http://lod.bco-dmo.org/id/dataset/866774
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2021-12-14
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Bulk isotopic composition (d15N and d13C) of abyssal megafauna and macrofauna from Stations M and Aloha
2022-01-12
publication
2022-01-12
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2022-01-12
publication
https://doi.org/10.26008/1912/bco-dmo.866774.1
Jeffrey C. Drazen
University of Hawaiʻi at Mānoa
principalInvestigator
Brian N. Popp
University of Hawaiʻi at Mānoa
principalInvestigator
Craig R. Smith
University of Hawaiʻi at Mānoa
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Drazen, J. C., Popp, B. N., Smith, C. R. (2022) Bulk isotopic composition (d15N and d13C) of abyssal megafauna and macrofauna from Stations M and Aloha. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-01-12 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.866774.1 [access date]
Abyssal Bulk Isotope Analyses Dataset Description: Methods and Sampling: <p>Macrofauna and megafauna were collected in May and October 2019 using the HOV <em>Alvin </em>and the ROV <em>Doc Ricketts</em>, respectively, at Station M (34° 50'N, 123° 00'W) and in July 2019, January 2020, and July 2020 using ROV Lu'ukai at Station Aloha (22° 45'N, 158° 00'W). Macrofauna at Station M were collected using HOV-operated Ekman cores (20x20 cm). Macrofauna at Station Aloha were collected using a Brenke epibenthic sled towed across the seafloor (detailed in Glover et al 2016), due to the considerably lower macrofaunal densities at this oligotrophic site. Megafauna were collected using the submersible vehicle's manipulator arm and/or slurp gun. Upon retrieval to the surface, samples were placed in a cool room (5ºC) for their further processing. Specimens of megafauna were weighed and measured, then they were dissected using a scalpel. All tissue samples were placed in cryovials and frozen in liquid nitrogen, and subsequently stored at -80ºC. Sieved (300 um) macrofauna samples were preserved in 10% buffered formalin. In the laboratory, macrofauna were further sorted by taxon. Samples of megafauna body tissues or macrofauna were freeze dried and ground to a homogenous powder using mortar and pestle.</p>
<p>For analysis of bulk nitrogen and carbon isotopic composition, ~0.7 and 3 mg of body tissue from holothurians and echinoids, respectively, ~ 5 mg of gut content, and ~ 20 mg of sediments were placed in silver capsules. Samples were acidified to remove carbonates with 1M HCl, which was added dropwise until bubbling ceased, then dried at 60ºC and packed.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1829612 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1829612
completed
Jeffrey C. Drazen
University of Hawaiʻi at Mānoa
808-956-6567
Department of Oceanography, SOEST 1000 Pope Road
Honolulu
HI
96822
USA
jdrazen@hawaii.edu
pointOfContact
Brian N. Popp
University of Hawaiʻi at Mānoa
808-956-6206
Department of Earth Sciences 1680 East-West Road
Honolulu
HI
96822
USA
popp@hawaii.edu
pointOfContact
Craig R. Smith
University of Hawaiʻi at Mānoa
808-956-7776
Department of Oceanography 1000 Pope Road
Honolulu
HI
96822
USA
craigsmi@hawaii.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Sample_ID
Cruise
Station
Start_date
End_date
Taxon
animal_mass
tissue_type
Sample_weight
Acidified
lipid_extracted
ug_N
d15N_v_AIR
ug_C
d13C_PC_v_VPDB
Comments
Costech Model 4010
DeltaPlusXP or Delta-V-Advantage
Thermo Scientific Delta V Plus
Thermo-Fisher Scientific MAT 253
theme
None, User defined
sample identification
Cruise identfier
station
date
taxon
mass
sample type
weight
treatment
Nitrogen
d15N measured in biota
Elemental carbon (C)
d13C measured in biota
comments
featureType
BCO-DMO Standard Parameters
Costech International Elemental Combustion System (ECS) 4010
Isotope-ratio Mass Spectrometer
Isotope-ratio Mass Spectrometer
Isotope-ratio Mass Spectrometer
instrument
BCO-DMO Standard Instruments
KM2002
AT42-10
KM1914
KM2008
Pulse 72
service
Deployment Activity
Station Aloha
Eastern North Pacific 34.50° N 123.06° W (Station M)
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Assessing the relative importance of small vs large particles as sources of nutrition to abyssal communities
https://osprey.bco-dmo.org/project/775278
Collaborative Research: Assessing the relative importance of small vs large particles as sources of nutrition to abyssal communities
<p><em>NSF Award Abstract:</em><br />
The abyssal plains of the oceans cover roughly half of the earth's surface, host enormous reservoirs of biodiversity and mineral resources, and play important roles in nutrient recycling and carbon sequestration. The most important process controlling the structure and function of these ecosystems is the quantity and quality of food (mostly sinking organic particles) that reaches the deep-sea floor. However, we do not fully understand the processes provisioning this vast ecosystem. We propose to evaluate the relative importance of small and larger "marine snow" particles that sink to deep-sea benthic communities by using the stable isotope signature of amino acids within various food sources and trace their consumption by fauna on the seafloor. This project compares ecosystems from the productive waters off California with the nutrient poor central Pacific, north of Hawaii. This project provides novel insights into how surface ocean processes are coupled to food-webs at the deep ocean seafloor and how changes in food sources potentially impact deep-sea communities. This project also provides excellent training opportunities for graduate students, a postdoctoral researcher, and undergraduates at UH and USC, particularly underrepresented minorities who pursue majors in the geosciences. The project will sponsor an annual G6-12 teacher workshop to inform Hawaii educators about the deep sea and broadly disseminate knowledge to the community. All results are communicated broadly to inform the public as concerns regarding abyssal ecosystems are rising due to interests in deep-sea mining.</p>
<p>The most important process controlling the structure and function of abyssal ecosystems is the quantity and quality of organic material that ultimately reaches the deep-sea floor. Despite the strong relationship between euphotic zone export flux and benthic ecology, studies of abyssal ecosystems have observed a deficit between food supply and benthic community demand. Additional work is therefore needed, particularly with regards to understanding the sources of nutrition to the deep-sea benthos. Recent evidence suggests that small particles may be significant contributors to carbon export, increasing in relative importance with depth in the mesopelagic and reaching the abyssal seafloor. This project is to evaluate the relative importance of small and larger "marine snow" particles to deep-sea benthic communities using a combination of particle flux measurements and state of the art compound specific stable isotope analysis of amino acids (AA-CSIA) at two abyssal locations that contrast in overlying productivity, seasonality, and export magnitude. Time series measurements at these locations (Sta. M off California and Sta. Aloha off Hawaii) provide a rich context for the work. In the mesopelagic central North Pacific larger particles (>53 um) can be resolved from microbially reworked, smaller (0.7-53 um) particles using AA-CSIA. This project is characterizing the isotopic compositions of key individual compounds in a continuum of particle sizes (< 1.0 um suspended particles to large sinking particles >53 um) collected using in situ filtration near the seafloor and bottom-moored sediment traps, thereby defining source-specific isotopic signatures that can be traced into benthic fauna and sediments (that are collected by ROVs and epibenthic sleds). This research to understand pelagic-benthic coupling from particles to megafauna using isotopic measurements at the compound-level will yield novel insights into the importance of small microbially reworked particles to deep-sea benthic food webs. This will more precisely couple surface ocean processes to food-webs at the deep ocean seafloor with implications for understanding climate change effects and the efficiency of energy transfer to higher trophic levels. Furthermore, isotopic measurements can also be used to further parameterize ecosystem models by quantifying trophic position across size classes and thus estimate predator-prey mass ratios in relation to variation in body size spectra, functional type, and ultimately to carbon flux and remineralization. Finally, the results will help refine interpretations of deep-sea paleorecords of past nitrogen dynamics by calibrating potential changes in organic matter isotope values between the surface and seafloor archives.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
Abyssal food web
largerWorkCitation
project
eng; USA
oceans
Station Aloha; Eastern North Pacific 34.50° N 123.06° W (Station M)
-158
-123
22.75
34.833
2019-04-30
2020-08-02
California current, Station M (34º 50' N, 123º W) and North Pacific Subtropical Gyre, Station Aloha (22º 45' N, 158º W)
0
BCO-DMO catalogue of parameters from Bulk isotopic composition (d15N and d13C) of abyssal megafauna and macrofauna from Stations M and Aloha
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/866939.rdf
Name: Sample_ID
Units: unitless
Description: <p>Sample identification code</p>
http://lod.bco-dmo.org/id/dataset-parameter/866940.rdf
Name: Cruise
Units: unitless
Description: <p>Cruise designation</p>
http://lod.bco-dmo.org/id/dataset-parameter/866941.rdf
Name: Station
Units: unitless
Description: <p>Site designation: Station M or Station Aloha</p>
http://lod.bco-dmo.org/id/dataset-parameter/866942.rdf
Name: Start_date
Units: unitless
Description: <p>First date of collection or only date if sample was collected at a single point in time; format: MM-DD-YY</p>
http://lod.bco-dmo.org/id/dataset-parameter/866943.rdf
Name: End_date
Units: unitless
Description: <p>Last date of collection for pooled samples; format: YYYY-MM-DD</p>
http://lod.bco-dmo.org/id/dataset-parameter/866944.rdf
Name: Taxon
Units: unitless
Description: <p>Lowest taxon specimen was identified to</p>
http://lod.bco-dmo.org/id/dataset-parameter/866945.rdf
Name: animal_mass
Units: grams (g)
Description: <p>Mass of the animal</p>
http://lod.bco-dmo.org/id/dataset-parameter/866946.rdf
Name: tissue_type
Units: unitless
Description: <p>Type of sample: animal, gut content, muscle, liver, etc.</p>
http://lod.bco-dmo.org/id/dataset-parameter/866947.rdf
Name: Sample_weight
Units: milligrams (mg)
Description: <p>Weight of dry sample analyzed</p>
http://lod.bco-dmo.org/id/dataset-parameter/866948.rdf
Name: Acidified
Units: unitless
Description: <p>Whether sample was acidified prior to analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/866949.rdf
Name: lipid_extracted
Units: unitless
Description: <p>Whether sample was lipid extracted prior to analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/866950.rdf
Name: ug_N
Units: micrograms (ug)
Description: <p>Micrograms (ug) of Nitrogen in sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/866951.rdf
Name: d15N_v_AIR
Units: per mil (‰), vs AIR
Description: <p>Bulk nitrogen isotopic composition</p>
http://lod.bco-dmo.org/id/dataset-parameter/866952.rdf
Name: ug_C
Units: micrograms (ug)
Description: <p>Micrograms (ug) of Carbon in sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/866953.rdf
Name: d13C_PC_v_VPDB
Units: per mil (‰), vs VPDB
Description: <p>Bulk carbon isotopic composition</p>
http://lod.bco-dmo.org/id/dataset-parameter/866954.rdf
Name: Comments
Units: unitless
Description: <p>Comments</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
68377
https://darchive.mblwhoilibrary.org/bitstream/1912/27914/1/dataset-866774_abyssal-bulk-isotope-analyses__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.866774.1
download
onLine
dataset
<p>Macrofauna and megafauna were collected in May and October 2019 using the HOV <em>Alvin </em>and the ROV <em>Doc Ricketts</em>, respectively, at Station M (34° 50'N, 123° 00'W) and in July 2019, January 2020, and July 2020 using ROV Lu'ukai at Station Aloha (22° 45'N, 158° 00'W). Macrofauna at Station M were collected using HOV-operated Ekman cores (20x20 cm). Macrofauna at Station Aloha were collected using a Brenke epibenthic sled towed across the seafloor (detailed in Glover et al 2016), due to the considerably lower macrofaunal densities at this oligotrophic site. Megafauna were collected using the submersible vehicle's manipulator arm and/or slurp gun. Upon retrieval to the surface, samples were placed in a cool room (5ºC) for their further processing. Specimens of megafauna were weighed and measured, then they were dissected using a scalpel. All tissue samples were placed in cryovials and frozen in liquid nitrogen, and subsequently stored at -80ºC. Sieved (300 um) macrofauna samples were preserved in 10% buffered formalin. In the laboratory, macrofauna were further sorted by taxon. Samples of megafauna body tissues or macrofauna were freeze dried and ground to a homogenous powder using mortar and pestle.</p>
<p>For analysis of bulk nitrogen and carbon isotopic composition, ~0.7 and 3 mg of body tissue from holothurians and echinoids, respectively, ~ 5 mg of gut content, and ~ 20 mg of sediments were placed in silver capsules. Samples were acidified to remove carbonates with 1M HCl, which was added dropwise until bubbling ceased, then dried at 60ºC and packed.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing:</strong><br />
- replaced "NA" and "na" with "nd" (no data);<br />
- renamed fields to comply with BCO-DMO naming conventions;<br />
- changed format of end dates to YYYY-MM-DD;<br />
- replaced commas in the Comments and animal_mass fields with semi-colons;<br />
- replaced erroneous cruise IDs of KM2009-KM2076 with KM2008;<br />
- replaced erroneous date of 2019-04-31 with 2019-04-30.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Costech Model 4010
Costech Model 4010
PI Supplied Instrument Name: Costech Model 4010 PI Supplied Instrument Description:Bulk nitrogen and carbon isotopic composition were determined using an isotope ratio mass spectrometer (DeltaPlusXP or Delta-V-Advantage) coupled to an elemental analyzer (Costech Model 4010). Instrument Name: Costech International Elemental Combustion System (ECS) 4010 Instrument Short Name:Costech ECS 4010 Instrument Description: The ECS 4010 Nitrogen / Protein Analyzer is an elemental combustion analyser for CHNSO elemental analysis and Nitrogen / Protein determination. The GC oven and separation column have a temperature range of 30-110 degC, with control of +/- 0.1 degC.
DeltaPlusXP or Delta-V-Advantage
DeltaPlusXP or Delta-V-Advantage
PI Supplied Instrument Name: DeltaPlusXP or Delta-V-Advantage PI Supplied Instrument Description:Bulk nitrogen and carbon isotopic composition were determined using an isotope ratio mass spectrometer (DeltaPlusXP or Delta-V-Advantage) coupled to an elemental analyzer (Costech Model 4010). Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Thermo Scientific Delta V Plus
Thermo Scientific Delta V Plus
PI Supplied Instrument Name: Thermo Scientific Delta V Plus PI Supplied Instrument Description:Nitrogen isotope composition of amino acids was determined using an isotope ratio mass spectrometer (Thermo Scientific Delta V Plus) interfaced with a Thermo Finnigan GC-C III. Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Thermo-Fisher Scientific MAT 253
Thermo-Fisher Scientific MAT 253
PI Supplied Instrument Name: Thermo-Fisher Scientific MAT 253 PI Supplied Instrument Description:Carbon isotope composition of amino acids was measured using a Thermo-Fisher Scientific MAT 253 isotope ratio mass spectrometer interfaced with a Trace Ultra GC-III via ConFlo IV. Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Cruise: KM2002
KM2002
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel
KM2002
Jeffrey C. Drazen
University of Hawaiʻi at Mānoa
Cruise: AT42-10
AT42-10
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
AT42-10
Jeffrey C. Drazen
University of Hawaiʻi at Mānoa
Cruise: KM1914
KM1914
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel
KM1914
Jeffrey C. Drazen
University of Hawaiʻi at Mānoa
Cruise: KM2008
KM2008
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel
KM2008
Jeffrey C. Drazen
University of Hawaiʻi at Mānoa
Cruise: Pulse 72
Pulse 72
R/V Western Flyer
International Council for the Exploration of the Sea
R/V Western Flyer
vessel
Pulse 72
Kenneth Smith
Monterey Bay Aquarium Research Institute
R/V Kilo Moana
Community Standard Description
International Council for the Exploration of the Sea
R/V Kilo Moana
vessel
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
R/V Western Flyer
International Council for the Exploration of the Sea
R/V Western Flyer
vessel