http://lod.bco-dmo.org/id/dataset/866359
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2021-12-08
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Mesocosm study of trophic interactions under ocean acidification, focusing on the consumption of algae by snails in Bodega Bay, California
2022-03-09
publication
2022-03-09
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2022-03-16
publication
https://doi.org/10.26008/1912/bco-dmo.866359.1
Brian Gaylord
University of California - Davis: Bodega Marine Laboratory
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Jellison, B., Gaylord, B. (2022) Mesocosm study of trophic interactions under ocean acidification, focusing on the consumption of algae by snails in Bodega Bay, California. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-03-09 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.866359.1 [access date]
Methods and Sampling: <p>This dataset is part of a larger experiment to investigate how pH influences trophic links between intertidal sea stars (<em>Leptasterias hexactis</em>), snails (<em>Tegula funebralis</em>), and macroalgae (<em>Mazzaella flaccida)</em>. These data were gathered during an experiment at the Bodega Marine Laboratory. Wet weight (hand blotted; grams) of algae was determined before and after the experiment using an analytical balance (Mettler AE 160).</p>
<p>Organisms were placed for 7 days in mesocosms containing seawater at either ambient (~7.9) or low pH (~7.0). The pH was modified using equimolar additions of sodium bicarbonate (NaHCO3) and hydrochloric acid (HCl). The water in each container was changed daily. The mesocosm array consisted of 40, 13-liter (L) circular plastic containers with a mesh barrier down the center to separate predator, prey, and/or basal resource but allowing for passage of waterborne cue. Mesocosms were filled halfway with seawater, allowing 10 centimeters (cm) of refuge space for snails above the waterline. Mesocosms were held within a seawater table under constant flow to maintain consistent temperatures.</p>
<p>Each mesocosm was assigned to one of four trophic treatments and one of two pH levels, resulting in five replicates per treatment and pH (4 trophic × 2 pH × 5 replicates = 40 mesocosms). The first trophic treatment was a “no-predator” configuration, composed of four snails and four 3-cm-diameter circular pieces of&nbsp;<em>Mazzaella</em>&nbsp;macroalgae cut out of blades (four pieces = 0.33&nbsp;g ± 0.03 in total, with each piece standardized to have similar initial mass), both placed on one side of the central barrier of the mesocosm. The second trophic treatment was a “cue only” treatment in which one sea star was housed on one side of the barrier with four snails and macroalgae on the other side. The third was a “complete interaction” treatment in which one sea star, four snails, and macroalgae were all located on the same side of the barrier. The final trophic treatment was a “no prey/no grazing” configuration, for which one sea star was placed on one side of the barrier with the macroalgae on the other. The final trophic treatment was a “no prey/no grazing” configuration, for which one sea star was placed on one side of the barrier with the macroalgae on the other. It was used primarily as a control for predator behavior in the absence of prey, and also provided a control for any changes in macroalgal mass that might occur without grazing, due, for example, to growth or senescence.</p>
<p>Consequences of pH for the strength of net top-down indirect effects were estimated from relative reductions in mass of macroalgae in the no-predator treatment, the cue-only treatment, and the complete interaction treatment across pH levels. Wet weight (blotted dry) of disks excised from&nbsp;<em>Mazzaella</em>&nbsp;blades was measured before and after the 7 days to assess macroalgal mass loss. First-order estimates of macroalgal consumption were calculated as the difference in macroalgal mass loss between each container of a trophic treatment and the group mean from the no-grazing treatment for each pH level (algae eaten).</p>
<p>See Jellison, B.M. &amp; Gaylord, B. <em>Oecologia</em> (2019).&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1636191 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1636191
completed
Brian Gaylord
University of California - Davis: Bodega Marine Laboratory
707-875-1940
Bodega Marine Laboratory & Department of Evolution and Ecology 2099 Westshore Road, P.O. Box 247
Bodega Bay
CA
94923
USA
bpgaylord@ucdavis.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Container
pH
Trophic_Treatment
Initial_Weight
End_Weight
Mass_loss
Algae_Eaten
Number_of_snails_end
Algae_Eaten_per_snail
Mettler AE 160 balance
theme
None, User defined
number
pH
treatment
weight_g
mass
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
scale or balance
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Trophic consequences of ocean acidification: Intertidal sea star predators and their grazer prey
https://osprey.bco-dmo.org/project/712799
Trophic consequences of ocean acidification: Intertidal sea star predators and their grazer prey
<p><em>NSF Award Abstract:</em><br />
The absorption of human-produced carbon dioxide into the world's oceans is altering the chemistry of seawater, including decreasing its pH. Such changes, collectively called "ocean acidification", are expected to influence numerous types of sea creatures. This project examines how shifts in ocean pH affect animal behavior and thus interactions among species. It uses a case study system that involves sea star predators, snail grazers that they eat, and seaweeds consumed by the latter. The rocky-shore habitats where these organisms live have a long history of attention, and new findings from this work will further extend an already-large body of marine ecological knowledge. The project provides support for graduate and undergraduate students, including underrepresented students from a nearby community college. The project underpins the development of a new educational module for local K-12 schools. Findings will moreover be communicated to the public through the use of short film documentaries, as well as through established relationships with policy, management, and industry groups, and contacts with the media.</p>
<p>Ocean acidification is a global-scale perturbation. Most research on the topic, however, has examined effects on single species operating in isolation, leaving interactions among species underexplored. This project confronts this knowledge gap by considering how ocean acidification may shift predator-prey relationships through altered behavior. It targets as a model system sea stars, their gastropod grazer prey, and macoalgae consumed by the latter, via four lines of inquiry. 1) The project examines the functional response of the focal taxa to altered seawater chemistry, using experiments that target up to 16 discrete levels of pH. This experimental design is essential for identifying nonlinearities and tipping points. 2) The project addresses both consumptive and non-consumptive components of direct and indirect species interactions. The capacity of ocean acidification to influence such links is poorly known, and better understanding of this issue is a recognized priority. 3) The project combines controlled laboratory experiments with field trials that exploit tide pools and their unique pH signatures as natural mesocosms. Field tests of ocean acidification effects are relatively rare and are sorely needed. 4) A final research phase expands upon the above three components to address effects of ocean acidification on multiple additional taxa that interact in rocky intertidal systems, to provide a broad database that may have utility for future experiments or modeling.</p>
BOAR Trophic
largerWorkCitation
project
eng; USA
oceans
-123.04805
-123.04805
38.33325
38.33325
2015-07-11
2015-07-21
Central California coast, USA
0
BCO-DMO catalogue of parameters from Mesocosm study of trophic interactions under ocean acidification, focusing on the consumption of algae by snails in Bodega Bay, California
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/870745.rdf
Name: Container
Units: unitless
Description: <p>Container number</p>
http://lod.bco-dmo.org/id/dataset-parameter/870746.rdf
Name: pH
Units: unitless
Description: <p>pH treatment level of the container; Low pH ~ 7.0 total pH, Ambient pH ~7.9 total pH</p>
http://lod.bco-dmo.org/id/dataset-parameter/870747.rdf
Name: Trophic_Treatment
Units: unitless
Description: <p>Trophic treatment condition; “no-predator” = four snails and four circular pieces of macroalgae on one side of the barrier, “cue only" = one sea star was housed on one side of the barrier with four snails and macroalgae on the other side, "complete interaction" = one sea star, four snails, and macroalgae all placed on one side of the barrier together, “no prey/no grazing” = one sea star was placed on one side of the barrier with the macroalgae on the other.</p>
http://lod.bco-dmo.org/id/dataset-parameter/870748.rdf
Name: Initial_Weight
Units: grams
Description: <p>wet weight (g) of all four macroalgae circular pieces weighed together for each container at the beginning of the experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/870749.rdf
Name: End_Weight
Units: grams
Description: <p>Wet weight in grams (g) of all four macroalgae circular pieces weighed together for each container at the end of the experiment.</p>
<p>Two blanks are present in which mesocosms were excluded from the macroalgal analysis, because the macroalgae fragmented during extraction, preventing assessment of the quantity consumed by the snails.</p>
http://lod.bco-dmo.org/id/dataset-parameter/870750.rdf
Name: Mass_loss
Units: grams
Description: <p>The difference in wet mass in grams (g) of algae at the beginning and end of the experiment for each container</p>
<p>Two blanks are present in which mesocosms were excluded from the macroalgal analysis, because the macroalgae fragmented during extraction, preventing assessment of the quantity consumed by the snails.</p>
http://lod.bco-dmo.org/id/dataset-parameter/870751.rdf
Name: Algae_Eaten
Units: grams
Description: <p>First-order estimates of macroalgal consumption calculated as the difference in the macroalgal mass loss in grams (g) between each container of a trophic treatment and the group mean from the no-grazing treatment for each pH level.</p>
<p>Blanks are present for the no-grazing treatments which did not contain grazers and in which estimates of macroalgal consumption were not calculated. Two additional blanks are present in which mesocosms were excluded from the macroalgal analysis, because the macroalgae fragmented during extraction, preventing assessment of the quantity consumed by the snails.</p>
http://lod.bco-dmo.org/id/dataset-parameter/870752.rdf
Name: Number_of_snails_end
Units: number
Description: <p>The number of snails remaining at the end of the experiment for each contianer. Each container that contained snails began with 4 snails each.</p>
http://lod.bco-dmo.org/id/dataset-parameter/870753.rdf
Name: Algae_Eaten_per_snail
Units: grams
Description: <p>First-order estimates of macroalgal consumption (Algae eaten) divided by the number of snails remaining in that container at the end of the experiment.</p>
<p>Blanks are present for the no-grazing treatments which did not contain grazers and in which estimates of macroalgal consumption were not calculated. Two additional blanks are present in which mesocosms were excluded from the macroalgal analysis, because the macroalgae fragmented during extraction, preventing assessment of the quantity consumed by the snails.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2759
https://darchive.mblwhoilibrary.org/bitstream/1912/28204/1/dataset-866359_mesocosm-study-algae-eaten__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.866359.1
download
onLine
dataset
<p>This dataset is part of a larger experiment to investigate how pH influences trophic links between intertidal sea stars (<em>Leptasterias hexactis</em>), snails (<em>Tegula funebralis</em>), and macroalgae (<em>Mazzaella flaccida)</em>. These data were gathered during an experiment at the Bodega Marine Laboratory. Wet weight (hand blotted; grams) of algae was determined before and after the experiment using an analytical balance (Mettler AE 160).</p>
<p>Organisms were placed for 7 days in mesocosms containing seawater at either ambient (~7.9) or low pH (~7.0). The pH was modified using equimolar additions of sodium bicarbonate (NaHCO3) and hydrochloric acid (HCl). The water in each container was changed daily. The mesocosm array consisted of 40, 13-liter (L) circular plastic containers with a mesh barrier down the center to separate predator, prey, and/or basal resource but allowing for passage of waterborne cue. Mesocosms were filled halfway with seawater, allowing 10 centimeters (cm) of refuge space for snails above the waterline. Mesocosms were held within a seawater table under constant flow to maintain consistent temperatures.</p>
<p>Each mesocosm was assigned to one of four trophic treatments and one of two pH levels, resulting in five replicates per treatment and pH (4 trophic × 2 pH × 5 replicates = 40 mesocosms). The first trophic treatment was a “no-predator” configuration, composed of four snails and four 3-cm-diameter circular pieces of&nbsp;<em>Mazzaella</em>&nbsp;macroalgae cut out of blades (four pieces = 0.33&nbsp;g ± 0.03 in total, with each piece standardized to have similar initial mass), both placed on one side of the central barrier of the mesocosm. The second trophic treatment was a “cue only” treatment in which one sea star was housed on one side of the barrier with four snails and macroalgae on the other side. The third was a “complete interaction” treatment in which one sea star, four snails, and macroalgae were all located on the same side of the barrier. The final trophic treatment was a “no prey/no grazing” configuration, for which one sea star was placed on one side of the barrier with the macroalgae on the other. The final trophic treatment was a “no prey/no grazing” configuration, for which one sea star was placed on one side of the barrier with the macroalgae on the other. It was used primarily as a control for predator behavior in the absence of prey, and also provided a control for any changes in macroalgal mass that might occur without grazing, due, for example, to growth or senescence.</p>
<p>Consequences of pH for the strength of net top-down indirect effects were estimated from relative reductions in mass of macroalgae in the no-predator treatment, the cue-only treatment, and the complete interaction treatment across pH levels. Wet weight (blotted dry) of disks excised from&nbsp;<em>Mazzaella</em>&nbsp;blades was measured before and after the 7 days to assess macroalgal mass loss. First-order estimates of macroalgal consumption were calculated as the difference in macroalgal mass loss between each container of a trophic treatment and the group mean from the no-grazing treatment for each pH level (algae eaten).</p>
<p>See Jellison, B.M. &amp; Gaylord, B. <em>Oecologia</em> (2019).&nbsp;</p>
Specified by the Principal Investigator(s)
<p>Algae eaten is calculated as the difference in macroalgal mass loss&nbsp; (g) between each container of a trophic treatment and the group mean from the no-grazing treatment for each pH level.</p>
<p>Algae eaten per snail is calculated as macroalgal consumption (Algae eaten) divided by the number of snails remaining in that container at the end of the experiment.</p>
<p><strong>BCO-DMO processing description:</strong><br />
- Adjusted field/parameter names to comply with BCO-DMO naming conventions<br />
- Replaced blank values with "nd" (no data)<br />
- Added a conventional header with dataset name, PI names, version date</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Mettler AE 160 balance
Mettler AE 160 balance
PI Supplied Instrument Name: Mettler AE 160 balance Instrument Name: scale or balance Instrument Short Name: Instrument Description: Devices that determine the mass or weight of a sample. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB13/