http://lod.bco-dmo.org/id/dataset/858081 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2021-08-10 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Temperature data measured during two experiments testing the effects of acute and chronic thermal stress on the scleractinian coral, Stylophora pistillata 2021-08-10 publication 2021-08-10 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2021-08-11 publication https://doi.org/10.26008/1912/bco-dmo.858081.1 Daniel J. Barshis Old Dominion University principalInvestigator Mark E. Warner University of Delaware principalInvestigator Nicolas R. Evensen Old Dominion University principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Evensen, N. R., Warner, M. E., Barshis, D. J. (2021) Temperature data measured during two experiments testing the effects of acute and chronic thermal stress on the scleractinian coral, Stylophora pistillata. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-08-10 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.858081.1 [access date] Temperature Dataset Description: Methods and Sampling: &lt;p&gt;Two experiments were conducted to test the effects of acute (18 h) and chronic (14 d) thermal stress on the scleractinian coral, &lt;em&gt;Stylophora pistillata&lt;/em&gt;. A full description of the methodology is detailed in the published manuscript Evensen et al. (2021).&lt;/p&gt; &lt;p&gt;Multiple ramets of 8 &lt;em&gt;Stylophora pistillata&lt;/em&gt; genets were used in two experiments: an 18 h acute thermal stress assay using the Coral Bleaching Automated Stress System (CBASS) and a 14 day chronic heat stress experiment using the Red Sea Simulator (RSS).&amp;nbsp;Experiments&amp;nbsp;were performed in Eilat, Israel in January-February 2019.&amp;nbsp;&lt;/p&gt; &lt;p&gt;In each experiment, corals were exposed to four temperature treatments (27°C (summer ambient), 29.5°C, 32°C, and 34.5°C), with physiological responses of the coral and symbionts assessed at the end of the thermal stress exposure (T1) and after a period of recovery (T2). This dataset&amp;nbsp;represents the temperature data measured during&amp;nbsp;the acute (CBASS) and chronic heat (RSS) stress experiments.&amp;nbsp;&lt;/p&gt; &lt;p&gt;Physiological responses of the coral host and associated endosymbionts were measured at the end of the heating hold (after 6 h in the CBASS and 6-10 days in the RSS), referred to as T1, and after a recovery period (12 h and 3-7 days post-heat stress in the CBASS and&amp;nbsp; RSS, respectively), referred to as T2.&amp;nbsp;Control/baseline samples were also collected for each genet - a 'field'&amp;nbsp;sample was assessed upon returning from sample collection and a 'T0' sample was assessed after ~ 2 h incubation in the experimental tanks, immediately prior to the start of the experiments, during which time seawater temperatures in the tanks was steadily ramped up from 22°C to 27°C.&amp;nbsp;Experimental tanks were then ramped up to temperature treatments reaching 27°C, 29.5°C, 32°C, and 34.5°C, with each temperature containing two replicate tanks (A and B).&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Problem report&lt;/strong&gt;:&lt;br /&gt; Missing data were samples that were not measured or that are missing due to sample mortality.&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1833215 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1833215 Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1833201 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1833201 completed Daniel J. Barshis Old Dominion University 757-683-3614 Dept of Biological Sciences MGB 110 Norfolk VA 23529-0266 US dbarshis@odu.edu pointOfContact Mark E. Warner University of Delaware 302-645-4365 School of Marine Science and Policy 700 Pilottown Rd. Lewes DE 19958 USA mwarner@udel.edu pointOfContact Nicolas R. Evensen Old Dominion University Department of Biological Sciences Norfolk VA 23529 USA nevensen@odu.edu pointOfContact asNeeded Dataset Version: 1 Unknown Experiment DateTime Tank Temperature IceProbe Thermoelectric chillers CBASS MACSQuant Analyzer 10, Miltenyi Biotec Diax 900, Heidolph Instruments GalaxyHydro, Roleandro LI-192, Li-COR FireSting O2, Pyroscience oxygen probes Biotek HT Synergy plate reader theme None, User defined experiment type ISO_DateTime_Local tank water temperature at measurement depth featureType BCO-DMO Standard Parameters Aquarium Aquarium chiller Centrifuge Coral Bleaching Automated Stress System Flow Cytometer Homogenizer LED light LI-COR LI-192 PAR Sensor Oxygen Sensor plate reader instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. EAGER: Collaborative Research: Bleaching phenotypes of acute vs. chronic coral bleaching susceptibility and resilience: towards a standardized coral resilience diagnostic https://osprey.bco-dmo.org/project/776327 EAGER: Collaborative Research: Bleaching phenotypes of acute vs. chronic coral bleaching susceptibility and resilience: towards a standardized coral resilience diagnostic <p><em>NSF Award Abstract:</em><br /> The past few years have seen an unprecedented amount of coral bleaching across the globe. Global bleaching events in 2015-17, severely impacting iconic coral reefs in places such as the Great Barrier Reef, Micronesia, Hawaiian Islands, and Caribbean, were the worst recorded in recent human history. When ocean temperatures rise, the symbiosis between reef-building corals and their photosynthetic algae deteriorates, many times resulting in widespread coral die-offs as corals can starve without their symbiotic partners to supply food. These widespread events can have drastic impacts on ocean health and biodiversity, as well as the communities that depend on reefs for fishing, tourism, and protection from storms. Importantly, some corals resist or recover from bleaching better than others. Such variability in coral response to ocean warming could be critical to reef survival in the future, yet the scientific community lacks any standardized diagnostics to rapidly assess bleaching tolerance limits. Here, we plan to: 1) develop a standardized, short-term exposure to assess bleaching limits (analogous to cardiac stress tests for humans), 2) design an experimental system capable of delivering a range of thermal treatments as an open-source, low-cost, highly-portable device that can be readily adapted for bleaching tests in a wide variety of coral habitats, and 3) disseminate the results, instructions, and technologies to the reef research and conservation community through a combination of hands-on workshops, online outreach materials, press releases, and open-access research publications. Widespread dissemination of project products will be achieved via hands-on demonstrations and workshops in key geographic areas (Middle East, Caribbean, and Indo-Pacific), with a focus on the assembly of the system and operation of the experimental assay using local corals. This project will train both graduate students and a postdoctoral researcher, and brings together a team of national and global researchers in a collaborative investigation to address the international problem of coral bleaching.</p> <p>With each passing year, coral bleaching has shifted from an issue of serious sporadic concern to a critical widespread threat to reefs across the globe that is increasing in frequency and severity. However, during widespread bleaching events, some scattered corals and reef sections are able to survive better than others. Whether this is due to acclimatization or adaptation in thermal stress tolerance, this variability in response is critical to coral resilience to climate impacts. Currently, the scientific community lacks a standardized approach to rapidly assess coral thermal limits and identify resilient individuals or populations. Present day approaches range from observational surveys of natural bleaching and mortality, to multiple weeks of controlled chronic thermal exposure, to rapid, single or multi-day acute heat shocks. To what degree bleaching response varies across short-term versus longer-term experiments and how these responses compare to natural bleaching patterns is largely unknown. Using a group of coral species representative of a historical range of bleaching susceptibility (e.g., Acropora hemprichii, Pocillopora meandrina, and Porites lobata), research will address this important knowledge gap by experimental evaluation of the bleaching response to acute (0 - 2 day) versus chronic (&gt;4 week) thermal stress. The overarching questions for this study are: how are the acute and chronic coral bleaching responses related, and can investigators predict ecologically relevant bleaching outcomes from the response to a short-term, acute heat-stress? To answer these questions, the research team will: 1) objectively compare acute versus chronic heat-stress exposures and synthesize a variety of response metrics based on core physiological measurements to develop a standardized, short-term thermal assay and diagnostic approach to rapidly assess bleaching, 2) operationalize an experimental system built around an open-source, cost-effective, easily transportable temperature control technology, and 3) distribute the results, experimental procedures, and temperature controlling technologies to the reef research and conservation communities. This project will produce an affordable experimental system and short-term diagnostic capable of determining coral thermal limits in just a few days in almost any location with reliable access to seawater and electricity or a portable generator. The research fills a critical knowledge gap through the development of a standardized set of diagnostic tools to assess coral thermal vulnerability before widespread bleaching events actually occur, so that proactive conservation and management strategies can be implemented ahead of widespread impacts to reef ecosystems.</p> <p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p> EAGER-CBASS largerWorkCitation project eng; USA oceans 34.951579 34.951579 29.55723 29.55723 2019-01-20 2019-02-03 Red Sea, Thuwal, Saudi Arabia, Eilat Israel 0 BCO-DMO catalogue of parameters from Temperature data measured during two experiments testing the effects of acute and chronic thermal stress on the scleractinian coral, Stylophora pistillata Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/858107.rdf Name: Experiment Units: unitless Description: <p>experiment: CBASS = Coral Bleaching Automated Stress System; RSS = Red Sea Simulator</p> http://lod.bco-dmo.org/id/dataset-parameter/858108.rdf Name: DateTime Units: unitless Description: <p>date and time (local time zone: UTC/GMT+3:00); format: YYYY-MM-DDThh:mm</p> http://lod.bco-dmo.org/id/dataset-parameter/858109.rdf Name: Tank Units: unitless Description: <p>tank ID</p> http://lod.bco-dmo.org/id/dataset-parameter/858110.rdf Name: Temperature Units: degrees Celsius Description: <p>temperature; RSS temperatures are averaged over 1 h and CBASS temperatures are averaged over 15 mins</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 102087 https://darchive.mblwhoilibrary.org/bitstream/1912/27434/1/dataset-858081_temperature__v1.tsv download https://doi.org/10.26008/1912/bco-dmo.858081.1 download onLine dataset &lt;p&gt;Two experiments were conducted to test the effects of acute (18 h) and chronic (14 d) thermal stress on the scleractinian coral, &lt;em&gt;Stylophora pistillata&lt;/em&gt;. A full description of the methodology is detailed in the published manuscript Evensen et al. (2021).&lt;/p&gt; &lt;p&gt;Multiple ramets of 8 &lt;em&gt;Stylophora pistillata&lt;/em&gt; genets were used in two experiments: an 18 h acute thermal stress assay using the Coral Bleaching Automated Stress System (CBASS) and a 14 day chronic heat stress experiment using the Red Sea Simulator (RSS).&amp;nbsp;Experiments&amp;nbsp;were performed in Eilat, Israel in January-February 2019.&amp;nbsp;&lt;/p&gt; &lt;p&gt;In each experiment, corals were exposed to four temperature treatments (27°C (summer ambient), 29.5°C, 32°C, and 34.5°C), with physiological responses of the coral and symbionts assessed at the end of the thermal stress exposure (T1) and after a period of recovery (T2). This dataset&amp;nbsp;represents the temperature data measured during&amp;nbsp;the acute (CBASS) and chronic heat (RSS) stress experiments.&amp;nbsp;&lt;/p&gt; &lt;p&gt;Physiological responses of the coral host and associated endosymbionts were measured at the end of the heating hold (after 6 h in the CBASS and 6-10 days in the RSS), referred to as T1, and after a recovery period (12 h and 3-7 days post-heat stress in the CBASS and&amp;nbsp; RSS, respectively), referred to as T2.&amp;nbsp;Control/baseline samples were also collected for each genet - a 'field'&amp;nbsp;sample was assessed upon returning from sample collection and a 'T0' sample was assessed after ~ 2 h incubation in the experimental tanks, immediately prior to the start of the experiments, during which time seawater temperatures in the tanks was steadily ramped up from 22°C to 27°C.&amp;nbsp;Experimental tanks were then ramped up to temperature treatments reaching 27°C, 29.5°C, 32°C, and 34.5°C, with each temperature containing two replicate tanks (A and B).&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Problem report&lt;/strong&gt;:&lt;br /&gt; Missing data were samples that were not measured or that are missing due to sample mortality.&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;&lt;strong&gt;Data Processing:&amp;nbsp;&lt;/strong&gt;&lt;br /&gt; Data were organized and plotted using R statistical software (version 4.0.3) or in Prism (v. 8.0; GraphPad Software, Inc.).&lt;/p&gt; &lt;p&gt;&lt;strong&gt;BCO-DMO Processing:&lt;/strong&gt;&lt;br /&gt; - renamed fields to comply with BCO-DMO naming conventions;&lt;br /&gt; - changed date/time field to ISO8601 format.&lt;/p&gt; &lt;p&gt;The originally submitted GitHub repository&amp;nbsp;&lt;a href=&quot;https://github.com/BarshisLab/CBASS-vs-RSS-Physiology&quot; target=&quot;_blank&quot;&gt;https://github.com/BarshisLab/CBASS-vs-RSS-Physiology&lt;/a&gt; was&amp;nbsp;forked to &lt;a href=&quot;https://github.com/BCODMO/CBASS-vs-RSS-Physiology&quot; target=&quot;_blank&quot;&gt;https://github.com/BCODMO/CBASS-vs-RSS-Physiology&lt;/a&gt; and tagged with release 1.0,&amp;nbsp;which corresponds with this dataset submission. The original repository may have continued updates.&lt;/p&gt; Specified by the Principal Investigator(s) asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact PI Supplied Instrument Name: Instrument Name: Aquarium Instrument Short Name:Aquarium Instrument Description: Aquarium - a vivarium consisting of at least one transparent side in which water-dwelling plants or animals are kept IceProbe Thermoelectric chillers IceProbe Thermoelectric chillers PI Supplied Instrument Name: IceProbe Thermoelectric chillers PI Supplied Instrument Description:Temperature control in each tank is achieved using a combination of IceProbe Thermoelectric chillers (Nova Tec) and 150-200 W aquarium heaters linked to a custom-built controller (Arduino MEGA 2560). Instrument Name: Aquarium chiller Instrument Short Name:Aquarium chiller Instrument Description: Immersible or in-line liquid cooling device, usually with temperature control. PI Supplied Instrument Name: Instrument Name: Centrifuge Instrument Short Name: Instrument Description: A machine with a rapidly rotating container that applies centrifugal force to its contents, typically to separate fluids of different densities (e.g., cream from milk) or liquids from solids. CBASS CBASS PI Supplied Instrument Name: CBASS Instrument Name: Coral Bleaching Automated Stress System Instrument Short Name:CBASS Instrument Description: CBASS, which stands for "Coral Bleaching Automated Stress System", are portable, field-deployable experimental tanks used to apply rapid, acute heat stress challenges. This system is described in: Voolstra, C. R., Buitrago‐López, C., Perna, G., Cárdenas, A., Hume, B. C. C., Rädecker, N., & Barshis, D. J. (2020). Standardized short‐term acute heat stress assays resolve historical differences in coral thermotolerance across microhabitat reef sites. Global Change Biology, 26(8), 4328-4343. Portico. https://doi.org/10.1111/gcb.15148 MACSQuant Analyzer 10, Miltenyi Biotec MACSQuant Analyzer 10, Miltenyi Biotec PI Supplied Instrument Name: MACSQuant Analyzer 10, Miltenyi Biotec Instrument Name: Flow Cytometer Instrument Short Name:Flow Cytometer Instrument Description: Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells. (from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/ Diax 900, Heidolph Instruments Diax 900, Heidolph Instruments PI Supplied Instrument Name: Diax 900, Heidolph Instruments Instrument Name: Homogenizer Instrument Short Name:Homogenizer Instrument Description: A homogenizer is a piece of laboratory equipment used for the homogenization of various types of material, such as tissue, plant, food, soil, and many others. GalaxyHydro, Roleandro GalaxyHydro, Roleandro PI Supplied Instrument Name: GalaxyHydro, Roleandro PI Supplied Instrument Description:Tanks were located indoors and received ~ 300 μmol quanta m−2 s−1 from LED aquarium lights (GalaxyHydro, Roleandro) for 12 h per day, as measured via a Li-Cor spherical quantum PAR (Photosynthetically Active Radiation) sensor (LI-192, Li-COR). Instrument Name: LED light Instrument Short Name: Instrument Description: A light-emitting diode (LED) is a semiconductor light source that emits light when current flows through it. Electrons in the semiconductor recombine with electron holes, releasing energy in the form of photons. LI-192, Li-COR LI-192, Li-COR PI Supplied Instrument Name: LI-192, Li-COR PI Supplied Instrument Description:Tanks were located indoors and received ~ 300 μmol quanta m−2 s−1 from LED aquarium lights (GalaxyHydro, Roleandro) for 12 h per day, as measured via a Li-Cor spherical quantum PAR (Photosynthetically Active Radiation) sensor (LI-192, Li-COR). Instrument Name: LI-COR LI-192 PAR Sensor Instrument Short Name:LI-COR LI-192 PAR Instrument Description: The LI-192 Underwater Quantum Sensor (UWQ) measures underwater or atmospheric Photon Flux Density (PPFD) (Photosynthetically Available Radiation from 360 degrees) using a Silicon Photodiode and glass filters encased in a waterproof housing. The LI-192 is cosine corrected and features corrosion resistant, rugged construction for use in freshwater or saltwater and pressures up to 800 psi (5500 kPa, 560 meters depth). Typical output is in um s-1 m-2. The LI-192 uses computer-tailored filter glass to achieve the desired quantum response. Calibration is traceable to NIST. The LI-192 serial numbers begin with UWQ-XXXXX. LI-COR has been producing Underwater Quantum Sensors since 1973. These LI-192 sensors are typically listed as LI-192SA to designate the 2-pin connector on the base of the housing and require an Underwater Cable (LI-COR part number 2222UWB) to connect to the pins on the Sensor and connect to a data recording device. The LI-192 differs from the LI-193 primarily in sensitivity and angular response. 193: Sensitivity: Typically 7 uA per 1000 umol s-1 m-2 in water. Azimuth: < ± 3% error over 360° at 90° from normal axis. Angular Response: < ± 4% error up to ± 90° from normal axis. 192: Sensitivity: Typically 4 uA per 1000 umol s-1 m-2 in water. Azimuth: < ± 1% error over 360° at 45° elevation. Cosine Correction: Optimized for underwater and atmospheric use. (www.licor.com) Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0120/ FireSting O2, Pyroscience oxygen probes FireSting O2, Pyroscience oxygen probes PI Supplied Instrument Name: FireSting O2, Pyroscience oxygen probes PI Supplied Instrument Description:Oxygen flux in the chambers was measured at 1-s intervals using oxygen probes (FireSting O2, Pyroscience). Instrument Name: Oxygen Sensor Instrument Short Name:Dissolved Oxygen Sensor Instrument Description: An electronic device that measures the proportion of oxygen (O2) in the gas or liquid being analyzed Biotek HT Synergy plate reader Biotek HT Synergy plate reader PI Supplied Instrument Name: Biotek HT Synergy plate reader Instrument Name: plate reader Instrument Short Name: Instrument Description: Plate readers (also known as microplate readers) are laboratory instruments designed to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. Sample reactions can be assayed in 6-1536 well format microtiter plates. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 uL per well. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µL per well. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization. From: http://en.wikipedia.org/wiki/Plate_reader, 2014-09-0-23.