algal prey and microplastics</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 4960 https://darchive.mblwhoilibrary.org/bitstream/1912/27395/1/dataset-855573_dinoflagellate-microplastic-ingestion-rates__v1.tsv download https://doi.org/10.26008/1912/bco-dmo.855573.1 download onLine dataset &lt;p&gt;&lt;strong&gt;Methodology:&lt;/strong&gt;&lt;br /&gt; Heterotrophic dinoflagellate species &lt;em&gt;O. marina &lt;/em&gt;and &lt;em&gt;Gyrodinium &lt;/em&gt;sp. were incubated for 5 days under two conditions: a control, fed only algal prey &lt;em&gt;I. galbana, &lt;/em&gt;and a treatment fed algal prey and microplastic particles. Samples were taken every 24 hours, with abundances of dinoflagellates, algal prey, and microplastics measured with a Beckman Coulter Counter and verified via microscopy. Ingestion rates were measured and compared between treatments.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Microplastic Ingestion Experiments:&lt;/strong&gt;&lt;br /&gt; The possibility and subsequent effects of microplastic ingestion by heterotrophic dinoflagellate species were determined using two treatment conditions: first, a treatment with microplastics, in which heterotrophic dinoflagellates were fed a mixture of algal prey and microplastic particles; and second, an algae-only control, in which heterotrophic dinoflagellates were fed algal prey.&lt;/p&gt; &lt;p&gt;For the grazing experiments, each of the three target heterotrophic dinoflagellate species was incubated with &lt;em&gt;I. galbana&lt;/em&gt; and microplastic particles, when applicable, diluted in filtered seawater (FSW) to the target concentrations. Control treatments of the two prey types in the absence of predators were incubated alongside the grazing experiments. All treatments were prepared in triplicate and in a total volume of 125 mL and incubated in 250 mL polycarbonate bottles on a 12 h: 12 h light-dark cycle at 15°C and a light intensity of 8 – 15 µmol photons m⁻² s⁻¹ on a shaker table at 60 rotations-per-minute (rpm) to reduce settling of microplastic particles.&lt;/p&gt; &lt;p&gt;Fluorescent yellow polystyrene (PS) microplastic particles ranging in diameter from 2.5 to 4.5 µm were used in all microplastic feeding experiments (Spherotech, FP-3052-2). This size range was chosen to mimic the size of the algal prey species. Microplastic particles were rinsed three times in DI water and resuspended in autoclaved, filtered seawater directly before use. For each experiment, prey (IG) control treatments were prepared in triplicate in 125 mL polycarbonate bottles with &lt;em&gt;I. galbana&lt;/em&gt; diluted in filtered seawater (FSW) to a final concentration of 70,000 - 100,000 prey cells mL⁻¹.&lt;/p&gt; &lt;p&gt;Microplastic ingestion experiments were run for up to 5 days and sampled daily. Samples of 3 mL were fixed with 10% glutaraldehyde to a final concentration of 0.1% glutaraldehyde. Heterotrophic dinoflagellates were counted via light microscopy. To measure ingestion rates, subsamples of 10 mL were taken at T0 and every 24 hours for 5 days. Abundances of prey, predators, and microplastic particles were measured with a Beckman Coulter Multisizer 3 (Beckman Coulter) using a 100 µm aperture.&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;&lt;strong&gt;Data Processing:&lt;/strong&gt;&lt;br /&gt; Ingestion rates (I) were calculated via the ingestion rate equation in Frost 1972.&amp;nbsp; Ingestion rates of plastic were also calculated using the change in plastic concentration (&lt;em&gt;N&lt;sub&gt;i&lt;/sub&gt;&lt;/em&gt; - &lt;em&gt;N&lt;sub&gt;f&lt;/sub&gt;&lt;/em&gt;).&lt;/p&gt; &lt;p&gt;&lt;strong&gt;BCO-DMO Processing:&lt;/strong&gt;&lt;br /&gt; - replaced 'NaN' with 'nd' (no data);&lt;br /&gt; - renamed fields to conform with BCO-DMO naming conventions (removed spaces and special characters)&lt;br /&gt; &amp;nbsp;&lt;/p&gt; Specified by the Principal Investigator(s) asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact Beckman Coulter Multisizer 3 Beckman Coulter Multisizer 3 PI Supplied Instrument Name: Beckman Coulter Multisizer 3 PI Supplied Instrument Description:Abundances of prey, predators, and microplastic particles were measured with a Beckman Coulter Multisizer 3 (Beckman Coulter) using a 100 µm aperture. Instrument Name: Particle Size Analyzer Instrument Short Name: Instrument Description: Particle size analysis, particle size measurement, or simply particle sizing is the collective name of the technical procedures, or laboratory techniques which determines the size range, and/or the average, or mean size of the particles in a powder or liquid sample.