http://lod.bco-dmo.org/id/dataset/857356
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2021-08-05
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Comparison of abundance-based growth rate predicted following Q10 model, Eppley’s equation, and the linear model obtained in Franzè and Menden-Deuer, 2020
2021-08-05
publication
2021-08-05
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2021-08-24
publication
https://doi.org/10.26008/1912/bco-dmo.857356.1
Susanne Menden-Deuer
University of Rhode Island
principalInvestigator
Gayantonia Franzè
University of Rhode Island
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Franzè, G., Menden-Deuer, S. (2021) Comparison of abundance-based growth rate predicted following Q10 model, Eppley’s equation, and the linear model obtained in Franzè and Menden-Deuer, 2020. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-08-05 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.857356.1 [access date]
Comparison of abundance-based growth rate predictions Dataset Description: Methods and Sampling: <p>See complete methodology in Franzè and Menden-Deuer, 2020.</p>
<p>Clonal cultures of Oxyrrhis marina (CCMP 3375), Gyrodinium dominans (SPMC 103), and Protoperidinium bipes were established from single-cell isolation. Herbivorous protists were acclimated to each target temperature before growth rates were measured. Due to the wide temperature range tested (0−22°C), cultures were moved from the initial temperature of 15°C through gradual transitions limited to at most 3°C. The experimental Day 0 (D0) was defined as the first day on which acclimated growth rates were measured. To determine predator and prey abundance, samples were preserved in acid Lugol at a 2% final concentration (Menden-Deuer et al. 2001) and enumerated using a Sedgewick-Rafter slide (1 ml volume) and a Nikon Eclipse E800 light microscope equipped with phase contrast. Herbivore biovolume was calculated based on linear dimensions obtained from ≥35 cells measured at each time point and temperature treatment using an image analysis system consisting of a high-resolution digital camera (Allied Vision, Stingray F45) and ImageJ software (version 1.5i).</p>
<p>Growth rates of the 3 herbivorous species were determined at 7 incubation temperatures: 0, 2, 5, 10, 15, 18, and 22°C. Changes in O. marina, G. dominans, and P. bipes abundance and biomass were determined over 24 h incubations of the triplicate 150 ml bottles and used to calculate growth rates (μ d−1) assuming exponential growth: μ = ln(Nt/N0)/t, where: N0 and Nt are the initial and final cell abundance (or biomass) respectively, and t is the experiment duration in days. Measurements were made at Day 0 and again after 10 and 30 days.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1736635 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1736635
completed
Susanne Menden-Deuer
University of Rhode Island
215 South Ferry Rd.
Narragansett
RI
02882
USA
smenden@uri.edu
pointOfContact
Gayantonia Franzè
University of Rhode Island
franze@hi.no
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Temperature
Equation_applied
Estimated_Growth_rate
Allied Vision, Stingray F45
I-36LLVL Series, Percival Scientific
Nikon Eclipse E800 light microscope
theme
None, User defined
water temperature at measurement depth
sampling_method
growth
featureType
BCO-DMO Standard Parameters
Camera
In-situ incubator
Microscope - Optical
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Quantifying Temperature Dependence In Growth & Grazing Rates of Planktonic Herbivores
https://osprey.bco-dmo.org/project/739232
Quantifying Temperature Dependence In Growth & Grazing Rates of Planktonic Herbivores
<p><em>NSF Award Abstract:</em><br />
Plankton, single-celled organisms that inhabit the world's oceans are responsible for the generation of oxygen, cycling energy and matter between the atmosphere and the deep ocean and are the basis for virtually all seafood harvested. These life-giving functions critically depend on the relative rates at which plankton grow and get eaten. How temperature influences those rates is essential to understand plankton responses to environmental changes and ocean dynamics. It is well established that plankton grow faster when temperatures are higher however, whether feeding has a similar temperature dependence is unknown. That means oceanographers are missing key data required to build global predictive models. This project will fill essential knowledge gaps and measure physiological rates of singled celled zooplankton across temperature gradients representing the global ocean, from polar to tropical regions and throughout the seasonal cycle. Researchers will combine laboratory experiments with specimens taken from the coastal ocean (Narragansett Bay), which is exemplary in its strong seasonal temperature variations. These data will provide a clear picture of the production capacity and activity of plankton in a global and dynamic ocean. The project supports an early career scientist, as well as graduate and undergraduate students. Scientists will continue communicating their research to the public through large-scale outreach events, education at the high-school level, and engagement through online and other media. Moreover, researchers will continue collaborating with the Metcalf Institute for Marine & Environmental Reporting to support their Annual Science Immersion Workshop for Journalists and their ongoing work to disseminate research findings through web-based seminars.</p>
<p>Grazing is the single largest loss factor of marine primary production and thus affects a key transfer rate between global organic and inorganic matter pools. Remarkably, data for herbivorous protist growth and grazing rates at temperatures representative of the vast polar regions and during winter and spring periods are extremely sparse. By combining laboratory experiments with ground truthing fieldwork, this project alleviates a central knowledge gap in oceanography and delivers the empirical measurements necessary to derive algorithms to incorporate temperature dependence of heterotrophic protist growth and grazing rates into biogeochemical models. The extraordinary seasonal temperature fluctuations in a temperate coastal estuary (Narragansett Bay) are exploited to measure rates of heterotrophic protists isolated from different temperatures and seasons and to quantify the temperature and acclimation responses of these ecotypes. This project delivers data urgently needed to solve the conundrum of whether herbivorous growth and predation is depressed at low temperatures, implying low trophic transfer rates and high carbon export, or if predation proceeds at rates comparable to temperate systems with primary production largely lost to predation. Large temperature gradients in the global ocean mean that cross-biome and biogeochemical models are particularly sensitive to assumptions about the temperature dependence in modeled rate processes. Establishment of the dependence of heterotrophic plankton physiological rates (growth and grazing) to gradients of temperature, mimicking realistic conditions experienced by plankton in a changing ocean, is a key step towards integrating much needed biological information in biogeochemical modeling efforts. This project makes a significant contribution to linking ecological research with ecosystem models by providing empirically rooted algorithms of the temperature dependence of protistan herbivory and growth rates, key processes in the transformation of organic matter in global biogeochemical cycles and tools critically missing in ecosystem models.</p>
Planktonic Herbivore Temp Dependence
largerWorkCitation
project
eng; USA
oceans
-71.422211
-71.422211
41.492779
41.492779
2017-04-01
2017-06-30
Narragansett Bay
0
BCO-DMO catalogue of parameters from Comparison of abundance-based growth rate predicted following Q10 model, Eppley’s equation, and the linear model obtained in Franzè and Menden-Deuer, 2020
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/857366.rdf
Name: Temperature
Units: degrees Celsius
Description: <p>Theoretical growth temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/857367.rdf
Name: Equation_applied
Units: unitless
Description: <p>Equation applied to predict growth</p>
http://lod.bco-dmo.org/id/dataset-parameter/857368.rdf
Name: Estimated_Growth_rate
Units: per day
Description: <p>Estimated growth rate at target temperature based on the specified equation</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
673
https://darchive.mblwhoilibrary.org/bitstream/1912/27470/1/dataset-857356_comparison-abundance-based-growth-rate-predictions__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.857356.1
download
onLine
dataset
<p>See complete methodology in Franzè and Menden-Deuer, 2020.</p>
<p>Clonal cultures of Oxyrrhis marina (CCMP 3375), Gyrodinium dominans (SPMC 103), and Protoperidinium bipes were established from single-cell isolation. Herbivorous protists were acclimated to each target temperature before growth rates were measured. Due to the wide temperature range tested (0−22°C), cultures were moved from the initial temperature of 15°C through gradual transitions limited to at most 3°C. The experimental Day 0 (D0) was defined as the first day on which acclimated growth rates were measured. To determine predator and prey abundance, samples were preserved in acid Lugol at a 2% final concentration (Menden-Deuer et al. 2001) and enumerated using a Sedgewick-Rafter slide (1 ml volume) and a Nikon Eclipse E800 light microscope equipped with phase contrast. Herbivore biovolume was calculated based on linear dimensions obtained from ≥35 cells measured at each time point and temperature treatment using an image analysis system consisting of a high-resolution digital camera (Allied Vision, Stingray F45) and ImageJ software (version 1.5i).</p>
<p>Growth rates of the 3 herbivorous species were determined at 7 incubation temperatures: 0, 2, 5, 10, 15, 18, and 22°C. Changes in O. marina, G. dominans, and P. bipes abundance and biomass were determined over 24 h incubations of the triplicate 150 ml bottles and used to calculate growth rates (μ d−1) assuming exponential growth: μ = ln(Nt/N0)/t, where: N0 and Nt are the initial and final cell abundance (or biomass) respectively, and t is the experiment duration in days. Measurements were made at Day 0 and again after 10 and 30 days.</p>
Specified by the Principal Investigator(s)
<p>Statistical analyses were performed using R (version 1.2.1335) and Prism 7.</p>
<p>BCO-DMO Processing:<br />
- renamed fields to comply with BCO-DMO naming conventions.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Allied Vision, Stingray F45
Allied Vision, Stingray F45
PI Supplied Instrument Name: Allied Vision, Stingray F45 PI Supplied Instrument Description:Cell measurements were performed using a high-resolution digital camera (Allied Vision, Stingray F45) and ImageJ software (version 1.5i). Instrument Name: Camera Instrument Short Name:camera Instrument Description: All types of photographic equipment including stills, video, film and digital systems. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/311/
I-36LLVL Series, Percival Scientific
I-36LLVL Series, Percival Scientific
PI Supplied Instrument Name: I-36LLVL Series, Percival Scientific PI Supplied Instrument Description:Experiments were conducted using temperature-controlled incubators (I-36LLVL Series, Percival Scientific). Instrument Name: In-situ incubator Instrument Short Name:in-situ incubator Instrument Description: A device on a ship or in the laboratory that holds water samples under controlled conditions of temperature and possibly illumination. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/82/
Nikon Eclipse E800 light microscope
Nikon Eclipse E800 light microscope
PI Supplied Instrument Name: Nikon Eclipse E800 light microscope PI Supplied Instrument Description:Microscopy counts were performed with a Nikon Eclipse E800 light microscope. Instrument Name: Microscope - Optical Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope". Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/