http://lod.bco-dmo.org/id/dataset/868493
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2022-01-19
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Fecundity assessment of Acropora cervicornis colonies from spawning observations and gamete bundle analysis in August 2020 at Mote Marine Laboratory
2022-03-15
publication
2022-03-15
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2022-03-24
publication
https://doi.org/10.26008/1912/bco-dmo.868493.1
Erinn M. Muller
Mote Marine Laboratory
principalInvestigator
Hanna Koch
Mote Marine Laboratory
principalInvestigator
Erich Bartels
Mote Marine Laboratory
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Koch, H., Azu, Y., Bartels, E., Muller, E. (2022) Fecundity assessment of Acropora cervicornis colonies from spawning observations and gamete bundle analysis in August 2020 at Mote Marine Laboratory. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-03-15 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.868493.1 [access date]
Dataset Description: <p>This dataset is part of a larger study of&nbsp;<em>Acropora cervicornis </em>(staghorn) corals studied at Mote Marine Lab’s Elizabeth Moore International Center for Coral Reef Research and Restoration.&nbsp; The different analyses are listed here, and links to other data from this study can be found in the Related Datasets section below.&nbsp;&nbsp;</p>
<p>Analyses undertaken include:</p>
<ol>
<li>Total Population (Colony size dataset)</li>
<li>Morphometric Assessment (Colony size dataset)</li>
<li>Primary&nbsp;Fecundity Analysis (Population subset of Colony size dataset, plus Polyps dataset)</li>
<li>Dissections (Oocyte number dataset, Oocyte size dataset)</li>
<li>Secondary Fecundity Analysis (<strong>this dataset of Gamete Bundles</strong>)</li>
</ol>
<p>See Related Datasets section below for links to above mentioned datasets.</p> Methods and Sampling: <p>Sampling of <em>Acropora cervicornis</em> coral genotypes took place at Mote Marine Lab's spawning nurseries at Sand Key (24.45782, -81.88595) and Looe Key (24.56257, -81.40009).&nbsp;<br />
<br />
Sampling for Primary Fecundity Analysis was performed on July 3, 2020.&nbsp; From 5 colonies of every genet, 3 linear branches (~10 cm in length) were sampled using bone cutters from the central portion of each colony (see Colony Size dataset,&nbsp;https://www.bco-dmo.org/dataset/843028). Using a ruler, the number of polyps per one square centimeter was recorded from every branch near the base of the fragment (see Polyps per Area dataset,<a href="https://www.bco-dmo.org/dataset/868308">https://www.bco-dmo.org/dataset/868308</a>).</p>
<p>Treatments with formalin, DI, and HCl solution were done&nbsp;to preserve and decalcify the coral, which were then stored until dissection (see datasets <span style="font-family:arial,sans-serif; font-size:10pt">Oocyte Size, </span><a href="https://www.bco-dmo.org/dataset/843067">https://www.bco-dmo.org/dataset/843067</a><span style="font-family:arial,sans-serif; font-size:10pt"> and Oocyte Number, </span>&nbsp;<a href="https://www.bco-dmo.org/dataset/867314">https://www.bco-dmo.org/dataset/867314</a><span style="font-family:arial,sans-serif; font-size:10pt">).</span></p>
<p><strong>Sampling for Secondary Fecundity Analysis (this dataset)</strong><br />
As a secondary assessment of fecundity, five replicate colonies of every genet were brought back to the lab on July 31, 2020 for spawning and ex situ assisted sexual reproduction. From every genet that spawned during August 4-10, 2020 (all of them),&nbsp;40 random gamete bundles were collected during spawning using a transfer pipet and 50 mL falcon tube. Each gamete bundle was immediately placed into a 2 mL glass vial with 10% formalin for fixation and inverted several times to break up the bundle in each vial. Then, the total number of oocytes and sperm per bundle were counted. Eggs were counted by eye by two&nbsp;independent observers. Replicate sperm counts were recorded using a hemocytometer.&nbsp;&nbsp;</p>
<p><strong>Problem Report:</strong><br />
Genet 31: In the time between when the parental colonies were morphometrically assessed/sampled in the Sand Key nursery for the primary fecundity analysis and brought into the lab for spawning/sampled for the secondary fecundity analysis, the entire tree for genet 31 snapped off from its anchor and went missing. As such, 5 colonies of genet 31 were brought in from a different spawning nursery and location (Looe Key Nursery: 24.56257, -81.40009). Thus, the fecundity data obtained from the fragments and gamete bundles come from different subpopulations (fragments from Sand Key and gamete bundles from Looe Key).</p>
<p>Genet 41:&nbsp; From the primary fecundity analysis, it was determined that none of the colonies of genet 41 in the Sand Key nursery were gravid and therefore genet 41 was not included in the secondary fecundity analysis (Gamete Bundles Dataset).</p>
<p>Genets 62 + 63: Due to space limitations during transport of colonies from the field nursery to the lab, only 1 colony of genet 62 was brought in for assisted sexual reproduction and secondary fecundity analysis (Gamete Bundles Dataset).</p>
<div>
<div>
<div id="_com_1">
<p>&nbsp;</p>
</div>
</div>
</div>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1452538 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1452538
completed
Erinn M. Muller
Mote Marine Laboratory
941-388-4441 x 310
1600 Ken Thompson Parkway
Sarasota
FL
34241
USA
emuller@mote.org
pointOfContact
Hanna Koch
Mote Marine Laboratory
305-745-2425
24244 Overseas Highway
Summerland Key, FL
FL
33042
United States
hkoch@mote.org
pointOfContact
Erich Bartels
Mote Marine Laboratory
305-745-2425
24244 Overseas Highway
Summerland Key, FL
FL
33042
United States
ebartels@mote.org
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Location
Latitude
Longitude
Date_start_collect
Date_end_collect
Genotype
Phenotype
Replicate_Gamete_Bundle
Total_Sperm_per_bundle_in_millions
Total_Eggs_per_bundle
Calcutta metal pliers
Neubauer hemocytometer
AmScope compound microscope with ocular micrometer
articulating dissecting microscope
theme
None, User defined
site
latitude
longitude
date
sample type
sample identification
count
featureType
BCO-DMO Standard Parameters
bone cutter
Hemocytometer
Microscope - Optical
Microscope - Optical
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
CAREER: Applying phenotypic variability to identify resilient Acropora cervicornis genotypes in the Florida Keys
https://osprey.bco-dmo.org/project/642850
CAREER: Applying phenotypic variability to identify resilient Acropora cervicornis genotypes in the Florida Keys
<p><em>NSF Award Abstract:</em><br />
Caribbean staghorn coral was one of the most common corals within reefs of the Florida Keys several decades ago. Over the last 40 years disease, bleaching, overfishing and habitat degradation caused a 95% reduction of the population. Staghorn coral is now listed as threatened under the U.S. Endangered Species Act of 1973. Within the past few years, millions of dollars have been invested for the purpose of restoring the population of staghorn coral within Florida and the U.S. Virgin Islands. Significant effort has been placed on maintaining and propagating corals of known genotypes within coral nurseries for the purpose of outplanting. However, little is known about the individual genotypes that are currently being outplanted from nurseries onto coral reefs. Are the genotypes being used for outplanting resilient enough to survive the three major stressors affecting the population in the Florida Keys: disease, high water temperatures, and ocean acidification? The research within the present study will be the first step in answering this critically important question. The funded project will additionally develop a research-based afterschool program with K-12 students in the Florida Keys and U.S. Virgin Islands that emphasizes an inquiry-based curriculum, STEM research activities, and peer-to-peer mentoring. The information from the present study will help scientists predict the likelihood of species persistence within the lower Florida Keys under future climate-change and ocean-acidification scenarios. Results of this research will also help guide restoration efforts throughout Florida and the Caribbean, and lead to more informative, science-based restoration activities.</p>
<p><em>Acropora cervicornis</em> dominated shallow-water reefs within the Florida Keys for at least the last half a million years, but the population has recently declined due to multiple stressors. Understanding the current population level of resilience to three major threats - disease outbreaks, high water temperatures, and ocean acidification conditions - is critical for the preservation of this threatened species. Results from the present study will answer the primary research question: will representative genotypes from the lower Florida Keys provide enough phenotypic variation for this threatened species to survive in the future? The present proposal will couple controlled laboratory challenge experiments with field data and modeling applications, and collaborate with local educators to fulfill five objectives: 1) identify <em>A. cervicornis</em> genotypes resistant to disease, 2) identify <em>A. cervicornis</em> genotypes resilient to high water temperature and ocean acidification conditions, 3) quantify how high water temperature and ocean acidification conditions impact disease dynamics on <em>A. cervicornis</em>; 4) determine tradeoffs in life-history traits because of resilience factors; and 5) apply a trait-based model, which will predict genotypic structure of a population under different environmental scenarios.</p>
Resilient Acerv
largerWorkCitation
project
eng; USA
biota
oceans
-81.88814
-81.40009
24.45782
24.56257
2020-07-31
2020-08-10
Florida Keys, Summerland Key, FL 24.563595°, -81.278572°
0
BCO-DMO catalogue of parameters from Fecundity assessment of Acropora cervicornis colonies from spawning observations and gamete bundle analysis in August 2020 at Mote Marine Laboratory
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/871579.rdf
Name: Location
Units: unitless
Description: <p>Location of coral sampling site</p>
http://lod.bco-dmo.org/id/dataset-parameter/871580.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitude of spawning nursery</p>
http://lod.bco-dmo.org/id/dataset-parameter/871581.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitude of spawning nursery</p>
http://lod.bco-dmo.org/id/dataset-parameter/871582.rdf
Name: Date_start_collect
Units: unitless
Description: <p>Start date of spawning</p>
http://lod.bco-dmo.org/id/dataset-parameter/871583.rdf
Name: Date_end_collect
Units: unitless
Description: <p>End date of spawning</p>
http://lod.bco-dmo.org/id/dataset-parameter/871584.rdf
Name: Genotype
Units: unitless
Description: <p>Mote Marine Lab genet that was sampled (1, 3, 7, 13, 31, 34, 44, 47, 50, 62)</p>
http://lod.bco-dmo.org/id/dataset-parameter/871585.rdf
Name: Phenotype
Units: unitless
Description: <p>Phenotype that was sampled (S = white band disease susceptible, R = white band disease resistant)</p>
http://lod.bco-dmo.org/id/dataset-parameter/871586.rdf
Name: Replicate_Gamete_Bundle
Units: unitless
Description: <p>Which of the 40 gamete bundles was analyzed and counted</p>
http://lod.bco-dmo.org/id/dataset-parameter/871587.rdf
Name: Total_Sperm_per_bundle_in_millions
Units: unitless
Description: <p>Total number (in millions) of sperm counted per gamete bundle</p>
http://lod.bco-dmo.org/id/dataset-parameter/871588.rdf
Name: Total_Eggs_per_bundle
Units: unitless
Description: <p>Total number of eggs counted per gamete bundle</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
71779
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/4784b23e-077b-518d-86ed-f04c727fa506/content
download
https://doi.org/10.26008/1912/bco-dmo.868493.1
download
onLine
dataset
<p>Sampling of <em>Acropora cervicornis</em> coral genotypes took place at Mote Marine Lab's spawning nurseries at Sand Key (24.45782, -81.88595) and Looe Key (24.56257, -81.40009).&nbsp;<br />
<br />
Sampling for Primary Fecundity Analysis was performed on July 3, 2020.&nbsp; From 5 colonies of every genet, 3 linear branches (~10 cm in length) were sampled using bone cutters from the central portion of each colony (see Colony Size dataset,&nbsp;https://www.bco-dmo.org/dataset/843028). Using a ruler, the number of polyps per one square centimeter was recorded from every branch near the base of the fragment (see Polyps per Area dataset,<a href="https://www.bco-dmo.org/dataset/868308">https://www.bco-dmo.org/dataset/868308</a>).</p>
<p>Treatments with formalin, DI, and HCl solution were done&nbsp;to preserve and decalcify the coral, which were then stored until dissection (see datasets <span style="font-family:arial,sans-serif; font-size:10pt">Oocyte Size, </span><a href="https://www.bco-dmo.org/dataset/843067">https://www.bco-dmo.org/dataset/843067</a><span style="font-family:arial,sans-serif; font-size:10pt"> and Oocyte Number, </span>&nbsp;<a href="https://www.bco-dmo.org/dataset/867314">https://www.bco-dmo.org/dataset/867314</a><span style="font-family:arial,sans-serif; font-size:10pt">).</span></p>
<p><strong>Sampling for Secondary Fecundity Analysis (this dataset)</strong><br />
As a secondary assessment of fecundity, five replicate colonies of every genet were brought back to the lab on July 31, 2020 for spawning and ex situ assisted sexual reproduction. From every genet that spawned during August 4-10, 2020 (all of them),&nbsp;40 random gamete bundles were collected during spawning using a transfer pipet and 50 mL falcon tube. Each gamete bundle was immediately placed into a 2 mL glass vial with 10% formalin for fixation and inverted several times to break up the bundle in each vial. Then, the total number of oocytes and sperm per bundle were counted. Eggs were counted by eye by two&nbsp;independent observers. Replicate sperm counts were recorded using a hemocytometer.&nbsp;&nbsp;</p>
<p><strong>Problem Report:</strong><br />
Genet 31: In the time between when the parental colonies were morphometrically assessed/sampled in the Sand Key nursery for the primary fecundity analysis and brought into the lab for spawning/sampled for the secondary fecundity analysis, the entire tree for genet 31 snapped off from its anchor and went missing. As such, 5 colonies of genet 31 were brought in from a different spawning nursery and location (Looe Key Nursery: 24.56257, -81.40009). Thus, the fecundity data obtained from the fragments and gamete bundles come from different subpopulations (fragments from Sand Key and gamete bundles from Looe Key).</p>
<p>Genet 41:&nbsp; From the primary fecundity analysis, it was determined that none of the colonies of genet 41 in the Sand Key nursery were gravid and therefore genet 41 was not included in the secondary fecundity analysis (Gamete Bundles Dataset).</p>
<p>Genets 62 + 63: Due to space limitations during transport of colonies from the field nursery to the lab, only 1 colony of genet 62 was brought in for assisted sexual reproduction and secondary fecundity analysis (Gamete Bundles Dataset).</p>
<div>
<div>
<div id="_com_1">
<p>&nbsp;</p>
</div>
</div>
</div>
Specified by the Principal Investigator(s)
<p><u>Data processing:</u><br />
Data were compiled into Excel (Microsoft Office) and analyzed using R version 4.0.3 (2020-10-10) -- "Bunny-Wunnies Freak Out". Nonparametric statistical and correlation analyses were conducted.</p>
<p><u>BCO-DMO Processing:</u><br />
- separated Latitude and Longitude into separate columns<br />
-&nbsp;added conventional header with dataset name, PI name, version date<br />
-&nbsp;modified parameter names to conform with BCO-DMO naming conventions<br />
&nbsp;</p>
<p>&nbsp;</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Calcutta metal pliers
Calcutta metal pliers
PI Supplied Instrument Name: Calcutta metal pliers PI Supplied Instrument Description:Each ramet was cut from the donor colony using metal pliers (Calcutta bone cutters). Instrument Name: bone cutter Instrument Short Name: Instrument Description: A bone cutter is a surgical instrument used to cut bones or coral fragments.
Neubauer hemocytometer
Neubauer hemocytometer
PI Supplied Instrument Name: Neubauer hemocytometer PI Supplied Instrument Description:Replicate sperm counts were recorded using a hemocytometer Instrument Name: Hemocytometer Instrument Short Name:Hemocytometer Instrument Description: A hemocytometer is a small glass chamber, resembling a thick microscope slide, used for determining the number of cells per unit volume of a suspension. Originally used for performing blood cell counts, a hemocytometer can be used to count a variety of cell types in the laboratory. Also spelled as "haemocytometer". Description from:
http://hlsweb.dmu.ac.uk/ahs/elearning/RITA/Haem1/Haem1.html.
AmScope compound microscope with ocular micrometer
AmScope compound microscope with ocular micrometer
PI Supplied Instrument Name: AmScope compound microscope with ocular micrometer PI Supplied Instrument Description:Eggs were counted by eye by 2 independent observers Instrument Name: Microscope - Optical Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope". Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/
articulating dissecting microscope
articulating dissecting microscope
PI Supplied Instrument Name: articulating dissecting microscope PI Supplied Instrument Description:Eggs were counted by eye by 2 independent observers Instrument Name: Microscope - Optical Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope". Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/