<div><p>This dataset comprises results of respirometry experiments conducted at sea. Experimental animals were collected using either a modified tucker trawl or MOCNESS. Metabolic rates and critical oxygen pressures were measured using published methods (Birk et al., 2018; 2019; Wishner et al., 2018; Seibel et al., 2021). Briefly, individual specimens were placed in darkened sealed chambers filled with 0.2 μm filtered seawater that had been treated with antibiotics (25 mg/L each of streptomycin and actinomycin) to minimize bacterial respiration. The ratio of chamber size to body mass was approximately 1:20. Oxygen content was continuously monitored using Pyroscience Firesting or Presens oxygen meters and fiber optic probes with oxygen-sensitive fluorescent spots. Oxygen meters were calibrated with air-saturated seawater and concentrated Na2SO3 solution (PO2 = 0). Chamber water was mixed with magnetic stirrers (Cole-Parmer Immersible Stirrer EW-04636-50) to ensure homogenous oxygen levels throughout the chamber. Oxygen consumption rates (MO2) were calculated from the slope of PO2 over time. Only linear regressions with an R2 > 0.8 were included. The median MO2 value from non-hypoxic oxygen levels was calculated for each individual. The Pcrit for each individual was calculated using three metrics, including the traditional "breakpoint" method, the "sub-prediction interval (sub-PI)" and the alpha-method.</p></div>
Respirometry data for pelagic crustaceans, cephalopods, and fish
Zooplankton metabolic traits
<div><p><strong>Data Processing</strong>:<br />
Data were processed in R using published tools from Birk (2020). Calculated values of critical oxygen partial pressure (Pcrit) are compared among methods that use different bin sizes and/or definitions of Pcrit, as described below.</p>
<p><strong>Pcrit_fixed_width</strong>: breakpoint Pcrit using 10 minute bins if total duration < 1000 minutes and 60 minute bins if total duration > 1000 minutes.</p>
<p><strong>Pcrit_sub_reg</strong>: sliding bin width based on PO2. Pcrit defined as intersection of conforming line and lower 95% prediction interval bound. Outliers removed.</p>
<p><strong>Pcrit_slide_width</strong>: sliding bin width based on PO2. Pcrit defined as breakpoint.</p>
<p><strong>Pcrit_on_duration</strong>: breakpoint Pcrit using PO2 vs duration (biased low).</p>
<p><strong>Pcrit_sub_reg_corrected</strong>: Plate Reader only - applies to runs where Pcrit was adjusted to compensate for the poor factory calibration of sensor dots.</p>
<p><strong>BCO-DMO Processing:</strong><br />
- replaced "NA" with "nd" (no data);<br />
- renamed fields to comply with BCO-DMO naming conventions;<br />
- converted date to YYYY-MM-DD format;<br />
- changed year of 2027 to 2017 on line 286;<br />
- replaced values of "15? 18?" in the Temp column with "nd".</p></div>
855732
Zooplankton metabolic traits
2021-07-15T12:33:02-04:00
2021-07-15T12:33:02-04:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:855732
Respirometry data for pelagic crustaceans, cephalopods, and fish collected on R/V Sikuliaq cruise SKQ201701S from January to February 2017
This dataset comprises results of respirometry experiments conducted at sea using pelagic crustaceans, cephalopods, and fish collected on R/V Sikuliaq cruise SKQ201701S from January to February 2017. Experimental animals were collected using either a modified tucker trawl or MOCNESS.
false
Seibel, B., Roman, C., Wishner, K. (2021) Respirometry data for pelagic crustaceans, cephalopods, and fish collected on R/V Sikuliaq cruise SKQ201701S from January to February 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-07-22 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.855732.1 [access date]
true
1
10.26008/1912/bco-dmo.855732.1
false
2021-07-22
Datapackage.json
Frictionless Data Package
https://www.bco-dmo.org/dataset/855732/datapackage.json
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PDF
https://www.bco-dmo.org/dataset/855732/Dataset_description.pdf
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ISO 19115-2 (NOAA Profile)
https://www.bco-dmo.org/dataset/855732/iso
application/xml
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Dublin Core
https://www.bco-dmo.org/dataset/855732/dublin-core
application/xml
http://purl.org/dc/elements/1.1/
855732
http://lod.bco-dmo.org/id/dataset/855732
2017-01-22 - 2017-02-13
2017-01-22
2017-01-22
---22
21
--01
2017
2017-02-13
2017-02-13
---13
43
--02
2017
OSPREY
http://www.opengis.net/def/crs/OGC/1.3/CRS84
<http://www.opengis.net/def/crs/OGC/1.3/CRS84> POLYGON((-118.05 21.22, -116.5 21.22, -116.5 21.22, -116.5 22.42, -118.05 22.42, -118.05 21.22))
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