http://lod.bco-dmo.org/id/dataset/855966
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2021-07-16
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Major nutrient concentrations in seawater from an incubation experiment carried out in China in April 2011
2021-07-16
publication
2021-07-16
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2021-08-04
publication
https://doi.org/10.26008/1912/bco-dmo.855966.1
Adina Paytan
University of California-Santa Cruz
principalInvestigator
Katherine R.M. Mackey
University of California-Santa Cruz
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Paytan, A., Mackey, K. R. (2021) Major nutrient concentrations in seawater from an incubation experiment carried out in China in April 2011. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-07-16 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.855966.1 [access date]
Seawater Nutrient Concentrations from China incubation experiment, April 2011 Dataset Description: Methods and Sampling: <p>Seawater for incubation experiments was collected at two sites located in and near the Yangtze River plume in April 2011 aboard a local fishing boat. Site 1 was within the plume, and site 2 was further away from the river discharge point. Site 1 was nearer to the mouth of the Yangtze River, between two islands in an area with visible suspended sediment (30° 43′ 04″N, 122° 39′ 58″E). Site 2 was east of the Zhoushan Archipelago in waters influenced by the Kuroshio Current outside the area of high sediment resuspension from the Yangtze River, and had a salinity of 31.7ppt (30° 38′ 12.2″N, 122° 56′ 8.51″ E). Site 1, being more heavily influenced by the river discharge, had a salinity of 28.7ppt. Assuming river water has a salinity of zero and ocean water has a salinity of 33, the water we collected at site 1 was a mixture of 87% seawater and 13% river water, while site 2 was 96% seawater and 4% river water. Both of these sites are clearly affected by the Yangtze River outflow, and this is reflected in their salinity levels and nutrient characteristics.</p>
<p>Trace metal clean techniques were used to collect incubation water and during all handling and sample processing steps. Acid cleaning included a 1 week Citranox soaking step, followed by a 1week 10% hydrochloric acid soaking step, followed by a thorough milliQ rinse. All containers and equipment were stored double bagged until use after acid cleaning. For each experiment, surface seawater was collected using a peristaltic pump with acid cleaned C-Flex tubing, passed through 80 μm mesh into acid clean, sample rinsed 50L carboys, and transported to Shengsi Island in the dark. Transport took less than 2 h. In the lab, water was dispensed into 500 mL acid clean, sample rinsed, clear polycarbonate bottles. Nutrient additions included nitrogen (N, as 10 μM NaNO3 + 0.5 μM (NH4)2SO4), phosphorus (P, as 0.5 μM NaH2PO4), or N + P together, and these concentrations were designed to approximately double the background concentrations of these nutrients observed at site 1. Control bottles contained no nutrient additions.</p>
<p>Aerosol treatments included aerosols representing 5 different dates of collection (see below). To simulate 10 days of a moderately strong deposition event for this region (1 g m−2 d−1) over a 10 m mixed layer, 1 mg aerosol on the filter was added per L of seawater. The aerosol filter was cut to the correct size to deliver the appropriate amount of aerosol and then added directly to the bottles. No prior leaching step was included to increase the solubility of the aerosols. The annual average deposition flux for the region is 4.6–98 g m−2 y−1. The deposition rate assumed for the additions is thus an order of magnitude above average and represents strong wind or dust storm conditions. For aerosol NW37, concentrations of 0.2 mg L−1 (similar to average or typical loading at the site) and 5 mg L− (very high deposition, strong dust storm conditions) were also included to test phytoplankton responses to aerosol dosing. Bottles were incubated in a flowing seawater pool by pumping coastal seawater into the pool at a rate high enough to maintain ambient coastal seawater temperatures without allowing the pool to warm from sunlight. No attempt to artificially manipulate temperature was made. The pool was shaded with a neutral density shade cloth to attenuate sunlight 50% for both site 1 and site 2 experiments. Three bottles per treatment were sampled at "time zero" (collected immediately after nutrient or aerosol additions were made) and on the final day of the incubation 3 days later. The incubation time of 3 days was selected because it is long enough to observe changes in phytoplankton community structure based on prior studies, while avoiding major bottle effects that occur with longer-term incubation experiments.</p>
<p>Water samples for nutrient analyses from the incubation experiments were filtered (0.2μm PES) in a laminar flow hood following trace metal clean techniques. Sample bottles were stored in clean plastic bags and stored frozen. Nitrate (plus nitrite) and phosphate were analyzed via a Lachat Autoanalyzer as described previously (Mackey K. R. et al., 2012) using standards prepared in low nutrient seawater. Detection limits were determined as three times the standard deviation of the blank (5 replicates of low nutrient seawater), and were 0.28μM for nitrate and 0.005 μM for phosphate.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0850467 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0850467
completed
Adina Paytan
University of California-Santa Cruz
831-459-1437
Earth & Marine Sciences C308 1156 High St
Santa Cruz
CA
95064
USA
apaytan@ucsc.edu
pointOfContact
Katherine R.M. Mackey
University of California-Santa Cruz
949-824-1133
3204 Croul Hall
Irvine
CA
92697
USA
kmackey@uci.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Site
Latitude
Longitude
Treatment
NO3_from_additions
PO43_from_additions
NO3_at_time_zero_Concentration
NO3_at_time_zero_Standard_error
PO43_at_time_zero_Concentration
PO43_at_time_zero_Standard_error
Lachat Autoanalyzer
theme
None, User defined
site
latitude
longitude
treatment
Nitrate
phosphate
featureType
BCO-DMO Standard Parameters
Nutrient Autoanalyzer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Atmospheric Deposition Impacts on Marine Ecosystems
http://pmc.ucsc.edu/~apaytan/page_projects.html
Atmospheric Deposition Impacts on Marine Ecosystems
<p>Chemical components delivered to the surface ocean through atmospheric deposition influence ocean productivity and ecosystem structure thus are tightly related to the global carbon cycle and climate. Accordingly, the major aim of this project is to quantitatively estimate the variable impact of aerosols on marine phytoplankton and to determine the specific effects on various taxa. Such data could in the future be used to better understand the global impact of aerosols on the oceanic ecosystem. To accomplish this goal the PI will monitor aerosol dry deposition fluxes, determine aerosol sources, obtain the chemical composition and solubility of aerosols, and evaluate the contribution of aerosols to nutrient and trace metal budgets of seawater at two oceanographically different sites (Bermuda and Monterey Bay) representing open ocean and coastal setting. The effects of the different aerosol "types" (defined by source and chemical characteristics) on specific phytoplankton taxa will also be evaluated using pure culture and natural samples bioassays. This project is particularly important in light of the role atmospheric deposition can resume in oligotrophic and coastal settings and the predicted future global conditions of increased aridity and urbanization and associated changes in dust fluxes and composition.</p>
ADIMA
largerWorkCitation
project
eng; USA
oceans
122.666
122.936
30.6367
30.7178
2011-04-01
2011-04-30
Gulf of Aqaba, Atlantic Ocean (Bermuda Time Series Station), Monterey Bay
0
BCO-DMO catalogue of parameters from Major nutrient concentrations in seawater from an incubation experiment carried out in China in April 2011
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/855987.rdf
Name: Site
Units: unitless
Description: <p>Location/site number</p>
http://lod.bco-dmo.org/id/dataset-parameter/855988.rdf
Name: Latitude
Units: decimal degrees North
Description: <p>Site latitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/855989.rdf
Name: Longitude
Units: decimal degrees East
Description: <p>Site longitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/855990.rdf
Name: Treatment
Units: unitless
Description: <p>treatment in the incubation experiment:<br />
<strong>Control</strong>: Control bottles contained no nutrient additions</p>
<p><strong>N</strong>: nitrogen addition (N, as 10 μM NaNO3 + 0.5 μM (NH4)2SO4)</p>
<p><strong>P</strong>: phosphorus addition (P, as 0.5 μM NaH2PO4)</p>
<p><strong>N+P</strong>: N+P addition together</p>
<p><strong>NW32</strong>: One of the aerosol treatments. 1 mg aerosol on the MW32 (collected 19 Nov 2010) filter was added per L of seawater. MW: northwestern (NW) back trajectories originating over mainland China</p>
<p><strong>NW34</strong>: One of the aerosol treatments. 1 mg aerosol on the MW34 filter (collected 23 Nov 2010) was added per L of seawater. MW: northwestern (NW) back trajectories originating over mainland China</p>
<p><strong>NW37</strong>: One of the aerosol treatments. 1 mg aerosol on the MW37 filter (collected 29 Nov 2010) was added per L of seawater. MW: northwestern (NW) back trajectories originating over mainland China</p>
<p><strong>PO19</strong>: One of the aerosol treatments. 1 mg aerosol on the PO19 filter (collected 16 Aug 2010) was added per L of seawater. PO: back trajectories originating over the Pacific Ocean</p>
<p><strong>PO24</strong>: One of the aerosol treatments. 1 mg aerosol on the PO24 filter (collected 26 Aug 2010) was added per L of seawater. PO: back trajectories originating over the Pacific Ocean</p>
http://lod.bco-dmo.org/id/dataset-parameter/855991.rdf
Name: NO3_from_additions
Units: micromolar (uM)
Description: <p>The amount of nitrate added to the incubation container</p>
http://lod.bco-dmo.org/id/dataset-parameter/855992.rdf
Name: PO43_from_additions
Units: micromolar (uM)
Description: <p>The amount of phosphate added to the incubation container</p>
http://lod.bco-dmo.org/id/dataset-parameter/855993.rdf
Name: NO3_at_time_zero_Concentration
Units: micromolar (uM)
Description: <p>Mean nitrate concentration in the beginning of experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/855994.rdf
Name: NO3_at_time_zero_Standard_error
Units: micromolar (uM)
Description: <p>Standard error of nitrate concentrations in the beginning of experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/855995.rdf
Name: PO43_at_time_zero_Concentration
Units: micromolar (uM)
Description: <p>Mean phosphate concentration in the beginning of experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/855996.rdf
Name: PO43_at_time_zero_Standard_error
Units: micromolar (uM)
Description: <p>Standard error of phosphate concentrations in the beginning of experiment</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
1197
https://darchive.mblwhoilibrary.org/bitstream/1912/27402/1/dataset-855966_seawater-nutrient-concentrations-china-incubation-experiment-april-2011__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.855966.1
download
onLine
dataset
<p>Seawater for incubation experiments was collected at two sites located in and near the Yangtze River plume in April 2011 aboard a local fishing boat. Site 1 was within the plume, and site 2 was further away from the river discharge point. Site 1 was nearer to the mouth of the Yangtze River, between two islands in an area with visible suspended sediment (30° 43′ 04″N, 122° 39′ 58″E). Site 2 was east of the Zhoushan Archipelago in waters influenced by the Kuroshio Current outside the area of high sediment resuspension from the Yangtze River, and had a salinity of 31.7ppt (30° 38′ 12.2″N, 122° 56′ 8.51″ E). Site 1, being more heavily influenced by the river discharge, had a salinity of 28.7ppt. Assuming river water has a salinity of zero and ocean water has a salinity of 33, the water we collected at site 1 was a mixture of 87% seawater and 13% river water, while site 2 was 96% seawater and 4% river water. Both of these sites are clearly affected by the Yangtze River outflow, and this is reflected in their salinity levels and nutrient characteristics.</p>
<p>Trace metal clean techniques were used to collect incubation water and during all handling and sample processing steps. Acid cleaning included a 1 week Citranox soaking step, followed by a 1week 10% hydrochloric acid soaking step, followed by a thorough milliQ rinse. All containers and equipment were stored double bagged until use after acid cleaning. For each experiment, surface seawater was collected using a peristaltic pump with acid cleaned C-Flex tubing, passed through 80 μm mesh into acid clean, sample rinsed 50L carboys, and transported to Shengsi Island in the dark. Transport took less than 2 h. In the lab, water was dispensed into 500 mL acid clean, sample rinsed, clear polycarbonate bottles. Nutrient additions included nitrogen (N, as 10 μM NaNO3 + 0.5 μM (NH4)2SO4), phosphorus (P, as 0.5 μM NaH2PO4), or N + P together, and these concentrations were designed to approximately double the background concentrations of these nutrients observed at site 1. Control bottles contained no nutrient additions.</p>
<p>Aerosol treatments included aerosols representing 5 different dates of collection (see below). To simulate 10 days of a moderately strong deposition event for this region (1 g m−2 d−1) over a 10 m mixed layer, 1 mg aerosol on the filter was added per L of seawater. The aerosol filter was cut to the correct size to deliver the appropriate amount of aerosol and then added directly to the bottles. No prior leaching step was included to increase the solubility of the aerosols. The annual average deposition flux for the region is 4.6–98 g m−2 y−1. The deposition rate assumed for the additions is thus an order of magnitude above average and represents strong wind or dust storm conditions. For aerosol NW37, concentrations of 0.2 mg L−1 (similar to average or typical loading at the site) and 5 mg L− (very high deposition, strong dust storm conditions) were also included to test phytoplankton responses to aerosol dosing. Bottles were incubated in a flowing seawater pool by pumping coastal seawater into the pool at a rate high enough to maintain ambient coastal seawater temperatures without allowing the pool to warm from sunlight. No attempt to artificially manipulate temperature was made. The pool was shaded with a neutral density shade cloth to attenuate sunlight 50% for both site 1 and site 2 experiments. Three bottles per treatment were sampled at "time zero" (collected immediately after nutrient or aerosol additions were made) and on the final day of the incubation 3 days later. The incubation time of 3 days was selected because it is long enough to observe changes in phytoplankton community structure based on prior studies, while avoiding major bottle effects that occur with longer-term incubation experiments.</p>
<p>Water samples for nutrient analyses from the incubation experiments were filtered (0.2μm PES) in a laminar flow hood following trace metal clean techniques. Sample bottles were stored in clean plastic bags and stored frozen. Nitrate (plus nitrite) and phosphate were analyzed via a Lachat Autoanalyzer as described previously (Mackey K. R. et al., 2012) using standards prepared in low nutrient seawater. Detection limits were determined as three times the standard deviation of the blank (5 replicates of low nutrient seawater), and were 0.28μM for nitrate and 0.005 μM for phosphate.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing:</strong><br />
- split the original Location column into Site, Latitude, and Longitude;<br />
- converted latitude and longitude to decimal degrees from degrees, minutes, seconds;<br />
- renamed fields to comply with BCO-DMO naming conventions.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Lachat Autoanalyzer
Lachat Autoanalyzer
PI Supplied Instrument Name: Lachat Autoanalyzer Instrument Name: Nutrient Autoanalyzer Instrument Short Name:Nutrient Autoanalyzer Instrument Description: Nutrient Autoanalyzer is a generic term used when specific type, make and model were not specified. In general, a Nutrient Autoanalyzer is an automated flow-thru system for doing nutrient analysis (nitrate, ammonium, orthophosphate, and silicate) on seawater samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/