<div><p><strong>Experiment Overview:</strong><br />
The effect of strontium (Sr) on the coccolithophore <em>Scyphosphaera apsteinii</em> calcification was assessed over a 10 d period. This species of coccolithophore has unusually high levels of Sr in its calcite coccoliths as detected with Energy-dispersive X-ray spectroscopy (EDS), whereas Si levels are undetectable with EDS. The goal of the experiment was to determine whether Sr in seawater plays a significant role in coccolith production and/or coccolith crystal morphology. <em>S. apsteinii </em>was acclimated and grown in a range of Sr concentrations (deplete: 0.33 mmol/mol Sr/Ca, ambient: 9 mmol/mol Sr/Ca, and higher than ambient: 36 and 72 mmol/mol Sr/Ca). All treatments had four replicate flasks. Aliquots of cultures for cell counts and Fv/Fm were taken every two days between the start (T0 for cell counts, T4 for Fv/Fm) and end (T10) of the experiment. When cells were at mid-exponential phase (T4-T6) aliquots were collected for scanning electron microscopy (SEM) and EDS analysis to observe morphology and determine Sr incorporation into calcite coccoliths, respectively.</p>
<p>The laboratory experiments were conducted in March 2019 at the University of North Carolina - Wilmington. SEM/EDS analysis was done at the Analytical Instrumentation Facility at NC State from April 19th to May 9th, 2019.</p>
<p><strong>Cell Counting:</strong><br />
Cells were counted on each collection day using a hemocytometer or Sedgwick-Rafter chamber. A minimum of 300 cells were counted per sample. Growth curves were plotted and specific growth rates were calculated for each species throughout the 8 d sampling period.</p></div>
Sr Experiment: Cell Counts
Sr Experiment: Cell Counts
<div><p><strong>Data Processing:</strong><br />
Cell counts (cells per mL) were used to determine growth rate (r) and doublings per day (k) for each species using the following equations:<br />
Rate of increase (r) = [(LN avg cells t0) - (LN avg cells t1)] / (t1 - t0)</p>
<p>Doublings per day (k) = r / 0.6931</p>
<p>LN is the natural log of the average cell numbers per L. t is the timepoint. </p>
<p><strong>BCO-DMO Processing:</strong><br />
- removed units from data columns so values can be typed as numeric.</p></div>
866456
Sr Experiment: Cell Counts
2021-12-09T16:13:31-05:00
2021-12-09T16:13:31-05:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:866456
Cell counts from an experiment examining the effect of Sr on the coccolithophore Scyphosphaera apsteinii calcification
The effect of Sr on the coccolithophore Scyphosphaera apsteinii calcification was assessed over a 10 day period. Cells were counted on each collection day using a hemocytometer or Sedgwick-Rafter chamber.
false
Taylor, A. (2022) Cell counts from an experiment examining the effect of Sr on the coccolithophore Scyphosphaera apsteinii calcification. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-12-10 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.866456.1 [access date]
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1
10.26008/1912/bco-dmo.866456.1
false
2021-12-10
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http://lod.bco-dmo.org/id/dataset/866456
2019-03 - 2019-03
2019-03
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2019
2019-03
--03
2019