http://lod.bco-dmo.org/id/dataset/865893
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2021-12-07
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Dissolved and particulate carbon and nitrogen data from seawater collected during CCGS John P. Tully cruises in the northeast Pacific Ocean from Vancouver Island to Station P from 2018 to 2020
2021-12-07
publication
2021-12-07
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2021-12-20
publication
https://doi.org/10.26008/1912/bco-dmo.865893.1
Phoebe J. Lam
University of California-Santa Cruz
principalInvestigator
Andrea J. Fassbender
National Oceanic and Atmospheric Administration
principalInvestigator
Sophia Johannessen
Fisheries and Oceans Canada, Pacific Region
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Fassbender, A. J., Johannessen, S., Long, J. S., Wright, C. (2021) Dissolved and particulate carbon and nitrogen data from seawater collected during CCGS John P. Tully cruises in the northeast Pacific Ocean from Vancouver Island to Station P from 2018 to 2020. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-12-07 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.865893.1 [access date]
Methods and Sampling: <p>Sampling was conducted aboard the CCGS John P. Tully during five cruises (2018-2020) in the northeast Pacific from Vancouver Island to Station P (50°N, 145°W).&nbsp;<br />
Fisheries and Oceans Canada (DFO) Cruise numbers: 2018-40, 2019-001, 2019-006, 2019-008, 2020-001</p>
<p><strong><u>Particulate Nitrogen and Carbon</u></strong></p>
<p><strong>Cruise 2018-040:&nbsp;&nbsp;</strong>Prior to use, Whatman 25 mm GF/C filters (~1.2 μm pore size) were placed in aluminum foil packets and baked at 450°C for 4.5 hours and allowed to cool overnight, sealed, and stored in a zip-lock bags in plastic Tupperware containers previously cleaned with a dilute solution of RBS-35 (Thermo Scientific) in deionized water. Filters were not pre-weighed for this cruise. Particulate concentration was determined using the amount of water filtered.</p>
<p><strong>Cruises 2019-001, 2019-006, 2019-008, and 2020-001:&nbsp;&nbsp;</strong>Whatman 25 mm GF/F filters (~0.7 μm pore size) were used for all other cruises. Prior to use, GF/F filters were baked at 450°C for 4 hours and allowed to cool in the muffle furnace overnight. Further cooling took place in a desiccator. Once cooled to air temperature, filters were weighed on a Sartorius LE225B balance and then stored in individual PALL plastic 47mm filter holders until use.</p>
<p><strong>All Cruises:</strong><br />
For all cruises, all water bottles and silicone tubing used for sampling were initially cleaned in a dilute Extran solution and thoroughly rinsed with Type 1 deionized water. Water samples were collected from Niskin bottles directly into 330 mL to 1L HDPE bottles, through silicon tubing or, at times, directly from the Niskin. Sample bottles were rinsed three times with aliquots from the Niskin and then filled. Volumes varied depending on availability of bottles. Samples were filtered immediately. People doing the sampling&nbsp;wore either vinyl or nitrile gloves. Sample bottles/tubing were reused through the cruise and well-rinsed with sample water between Niskins/casts.</p>
<p>Pre-combusted filters were placed onto a filtration manifold and samples were filtered under vacuum at 5 mm Hg or less. For total particulate carbon, 0.2 μm filtered seawater was used as a final rinse down the sides of the filtration cups. For particulate organic carbon, acidified seawater (10% HCl) was used as a final rinse to purge off the carbonate.</p>
<p>Cleaned forceps were used to remove filters from the manifold and returned to their original containers. After sampling, the filters were oven dried at 30°C. Filters were then stored at -80°C until returned to the lab where they were dried at 30°C for 24 hours. Samples were cooled in a dessicator. They were then sent to Stanford University for further chemical analysis. Samples that arrived at Stanford were placed into plastic petri dishes.</p>
<p>At Stanford University, filters from all cruises were loaded into a Costech Analytical Technologies Zero Blank carousel attached to a Carlo Erba NA1500 Series 2 elemental analyzer (EA) at the Stanford University Stable Isotope Laboratory Facility. The EA was configured with a 10 ml oxygen loop, a combustion oxidation reactor set at 1080°C, reduction reactor at 650°C, magnesium perchlorate column to remove water, the chromatography column set to 60°C, and the thermal conductivity detector set at 190°C. The UHP grade helium flow rate was 65 ml per minute&nbsp;and the UHP oxygen used to enhance combustion was 20 ml per minute.&nbsp;</p>
<p>All standards were loaded into 5×9 mm tin capsules (Costech Analytical Technologies). An empty 5×9 mm tin was analyzed with each run sequence for blank correction. All GF/C and GF/F filters were loaded into 9×10 mm tin capsules and one pre-combusted GF/C or GF/F filter was loaded into a 9×10 mm tin capsule for blank correction. Each 31-drop run sequence was composed of 2 conditioners, 1 5×9 mm tin blank, 1 9×10 mm filter blank, 2 acetanilide standards, 7 unknown filters, 1 acetanilide standard, 7 unknown filters, 1 acetanilide standard, 7 unknown filters, 2 acetanilide standards.</p>
<p>Though instrument precision is relatively high (± 1 µg N, and ± 2 µg C), overall uncertainty is strongly tied to the sampling procedure, with errors anticipated to range from 10% to 25%.</p>
<p><br />
<strong><u>Dissolved Inorganic Carbon, Total Alkalinity, and pH</u></strong><br />
Samples were collected following standard protocols (Dickson et al., 2007) into 500 mL borosilicate glass bottles and preserved with 200 μL saturated mercuric chloride for later analysis.&nbsp;Samples were shipped to and analyzed at the Monterey Bay Aquarium Research Institute (MBARI). Three parameters (DIC, TA, and pH) were measured from each 500 mL sample bottle. All samples were run in triplicate, and the results were averaged. DIC and TA instrument performance was monitored by measuring Certified Reference Material (CRM; provided by Andrew Dickson at SIO) approximately hourly.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2032754 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2032754
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1756932 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1756932
completed
Phoebe J. Lam
University of California-Santa Cruz
831-459-4587
1156 High St. Department of Ocean Sciences
Santa Cruz
CA
95064
USA
pjlam@ucsc.edu
pointOfContact
Andrea J. Fassbender
National Oceanic and Atmospheric Administration
509-438-4961
7600 Sand Point Way N.E.
Seattle
WA
98115
USA
andrea.j.fassbender@noaa.gov
pointOfContact
Sophia Johannessen
Fisheries and Oceans Canada, Pacific Region
(250) 363-6616
Institute of Ocean Sciences (Fisheries and Oceans Canada) 9860 W. Saanich Rd., P.O. Box 6000
Sidney
British Columbia
V8L 5P4
Canada
Sophia.johannessen@dfo-mpo.gc.ca
pointOfContact
asNeeded
Dataset Version: 1
Unknown
ISO_DateTime_UTC
Vessel
Cruise_ID
Event_Number
Station
Niskin
Latitude
Longitude
Depth
PON_GFF
POC_GFF
QF_GFF_PO
Comments_GFF_PO
PON_GFC
POC_GFC
QF_GFC_PO
Comments_GFC_PO
TPN_GFF
TPC_GFF
QF_GFF_TP
Comments_GFF_TP
TPN_GFC
TPC_GFC
QF_GFC_TP
Comments_GFC_TP
DIC
DIC_flag
TA
TA_flag
R
pH_20_deg
pH_in_situ
pH_flag
Carlo Erba NA1500 Series 2 elemental analyzer
LiCOR 7000 Nondispersive Infrared gas analyzer
Niskin bottle
Kloehn V6 syringe pump
Sartorius LE225B balance
Agilent 8453 spectrometer
Metrohm 855 automated titrator
theme
None, User defined
ISO_DateTime_UTC
ship
Cruise identfier
event
station
Niskin bottle number
latitude
longitude
depth
particulate organic nitrogen
particulate organic Carbon (POC)
quality flag
comments
Total Particulate Nitrogen
total particulate Carbon
dissolved inorganic Carbon
total alkalinity (TA)
ratio
pH
featureType
BCO-DMO Standard Parameters
Carlo-Erba NA-1500 Elemental Analyzer
LI-COR LI-7000 Gas Analyzer
Niskin bottle
Pump
scale or balance
Spectrophotometer
Titrator
instrument
BCO-DMO Standard Instruments
Line-P_cruises
service
Deployment Activity
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Constraining Upper-Ocean Carbon Export with Biogeochemical Profiling Floats
https://osprey.bco-dmo.org/project/838069
Constraining Upper-Ocean Carbon Export with Biogeochemical Profiling Floats
<p>OCE-1756932 Start Date: 2018-03-01<br />
OCE-2032754 Start Date: 2020-05-28</p>
<p><strong>NSF abstract:</strong><br />
A goal in chemical oceanography is to advance our understanding of the global carbon cycle, specifically to quantify the transfer of carbon from the surface ocean to depth through the sinking of particles produced by marine organisms. Yet, modern global estimates of this process (commonly called carbon export) differ by over 100%. These estimates are often derived from regional relationships between ocean measurements and satellite observations that are then applied globally. Persistent differences between the satellite and field-based estimates of carbon export have been found throughout the ocean, suggesting that improvements are needed. This project will determine whether profiling floats equipped with chemical sensors can be used to estimate the export of carbon in the ocean. Floats will be deployed at Ocean Station Papa, but the approach is scalable in nature and could be used to validate and improve the satellite algorithms used for global carbon export determinations. The project will support a female, early career scientist and a postdoc, as well as facilitate international collaboration with Canadian scientists. Additionally, the results may assist the National Aeronautics and Space Administration (NASA) EXPORTS campaign as well as other satellite carbon export development efforts.</p>
<p>Modern global estimates of the biological pump differ by over 100% (~5 to >12 Pg C yr-1) making it challenging to determine the role of marine biogeochemical (BGC) cycling in modern climate and climate variability. Global carbon export estimates are often derived from regional empirical relationships between field and satellite observations that are then applied globally. Persistent discrepancies between unique satellite algorithms and unique geochemical approaches suggest that accurately quantifying the biological pump remains a fundamental research goal. This project will assess the capability of using BGC profiling floats to estimate the export of distinct biogenic carbon pools (dissolved and particulate organic carbon, and particulate inorganic carbon). By using BGC floats to close multiple upper ocean tracer budgets this project will address two known issues common to other geochemical approaches: assumptions about (1) dissolved organic carbon cycling and (2) the integration depth used for annual carbon export assessments. The method will be tested at Ocean Station Papa, but is scalable in nature and could be used to develop a carbon export database suitable for the validation and training of satellite algorithms required for global carbon export determinations. Results from the floats will be compared to satellite carbon export algorithm estimates over the 5-year float lifetimes. Ten years of existing BGC data from profiling floats and a mooring in the region will also be used to provide further context about interannual variability.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
EXPORTS BGC Floats
largerWorkCitation
project
eng; USA
oceans
-145.0008
-139.6662
49.63567
50.613
2018-09-21
2020-02-16
Eastern Subarctic Pacific near Ocean Station Papa (50°N, 145°W)
0
BCO-DMO catalogue of parameters from Dissolved and particulate carbon and nitrogen data from seawater collected during CCGS John P. Tully cruises in the northeast Pacific Ocean from Vancouver Island to Station P from 2018 to 2020
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/866316.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>Date and time in ISO8601 standard format (YYYY-MM-DDThh:mm:ssZ)</p>
http://lod.bco-dmo.org/id/dataset-parameter/866317.rdf
Name: Vessel
Units: unitless
Description: <p>Vessel</p>
http://lod.bco-dmo.org/id/dataset-parameter/866318.rdf
Name: Cruise_ID
Units: unitless
Description: <p>Cruise</p>
http://lod.bco-dmo.org/id/dataset-parameter/866319.rdf
Name: Event_Number
Units: unitless
Description: <p>Event number</p>
http://lod.bco-dmo.org/id/dataset-parameter/866320.rdf
Name: Station
Units: unitless
Description: <p>Station number</p>
http://lod.bco-dmo.org/id/dataset-parameter/866321.rdf
Name: Niskin
Units: unitless
Description: <p>Niskin bottle number</p>
http://lod.bco-dmo.org/id/dataset-parameter/866322.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitude of sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/866323.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitude of sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/866324.rdf
Name: Depth
Units: meters (m)
Description: <p>Depth of sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/866325.rdf
Name: PON_GFF
Units: micrograms per liter (ug N/L)
Description: <p>Particulate Organic Nitrogen sampled using GF/F filter. Instrument precision ± 1 µg N. Reference standard is acetanilide from Costech Analytical</p>
http://lod.bco-dmo.org/id/dataset-parameter/866326.rdf
Name: POC_GFF
Units: micrograms per liter (ug C/L)
Description: <p>Particulate Organic Nitrogen sampled using GF/F filter. Instrument precision ± 2 µg C. Reference standard is acetanilide from Costech Analytical</p>
http://lod.bco-dmo.org/id/dataset-parameter/866327.rdf
Name: QF_GFF_PO
Units: unitless
Description: <p>Quality flag for particulate organics on GF/F filter where 2=acceptable; 3=questionable; 4=bad; 5=not reported; 6=mean of replicates; 9=not sampled</p>
http://lod.bco-dmo.org/id/dataset-parameter/866328.rdf
Name: Comments_GFF_PO
Units: unitless
Description: <p>Comments for particulate organic samples on GF/F filters</p>
http://lod.bco-dmo.org/id/dataset-parameter/866329.rdf
Name: PON_GFC
Units: micrograms per liter (ug N/L)
Description: <p>Particulate Organic Nitrogen sampled using GF/C filter. Instrument precision ± 1 µg N. Reference standard is acetanilide from Costech Analytical</p>
http://lod.bco-dmo.org/id/dataset-parameter/866330.rdf
Name: POC_GFC
Units: micrograms per liter (ug C/L)
Description: <p>Particulate Organic Carbon sampled using GF/C filter. Instrument precision ± 2 µg C. Reference standard is acetanilide from Costech Analytical</p>
http://lod.bco-dmo.org/id/dataset-parameter/866331.rdf
Name: QF_GFC_PO
Units: unitless
Description: <p>Quality flag for particulate organics on GF/C filter where 2=acceptable; 3=questionable; 4=bad; 5=not reported; 6=mean of replicates; 9=not sampled</p>
http://lod.bco-dmo.org/id/dataset-parameter/866332.rdf
Name: Comments_GFC_PO
Units: unitless
Description: <p>Comments for particulate organic samples on GF/C filters</p>
http://lod.bco-dmo.org/id/dataset-parameter/866333.rdf
Name: TPN_GFF
Units: micrograms per liter (ug N/L)
Description: <p>Total Particulate Nitrogen sampled using GF/F filter. Instrument precision ± 1 µg N. Reference standard is acetanilide from Costech Analytical</p>
http://lod.bco-dmo.org/id/dataset-parameter/866334.rdf
Name: TPC_GFF
Units: micrograms per liter (ug C/L)
Description: <p>Total Particulate Carbon sampled using GF/F filter. Instrument precision ± 2 µg C. Reference standard is acetanilide from Costech Analytical</p>
http://lod.bco-dmo.org/id/dataset-parameter/866335.rdf
Name: QF_GFF_TP
Units: unitless
Description: <p>Quality flag for total particulates on GF/F filter where 2=acceptable; 3=questionable; 4=bad; 5=not reported; 6=mean of replicates; 9=not sampled</p>
http://lod.bco-dmo.org/id/dataset-parameter/866336.rdf
Name: Comments_GFF_TP
Units: unitless
Description: <p>Comments for total particulate samples on GF/F filter</p>
http://lod.bco-dmo.org/id/dataset-parameter/866337.rdf
Name: TPN_GFC
Units: micrograms per liter (ug N/L)
Description: <p>Total Particulate Nitrogen sampled using GF/C filter. Instrument precision ± 1 µg N. Reference standard is acetanilide from Costech Analytical</p>
http://lod.bco-dmo.org/id/dataset-parameter/866338.rdf
Name: TPC_GFC
Units: micrograms per liter (ug C/L)
Description: <p>Total Particulate Carbon sampled using GF/C filter. Instrument precision ± 2 µg C. Reference standard is acetanilide from Costech Analytical</p>
http://lod.bco-dmo.org/id/dataset-parameter/866339.rdf
Name: QF_GFC_TP
Units: unitless
Description: <p>Quality flag for total particulates on GF/C filter</p>
http://lod.bco-dmo.org/id/dataset-parameter/866340.rdf
Name: Comments_GFC_TP
Units: unitless
Description: <p>Comments for total particulate samples on GF/C filter</p>
http://lod.bco-dmo.org/id/dataset-parameter/866341.rdf
Name: DIC
Units: micromoles per kilogram (umols/kg)
Description: <p>Dissolved Inorganic Carbon where measurement precision is ± 1.6 µmol per kg. Certified Reference Material provided by Andrew Dickson at SIO.</p>
http://lod.bco-dmo.org/id/dataset-parameter/866342.rdf
Name: DIC_flag
Units: unitless
Description: <p>Quality flag for DIC where 2=acceptable; 3=questionable; 4=bad; 5=not reported; 6=mean of replicates; 9=not sampled</p>
http://lod.bco-dmo.org/id/dataset-parameter/866343.rdf
Name: TA
Units: micromoles per kilogram (umols/kg)
Description: <p>Total Alkalinity where measurement precision is ± 2.1 µmol per kg. Certified Reference Material provided by Andrew Dickson at SIO</p>
http://lod.bco-dmo.org/id/dataset-parameter/866344.rdf
Name: TA_flag
Units: unitless
Description: <p>Quality flag for Total Alkalinity where 2=acceptable; 3=questionable; 4=bad; 5=not reported; 6=mean of replicates; 9=not sampled</p>
http://lod.bco-dmo.org/id/dataset-parameter/866345.rdf
Name: R
Units: unitless
Description: <p>Ratio of spectrophotometric absorbance at 578 nm versus 434 nm. Instrument wavelength accuracy is ± 0.5 nm</p>
http://lod.bco-dmo.org/id/dataset-parameter/866346.rdf
Name: pH_20_deg
Units: unitless
Description: <p>Total scale pH measured at 20 degrees Celsius. Measurement precision is ± 0.005</p>
http://lod.bco-dmo.org/id/dataset-parameter/866347.rdf
Name: pH_in_situ
Units: unitless
Description: <p>Total scale pH converted to in situ temperature from 20 degrees Celsius measurement</p>
http://lod.bco-dmo.org/id/dataset-parameter/866348.rdf
Name: pH_flag
Units: unitless
Description: <p>Quality flag for pH where 2=acceptable; 3=questionable; 4=bad; 5=not reported; 6=mean of replicates; 9=not sampled</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
46196
https://darchive.mblwhoilibrary.org/bitstream/1912/27837/1/dataset-865893_discrete-c-and-n-near-station-p__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.865893.1
download
onLine
dataset
<p>Sampling was conducted aboard the CCGS John P. Tully during five cruises (2018-2020) in the northeast Pacific from Vancouver Island to Station P (50°N, 145°W).&nbsp;<br />
Fisheries and Oceans Canada (DFO) Cruise numbers: 2018-40, 2019-001, 2019-006, 2019-008, 2020-001</p>
<p><strong><u>Particulate Nitrogen and Carbon</u></strong></p>
<p><strong>Cruise 2018-040:&nbsp;&nbsp;</strong>Prior to use, Whatman 25 mm GF/C filters (~1.2 μm pore size) were placed in aluminum foil packets and baked at 450°C for 4.5 hours and allowed to cool overnight, sealed, and stored in a zip-lock bags in plastic Tupperware containers previously cleaned with a dilute solution of RBS-35 (Thermo Scientific) in deionized water. Filters were not pre-weighed for this cruise. Particulate concentration was determined using the amount of water filtered.</p>
<p><strong>Cruises 2019-001, 2019-006, 2019-008, and 2020-001:&nbsp;&nbsp;</strong>Whatman 25 mm GF/F filters (~0.7 μm pore size) were used for all other cruises. Prior to use, GF/F filters were baked at 450°C for 4 hours and allowed to cool in the muffle furnace overnight. Further cooling took place in a desiccator. Once cooled to air temperature, filters were weighed on a Sartorius LE225B balance and then stored in individual PALL plastic 47mm filter holders until use.</p>
<p><strong>All Cruises:</strong><br />
For all cruises, all water bottles and silicone tubing used for sampling were initially cleaned in a dilute Extran solution and thoroughly rinsed with Type 1 deionized water. Water samples were collected from Niskin bottles directly into 330 mL to 1L HDPE bottles, through silicon tubing or, at times, directly from the Niskin. Sample bottles were rinsed three times with aliquots from the Niskin and then filled. Volumes varied depending on availability of bottles. Samples were filtered immediately. People doing the sampling&nbsp;wore either vinyl or nitrile gloves. Sample bottles/tubing were reused through the cruise and well-rinsed with sample water between Niskins/casts.</p>
<p>Pre-combusted filters were placed onto a filtration manifold and samples were filtered under vacuum at 5 mm Hg or less. For total particulate carbon, 0.2 μm filtered seawater was used as a final rinse down the sides of the filtration cups. For particulate organic carbon, acidified seawater (10% HCl) was used as a final rinse to purge off the carbonate.</p>
<p>Cleaned forceps were used to remove filters from the manifold and returned to their original containers. After sampling, the filters were oven dried at 30°C. Filters were then stored at -80°C until returned to the lab where they were dried at 30°C for 24 hours. Samples were cooled in a dessicator. They were then sent to Stanford University for further chemical analysis. Samples that arrived at Stanford were placed into plastic petri dishes.</p>
<p>At Stanford University, filters from all cruises were loaded into a Costech Analytical Technologies Zero Blank carousel attached to a Carlo Erba NA1500 Series 2 elemental analyzer (EA) at the Stanford University Stable Isotope Laboratory Facility. The EA was configured with a 10 ml oxygen loop, a combustion oxidation reactor set at 1080°C, reduction reactor at 650°C, magnesium perchlorate column to remove water, the chromatography column set to 60°C, and the thermal conductivity detector set at 190°C. The UHP grade helium flow rate was 65 ml per minute&nbsp;and the UHP oxygen used to enhance combustion was 20 ml per minute.&nbsp;</p>
<p>All standards were loaded into 5×9 mm tin capsules (Costech Analytical Technologies). An empty 5×9 mm tin was analyzed with each run sequence for blank correction. All GF/C and GF/F filters were loaded into 9×10 mm tin capsules and one pre-combusted GF/C or GF/F filter was loaded into a 9×10 mm tin capsule for blank correction. Each 31-drop run sequence was composed of 2 conditioners, 1 5×9 mm tin blank, 1 9×10 mm filter blank, 2 acetanilide standards, 7 unknown filters, 1 acetanilide standard, 7 unknown filters, 1 acetanilide standard, 7 unknown filters, 2 acetanilide standards.</p>
<p>Though instrument precision is relatively high (± 1 µg N, and ± 2 µg C), overall uncertainty is strongly tied to the sampling procedure, with errors anticipated to range from 10% to 25%.</p>
<p><br />
<strong><u>Dissolved Inorganic Carbon, Total Alkalinity, and pH</u></strong><br />
Samples were collected following standard protocols (Dickson et al., 2007) into 500 mL borosilicate glass bottles and preserved with 200 μL saturated mercuric chloride for later analysis.&nbsp;Samples were shipped to and analyzed at the Monterey Bay Aquarium Research Institute (MBARI). Three parameters (DIC, TA, and pH) were measured from each 500 mL sample bottle. All samples were run in triplicate, and the results were averaged. DIC and TA instrument performance was monitored by measuring Certified Reference Material (CRM; provided by Andrew Dickson at SIO) approximately hourly.</p>
Specified by the Principal Investigator(s)
<p><strong><u>Particulate Nitrogen and Carbon</u></strong><br />
Analyses were conducted using a Costech Analytical Technologies Zero Blank carousel attached to a Carlo Erba NA1500 Series 2 elemental analyzer (EA). The instrument precision was estimated from Acetanilide standards (189 measurements over 24 unique sampling dates) as the standard deviation of weight percent measurements for both carbon and nitrogen multiplied by the mean total weight.</p>
<p>The data obtained from the Carlo Erba NA1500 Series 2 elemental analyzer were calibrated using Acetanilide standard composed of 10.36 Wt. % N and 71.09 Wt. % C.</p>
<p>K-factor (KF) method was used to correct all unknown data using 6 acetanilide standards using the following equations.<br />
Where:&nbsp;&nbsp; KF = (mass mg * 10.36)/(Area N – Area N blank)<br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; KF = (mass mg * 70.09)/(Area C – Area C blank)</p>
<p>Sample C and N concentrations were calculated as follows:&nbsp;<br />
Where:&nbsp;&nbsp;&nbsp;μg/L N = {(Average KF * (Area N – Area N blank))/Volume Filtered (L)} x (1000&nbsp;μg / 1 mg)<br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; μg/L C = {(Average KF * (Area C – Area C blank))/Volume Filtered (L)}<br />
&nbsp;</p>
<p><strong><u>Dissolved Inorganic Carbon (DIC)</u></strong><br />
DIC was analyzed using a custom automated system in which a Kloehn V6 syringe pump (5 mL syringe) handles fluid control, delivering 1.75 mL of sample to a custom designed CO<sub>2</sub> stripping chamber. 200 µL of 5% phosphoric acid (H<sub>3</sub>PO<sub>4</sub>) is subsequently added to the CO<sub>2</sub><sub> </sub>stripping chamber for acidification of the sample. CO<sub>2</sub>-free gas is then bubbled through the acidified sample and the evolved CO<sub>2</sub>(g) is delivered to a LiCOR 7000 Nondispersive Infrared gas analyzer for measurement. Carrier gas flow rate is controlled using a mass flow controller. The DIC concentration of the sample is proportional to the integral of the LiCOR CO<sub>2</sub>(g) sample peak. The LiCOR 7000 is calibrated annually using WMO traceable standard gases containing known CO<sub>2 </sub>mole fractions. The average accuracy of the instrument relative to CRMs is better than than 1μmol kg<sup>-1</sup>.</p>
<p>Sampling precision (σ<sub>s</sub>) was estimated by analyzing sets of replicate samples drawn from the same Niskin bottle during rosette casts. The average standard deviation between replicate sets was found to be ±1.5 μmol kg<sup>-1</sup> (n = 8).&nbsp;&nbsp;Analytical precision (σ<sub>i</sub>) was estimated as the standard deviation of CRM samples, post calibration factor implementation, analyzed intermittently with the cruise samples over the course of a few days. The analytical precision was found to be ±0.7 μmol kg<sup>-1</sup> (n = 23).&nbsp;The overall measurement precision of 1.6 μmol kg<sup>-1</sup> was&nbsp;estimated by combining the sampling precision and analytical precision&nbsp;[(σ<sub>s</sub><sup>2</sup>&nbsp;+ σ<sub>i</sub><sup>2</sup>)<sup>0.5</sup>], which is the square root of the sum of the squares.&nbsp;</p>
<p><strong><u>Total Alkalinity (TA)</u></strong><br />
Total Alkalinity was analyzed using a Metrohm 855 automated titrator following standard open cell alkalinity titration procedures. The titrant was comprised of 0.1 M hydrochloric acid (HCl) in a 0.7 M sodium chloride (NaCl) background solution. The titration temperature was held constant at 20.0 ± 0.2°C throughout the titration. The temperature of the sample was measured immediately upon delivery to the jacketed cell for later sample mass determination using density and volume. The average accuracy of the instrument relative to Certified Reference Material&nbsp;(CRM)&nbsp;is better than 1 μmol kg<sup>-1</sup>.&nbsp;</p>
<p>Sampling precision (σ<sub>s</sub>) was estimated by analyzing sets of replicate samples drawn from the same Niskin bottle during rosette casts. The average standard deviation between replicate sets was found to be ±1.5 μmol kg<sup>-1</sup> (n = 8).&nbsp; Analytical precision (σ<sub>i</sub>) was estimated as the standard deviation of CRM samples, post calibration factor implementation, analyzed intermittently with the cruise samples over the course of a few days. The analytical precision was found to be ±1.4 μmol kg<sup>-1</sup>&nbsp;(n = 13). The overall measurement precision of 2.1 μmol kg<sup>-1</sup>&nbsp;was estimated by combining the sampling precision and analytical precision [(σ<sub>s</sub><sup>2</sup>&nbsp;+ σ<sub>i</sub><sup>2</sup>)<sup>0.5</sup>], which is&nbsp;the square root of the sum of the squares.&nbsp;</p>
<p><strong><u>pH</u></strong><br />
pH was analyzed using an Agilent 8453 spectrometer in an automated system designed after the one described in&nbsp;<em>Carter et al.</em>, (2013). The temperature of the sample was held constant at 20°C using a 10-cm jacketed cell, and every sample was immersed in a 20°C water bath for at least 25 minutes before analysis. An indicator dye (purified m-cresol purple from Sunburst Sensors - lab batch 6) solution (2 mM) was used to assess sample pH. The sample pH perturbation caused by dye addition was quantified by adding both the normal amount and twice the amount of dye to seawater solutions of ~pH 7.4, 7.8, and 8.1. The pH perturbation caused by dye addition to the sample was quantified by adding both the normal amount and twice the amount of dye to a subset of seawater samples.&nbsp;The average precision of the instrument is ±0.004.&nbsp;</p>
<p>Sampling precision (σ<sub>s</sub>) was estimated by analyzing sets of replicate samples drawn from the same Niskin bottle during rosette casts. The average standard deviation between replicate sets was found to be ± 0.0031 (n = 9). Instrument precision (σ<sub>i</sub>) is estimated to be ±0.004. The overall measurement precision of 0.005 was estimated by combining the sampling precision and instrument precision&nbsp;&nbsp;[(σ<sub>s</sub><sup>2</sup>&nbsp;+ σ<sub>i</sub><sup>2</sup>)<sup>0.5</sup>],&nbsp;which is&nbsp;the square root of the sum of the squares.&nbsp;</p>
<p>=================<br />
<strong>BCO-DMO Processing:</strong></p>
<ul>
<li>Imported data from source file "LineP_QCd_bottle_data.xlsx" into the BCO-DMO data system.</li>
<li>Data file imported using missing data identifiers "NaN”.&nbsp;The missing data identifier "NaN" in the original source file will be displayed as appropriate based on the type of file you download from the BCO-DMO data system. For example, missing data will be shown as blank (null) values in the csv files. In MATLAB .mat files it will be displayed as NaN. When viewing data at BCO-DMO the missing value will be shown as "nd" meaning "no data."</li>
<li>Adjusted dates to be four digit years, and padded time column with leading zeros.&nbsp; Then converted separate date and time to single datetime column with ISO8601 format.&nbsp;&nbsp;</li>
<li>Added column for vessel/ship</li>
<li>Added conventional header with dataset name, PI name, version date.</li>
<li>Modified parameter (column) names to conform with BCO-DMO naming conventions. (The only allowed characters are A-Z,a-z,0-9, and underscores. No spaces, hyphens, commas, parentheses, or Greek letters).</li>
</ul>
<p>&nbsp;</p>
<p>&nbsp;</p>
<p>&nbsp;</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Carlo Erba NA1500 Series 2 elemental analyzer
Carlo Erba NA1500 Series 2 elemental analyzer
PI Supplied Instrument Name: Carlo Erba NA1500 Series 2 elemental analyzer PI Supplied Instrument Description:Particulate nitrogen and carbon were measured using a Costech Analytical Technologies Zero Blank carousel attached to a Carlo Erba NA1500 Series 2 elemental analyzer Instrument Name: Carlo-Erba NA-1500 Elemental Analyzer Instrument Short Name:Carlo-Erba NA-1500 Instrument Description: A laboratory instrument that simultaneously determines total nitrogen and total carbon from a wide range of organic and inorganic sediment samples. The sample is completely and instantaneously oxidised by flash combustion, which converts all organic and inorganic substances into combustion products. The resulting combustion gases pass through a reduction furnace and are swept into the chromatographic column by the carrier gas which is helium. The gases are separated in the column and detected by the thermal conductivity detector which gives an output signal proportional to the concentration of the individual components of the mixture. The instrument was originally manufactured by Carlo-Erba, which has since been replaced by Thermo Scientific (part of Thermo Fisher Scientific). This model is no longer in production. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0470/
LiCOR 7000 Nondispersive Infrared gas analyzer
LiCOR 7000 Nondispersive Infrared gas analyzer
PI Supplied Instrument Name: LiCOR 7000 Nondispersive Infrared gas analyzer PI Supplied Instrument Description:The evolved CO2 gas is delivered to a LiCOR 7000 Nondispersive Infrared gas analyzer for measurement.
The LiCOR 7000 is calibrated annually using WMO traceable standard gases containing known CO2 mole fractions.
The average accuracy of the instrument relative to CRMs is better than 1 μmol per kilogram. Instrument Name: LI-COR LI-7000 Gas Analyzer Instrument Short Name:LI-COR LI-7000 Instrument Description: The LI-7000 gas analyzer is a differential, single source, non-dispersive, infrared gas analyzer. It has two solid state detectors, one each for CO2 and H2O, filters at 4.255 microns and 2.595 microns respectively. CO2 is measured in the range 0-3000ppm, with an accuracy of 1 percent nominally. H2O is measured in the range 0-60 mmol per mol, with an accuracy of one 1 percent. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/382/
Niskin bottle
Niskin bottle
PI Supplied Instrument Name: Niskin bottle PI Supplied Instrument Description:Seawater samples for dissolved and total organic carbon were collected from 10 L Niskin bottles Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
Kloehn V6 syringe pump
Kloehn V6 syringe pump
PI Supplied Instrument Name: Kloehn V6 syringe pump PI Supplied Instrument Description:A custom analysis system for DIC was used in which a Kloehn V6 syringe pump (5 mL syringe) handles fluid control. Instrument Name: Pump Instrument Short Name: Instrument Description: A pump is a device that moves fluids (liquids or gases), or sometimes slurries, by mechanical action. Pumps can be classified into three major groups according to the method they use to move the fluid: direct lift, displacement, and gravity pumps
Sartorius LE225B balance
Sartorius LE225B balance
PI Supplied Instrument Name: Sartorius LE225B balance PI Supplied Instrument Description:Filters were weighed on a Sartorius LE225B balance Instrument Name: scale or balance Instrument Short Name: Instrument Description: Devices that determine the mass or weight of a sample. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB13/
Agilent 8453 spectrometer
Agilent 8453 spectrometer
PI Supplied Instrument Name: Agilent 8453 spectrometer PI Supplied Instrument Description:Agilent 8453 UV-visible Spectroscopy System (aka Agilent 8453 spectrophotometer) has average precision of ±0.004. Instrument Name: Spectrophotometer Instrument Short Name:Spectrophotometer Instrument Description: An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/
Metrohm 855 automated titrator
Metrohm 855 automated titrator
PI Supplied Instrument Name: Metrohm 855 automated titrator PI Supplied Instrument Description:The average accuracy of the Metrohm 855 automated titrator relative to Certified Reference Materials is better than 1μmol per kilogram. Instrument Name: Titrator Instrument Short Name:Titrator Instrument Description: Titrators are instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB12/
Cruise: Line-P_cruises
Line-P_cruises
Community Standard Description
International Council for the Exploration of the Sea
CCGS John P. Tully
vessel
Community Standard Description
International Council for the Exploration of the Sea
CCGS John P. Tully
vessel