http://lod.bco-dmo.org/id/dataset/866781
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2021-12-15
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Nutrient concentrations and cell/virus-like particles counts
2022-09-21
publication
2022-09-21
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2022-09-21
publication
https://doi.org/10.26008/1912/bco-dmo.866781.1
Jed A. Fuhrman
University of Southern California
principalInvestigator
Ella Sieradzki
University of Southern California
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Fuhrman, J. A., Sieradzki, E. (2022) Nutrient concentrations and cell/virus-like particles counts. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-09-21 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.866781.1 [access date]
nutrient concentrations & particle counts Dataset Description: <p>Related sequence data for the sampling locations can be found here:</p>
<p>* The project accession number for raw data on NCBI is PRJEB12234<br />
* The project accession number for partial genomes of viruses is PRJNA472807</p> Methods and Sampling: <p>Whole surface seawater for nutrients measurement were collected in triplicates in 50 mL conical tubes and kept in −20° C until flow-injection analysis at the Marine Sciences Institute Analytical Lab at University of California, Santa Barbara (http://www.msi.ucsb.edu/services/analytical-lab). Water collected by bucket.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1737409 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1737409
completed
Jed A. Fuhrman
University of Southern California
213-740-5757
Dept. Biological Sciences, Dornsife College of Letters, Arts, and Sciences 3616 Trousdale Parkway
Los Angeles
CA
90089-0371
USA
fuhrman@usc.edu
pointOfContact
Ella Sieradzki
University of Southern California
ella.shir@gmail.com
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Date
Sample_ID
Latitude
Longitude
Phosphate
Phosphate_flag
Silicate
Silicate_flag
Nitrite_Nitrate
Nitrite_Nitrate_flag
Chl_a
Bacteria_archaea
Bacteria_archaea_stdev
Viruses
Viruses_stdev
theme
None, User defined
date
sample identification
latitude
longitude
phosphate
flag
Silicate, Si(OH)4, silicic acid
nitrate plus nitrite
chlorophyll a
count
standard deviation
featureType
BCO-DMO Standard Parameters
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Protistan, prokaryotic, and viral processes at the San Pedro Ocean Time-series
https://osprey.bco-dmo.org/project/743049
Protistan, prokaryotic, and viral processes at the San Pedro Ocean Time-series
<p>Planktonic marine microbial communities consist of a diverse collection of bacteria, archaea, viruses, protists (phytoplankton and protozoa) and small animals (metazoan). Collectively, these species are responsible for virtually all marine pelagic primary production where they form the basis of food webs and carry out a large fraction of respiratory processes. Microbial interactions include the traditional role of predation, but recent research recognizes the importance of parasitism, symbiosis and viral infection. Characterizing the response of pelagic microbial communities and processes to environmental influences is fundamental to understanding and modeling carbon flow and energy utilization in the ocean, but very few studies have attempted to study all of these assemblages in the same study. This project is comprised of long-term (monthly) and short-term (daily) sampling at the San Pedro Ocean Time-series (SPOT) site. Analysis of the resulting datasets investigates co-occurrence patterns of microbial taxa (e.g. protist-virus and protist-prokaryote interactions, both positive and negative) indicating which species consistently co-occur and potentially interact, followed by examination gene expression to help define the underlying mechanisms. This study augments 20 years of baseline studies of microbial abundance, diversity, rates at the site, and will enable detection of low-frequency changes in composition and potential ecological interactions among microbes, and their responses to changing environmental forcing factors. These responses have important consequences for higher trophic levels and ocean-atmosphere feedbacks. The broader impacts of this project include training graduate and undergraduate students, providing local high school student with summer lab experiences, and PI presentations at local K-12 schools, museums, aquaria and informal learning centers in the region. Additionally, the PIs advise at the local, county and state level regarding coastal marine water quality.</p>
<p>This research project is unique in that it is a holistic study (including all microbes from viruses to small metazoa) of microbial species diversity and ecological activities, carried out at the SPOT site off the coast of southern California. In studying all microbes simultaneously, this work aims to identify important ecological interactions among microbial species, and identify the basis(es) for those interactions. This research involves (1) extensive analyses of prokaryote (archaean and bacterial) and eukaryote (protistan and micro-metazoan) diversity via the sequencing of marker genes, (2) studies of whole-community gene expression by eukaryotes and prokaryotes in order to identify key functional characteristics of microorganismal groups and the detection of active viral infections, and (3) metagenomic analysis of viruses and bacteria to aid interpretation of transcriptomic analyses using genome-encoded information. The project includes exploratory metatranscriptomic analysis of poorly-understood aphotic and hypoxic-zone protists, to examine their stratification, functions and hypothesized prokaryotic symbioses.</p>
SPOT
largerWorkCitation
project
eng; USA
oceans
2012-07-01
2013-04-30
San Pedro Channel off the coast of Los Angeles
0
BCO-DMO catalogue of parameters from Nutrient concentrations and cell/virus-like particles counts
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/880779.rdf
Name: Date
Units: unitless
Description: <p>Sample collection date</p>
http://lod.bco-dmo.org/id/dataset-parameter/880780.rdf
Name: Sample_ID
Units: unitless
Description: <p>Sample identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/880781.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Sample collection longitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/880782.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Sample collection latitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/880783.rdf
Name: Phosphate
Units: micromolar (µM)
Description: <p>concentration of phosphate</p>
http://lod.bco-dmo.org/id/dataset-parameter/880784.rdf
Name: Phosphate_flag
Units: unitless
Description: <p>below detection</p>
http://lod.bco-dmo.org/id/dataset-parameter/880785.rdf
Name: Silicate
Units: micromolar (µM)
Description: <p>concentration of silicate</p>
http://lod.bco-dmo.org/id/dataset-parameter/880786.rdf
Name: Silicate_flag
Units: unitless
Description: <p>below detection</p>
http://lod.bco-dmo.org/id/dataset-parameter/880787.rdf
Name: Nitrite_Nitrate
Units: micromolar (µM)
Description: <p>concentration of nitrite/nitrate</p>
http://lod.bco-dmo.org/id/dataset-parameter/880788.rdf
Name: Nitrite_Nitrate_flag
Units: unitless
Description: <p>below detection</p>
http://lod.bco-dmo.org/id/dataset-parameter/880789.rdf
Name: Chl_a
Units: migrograms per liter (µg/L)
Description: <p>concentration of chlorophyll A</p>
http://lod.bco-dmo.org/id/dataset-parameter/880790.rdf
Name: Bacteria_archaea
Units: cells per ml
Description: <p>number of cells per ml seawater measured by epifluroscent microscopy using SYBR gold</p>
http://lod.bco-dmo.org/id/dataset-parameter/880791.rdf
Name: Bacteria_archaea_stdev
Units: cells per ml
Description: <p>number of cells per ml seawater measured by epifluroscent microscopy using SYBR gold</p>
http://lod.bco-dmo.org/id/dataset-parameter/880792.rdf
Name: Viruses
Units: viruses per ml
Description: <p>number of virus like particles per ml seawater measured by epifluroscent microscopy using SYBR gold</p>
http://lod.bco-dmo.org/id/dataset-parameter/880793.rdf
Name: Viruses_stdev
Units: viruses per ml
Description: <p>number of virus like particles per ml seawater measured by epifluroscent microscopy using SYBR gold</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
1299
https://darchive.mblwhoilibrary.org/bitstream/1912/29365/1/dataset-866781_nutrient-concentrations-and-cellvirus-particles-counts__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.866781.1
download
onLine
dataset
<p>Whole surface seawater for nutrients measurement were collected in triplicates in 50 mL conical tubes and kept in −20° C until flow-injection analysis at the Marine Sciences Institute Analytical Lab at University of California, Santa Barbara (http://www.msi.ucsb.edu/services/analytical-lab). Water collected by bucket.</p>
Specified by the Principal Investigator(s)
<p>Bacteria and viruses per ml seawater were counted on duplicate slides using SYBR green epifluorescence microscopy.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact