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&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Known Problems/Issues:&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
There is some difference in the fluorometer data between the up and down casts. We are not sure what is causing this just yet. This can be seen as an obvious striping pattern in the profile plots. The oxygen sensor has a time lag of 5-7 seconds. Applying this amount of lag helps align the up and down casts well. The data here are not time shifted.&amp;lt;/p&amp;gt;</gco:CharacterString>
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With climate change, ocean temperatures are expected to increase which in turn will reduce oxygen availability and increase metabolic oxygen demand in marine organisms. The investigators will conduct shipboard physiological experiments for various marine organisms and determine their distributions in relation to environmental conditions within an oxygen minimum zone (OMZ) in the Eastern Pacific Ocean. The goal will be to model and map a Metabolic Index (MI) to predict how vertical and horizontal distributions for these species might change throughout the world's oceans in the future. The MI is defined as the ratio between environmental oxygen supply and temperature-dependent oxygen demand. Oxygen supply includes both the environmental oxygen concentration across a habitat range and the physiological features of organisms that facilitate oxygen uptake, such as gills and circulatory systems. Thus, the MI will integrate measured tolerance and environmental exposure to low oxygen with environmental data. The investigators will measure tolerance to low oxygen, focusing on under-studied organisms, including the effect of temperature and organism size. They will sample along a natural gradient in oxygen content south of the California Current in the Eastern Pacific. The science team and a videographer will develop a blog about deep-sea biology and climate change using web-based and video technologies. Four graduate students will be funded on this project, and in conjunction with a recently developed course in pelagic ecology, several undergraduates will have the opportunity to participate in seagoing research.&lt;/p&gt;
&lt;p&gt;This research fills a critical need for a physiology-based metric that can be used to predict changing marine communities as the oceans warm and hypoxic zones expand. Modern OMZs are extensive and characterized by deep-water (300-800 m) oxygen partial pressures lethal to most marine organisms, yet thriving communities exist there. Climate change is predicted to further deplete oxygen. The investigators will model and map a Metabolic Index (MI) for diverse marine species to help predict how in vertical and horizontal distributions of species may change throughout the world's oceans in the future. The MI will derive oxygen supply and demand data from published and planned measurements of the minimum environmental partial pressure of oxygen to which individual species are exposed (based on their distributions in the water column) and the minimum requirements to support routine aerobic metabolic demand (from shipboard respiration measurements of individuals). During research cruises in the Eastern Pacific along a gradient of OMZ intensity, the investigators will conduct shipboard physiological measurements to determine metabolic demand for understudied mesozooplankton and gelatinous taxa and determine the size- and temperature dependence for diverse species for incorporation into the MI. Vertically-stratified net sampling and in situ photography will identify and characterize unique OMZ community features, such as the lower oxycline biomass peak present in some OMZs and the oxygen-dependence of day and night habitat depths for vertically-migrating species. The MI will be mapped using climatological data to both test and generate hypotheses about the response of oceanic communities to climate change. In preliminary analysis, the MI suggests a metabolic constraint at a MI of ~2 that may act to limit vertical and horizontal habitat ranges.&lt;/p&gt;</gco:CharacterString>
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	Name: deployment_id
	Units: unitless
	Description: &lt;p&gt;identifier for the deployment; indicates the start date and time of deployment in format: YYYYMMDD_hhmmss (time zone is GMT)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/860321.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/860322.rdf
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	Description: &lt;p&gt;date-time string converted to ISO8601 format: YYYY-MM-DDThh:mm:ss.xxxxxxZ. Note that data are accurate to milliseconds (not microseconds)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/860323.rdf
	Name: Julian_day
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http://lod.bco-dmo.org/id/dataset-parameter/860324.rdf
	Name: Unix_time_sec
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	Description: &lt;p&gt;Unix time&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/860325.rdf
	Name: Lat
	Units: degrees North
	Description: &lt;p&gt;latitude of the Wire Flyer&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/860326.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/860327.rdf
	Name: Depth_m
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http://lod.bco-dmo.org/id/dataset-parameter/860328.rdf
	Name: Vertical_velocity_m_per_sec_pos_down
	Units: meters per second (m/s)
	Description: &lt;p&gt;vertical velocity&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/860329.rdf
	Name: Temperature_C
	Units: degrees Celsius
	Description: &lt;p&gt;temperature&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/860330.rdf
	Name: Pot_temperature_C
	Units: degrees Celsius
	Description: &lt;p&gt;potential temperature calculated using seawater toolbox&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/860331.rdf
	Name: Pot_density
	Units: kilograms per cubic meter (kg/m^3)
	Description: &lt;p&gt;potential density calculated using seawater toolbox&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/860332.rdf
	Name: Pressure_db
	Units: decibars (db)
	Description: &lt;p&gt;pressure&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/860333.rdf
	Name: Conductivity_sm
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	Description: &lt;p&gt;conductivity&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/860334.rdf
	Name: Salinity_PSU
	Units: PSU
	Description: &lt;p&gt;salinity calculated using seawater toolbox&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/860335.rdf
	Name: Sound_velocity_m_per_sec
	Units: meters per second (m/s)
	Description: &lt;p&gt;sound velocity calculated using seawater toolbox&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/860336.rdf
	Name: O2_con_umol
	Units: micromoles (umol)
	Description: &lt;p&gt;oxygen concentration&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/860337.rdf
	Name: optode_temp_C
	Units: degrees Celsius
	Description: &lt;p&gt;optode temperature&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/860338.rdf
	Name: Air_sat_percent
	Units: unitless (percent)
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http://lod.bco-dmo.org/id/dataset-parameter/860340.rdf
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	Units: micrograms per liter (ug/L)
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http://lod.bco-dmo.org/id/dataset-parameter/860341.rdf
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&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Known Problems/Issues:&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
There is some difference in the fluorometer data between the up and down casts. We are not sure what is causing this just yet. This can be seen as an obvious striping pattern in the profile plots. The oxygen sensor has a time lag of 5-7 seconds. Applying this amount of lag helps align the up and down casts well. The data here are not time shifted.&amp;lt;/p&amp;gt;</gco:CharacterString>
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&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;BCO-DMO Processing:&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
- concatenated data from 12 separate files into one dataset;&amp;lt;br /&amp;gt;
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            <gco:CharacterString>PI Supplied Instrument Name: Wet Labs Flbb-2k PI Supplied Instrument Description:The Chl and turbidity are from a Wet Labs Flbb-2k, 470/695 nm Chl-a and 700nm turbidity. Instrument Name: WETLabs ECO FLBB scattering fluorescence sensor Instrument Short Name:WETLabs ECO FLBB   Instrument Description: A dual-optical-sensor that carries a single-wavelength chlorophyll fluorometer (470nm ex/695nm em) and backscattering sensor (700 nm) that measures phytoplankton and particle concentration. It operates by using blue (470nm) and red (700 nm) LEDs that alternately flash. The blue LED stimulates chlorophyll fluorescence in plants while the red light illuminates the total particle field. The backscattering sensor has an in-water centroid angle of 142 degrees and can be calibrated to measure turbidity. The fluorometer can typically measure phytoplankton concentrations in the range 0-30 ug/l, with a sensitivity of 0.015 ug/l. The backscattering sensor can measure within the range 0-3 m-1, with a sensitivity of 0.0015 m-1. The instrument output in the standard version is digital and uses a low power mode and stores data. Other variants are used. The instrument is rated to a depth of 600m as standard, with the options of deeper instruments rated up to 6000m and instruments with bio-wipers, rated to 300 m.

This instrument comes in the following optional models: FLbb(RT), FLbb(RT)D, FLbbB, FLbbS, FLbbBS, FLbb2k. Refer to the datasheet from the manufacturer: https://www.seabird.com/asset-get.download.jsa?id=55460873804</gco:CharacterString>
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The Wire Flyer towed vehicle is a platform able to collect high-resolution water column sections. The vehicle is motivated by a desire to effectively capture spatial structures at the submesoscale. The Wire Flyer profiles up and down along a ship-towed cable autonomously using controllable wings for propulsion. At ship speeds between 2 and 5 kt (1.02–2.55 m s−1), the vehicle is able to profile over prescribed depth bands down to 1000 m. The vehicle carries sensors for conductivity, temperature, depth, oxygen, turbidity, chlorophyll, pH, and oxidation reduction potential. During normal operations, the vehicle is typically commanded to cover vertical regions between 300 and 400 m in height with profiles that repeat at kilometer spacing. The vertical profiling speed can be user-specified up to 150 m min−1. During operations, an acoustic modem is used to communicate with the vehicle to provide status information, data samples, and the ability to modify the sampling pattern.  

Detailed information can be found in the following publication:
Roman, C., Ullman, D. S., Hebert, D., &amp; Licht, S. (2019). The Wire Flyer Towed Profiling System. Journal of Atmospheric and Oceanic Technology, 36(2), 161–182. doi:10.1175/jtech-d-17-0180.1</gco:CharacterString>
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