http://lod.bco-dmo.org/id/dataset/868879
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2022-01-26
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
CTD data from R/V Hugh R. Sharp cruise HRS2110 in the Chesapeake Bay during August 2021
2022-01-26
publication
2022-01-26
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2022-02-09
publication
https://doi.org/10.26008/1912/bco-dmo.868879.1
Bess B. Ward
Princeton University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Ward, B. B. (2022) Cast data from cruise R/V Hugh R. Sharp HRS2110 in the Chesapeake Bay from August 2021 (NO2Ox_OMZs project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-01-26 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.868879.1 [access date]
Chesapeake Bay CTD 2021 Dataset Description: Methods and Sampling: <p>Water samples were collected using a 12 x 10-liter&nbsp;Niskin bottle rosette sampler equipped with a conductivity, temperature, and pressure instrument package (SBE9, Sea-Bird Electronics, Bellevue, Washington, U.S.A.), a sensor for dissolved oxygen (SBE43, Sea-Bird), and a sensor for chlorophyll fluorescence (FluoroWetlabECO, AFL FL Sensor).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1946516 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1946516
completed
Bess B. Ward
Princeton University
609-258-5150
Geosciences Department 217 Guyot Hall
Princeton
NJ
08544-1003
USA
bbw@princeton.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Cast
Station
ISO_DateTime_UTC
Date
time_UTC
timeS
depSM
prDM
t090C
t190C
c0S_per_m
c1S_per_m
sal00_1
sal11_1
timeQ
timeY
latitude
longitude
svCM_1
flECO_AFL
sbeox0ML_per_L
sbox0Mm_per_Kg
sbeox0Mg_per_L
sigma_E00
sigma_E11
potemp090C
potemp190C
svCM_2
sal00_2
sal11_2
flag
CTD Sea-Bird 9
Niskin bottle rosette sampler
SBE43, Sea-Bird
FluoroWetlabECO, AFL FL Sensor
theme
None, User defined
cast
station
ISO_DateTime_UTC
date
time of day
time_elapsed
depth
water pressure
water temperature at measurement depth
conductivity
salinity
latitude
longitude
sound velocity
fluorescence
dissolved Oxygen
density
potential temperature
flag
featureType
BCO-DMO Standard Parameters
CTD Sea-Bird 9
Niskin bottle
Sea-Bird SBE 43 Dissolved Oxygen Sensor
Wet Labs ECO-AFL/FL Fluorometer
instrument
BCO-DMO Standard Instruments
HRS2110
service
Deployment Activity
Chesapeake Bay
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Nitrite Oxidation in Oxygen Minimum Zones
https://osprey.bco-dmo.org/project/831333
Nitrite Oxidation in Oxygen Minimum Zones
<p><em>NSF Award Abstract:</em><br />
This research is grounded in the fundamental role of nitrogen in limiting production in the ocean. Nitrite is a pivotal compound in the nitrogen cycle: it can be oxidized to nitrate, and thus retained as an available nutrient, or it can be reduced to dinitrogen gas, and thus lost from the bioavailable nitrogen pool. Oxidation of nitrite by nitrite oxidizing bacteria (NOB) is the only biological pathway by which nitrate is produced, and all known NOB require oxygen for life. The reduction pathway is also carried out by microbes, in this case, bacteria that thrive only in the absence of oxygen. In previous experiments, however, both oxidation and reduction of nitrite were detected in the same samples from ocean waters in the absence of oxygen. We will investigate three explanations for the apparent oxidation of nitrite in the absence of oxygen on a research cruise to the low oxygen waters off the coast of Peru: 1) The presence of unknown kinds of NOB that do not require oxygen; 2) a new reaction called dismutation, which is possible but never detected in nature; 3) an artifact associated with oxygen stress in NOB. This research could lead to discovery of novel mechanisms and or novel organisms that determine the fate of nitrite and the availability of nitrogen to support primary production in the long run. This project will advance discovery and understanding while promoting teaching, training and learning by providing opportunities for Princeton students to get involved in and have hands on experience in research in the lab and potentially at sea. Both undergraduate and graduate students will participate in the research through internships and field experiences. We will also integrate our work at sea into teaching in the classroom via videos and assignments based on data collected during the cruise.</p>
<p>Nitrite oxidation is the only known biological process that produces nitrate, which comprises the largest fixed nitrogen reservoir in the ocean. Nitrite oxidation is carried out by nitrite oxidizing bacteria (NOB), and all known species are obligate aerobes. Nitrite reduction to N2 occurs in multiple microbial pathways, generally under anoxic conditions. Despite their apparent incompatibility regarding oxygen, both processes are detected in the low oxygen or anoxic waters of oxygen minimum zones (OMZs). Thus, the fate of nitrite in OMZs has implications for the global fixed N budget. Nitrite oxidation is detected at high rates in essentially zero oxygen water in the most oxygen depleted depth intervals in OMZ regions, which suggests that some nitrite oxidizers might possess anaerobic metabolic capabilities. Nitrite disproportionation (or dismutation), in which nitrite is simultaneously oxidized to nitrate and reduced to N2, is a thermodynamically favorable reaction, which would link the two processes in one organism – but it has never been observed in nature. The research proposed here will address two big questions about nitrite in the ocean: 1) How does anaerobic nitrite oxidation work? 2) What determines the fate of nitrite? The experimental approach will investigate three possible explanations for anaerobic nitrite oxidation: 1) Nitrite is oxidized to nitrate by different clades of NOB, which exhibit different tolerances/requirements for oxygen; 2) Nitrite dismutation, also performed by NOB, partially explains the cooccurrence of oxidation and reduction of nitrite; 3) Apparently anaerobic nitrite oxidation is indeed biologically mediated but does not always represent net production of nitrate from nitrite; rather it results from isotopic equilibration during enzyme-catalyzed interconversion of nitrite and nitrate. These questions will be addressed by performing a suite of 15N-tracer incubations at stations located within and outside of one of the major OMZs in the ocean, the Eastern Tropical South Pacific. The dependence of the rate processes on oxygen concentrations will be determined, and the composition of the microbial assemblages will be assessed in order to determine whether different microbial components are involved under different environmental conditions. The expression of genes involved in oxidation/reduction/ respiratory metabolisms at low oxygen concentrations will be measured across oxygen gradients and in oxygen manipulations to identify their potential role in supporting “anaerobic” nitrite oxidation. The possibility that the apparently anaerobic nitrite oxidation is due to an enzyme level interconversion between nitrite and nitrate, which does not lead to net nitrate production and is not linked to growth of nitrite oxidizing bacteria, will also be investigated.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
NO2Ox_OMZs
largerWorkCitation
project
eng; USA
oceans
Chesapeake Bay
-76.4395
-76.0922
35.0061
39.1405
2021-08-04
2021-08-10
Eastern Tropical South Pacific and Chesapeake Bay
0
BCO-DMO catalogue of parameters from CTD data from R/V Hugh R. Sharp cruise HRS2110 in the Chesapeake Bay during August 2021
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/869021.rdf
Name: Cast
Units: unitless
Description: <p>cast number</p>
http://lod.bco-dmo.org/id/dataset-parameter/869022.rdf
Name: Station
Units: unitless
Description: <p>station name</p>
http://lod.bco-dmo.org/id/dataset-parameter/869023.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>Datetime of cast; YYYYY-MM-DDTHH:MM:SSZ</p>
http://lod.bco-dmo.org/id/dataset-parameter/869024.rdf
Name: Date
Units: unitless
Description: <p>Date of cast; YYYY-MM-DD</p>
http://lod.bco-dmo.org/id/dataset-parameter/869025.rdf
Name: time_UTC
Units: unitless
Description: <p>Time of deployent, UTC; HH:MM:SS</p>
http://lod.bco-dmo.org/id/dataset-parameter/869026.rdf
Name: timeS
Units: seconds
Description: <p>Time, Elapsed</p>
http://lod.bco-dmo.org/id/dataset-parameter/869027.rdf
Name: depSM
Units: m
Description: <p>Depth [salt water]</p>
http://lod.bco-dmo.org/id/dataset-parameter/869028.rdf
Name: prDM
Units: decibars
Description: <p>Pressure, Digiquartz</p>
http://lod.bco-dmo.org/id/dataset-parameter/869029.rdf
Name: t090C
Units: oC
Description: <p>Temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/869030.rdf
Name: t190C
Units: oC
Description: <p>Temperature, 2</p>
http://lod.bco-dmo.org/id/dataset-parameter/869031.rdf
Name: c0S_per_m
Units: S/m
Description: <p>Conductivity</p>
http://lod.bco-dmo.org/id/dataset-parameter/869032.rdf
Name: c1S_per_m
Units: S/m
Description: <p>Conductivity, 2</p>
http://lod.bco-dmo.org/id/dataset-parameter/869033.rdf
Name: sal00_1
Units: PSU
Description: <p>Salinity, Practical</p>
http://lod.bco-dmo.org/id/dataset-parameter/869034.rdf
Name: sal11_1
Units: PSU
Description: <p>Salinity, Practical, 2</p>
http://lod.bco-dmo.org/id/dataset-parameter/869035.rdf
Name: timeQ
Units: seconds
Description: <p>Time, NMEA</p>
http://lod.bco-dmo.org/id/dataset-parameter/869036.rdf
Name: timeY
Units: seconds
Description: <p>Time, System</p>
http://lod.bco-dmo.org/id/dataset-parameter/869037.rdf
Name: latitude
Units: decimal degrees
Description: <p>Latitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/869038.rdf
Name: longitude
Units: decimal degrees
Description: <p>Longitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/869039.rdf
Name: svCM_1
Units: m/s
Description: <p>Sound Velocity</p>
http://lod.bco-dmo.org/id/dataset-parameter/869040.rdf
Name: flECO_AFL
Units: mg/m3
Description: <p>Fluorescence, WET Labs ECO-AFL/FL</p>
http://lod.bco-dmo.org/id/dataset-parameter/869041.rdf
Name: sbeox0ML_per_L
Units: ml/l
Description: <p>Oxygen, SBE 43</p>
http://lod.bco-dmo.org/id/dataset-parameter/869042.rdf
Name: sbox0Mm_per_Kg
Units: µmol/kg
Description: <p>Oxygen, SBE 43</p>
http://lod.bco-dmo.org/id/dataset-parameter/869043.rdf
Name: sbeox0Mg_per_L
Units: mg/l
Description: <p>Oxygen, SBE 43</p>
http://lod.bco-dmo.org/id/dataset-parameter/869044.rdf
Name: sigma_E00
Units: kg/m3
Description: <p>Density [sigma-theta]</p>
http://lod.bco-dmo.org/id/dataset-parameter/869045.rdf
Name: sigma_E11
Units: kgk/m3
Description: <p>Density, 2 [sigma-theta]</p>
http://lod.bco-dmo.org/id/dataset-parameter/869046.rdf
Name: potemp090C
Units: oC
Description: <p>Potential Temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/869047.rdf
Name: potemp190C
Units: oC
Description: <p>Potential Temperature, 2</p>
http://lod.bco-dmo.org/id/dataset-parameter/869048.rdf
Name: svCM_2
Units: m/s
Description: <p>Sound Velocity [Chen-Millero]</p>
http://lod.bco-dmo.org/id/dataset-parameter/869049.rdf
Name: sal00_2
Units: PSU
Description: <p>Salinity, Practical</p>
http://lod.bco-dmo.org/id/dataset-parameter/869050.rdf
Name: sal11_2
Units: PSU
Description: <p>Salinity, Practical, 2</p>
http://lod.bco-dmo.org/id/dataset-parameter/869051.rdf
Name: flag
Units: unitless
Description: <p>flag</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
92280
https://darchive.mblwhoilibrary.org/bitstream/1912/27994/1/dataset-868879_no2ox-omzs-ctd-data__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.868879.1
download
onLine
dataset
<p>Water samples were collected using a 12 x 10-liter&nbsp;Niskin bottle rosette sampler equipped with a conductivity, temperature, and pressure instrument package (SBE9, Sea-Bird Electronics, Bellevue, Washington, U.S.A.), a sensor for dissolved oxygen (SBE43, Sea-Bird), and a sensor for chlorophyll fluorescence (FluoroWetlabECO, AFL FL Sensor).</p>
Specified by the Principal Investigator(s)
<p>CTD data were processed with Seasave V 7.26.0.7.</p>
<p><strong>BCO-DMO Processing Notes:</strong><br />
- Standardized UTC time formatting;<br />
- Added a UTC datetime field in ISO 8601 format;<br />
- Renamed fields to remove all special characters except underscores.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
CTD Sea-Bird 9
CTD Sea-Bird 9
PI Supplied Instrument Name: CTD Sea-Bird 9 PI Supplied Instrument Description:A conductivity, temperature, and pressure instrument package (SBE9, Sea-Bird Electronics, Bellevue, Washington, U.S.A) attached to the Niskin bottle rosette used for sample collection. Instrument Name: CTD Sea-Bird 9 Instrument Short Name:CTD SBE 9 Instrument Description: The Sea-Bird SBE 9 is a type of CTD instrument package. The SBE 9 is the Underwater Unit and is most often combined with the SBE 11 Deck Unit (for real-time readout using conductive wire) when deployed from a research vessel. The combination of the SBE 9 and SBE 11 is called a SBE 911. The SBE 9 uses Sea-Bird's standard modular temperature and conductivity sensors (SBE 3 and SBE 4). The SBE 9 CTD can be configured with auxiliary sensors to measure other parameters including dissolved oxygen, pH, turbidity, fluorometer, altimeter, etc.). Note that in most cases, it is more accurate to specify SBE 911 than SBE 9 since it is likely a SBE 11 deck unit was used. more information from Sea-Bird Electronics Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/130/
Niskin bottle rosette sampler
Niskin bottle rosette sampler
PI Supplied Instrument Name: Niskin bottle rosette sampler PI Supplied Instrument Description:Water samples were collected using a 12 x 10 L Niskin bottle rosette sampler, which was equipped with CTD Sea-Bird 9, Sea-Bird SBE 43 Dissolved Oxygen, and Wet Labs ECO-AFL/FL Fluorometer sensors. Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
SBE43, Sea-Bird
SBE43, Sea-Bird
PI Supplied Instrument Name: SBE43, Sea-Bird PI Supplied Instrument Description:A dissolved oxygen sensor attached to the Niskin bottle rosette used for sample collection. Instrument Name: Sea-Bird SBE 43 Dissolved Oxygen Sensor Instrument Short Name:SBE-43 DO Instrument Description: The Sea-Bird SBE 43 dissolved oxygen sensor is a redesign of the Clark polarographic membrane type of dissolved oxygen sensors. more information from Sea-Bird Electronics Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0036/
FluoroWetlabECO, AFL FL Sensor
FluoroWetlabECO, AFL FL Sensor
PI Supplied Instrument Name: FluoroWetlabECO, AFL FL Sensor PI Supplied Instrument Description:A sensor for chlorophyll fluorescence attached to the Niskin bottle rosette used for sample collection. Instrument Name: Wet Labs ECO-AFL/FL Fluorometer Instrument Short Name:ECO AFL/FL Instrument Description: The Environmental Characterization Optics (ECO) series of single channel fluorometers delivers both high resolution and wide ranges across the entire line of parameters using 14 bit digital processing. The ECO series excels in biological monitoring and dye trace studies. The potted optics block results in long term stability of the instrument and the optional anti-biofouling technology delivers truly long term field measurements.
more information from Wet Labs Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0172/
Cruise: HRS2110
HRS2110
R/V Hugh R. Sharp
Community Standard Description
International Council for the Exploration of the Sea
R/V Hugh R. Sharp
vessel
HRS2110
Bess B. Ward
Princeton University
R/V Hugh R. Sharp
Community Standard Description
International Council for the Exploration of the Sea
R/V Hugh R. Sharp
vessel