http://lod.bco-dmo.org/id/dataset/862346
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2021-10-04
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Physiological response of eight Palau coral colonies to thermal stress as seen in temperature experiments in 2014 and 2015
2021-10-04
publication
2021-10-04
revision
BCO-DMO Linked Data URI
2021-10-04
creation
http://lod.bco-dmo.org/id/dataset/862346
Todd Christopher LaJeunesse
Pennsylvania State University
principalInvestigator
Dustin Kemp
University of Alabama at Birmingham
principalInvestigator
Mark E. Warner
University of Delaware
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Hoadley, K., LaJeunesse, T. C., Warner, M. E., Kemp, D. (2021) Physiological response of eight Palau coral colonies to thermal stress as seen in temperature experiments in 2014 and 2015. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-10-04 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/862346 [access date]
Palau coral physiology and temperature Dataset Description: Methods and Sampling: <p><strong>Sampling and analytical procedures: </strong></p>
<p><strong><em>Coral Collection:</em></strong> A total of 8 colonies of each species were collected at each site at a depth between 5–10 meters (offshore) or 1–5 meters (Inshore) and at least 10 meters apart. Differences in collection depth were necessary due to the natural distribution of these species at each site and in order to ensure all colonies were collected from similar light conditions (maximal mid-day <em>in situ </em>light of 800-1000-μmol quanta m<sup>-2</sup> s<sup>-1</sup>). Sampling was performed offshore at Rebotel Reef (7.248833, 134.235817) and inshore at Nikko Bay (7.3248, 134.4934).&nbsp;Colonies were transported back to the Palau International Coral Research Center (PICRC) and fragmented into five replicate nubbins and placed into a 1200L flow-through aquarium and held at 27.5°C. Seawater was collected directly off of a nearby pier at a depth of 3 m and then passed through a pressurized sand filter and aquarium filter pads prior to use in flow-through and experimental treatment systems. Coral nubbins were attached to 2-inch square PVC tiles with marine epoxy (Splash zone compound A-788) and held at ambient conditions in flow-through bins as described above for 12-16 days prior to the start of the experiment. Control and experimental bins (see below) were maintained outdoors underneath clear plastic film (Sun Selector, Ginegar Plastic Products) to block periodic rainfall and a 60% shade cloth providing a peak midday light intensity of 800 μmol quanta m<sup>-2</sup> s<sup>-1</sup>, as measured with a light sensor (LiCor LI-192).</p>
<p><strong><em>Experimental System:</em></strong> Each treatment system consisted of 7–12 (56 L) plastic treatment bins connected to a central (~1200 L) sump. Seawater within each sump was set to either heated or ambient temperature conditions prior to being sent to the treatment bins. Ambient temperature was maintained via a chiller system and a series of titanium heating elements were used for high temperature treatments. For each treatment, three replicate fragments from each colony were placed within separate treatment bins. For the heated treatment, the temperature was gradually ramped from 27.5°C to 32°C over 4 days, and then maintained at 32°C for an additional 10 days for a total of 14 days of heating. Temperature within the control treatment was maintained at 27.5°C throughout the 14-day experiment. Treatment bins and PVC tiles were cleaned every other day to prevent algal fouling, and coral fragments were rotated within their respective bins every other day to ensure a uniform light exposure and minimize possible tank effects.</p>
<p>At the start of the experiment (day 0), one fragment from each coral colony was removed from control and treatment tanks and processed for symbiont photo-physiology and biomass metrics (described below). Additional fragments were then sampled on days 9 (4 days of temperature ramping + 5 days at 32°C) and 14 (4 days of temperature ramping and 10 days at 32°C). Coral tissue was removed by airbrush (100 psi) with filtered (0.22 m) seawater. The resulting slurry was homogenized with a Tissue Tearor (BioSpec products, Inc), and then divided into 2 mL aliquots. One aliquot was preserved with 1% glutaraldehyde for cell enumeration and stored at 4°C. All other aliquots were centrifuged for 2 minutes (5,000×<em>g</em>) and the supernatant was discarded. Algal subsamples from each colony were suspended in DNA preservation buffer (Seutin et al. 1991) and stored at 4°C. The remaining algal samples were immediately frozen (-20°C) and shipped back to the United States and stored at -20°C until further analyses.</p>
<p>Remaining information on procedures can be found in Hoadley et al., 2019.</p>
Funding provided by NSF Division of Integrative Organismal Systems (NSF IOS) Award Number: IOS-1719675 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1719675
Funding provided by NSF Division of Integrative Organismal Systems (NSF IOS) Award Number: IOS-1258058 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1258058
Funding provided by NSF Division of Integrative Organismal Systems (NSF IOS) Award Number: IOS-1258065 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1258065
onGoing
Todd Christopher LaJeunesse
Pennsylvania State University
814-863-2038
208 Mueller Laboratory Department of Biology
University Park
PA
16802
USA
tcl3@psu.edu
pointOfContact
Dustin Kemp
University of Alabama at Birmingham
205-934-1426
1720 Second Ave South
Birmingham
AL
35294
US
dwk@uab.edu
pointOfContact
Mark E. Warner
University of Delaware
302-645-4365
School of Marine Science and Policy 700 Pilottown Rd.
Lewes
DE
19958
USA
mwarner@udel.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Year
Location
Reef_site
Latitude
Longitude
local
Treatment
Host
Symbiont
Experimental_day
Colony_Fragment
Colony_Name_Frag_Code
Tau_PSII
Tau_PQ
ETR
NPQ
FvFm
Connectivity_rho
Sigma
Lipid
Protein
Carbohydrate
Symb_cell_volume
Gross_Photo
Chlorophyll_a
Cell_Density
resource_name
airbrush
Flow-through aquarium
chiller system
EVOS digital fluorescent microscope
BioSpec Tissue Tearor
titanium heating elements
LiCor LI-192
Fibox 4 fiber optic oxygen system (PreSens)
FLUOstar Omega plate reader (BMG Labtech, Germany)
Fluorescence Induction and Relaxation (FIRe) fluorometer (Satlantic Inc., Halifax)
theme
None, User defined
year
site description
latitude
longitude
No BCO-DMO term
treatment
sample type
sample identification
rate constant
Electron Transport Rate (ETR)
Fv to Fm ratio
lipids in biota
protein concentration
carbohydrates
volume
photosynthetic rate
chlorophyll a
file_name
featureType
BCO-DMO Standard Parameters
Airbrush
Aquarium
Aquarium chiller
Fluorescence Microscope
Homogenizer
Immersion heater
LI-COR LI-192 PAR Sensor
Oxygen Sensor
plate reader
Satlantic Fluorescence Induction and Relaxation of Emission Spectrometer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: The physiology and ecology of widespread 'stress tolerant' coral endosymbionts: coral 'saviors' or opportunistic invaders?
https://osprey.bco-dmo.org/project/948053
Collaborative Research: The physiology and ecology of widespread 'stress tolerant' coral endosymbionts: coral 'saviors' or opportunistic invaders?
<p><em><strong>NSF Award Abstract</strong></em><br />
Ocean warming is affecting life on our planet in many ways. High temperature can disrupt the endosymbioses between dinoflagellate algae (Symbiodinium spp.) and reef-building corals (i.e. coral bleaching), thereby risking the global loss of a critical marine ecosystem. The physiological, ecological and evolutionary responses of coral-dinoflagellate symbioses to environmental stress brought on by global climate change are complex. The spread of certain types of symbiotic algae may increase the thermal stress tolerance among corals and help them persist in warmer oceans, but perhaps not without trade-offs to the health of the coral. The dinoflagellate tentatively named Symbiodinium trenchi has become increasingly more common in numerous corals throughout the Caribbean, but is often at low-abundance relative to other symbionts. While S. trenchi can increase in abundance during and after warming, it is often displaced by other symbionts following a return to normal conditions. Genetic evidence indicates that S. trenchi recently invaded and/or expanded in the Caribbean and has developed associations with many corals that seem to be poorly optimized, or mal-adapted, relative to the symbioses it maintains with corals in the Indo-Pacific. This project will investigate the symbiosis ecology and physiology of S. trenchi in corals from the Atlantic and Pacific Oceans. Bleaching experiments will examine the effects of increased temperature on transfer of carbon from the algae to the host coral (via stable isotopic tagging), as well as photosynthesis and growth among colonies harboring S. trenchi compared to colonies harboring other Symbiodinium spp. The potential for symbiont community shifts as well as altered long-term colony growth based on bleaching severity and recovery time will be investigated. A reciprocal transplant study will examine the competitive interaction and stability of symbionts among Pacific corals. These studies will test if the continued spread of S. trenchi will affect coral growth in the Caribbean and whether it might behave similarly in the Indo-Pacific if environmental conditions worsen. The results from this project have the potential to supply transformative information regarding how (or if) a widely distributed symbiotic algal species may influence the resilience of reef-building corals and their potential to survive projected increases in ocean warming due to climate change. In addition to training one postdoctoral scholar and several graduate students, this project will enhance scientific discovery and participation of underrepresented groups via several outreach efforts with the Palau National Aquarium, Palau International Coral Reef Center, and local schools. Educational units in marine symbioses and science will be developed with several local high school teachers and students, and unique research opportunities will be provided to students at the Palau Community College. Likewise, a new educational display addressing how global climate may impact coral reefs, and describing the current research to better understand the physiology of coral-algal symbioses, will be developed and presented at the University of Delaware open house "Coast Day." The display will be donated subsequently to the Palau Aquarium for future use. This award is co-funded by NSF's Office of International and Integrative Activities.</p>
Coral Endosymbionts
largerWorkCitation
project
eng; USA
biota
oceans
134.235817
134.822333
7.248833
7.541333
2014-01-01
2015-12-31
Rebotel Reef and Nikko Bay in Palau
0
BCO-DMO catalogue of parameters from Physiological response of eight Palau coral colonies to thermal stress as seen in temperature experiments in 2014 and 2015
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/958032.rdf
Name: Year
Units: year
Description: <p>Year of sampling</p>
http://lod.bco-dmo.org/id/dataset-parameter/958033.rdf
Name: Location
Units: unitless
Description: <p>Location of coral sampling, either offshore (Rebotel reef) or inshore (Nikko Bay)</p>
http://lod.bco-dmo.org/id/dataset-parameter/958034.rdf
Name: Reef_site
Units: unitless
Description: <p>Reef site that was sampled, either Rebotel Reef (offshore) or Nikko Bay (inshore)</p>
http://lod.bco-dmo.org/id/dataset-parameter/958035.rdf
Name: Latitude
Units: units
Description: <p>Latitude of reef site where sampling occurred</p>
http://lod.bco-dmo.org/id/dataset-parameter/958036.rdf
Name: Longitude
Units: units
Description: <p>Longitude of reef site where sampling occurred</p>
http://lod.bco-dmo.org/id/dataset-parameter/958037.rdf
Name: local
Units: unitless
Description: <p>???</p>
http://lod.bco-dmo.org/id/dataset-parameter/958038.rdf
Name: Treatment
Units: unitless
Description: <p>Type of treatment, either control (C), ambient, or hightemp</p>
http://lod.bco-dmo.org/id/dataset-parameter/958039.rdf
Name: Host
Units: unitless
Description: <p>???</p>
http://lod.bco-dmo.org/id/dataset-parameter/958040.rdf
Name: Symbiont
Units: unitless
Description: <p>???</p>
http://lod.bco-dmo.org/id/dataset-parameter/958041.rdf
Name: Experimental_day
Units: ???
Description: <p>???</p>
http://lod.bco-dmo.org/id/dataset-parameter/958042.rdf
Name: Colony_Fragment
Units: unitless
Description: <p>???</p>
http://lod.bco-dmo.org/id/dataset-parameter/958043.rdf
Name: Colony_Name_Frag_Code
Units: unitless
Description: <p>???</p>
http://lod.bco-dmo.org/id/dataset-parameter/958044.rdf
Name: Tau_PSII
Units: microseconds
Description: <p>Rate constant for reoxidation of the Qa site of the D1 protein within the PSII RC</p>
http://lod.bco-dmo.org/id/dataset-parameter/958045.rdf
Name: Tau_PQ
Units: microseconds
Description: <p>Rate constant for reoxidation of the plastoquinole pool.</p>
http://lod.bco-dmo.org/id/dataset-parameter/958046.rdf
Name: ETR
Units: moles of electrons transferred per mole of PSII reaction centers per hour (mol e- mol RCII^-1h^-1)
Description: <p>RCII-specific electron transport rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/958047.rdf
Name: NPQ
Units: relative units
Description: <p>Non-photochemical quenching</p>
http://lod.bco-dmo.org/id/dataset-parameter/958048.rdf
Name: FvFm
Units: relative units
Description: <p>Dark acclimated maximum quantum yield of PSII</p>
http://lod.bco-dmo.org/id/dataset-parameter/958049.rdf
Name: Connectivity_rho
Units: relative units
Description: <p>Connectivity between PSII reaction centers</p>
http://lod.bco-dmo.org/id/dataset-parameter/958050.rdf
Name: Sigma
Units: squared angstroms per quantum (Å2q^-1)
Description: <p>Dark acclimated effective absorption cross section of PSII</p>
http://lod.bco-dmo.org/id/dataset-parameter/958051.rdf
Name: Lipid
Units: micrograms per cell (?g cell^-1)
Description: <p>Lipid concentration per cell</p>
http://lod.bco-dmo.org/id/dataset-parameter/958052.rdf
Name: Protein
Units: micrograms per cell (?g cell^-1)
Description: <p>Protein conenctration per cell</p>
http://lod.bco-dmo.org/id/dataset-parameter/958053.rdf
Name: Carbohydrate
Units: micrograms per cell (?g cell^-1)
Description: <p>Carbohydrate concentration per cell</p>
http://lod.bco-dmo.org/id/dataset-parameter/958054.rdf
Name: Symb_cell_volume
Units: cubic micrometers (?m^-3)
Description: <p>Symbiont cellular volume</p>
http://lod.bco-dmo.org/id/dataset-parameter/958055.rdf
Name: Gross_Photo
Units: milligrams oxygen per liter per minute per cell (mg O2 L^-1 min^-1 cell^-1)
Description: <p>Gross algal photosynthesis</p>
http://lod.bco-dmo.org/id/dataset-parameter/958056.rdf
Name: Chlorophyll_a
Units: picrograms per cell (pg cell^-1)
Description: <p>Chlorophyll concentration per cell</p>
http://lod.bco-dmo.org/id/dataset-parameter/958057.rdf
Name: Cell_Density
Units: ???
Description: <p>???</p>
http://lod.bco-dmo.org/id/dataset-parameter/958058.rdf
Name: resource_name
Units: unitless
Description: <p>Filenames of original data files. ** Why don't filenames indicate 2014 and 2015? **</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://osprey.bco-dmo.org/dataset/862346/data/download
download
onLine
dataset
<p><strong>Sampling and analytical procedures: </strong></p>
<p><strong><em>Coral Collection:</em></strong> A total of 8 colonies of each species were collected at each site at a depth between 5–10 meters (offshore) or 1–5 meters (Inshore) and at least 10 meters apart. Differences in collection depth were necessary due to the natural distribution of these species at each site and in order to ensure all colonies were collected from similar light conditions (maximal mid-day <em>in situ </em>light of 800-1000-μmol quanta m<sup>-2</sup> s<sup>-1</sup>). Sampling was performed offshore at Rebotel Reef (7.248833, 134.235817) and inshore at Nikko Bay (7.3248, 134.4934).&nbsp;Colonies were transported back to the Palau International Coral Research Center (PICRC) and fragmented into five replicate nubbins and placed into a 1200L flow-through aquarium and held at 27.5°C. Seawater was collected directly off of a nearby pier at a depth of 3 m and then passed through a pressurized sand filter and aquarium filter pads prior to use in flow-through and experimental treatment systems. Coral nubbins were attached to 2-inch square PVC tiles with marine epoxy (Splash zone compound A-788) and held at ambient conditions in flow-through bins as described above for 12-16 days prior to the start of the experiment. Control and experimental bins (see below) were maintained outdoors underneath clear plastic film (Sun Selector, Ginegar Plastic Products) to block periodic rainfall and a 60% shade cloth providing a peak midday light intensity of 800 μmol quanta m<sup>-2</sup> s<sup>-1</sup>, as measured with a light sensor (LiCor LI-192).</p>
<p><strong><em>Experimental System:</em></strong> Each treatment system consisted of 7–12 (56 L) plastic treatment bins connected to a central (~1200 L) sump. Seawater within each sump was set to either heated or ambient temperature conditions prior to being sent to the treatment bins. Ambient temperature was maintained via a chiller system and a series of titanium heating elements were used for high temperature treatments. For each treatment, three replicate fragments from each colony were placed within separate treatment bins. For the heated treatment, the temperature was gradually ramped from 27.5°C to 32°C over 4 days, and then maintained at 32°C for an additional 10 days for a total of 14 days of heating. Temperature within the control treatment was maintained at 27.5°C throughout the 14-day experiment. Treatment bins and PVC tiles were cleaned every other day to prevent algal fouling, and coral fragments were rotated within their respective bins every other day to ensure a uniform light exposure and minimize possible tank effects.</p>
<p>At the start of the experiment (day 0), one fragment from each coral colony was removed from control and treatment tanks and processed for symbiont photo-physiology and biomass metrics (described below). Additional fragments were then sampled on days 9 (4 days of temperature ramping + 5 days at 32°C) and 14 (4 days of temperature ramping and 10 days at 32°C). Coral tissue was removed by airbrush (100 psi) with filtered (0.22 m) seawater. The resulting slurry was homogenized with a Tissue Tearor (BioSpec products, Inc), and then divided into 2 mL aliquots. One aliquot was preserved with 1% glutaraldehyde for cell enumeration and stored at 4°C. All other aliquots were centrifuged for 2 minutes (5,000×<em>g</em>) and the supernatant was discarded. Algal subsamples from each colony were suspended in DNA preservation buffer (Seutin et al. 1991) and stored at 4°C. The remaining algal samples were immediately frozen (-20°C) and shipped back to the United States and stored at -20°C until further analyses.</p>
<p>Remaining information on procedures can be found in Hoadley et al., 2019.</p>
Specified by the Principal Investigator(s)
- Loaded data from two source files
- Combined data from two source files into a single data table
- Added columns for Latitude and Longitude corresponding to coordinates for the offshore site (Rebotel Reef) and the inshore site (Nikko Bay)
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
airbrush
airbrush
PI Supplied Instrument Name: airbrush PI Supplied Instrument Description:Coral tissue was removed by airbrush (100 psi) with filtered (0.22 m) seawater. Instrument Name: Airbrush Instrument Short Name:Airbrush Instrument Description: Device for spraying liquid by means of compressed air.
Flow-through aquarium
Flow-through aquarium
PI Supplied Instrument Name: Flow-through aquarium PI Supplied Instrument Description:Coral colonies were fragmented into five replicate nubbins and placed into a 1200L flow-through aquarium and held at 27.5°C Instrument Name: Aquarium Instrument Short Name:Aquarium Instrument Description: Aquarium - a vivarium consisting of at least one transparent side in which water-dwelling plants or animals are kept
chiller system
chiller system
PI Supplied Instrument Name: chiller system PI Supplied Instrument Description:Ambient temperature was maintained via a chiller system Instrument Name: Aquarium chiller Instrument Short Name:Aquarium chiller Instrument Description: Immersible or in-line liquid cooling device, usually with temperature control.
EVOS digital fluorescent microscope
EVOS digital fluorescent microscope
PI Supplied Instrument Name: EVOS digital fluorescent microscope Instrument Name: Fluorescence Microscope Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB06/
BioSpec Tissue Tearor
BioSpec Tissue Tearor
PI Supplied Instrument Name: BioSpec Tissue Tearor PI Supplied Instrument Description:The resulting slurry was homogenized with a Tissue Tearor (BioSpec products, Inc) which is a rotor/stator type tissue homogenizer which rapidly homogenizes, disrupts, and emulsifies plant and animal samples in 0.5 - 1000 ml of liquid. Instrument Name: Homogenizer Instrument Short Name:Homogenizer Instrument Description: A homogenizer is a piece of laboratory equipment used for the homogenization of various types of material, such as tissue, plant, food, soil, and many others.
titanium heating elements
titanium heating elements
PI Supplied Instrument Name: titanium heating elements PI Supplied Instrument Description:a series of titanium heating elements were used for high temperature treatments Instrument Name: Immersion heater Instrument Short Name:Immersion heater Instrument Description: Submersible heating element for water tanks and aquaria.
LiCor LI-192
LiCor LI-192
PI Supplied Instrument Name: LiCor LI-192 PI Supplied Instrument Description:Light intensity measured with a light sensor (LiCor LI-192). Instrument Name: LI-COR LI-192 PAR Sensor Instrument Short Name:LI-COR LI-192 PAR Instrument Description: The LI-192 Underwater Quantum Sensor (UWQ) measures underwater or atmospheric Photon Flux Density (PPFD) (Photosynthetically Available Radiation from 360 degrees) using a Silicon Photodiode and glass filters encased in a waterproof housing. The LI-192 is cosine corrected and features corrosion resistant, rugged construction for use in freshwater or saltwater and pressures up to 800 psi (5500 kPa, 560 meters depth). Typical output is in um s-1 m-2. The LI-192 uses computer-tailored filter glass to achieve the desired quantum response. Calibration is traceable to NIST. The LI-192 serial numbers begin with UWQ-XXXXX. LI-COR has been producing Underwater Quantum Sensors since 1973.
These LI-192 sensors are typically listed as LI-192SA to designate the 2-pin connector on the base of the housing and require an Underwater Cable (LI-COR part number 2222UWB) to connect to the pins on the Sensor and connect to a data recording device.
The LI-192 differs from the LI-193 primarily in sensitivity and angular response.
193: Sensitivity: Typically 7 uA per 1000 umol s-1 m-2 in water. Azimuth: < ± 3% error over 360° at 90° from normal axis. Angular Response: < ± 4% error up to ± 90° from normal axis.
192: Sensitivity: Typically 4 uA per 1000 umol s-1 m-2 in water. Azimuth: < ± 1% error over 360° at 45° elevation. Cosine Correction: Optimized for underwater and atmospheric use.
(www.licor.com) Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0120/
Fibox 4 fiber optic oxygen system (PreSens)
Fibox 4 fiber optic oxygen system (PreSens)
PI Supplied Instrument Name: Fibox 4 fiber optic oxygen system (PreSens) Instrument Name: Oxygen Sensor Instrument Short Name:Dissolved Oxygen Sensor Instrument Description: An electronic device that measures the proportion of oxygen (O2) in the gas or liquid being analyzed
FLUOstar Omega plate reader (BMG Labtech, Germany)
FLUOstar Omega plate reader (BMG Labtech, Germany)
PI Supplied Instrument Name: FLUOstar Omega plate reader (BMG Labtech, Germany) Instrument Name: plate reader Instrument Short Name: Instrument Description: Plate readers (also known as microplate readers) are laboratory instruments designed to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. Sample reactions can be assayed in 6-1536 well format microtiter plates. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 uL per well. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µL per well. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization. From: http://en.wikipedia.org/wiki/Plate_reader, 2014-09-0-23.
Fluorescence Induction and Relaxation (FIRe) fluorometer (Satlantic Inc., Halifax)
Fluorescence Induction and Relaxation (FIRe) fluorometer (Satlantic Inc., Halifax)
PI Supplied Instrument Name: Fluorescence Induction and Relaxation (FIRe) fluorometer (Satlantic Inc., Halifax) Instrument Name: Satlantic Fluorescence Induction and Relaxation of Emission Spectrometer Instrument Short Name:Satlantic FIRe Instrument Description: The Satlantic FIRe (Fluorescence Induction and Relaxation) System is a bio-optical technology used to measure variable chlorophyll fluorescence in photosynthetic organisms. Based on the Fast Repetition Rate Fluorometry (FRRF) technique, the FIRe was developed in collaboration with Dr. Maxim Gorbunov and Dr. Paul Falkowski from Rutgers University.
More information on FIRe (PDF).