<div><p>See complete methodology in Franzè and Menden-Deuer, 2020.</p>
<p>Clonal cultures of Oxyrrhis marina (CCMP 3375), Gyrodinium dominans (SPMC 103), and Protoperidinium bipes were established from single-cell isolation. Herbivorous protists were acclimated to each target temperature before growth rates were measured. Due to the wide temperature range tested (0−22°C), cultures were moved from the initial temperature of 15°C through gradual transitions limited to at most 3°C. The experimental Day 0 (D0) was defined as the first day on which acclimated growth rates were measured. To determine predator and prey abundance, samples were preserved in acid Lugol at a 2% final concentration (Menden-Deuer et al. 2001) and enumerated using a Sedgewick-Rafter slide (1 ml volume) and a Nikon Eclipse E800 light microscope equipped with phase contrast. Herbivore biovolume was calculated based on linear dimensions obtained from ≥35 cells measured at each time point and temperature treatment using an image analysis system consisting of a high-resolution digital camera (Allied Vision, Stingray F45) and ImageJ software (version 1.5i).</p>
<p>Growth rates of the 3 herbivorous species were determined at 7 incubation temperatures: 0, 2, 5, 10, 15, 18, and 22°C. Changes in O. marina, G. dominans, and P. bipes abundance and biomass were determined over 24 h incubations of the triplicate 150 ml bottles and used to calculate growth rates (μ d−1) assuming exponential growth: μ = ln(Nt/N0)/t, where: N0 and Nt are the initial and final cell abundance (or biomass) respectively, and t is the experiment duration in days. Measurements were made at Day 0 and again after 10 and 30 days.</p></div>
Comparison of abundance-based growth rate predictions
Comparison of abundance-based growth rate predictions
<div><p>Statistical analyses were performed using R (version 1.2.1335) and Prism 7.</p>
<p>BCO-DMO Processing:<br />
- renamed fields to comply with BCO-DMO naming conventions.</p></div>
857356
Comparison of abundance-based growth rate predictions
2021-08-05T12:21:15-04:00
2021-08-05T12:21:15-04:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:857356
Comparison of abundance-based growth rate predicted following Q10 model, Eppley’s equation, and the linear model obtained in Franzè and Menden-Deuer, 2020
This dataset contains a comparison of abundance-based growth rate predicted following Q10 model, Eppley’s equation, and the linear model obtained in Franzè and Menden-Deuer, 2020 (doi: 10.3354/meps13200). The data provided served for the production of Figure 6 of Franzè and Menden-Deuer, 2020.
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Franzè, G., Menden-Deuer, S. (2021) Comparison of abundance-based growth rate predicted following Q10 model, Eppley’s equation, and the linear model obtained in Franzè and Menden-Deuer, 2020. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-08-05 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.857356.1 [access date]
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10.26008/1912/bco-dmo.857356.1
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2021-08-05
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