http://lod.bco-dmo.org/id/dataset/865053
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2021-11-16
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Effects of sample volume on N2O recovery and isotopic analysis (Biological Nitrogen Isotope Fractionation project)
2021-11-16
publication
2021-11-16
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2022-01-14
publication
https://doi.org/10.26008/1912/bco-dmo.865053.1
Julie Granger
University of Connecticut
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Zhou, M., Granger, J., Chang, B. X. (2022) Effects of sample volume on N2O recovery and isotopic analysis (Biological Nitrogen Isotope Fractionation project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-11-16 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.865053.1 [access date]
Effects of sample volume on N2O recovery and isotopic analysis Dataset Description: Methods and Sampling: <p><strong>Methodology:&nbsp;</strong></p>
<p><strong>Sampling and analytical procedures:&nbsp;</strong></p>
<p><u>
Effects of sample volume on N<sub>2</sub>O isotope analyses</u></p>
<p>Crimp-sealed vials (20 mL) containing incremental volumes (0 to 14 mL) of DIW or NO<sub>3</sub><sup>-</sup>-deplete seawater were sparged with N<sub>2</sub> gas for 30 minutes, aliquoted with N<sub>2</sub>O gas (10 nmol N) and equilibrated for ≥ 24 hours. The N<sub>2</sub>O gas in the vials was then extracted, purified and its N and O isotopic composition analyzed on the isotope ratio mass spectrometer. In a parallel set of experiments conducted at Princeton University, referring to Trial “Bonnie 5nmol”, crimp-sealed vials (20 mL) containing incremental volumes (0 to 9.3 mL) of DIW were sparged with helium gas for 30 minutes, aliquoted with N<sub>2</sub>O gas (5 nmol N) and equilibrated on a shaker for 2 hours. The N<sub>2</sub>O gas in the vials was then extracted with an autosampler needle that only penetrated samples ≥ 8.3 mL. N<sub>2</sub>O thus extracted was purified and its N and O isotopic composition analyzed on an isotope ratio mass spectrometer.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1554474 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1554474
completed
Julie Granger
University of Connecticut
860-405-9094
Department of Marine Sciences 1080 Shenecossett Road
Groton
CT
06340
USA
julie.granger@uconn.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Aliquot
Date
Trial
GC_IRMS
N2O_injection
Aliquot_volume
N2O_peak_area
delta_15N
delta_18O
Delta V Advantage and MAT253 continuous flow gas chromatograph isotope ratio mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA)
theme
None, User defined
sample type
date
replicate
No BCO-DMO term
Nitrite
volume
Nitrous Oxide
d15N
stable oxygen ration 18
featureType
BCO-DMO Standard Parameters
Gas Chromatograph Mass Spectrometer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
CAREER: The biological nitrogen isotope systematics of ammonium consumption and production
https://osprey.bco-dmo.org/project/856532
CAREER: The biological nitrogen isotope systematics of ammonium consumption and production
<p><strong>NSF Award Abstract:</strong><br />
The nitrogen (N) cycle in the marine environment is controlled by biological processes. Unfortunately, quantifying these processes and assessing their effect on the N cycle is difficult by direct measurements because of large spatial and temporal differences. Isotopic composition measurements of N provide a means to constrain these processes indirectly; however, there is still a great deal to be understood about isotope fractionation of recycled nitrogen through biological processes, which has made interpretation of novel nitrogen isotope data difficult. A researcher from the University of Connecticut plans to determine the influence of biological consumption and production on the isotope fractionation in ammonium. By helping to understand the processes surrounding fractionation of recycled ammonium at the organism level, this research will create a basis for which future researchers can better interpret isotope composition data to infer nitrogen cycle dynamics. A graduate student, a postdoctoral fellow, and two or more undergraduate students will be involved in the research. The researcher plans to integrate science with community-engaged learning by developing an undergraduate field and laboratory course that will require the students to present their research to stakeholders in the community. There will be a manual created for this course that will be disseminated in open-access forums for teachers hoping to develop similar courses.</p>
<p>Biological nitrogen isotope fractionation associated with nitrogen recycling remains poorly constrained despite the advent of a variety of new techniques to analyze nitrogen isotopes in recent years. The use of isotopic composition data can be incredibly useful to interpreting nitrogen cycle processes in the ocean that are difficult to measure directly, which makes it crucial to further understand the processes behind fractionation to catch up with the advancement of the datasets available to researchers. This research will characterize the isotope fractionation dynamics of ammonium during biological consumption and production. The researchers will investigate whether the characteristic low concentrations of ammonium in the surface ocean affect isotope fractionation when the ammonium is recycled and whether there is a trophic isotope effect associated with ammonium recycling by protozoan grazers. With this research, there will be a baseline from which researchers can interpret recycled nitrogen dynamics from ammonium isotope datasets. The methods of comparing nitrogen cycling studies will become significantly clearer with such a standard making interpretation uniform by removing significant uncertainties.</p>
Biological Nitrogen Isotope Fractionation
largerWorkCitation
project
eng; USA
oceans
2012-01-31
2020-08-24
0
BCO-DMO catalogue of parameters from Effects of sample volume on N2O recovery and isotopic analysis (Biological Nitrogen Isotope Fractionation project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/865582.rdf
Name: Aliquot
Units: unitless
Description: <p>Types of aliquot: freshwater or seawater</p>
http://lod.bco-dmo.org/id/dataset-parameter/865583.rdf
Name: Date
Units: unitless
Description: <p>Date of the experiments; yyyy-mm-dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/865584.rdf
Name: Trial
Units: unitless
Description: <p>Trial name</p>
http://lod.bco-dmo.org/id/dataset-parameter/865585.rdf
Name: GC_IRMS
Units: unitless
Description: <p>Gas chromatography combustion isotope ratio mass spectrometry (GC_IRMS) used for isotope analysis: Thermo Delta V at Uconn or Thermo MAT253 GC-IRMS at Princeton</p>
http://lod.bco-dmo.org/id/dataset-parameter/865586.rdf
Name: N2O_injection
Units: nmol of N
Description: <p>The amount of N2O gas injected</p>
http://lod.bco-dmo.org/id/dataset-parameter/865587.rdf
Name: Aliquot_volume
Units: mL
Description: <p>Volume of aliquot addition</p>
http://lod.bco-dmo.org/id/dataset-parameter/865588.rdf
Name: N2O_peak_area
Units: Vs
Description: <p>Recoverd N2O peak area measured with a Thermo Delta V GC-IRMS with modified Gas Bench II and a PAL autosampler</p>
http://lod.bco-dmo.org/id/dataset-parameter/865589.rdf
Name: delta_15N
Units: ‰ vs. N2Otank
Description: <p>N isotopic composition of N2O measured with a Thermo Delta V GC-IRMS with modified Gas Bench II and a PAL autosampler</p>
http://lod.bco-dmo.org/id/dataset-parameter/865590.rdf
Name: delta_18O
Units: ‰ vs. N2Otank
Description: <p>O isotopic composition of N2O measured with a Thermo Delta V GC-IRMS with modified Gas Bench II and a PAL autosampler</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
11736
https://darchive.mblwhoilibrary.org/bitstream/1912/27935/1/dataset-865053_incomplete-sparging__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.865053.1
download
onLine
dataset
<p><strong>Methodology:&nbsp;</strong></p>
<p><strong>Sampling and analytical procedures:&nbsp;</strong></p>
<p><u>
Effects of sample volume on N<sub>2</sub>O isotope analyses</u></p>
<p>Crimp-sealed vials (20 mL) containing incremental volumes (0 to 14 mL) of DIW or NO<sub>3</sub><sup>-</sup>-deplete seawater were sparged with N<sub>2</sub> gas for 30 minutes, aliquoted with N<sub>2</sub>O gas (10 nmol N) and equilibrated for ≥ 24 hours. The N<sub>2</sub>O gas in the vials was then extracted, purified and its N and O isotopic composition analyzed on the isotope ratio mass spectrometer. In a parallel set of experiments conducted at Princeton University, referring to Trial “Bonnie 5nmol”, crimp-sealed vials (20 mL) containing incremental volumes (0 to 9.3 mL) of DIW were sparged with helium gas for 30 minutes, aliquoted with N<sub>2</sub>O gas (5 nmol N) and equilibrated on a shaker for 2 hours. The N<sub>2</sub>O gas in the vials was then extracted with an autosampler needle that only penetrated samples ≥ 8.3 mL. N<sub>2</sub>O thus extracted was purified and its N and O isotopic composition analyzed on an isotope ratio mass spectrometer.</p>
Specified by the Principal Investigator(s)
<p><strong>Processing notes from submitting researcher:</strong></p>
<ul>
<li>Data were processed using Microsoft Excel</li>
</ul>
<p><strong>BCO-DMO processing notes</strong></p>
<ul>
<li>Date formats were changed from mm/dd/yy to yyyy-mm-dd</li>
<li>Spaces and units removed from column headers</li>
</ul>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Delta V Advantage and MAT253 continuous flow gas chromatograph isotope ratio mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA)
Delta V Advantage and MAT253 continuous flow gas chromatograph isotope ratio mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA)
PI Supplied Instrument Name: Delta V Advantage and MAT253 continuous flow gas chromatograph isotope ratio mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) PI Supplied Instrument Description:Delta V Advantage and MAT253 continuous flow gas chromatograph isotope ratio mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) interfaced with a modified Thermo Fisher Scientific Gas Bench sample preparation device fronted by dual cold traps (Casciotti et al., 2002) and a GC Pal autosampler (CTC Analytics, Zwingen, Switzerland) - to measure N and O isotope ratio of nitrate using the denitrified method. Instrument Name: Gas Chromatograph Mass Spectrometer Instrument Short Name: Instrument Description: Instruments separating gases, volatile substances or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay by a mass spectrometer.