http://lod.bco-dmo.org/id/dataset/865089
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2021-11-16
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Volume effects on blanks originating from the bacterial concentrates, equilibration with atmospheric N2O, and NO3- contamination of the water into which the standards were diluted from 2017-2020 (Biological Nitrogen Isotope Fractionation project)
2021-11-16
publication
2021-11-16
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2022-01-14
publication
https://doi.org/10.26008/1912/bco-dmo.865089.1
Julie Granger
University of Connecticut
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Zhou, M., Granger, J., Chang, B. X. (2022) Volume effects on blanks originating from the bacterial concentrates, equilibration with atmospheric N2O, and NO3- contamination of the water into which the standards were diluted from 2017-2020 (Biological Nitrogen Isotope Fractionation project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-11-16 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.865089.1 [access date]
Volume effects on blanks originating from the bacterial concentrates, equilibration with atmospheric N2O, and NO3- contamination of the water into which the standards were diluted Dataset Description: Methods and Sampling: <p><strong>Methodology:&nbsp;</strong></p>
<p><strong>Sampling and analytical procedures:&nbsp;</strong></p>
<p><u>
Effects of sample volume on the size of bacterial blanks</u></p>
<p>Following sparging with N<sub>2</sub> gas, bacterial concentrates (2 mL) in 20 mL vials were injected with incremental volumes of DIW or NO<sub>3</sub><sup>-</sup>-deplete surface Sargasso seawater. Solutions included air-equilibrated DIW (for ≥ 1 day) and helium-sparged DIW (for 30 minutes), as well as air-equilibrated vs. helium-sparged seawater. N<sub>2</sub>O yields were estimated from peak areas recovered by mass spectrometric analysis, calibrated with standard additions.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1554474 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1554474
completed
Julie Granger
University of Connecticut
860-405-9094
Department of Marine Sciences 1080 Shenecossett Road
Groton
CT
06340
USA
julie.granger@uconn.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Strain
Date
Trial
Solution
Sample_processiong
Sample_volume
Blank_N2O
isotope ratio mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA)
theme
None, User defined
species scientific name (binomial)
date
replicate
sample type
volume
Nitrous Oxide
featureType
BCO-DMO Standard Parameters
Gas Chromatograph Mass Spectrometer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
CAREER: The biological nitrogen isotope systematics of ammonium consumption and production
https://osprey.bco-dmo.org/project/856532
CAREER: The biological nitrogen isotope systematics of ammonium consumption and production
<p><strong>NSF Award Abstract:</strong><br />
The nitrogen (N) cycle in the marine environment is controlled by biological processes. Unfortunately, quantifying these processes and assessing their effect on the N cycle is difficult by direct measurements because of large spatial and temporal differences. Isotopic composition measurements of N provide a means to constrain these processes indirectly; however, there is still a great deal to be understood about isotope fractionation of recycled nitrogen through biological processes, which has made interpretation of novel nitrogen isotope data difficult. A researcher from the University of Connecticut plans to determine the influence of biological consumption and production on the isotope fractionation in ammonium. By helping to understand the processes surrounding fractionation of recycled ammonium at the organism level, this research will create a basis for which future researchers can better interpret isotope composition data to infer nitrogen cycle dynamics. A graduate student, a postdoctoral fellow, and two or more undergraduate students will be involved in the research. The researcher plans to integrate science with community-engaged learning by developing an undergraduate field and laboratory course that will require the students to present their research to stakeholders in the community. There will be a manual created for this course that will be disseminated in open-access forums for teachers hoping to develop similar courses.</p>
<p>Biological nitrogen isotope fractionation associated with nitrogen recycling remains poorly constrained despite the advent of a variety of new techniques to analyze nitrogen isotopes in recent years. The use of isotopic composition data can be incredibly useful to interpreting nitrogen cycle processes in the ocean that are difficult to measure directly, which makes it crucial to further understand the processes behind fractionation to catch up with the advancement of the datasets available to researchers. This research will characterize the isotope fractionation dynamics of ammonium during biological consumption and production. The researchers will investigate whether the characteristic low concentrations of ammonium in the surface ocean affect isotope fractionation when the ammonium is recycled and whether there is a trophic isotope effect associated with ammonium recycling by protozoan grazers. With this research, there will be a baseline from which researchers can interpret recycled nitrogen dynamics from ammonium isotope datasets. The methods of comparing nitrogen cycling studies will become significantly clearer with such a standard making interpretation uniform by removing significant uncertainties.</p>
Biological Nitrogen Isotope Fractionation
largerWorkCitation
project
eng; USA
oceans
2017-08-01
2020-06-08
0
BCO-DMO catalogue of parameters from Volume effects on blanks originating from the bacterial concentrates, equilibration with atmospheric N2O, and NO3- contamination of the water into which the standards were diluted from 2017-2020 (Biological Nitrogen Isotope Fractionation project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/865592.rdf
Name: Strain
Units: unitless
Description: <p>The two denitrifying bacteria strains used in the laboratory experiment: P. aureofaciens and P. chlororaphis</p>
http://lod.bco-dmo.org/id/dataset-parameter/865593.rdf
Name: Date
Units: unitless
Description: <p>Date of the experiments; yyyy-mm-dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/865594.rdf
Name: Trial
Units: unitless
Description: <p>Trial name</p>
http://lod.bco-dmo.org/id/dataset-parameter/865595.rdf
Name: Solution
Units: unitless
Description: <p>Type of aliquot. DIW or nitrate-deplete surface Sargasso seawater</p>
http://lod.bco-dmo.org/id/dataset-parameter/865596.rdf
Name: Sample_processiong
Units: unitless
Description: <p>Solutions were equilibrated in the air for ≥ 1 day, or sparged with helium or N2 gas for 30 minutes</p>
http://lod.bco-dmo.org/id/dataset-parameter/865597.rdf
Name: Sample_volume
Units: mL
Description: <p>Volume of solution addition</p>
http://lod.bco-dmo.org/id/dataset-parameter/865598.rdf
Name: Blank_N2O
Units: nmol of N
Description: <p>The amount of blank N2O recovered with a Thermo Delta V GC-IRMS with modified Gas Bench II and a PAL autosampler</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
12857
https://darchive.mblwhoilibrary.org/bitstream/1912/27936/1/dataset-865089_blank-sources__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.865089.1
download
onLine
dataset
<p><strong>Methodology:&nbsp;</strong></p>
<p><strong>Sampling and analytical procedures:&nbsp;</strong></p>
<p><u>
Effects of sample volume on the size of bacterial blanks</u></p>
<p>Following sparging with N<sub>2</sub> gas, bacterial concentrates (2 mL) in 20 mL vials were injected with incremental volumes of DIW or NO<sub>3</sub><sup>-</sup>-deplete surface Sargasso seawater. Solutions included air-equilibrated DIW (for ≥ 1 day) and helium-sparged DIW (for 30 minutes), as well as air-equilibrated vs. helium-sparged seawater. N<sub>2</sub>O yields were estimated from peak areas recovered by mass spectrometric analysis, calibrated with standard additions.</p>
Specified by the Principal Investigator(s)
<p><strong>Processing notes from submitting researcher:</strong></p>
<ul>
<li>Data were processed in Microsoft Excel</li>
</ul>
<p><strong>BCO-DMO processing notes</strong></p>
<ul>
<li>Date formats were changed from mm/dd/yy to yyyy-mm-dd</li>
<li>Spaces and units removed from column headers</li>
</ul>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
isotope ratio mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA)
isotope ratio mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA)
PI Supplied Instrument Name: isotope ratio mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) PI Supplied Instrument Description:Delta V Advantage continuous flow gas chromatograph isotope ratio mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) interfaced with a modified Thermo Fisher Scientific Gas Bench sample preparation device fronted by dual cold traps (Casciotti et al., 2002) and a GC Pal autosampler (CTC Analytics, Zwingen, Switzerland) - to measure N and O isotope ratio of nitrate using the denitrified method. Instrument Name: Gas Chromatograph Mass Spectrometer Instrument Short Name: Instrument Description: Instruments separating gases, volatile substances or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay by a mass spectrometer.