19:I[42426,["/_next/static/immutable/chunks/1xctgzqre-wsy.js","/_next/static/immutable/chunks/06_-wz6pdtp2b.js","/_next/static/immutable/chunks/30e52g49hg40p.js","/_next/static/immutable/chunks/02yq6tul2z-oh.js","/_next/static/immutable/chunks/10bx_ec2vj7n_.js","/_next/static/immutable/chunks/33bfn03fo8xnt.js","/_next/static/immutable/chunks/0tfjf78scu61b.js","/_next/static/immutable/chunks/31xnvej4n1bi9.js"],"default"] 1a:T6a7,

Multiple ramets of five genets of Acropora hemprichii and five genets of Porites lobata were collected from two sites (protected and exposed) used in two experiments: an 18 h acute thermal stress assay using the Coral Bleaching Automated Stress System (CBASS) and a two-week prolonged heating experiment using the indoor aquarium system at the King Abdullah University of Science and Technology (KAUST) Coastal and Marine Resources laboratory. Physiological responses of the coral host and associated endosymbionts were measured during (Fv/Fm, and phosynthesis and respiration) and at the end of the experiments (Final: symbiont densities, host protein, and chlorophyll a concentrations per cell and per cm2). Experimental tanks were ramped up from the 32°C control treatment to temperature treatments reaching 35°C, 36.5°C, and 38°C over 3h in the CBASS, and temperature treatments reaching 33.5°C, 35°C, and 36.5°C in the prolonged experiment at rates of 0.5 and 1.5°C per day. Each temperature treatment contained two replicate tanks (A and B).

Corals were subjected to short-term (7h) acute thermal profiles with four peak target temperatures (32°C, 35°C, 36.5°C, and 38°C), versus more prolonged heat exposures lasting 7 to 15 days, where temperatures were raised 0.5 and 1.5°C per day to four target temperatures (32°C, 33.5°C, 35°C, and 36.5°C). Physiological response metrics were recorded during and at the end of the experiments.

This dataset includes measurements of Fv/Fm from both experiments.

Problem report:
Missing data were samples that were not measured or that are missing due to sample mortality.

1b:T46a,Plate readers (also known as microplate readers) are laboratory instruments designed to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. Sample reactions can be assayed in 6-1536 well format microtiter plates. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 uL per well. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µL per well. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization. From: http://en.wikipedia.org/wiki/Plate_reader, 2014-09-0-23.1c:T13c4,NSF Award Abstract: The past few years have seen an unprecedented amount of coral bleaching across the globe. Global bleaching events in 2015-17, severely impacting iconic coral reefs in places such as the Great Barrier Reef, Micronesia, Hawaiian Islands, and Caribbean, were the worst recorded in recent human history. When ocean temperatures rise, the symbiosis between reef-building corals and their photosynthetic algae deteriorates, many times resulting in widespread coral die-offs as corals can starve without their symbiotic partners to supply food. These widespread events can have drastic impacts on ocean health and biodiversity, as well as the communities that depend on reefs for fishing, tourism, and protection from storms. Importantly, some corals resist or recover from bleaching better than others. Such variability in coral response to ocean warming could be critical to reef survival in the future, yet the scientific community lacks any standardized diagnostics to rapidly assess bleaching tolerance limits. Here, we plan to: 1) develop a standardized, short-term exposure to assess bleaching limits (analogous to cardiac stress tests for humans), 2) design an experimental system capable of delivering a range of thermal treatments as an open-source, low-cost, highly-portable device that can be readily adapted for bleaching tests in a wide variety of coral habitats, and 3) disseminate the results, instructions, and technologies to the reef research and conservation community through a combination of hands-on workshops, online outreach materials, press releases, and open-access research publications. Widespread dissemination of project products will be achieved via hands-on demonstrations and workshops in key geographic areas (Middle East, Caribbean, and Indo-Pacific), with a focus on the assembly of the system and operation of the experimental assay using local corals. This project will train both graduate students and a postdoctoral researcher, and brings together a team of national and global researchers in a collaborative investigation to address the international problem of coral bleaching. With each passing year, coral bleaching has shifted from an issue of serious sporadic concern to a critical widespread threat to reefs across the globe that is increasing in frequency and severity. However, during widespread bleaching events, some scattered corals and reef sections are able to survive better than others. Whether this is due to acclimatization or adaptation in thermal stress tolerance, this variability in response is critical to coral resilience to climate impacts. Currently, the scientific community lacks a standardized approach to rapidly assess coral thermal limits and identify resilient individuals or populations. Present day approaches range from observational surveys of natural bleaching and mortality, to multiple weeks of controlled chronic thermal exposure, to rapid, single or multi-day acute heat shocks. To what degree bleaching response varies across short-term versus longer-term experiments and how these responses compare to natural bleaching patterns is largely unknown. Using a group of coral species representative of a historical range of bleaching susceptibility (e.g., Acropora hemprichii, Pocillopora meandrina, and Porites lobata), research will address this important knowledge gap by experimental evaluation of the bleaching response to acute (0 - 2 day) versus chronic (>4 week) thermal stress. The overarching questions for this study are: how are the acute and chronic coral bleaching responses related, and can investigators predict ecologically relevant bleaching outcomes from the response to a short-term, acute heat-stress? To answer these questions, the research team will: 1) objectively compare acute versus chronic heat-stress exposures and synthesize a variety of response metrics based on core physiological measurements to develop a standardized, short-term thermal assay and diagnostic approach to rapidly assess bleaching, 2) operationalize an experimental system built around an open-source, cost-effective, easily transportable temperature control technology, and 3) distribute the results, experimental procedures, and temperature controlling technologies to the reef research and conservation communities. This project will produce an affordable experimental system and short-term diagnostic capable of determining coral thermal limits in just a few days in almost any location with reliable access to seawater and electricity or a portable generator. The research fills a critical knowledge gap through the development of a standardized set of diagnostic tools to assess coral thermal vulnerability before widespread bleaching events actually occur, so that proactive conservation and management strategies can be implemented ahead of widespread impacts to reef ecosystems. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.6:["$","$L19",null,{"dataset":{"_id":"858112","description":"","problem":"","acquisition":"$1a","processing":"

Data Processing:
\nData were organised using R statistical software (version 4.0.3).

BCO-DMO Processing:
\n- renamed fields to comply with BCO-DMO naming conventions;
\n- replaced 'NA' with 'nd' (no data);
\n- changed date format to YYYY-MM-DD.

The originally submitted GitHub repository https://github.com/BarshisLab/Heating-Duration-vs-Intensity-vs-RampRate was forked to https://github.com/BCODMO/Heating-Duration-vs-Intensity-vs-RampRate and tagged with release 1.0, which corresponds with this dataset submission. The original repository may have continued updates.

\n","bcodmoProcessing":"","title":"Maximum potential quantum efficiency of Photosystem II (Fv/Fm) for two coral species, Acropora hemprichii and Porites lobata, in response to thermal stress","nickname":"Fv/Fm","dataUrl":"","currentState":"Final no updates expected","currentStateId":"7","validated":true,"restricted":false,"version":"1","version_date":"2021-08-10T04:00:00.000Z","abstract":"This dataset contains values of Fv/Fm (maximum potential quantum efficiency of Photosystem II) for two coral species, Acropora hemprichii and Porites lobata, in response to thermal stress. Experiments were performed in Thuwal, Saudi Arabia in July-August 2019.","citation":"Evensen, N. R., Warner, M. E., Barshis, D. J. (2021) Maximum potential quantum efficiency of Photosystem II (Fv/Fm) for two coral species, Acropora hemprichii and Porites lobata, in response to thermal stress. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-08-10 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.858112.1 [access date]","citationParts":{"identifier":"doi:10.26008/1912/bco-dmo.858112.1","versionDate":"2021-08-10","versionId":"1","year":"2021","title":"Maximum potential quantum efficiency of Photosystem II (Fv/Fm) for two coral species, Acropora hemprichii and Porites lobata, in response to thermal stress","authors":"Evensen, N. R., Warner, M. E., Barshis, D. J.","publisher":"Biological and Chemical Oceanography Data Management Office (BCO-DMO)."},"doi":"10.26008/1912/bco-dmo.858112.1","release_date":null,"field_dataset_location":"","temporal_extent":{"start":"2019-07-29T04:00:00.000Z","end":"2019-08-11T04:00:00.000Z"},"temporal":{"resolution":"time:unitDay","type":"time:Interval","start":{"value":"1564372800","label":"2019-07-29"},"end":{"value":"1565496000","label":"2019-08-11"}},"spatial_extent":{"shape":"point","center":{"latitude":22.275795,"longitude":39.113112},"wkt":" POINT (39.113112 22.275795)","north":0,"east":0,"south":0,"west":0},"dataset_type":["experimental"],"keywords":["oceans"],"data_files":[{"id":"903447","title":"Fv_Fm.csv","url":"https://datadocs.bco-dmo.org/dataset/858112/file/XYYPvVouXq5Mq3/Fv_Fm.csv","showTableUrl":"https://www.bco-dmo.org/dataset/858112/data/view/903447","description":"Primary data file for dataset ID 858112","filename":"Fv_Fm.csv","bytesize":237616,"checksum":{"value":"8f09c4461bf2a1ec6c38905980598f45","Algorithm":{"_id":"190","name":"MD5"}},"mimetype":"text/csv","fileType":{"_id":"192","name":"Comma Separated Values (.csv)"},"isErddap":"","date":"","isPublicDuringEmbargo":false,"primaryDataFile":true,"showTable":true,"restricted":false}],"supplemental_files":[{"id":"858320","title":"Heating-Duration-vs-Intensity-vs-RampRate-1.0.zip","url":"https://datadocs.bco-dmo.org/docs/305/EAGER-CBASS/data_docs/Heating-Duration-vs-Intensity-vs-RampRate-1.0.zip","showTableUrl":"","description":"Code used to analyze and plot data; associated with BCO-DMO datasets 858112, 858150, and 858180.\r\n\r\n\r\nThese files are also available in the following GitHub repository: https://github.com/BCODMO/Heating-Duration-vs-Intensity-vs-RampRate/releases/tag/1.0","filename":"Heating-Duration-vs-Intensity-vs-RampRate-1.0.zip","bytesize":10375058,"checksum":{"value":"a762f0b8e1419f74653bf7da72a16296","Algorithm":{"_id":"190","name":"MD5"}},"mimetype":"application/zip","fileType":{"_id":"196","name":"ZIP Archive (ZIP)"},"isErddap":"","date":"","isPublicDuringEmbargo":false,"primaryDataFile":false,"showTable":false,"restricted":false}],"roles":[{"role":{"_id":"138","name":"Principal Investigator"},"performedBy":{"_id":"776332","name":"Daniel J. Barshis"},"performedAtOrganization":{"_id":"49","name":"Old Dominion University"}},{"role":{"_id":"140","name":"Co-Principal Investigator"},"performedBy":{"_id":"51387","name":"Mark E. Warner"},"performedAtOrganization":{"_id":"70","name":"University of Delaware"}},{"role":{"_id":"130","name":"Scientist"},"performedBy":{"_id":"640104","name":"Nicolas R. Evensen"},"performedAtOrganization":{"_id":"49","name":"Old Dominion University"}},{"role":{"_id":"145","name":"BCO-DMO Data Manager"},"performedBy":{"_id":"51498","name":"Shannon Rauch"},"performedAtOrganization":{"_id":"191","name":"Woods Hole Oceanographic Institution"}}],"deployments":[],"funding":[{"_id":"776326","name":"OCE-1833215","fundingSource":{"_id":"355","name":"NSF Division of Ocean Sciences"},"url":"https://www.nsf.gov/awardsearch/show-award?AWD_ID=1833215"},{"_id":"776330","name":"OCE-1833201","fundingSource":{"_id":"355","name":"NSF Division of Ocean Sciences"},"url":"https://www.nsf.gov/awardsearch/show-award?AWD_ID=1833201"}],"instruments":[{"_id":"858131","name":"3D scanner (HDI 120, LMI Technologies Inc.)","description":"","type_id":"752673","type_name":"3D scanner","type_desc":"A 3D scan captures digital information about the shape of an object with equipment that uses a laser or light to measure the distance between the scanner and the object.","type_acronym":""},{"_id":"858121","name":"","description":"","type_id":"711","type_name":"Aquarium","type_desc":"Aquarium - a vivarium consisting of at least one transparent side in which water-dwelling plants or animals are kept","type_acronym":"Aquarium"},{"_id":"920797","name":"CBASS","description":"","type_id":"920786","type_name":"Coral Bleaching Automated Stress System","type_desc":"CBASS, which stands for \"Coral Bleaching Automated Stress System\", are portable, field-deployable experimental tanks used to apply rapid, acute heat stress challenges. This system is described in:\r\n\r\nVoolstra, C. R., Buitrago‐López, C., Perna, G., Cárdenas, A., Hume, B. C. C., Rädecker, N., & Barshis, D. J. (2020). Standardized short‐term acute heat stress assays resolve historical differences in coral thermotolerance across microhabitat reef sites. Global Change Biology, 26(8), 4328-4343. Portico. https://doi.org/10.1111/gcb.15148","type_acronym":"CBASS"},{"_id":"858128","name":"flow cytometer (BD LSRFortessa, BD Biosciences)","description":"","type_id":"660","type_name":"Flow Cytometer","type_desc":"Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells. Description from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm","type_acronym":"Flow Cytometer"},{"_id":"858130","name":"Pulse amplitude-modulated (PAM) fluorometer (MINI-PAM, WALZ)","description":"","type_id":"484","type_name":"Fluorometer","type_desc":"A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.","type_acronym":"Fluorometer"},{"_id":"858125","name":"handheld homogenizer (Diax 900, Heidolph Instruments)","description":"","type_id":"522984","type_name":"Homogenizer","type_desc":"A homogenizer is a piece of laboratory equipment used for the homogenization of various types of material, such as tissue, plant, food, soil, and many others.","type_acronym":"Homogenizer "},{"_id":"858124","name":"fiberoptic oxygen probes (FireSting O2, Pyroscience)","description":"","type_id":"705","type_name":"Oxygen Sensor","type_desc":"An electronic device that measures the proportion of oxygen (O2) in the gas or liquid being analyzed","type_acronym":"Dissolved Oxygen Sensor"},{"_id":"858127","name":"SpectraMax Paradigm Multi‐Mode Microplate Reader (Molecular Devices)","description":"","type_id":"528693","type_name":"plate reader","type_desc":"$1b","type_acronym":""}],"parameters":[{"_id":"858133","name":"Experiment","format":"","frictionlessType":"string","description":"

experiment: CBASS = Coral Bleaching Automated Stress System; Prolonged = two-week prolonged heating experiment at the King Abdullah University of Science and Technology (KAUST)

","units":{"_id":"","name":"unitless"},"type":{"_id":"1850","name":"exp_type","description":"

Experiment type or description

"}},{"_id":"858134","name":"Species","format":"","frictionlessType":"string","description":"

species: Acropora or Porites

","units":{"_id":"","name":"unitless"},"type":{"_id":"976","name":"species","description":"

a taxonomic binomial that consists of a genus name followed by the species name of an organism

"}},{"_id":"858135","name":"Date","format":"%Y-%m-%d","frictionlessType":"date","description":"

date (local time zone: UTC/GMT+3:00); format: YYYY-MM-DD

","units":{"_id":"","name":"unitless"},"type":{"_id":"1070","name":"date","description":"

date; generally reported in GMT as YYYYMMDD (year; month; day); also as MMDD (month; day); EqPac dates are local Hawaii time. ISO_Date format is YYYY-MM-DD (http://www.iso.org/iso/home/standards/iso8601.htm)

"}},{"_id":"858136","name":"Experiment_Day","format":"","frictionlessType":"integer","description":"

experiment day

","units":{"_id":"","name":"unitless"},"type":{"_id":"472026","name":"time_point","description":"

Arbitrary time point of sampling in a sampling sequence (T0, T1,....Tn)

"}},{"_id":"858137","name":"Treatment_temperature","format":"","frictionlessType":"number","description":"

treatment temperature

","units":{"_id":"","name":"degrees Celsius"},"type":{"_id":"489066","name":"treatment","description":"

Experimental conditions applied to experimental units. In comparative experiments, members of the complementary group, the control group, receive either no treatment or a standard treatment.

"}},{"_id":"858138","name":"Ramp","format":"","frictionlessType":"string","description":"

method of ramping up temperature

","units":{"_id":"","name":"unitless"},"type":{"_id":"489066","name":"treatment","description":"

Experimental conditions applied to experimental units. In comparative experiments, members of the complementary group, the control group, receive either no treatment or a standard treatment.

"}},{"_id":"858139","name":"Tank","format":"","frictionlessType":"string","description":"

tank ID

","units":{"_id":"","name":"unitless"},"type":{"_id":"1992","name":"tank","description":"

Tank Number or Tank Id. Used to identify tanks/containers used in various experiments

"}},{"_id":"858140","name":"Site","format":"","frictionlessType":"string","description":"

description of coral collection site: exposed or protected

","units":{"_id":"","name":"unitless"},"type":{"_id":"1740","name":"site_descrip","description":"

Description or characteristics of a sampling site

"}},{"_id":"858141","name":"Genotype","format":"","frictionlessType":"integer","description":"

genotype

","units":{"_id":"","name":"unitless"},"type":{"_id":"976","name":"species","description":"

a taxonomic binomial that consists of a genus name followed by the species name of an organism

"}},{"_id":"858142","name":"Replicate","format":"","frictionlessType":"string","description":"

replicate: each temperature treatment contained two replicate tanks (A and B)

","units":{"_id":"","name":"unitless"},"type":{"_id":"953","name":"replicate","description":"

an identifier used to distinguish between one or more replicate samples

"}},{"_id":"858143","name":"Fv_Fm","format":"","frictionlessType":"number","description":"

measurements of dark adapted maximum quantum yield of photosystem II of the algal symbionts (Fv/Fm) for each ramet (a unitless ratio)

","units":{"_id":"","name":"unitless"},"type":{"_id":"642868","name":"Fv_Fm","description":"

The difference between maximum fluorescence and minimum fluorescence is Fv, or variable fluorescence. Fv/Fm is a normalized ratio created by dividing variable fluorescence by maximum fluorescence.

"}},{"_id":"858144","name":"Heatload32","format":"","frictionlessType":"number","description":"

the number of heatloading hours (prolonged experiment) or minutes (CBASS experiment) above 32°C experienced by the corals at the time of the Fv/Fm measurement. Heatloading is a factor of time and degrees above the temperature threshold (i.e., 1 hour at 2°C above the temperature threshold is equal to 2 heatloading hours)

","units":{"_id":"","name":"heatloading hours (prolonged) or minutes (CBASS) above 32°C"},"type":{"_id":"555754","name":"duration","description":"

Duration of treatment, observation or sampling

"}},{"_id":"858145","name":"Heatload33","format":"","frictionlessType":"number","description":"

the number of heatloading hours (prolonged experiment) or minutes (CBASS experiment) above 33°C experienced by the corals at the time of the Fv/Fm measurement. Heatloading is a factor of time and degrees above the temperature threshold (i.e., 1 hour at 2°C above the temperature threshold is equal to 2 heatloading hours)

","units":{"_id":"","name":"heatloading hours (prolonged) or minutes (CBASS) above 33°C"},"type":{"_id":"555754","name":"duration","description":"

Duration of treatment, observation or sampling

"}},{"_id":"858146","name":"Heatload34","format":"","frictionlessType":"number","description":"

the number of heatloading hours (prolonged experiment) or minutes (CBASS experiment) above 34°C experienced by the corals at the time of the Fv/Fm measurement. Heatloading is a factor of time and degrees above the temperature threshold (i.e., 1 hour at 2°C above the temperature threshold is equal to 2 heatloading hours)

","units":{"_id":"","name":"heatloading hours (prolonged) or minutes (CBASS) above 34°C"},"type":{"_id":"555754","name":"duration","description":"

Duration of treatment, observation or sampling

"}},{"_id":"858147","name":"Temperature","format":"","frictionlessType":"number","description":"

temperature

","units":{"_id":"","name":"degrees Celsius"},"type":{"_id":"555754","name":"duration","description":"

Duration of treatment, observation or sampling

"}},{"_id":"858148","name":"ElapsedTime","format":"","frictionlessType":"integer","description":"

hours elapsed

","units":{"_id":"","name":"hours"},"type":{"_id":"1838","name":"time_elapsed","description":"

Elapsed time. Units can be seconds, minutes, etc.

"}}],"related_publications":[],"related_datasets":[{"_id":"858180","name":"Photosynthesis and Respiration","url":"","relation_type":"IsRelatedTo","type_desc":"

The dataset is related to another dataset.

\n","relation_desc":"These datasets resulted from the same set of experiments.","citation":"Evensen, N. R., Warner, M. E., Barshis, D. J. (2021) Photosynthesis and respiration data recorded during experiments conducted to examine two coral species' responses to thermal stress. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-08-11 doi:10.26008/1912/bco-dmo.858180.1"},{"_id":"858150","name":"Final Physiology","url":"","relation_type":"IsRelatedTo","type_desc":"

The dataset is related to another dataset.

\n","relation_desc":"These datasets resulted from the same set of experiments.","citation":"Evensen, N. R., Warner, M. E., Barshis, D. J. (2021) Physiological metrics recorded at the end of experiments conducted to examine two coral species' responses to thermal stress. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-08-11 doi:10.26008/1912/bco-dmo.858150.1"}],"services":[{"grouping":"","items":[{"name":"View Data","description":"An in-browser view of the primary tabular data file","url":"https://www.bco-dmo.org/dataset/858112/data/view","contentType":"text/html","actionType":"view"}]},{"grouping":"Metadata","items":[{"name":"HTML","description":"A simple HTML page of the dataset metadata","url":"https://www.bco-dmo.org/dataset/858112/description","contentType":"text/html","actionType":"view"},{"name":"PDF","description":"A PDF of the HTML dataset metadata","url":"https://www.bco-dmo.org/dataset/858112/Dataset_description.pdf","contentType":"application/pdf","actionType":"download"},{"name":"ISO 19115-2 (NOAA Profile)","description":"","url":"https://www.bco-dmo.org/dataset/858112/iso","contentType":"application/xml","actionType":"download"}]}],"programs":[],"projects":[{"_id":"776327","name":"EAGER: Collaborative Research: Bleaching phenotypes of acute vs. chronic coral bleaching susceptibility and resilience: towards a standardized coral resilience diagnostic","acronym":"EAGER-CBASS","description":"$1c"}],"archives":[{"id_value":"10.26008/1912/bco-dmo.858112.1","url":"https://doi.org/10.26008/1912/bco-dmo.858112.1","date_assigned":"2021-08-11","version_date":"2021-08-10","archiver":"Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)","archiver_id":"272"}],"license":{"name":"Creative Commons Attribution 4.0","id":"CC-BY-4.0","uri":"https://creativecommons.org/licenses/by/4.0/","spdxUri":"https://spdx.org/licenses/CC-BY-4.0"},"metrics":{"views":{"count":210},"downloads":{"count":454}}}}]