http://lod.bco-dmo.org/id/dataset/868908
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2022-01-31
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
POM concentrations for carbon, nitrogen, and phosphorus from GO-SHIP Line C13.5/A13.5 in 2020
2022-01-31
publication
2022-01-31
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2022-02-01
publication
https://doi.org/10.26008/1912/bco-dmo.868908.1
Adam Martiny
University of California-Irvine
principalInvestigator
Nathan S. Garcia
University of California-Irvine
principalInvestigator
Tatsuro Tanioka
University of California-Irvine
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Martiny, A., Garcia, N. S., Tanioka, T., Fagan, A. J. (2022) POM concentrations for carbon, nitrogen, and phosphorus from GO-SHIP Line C13.5/A13.5 in 2020. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-01-31 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.868908.1 [access date]
Line C13.5/A13.5 POM concentrations Dataset Description: Methods and Sampling: <p><strong>POM sample collection:</strong><br />
General equipment preparation in the lab before shipment included an HCl bath (1.0 M HCl overnight) and milli-Q rinse for the 8 L carboys, tubing, and filter holders. Additionally, the carboys were autoclaved. The 25 mm, 0.7 μm GF/F filters and aluminum foil used to wrap the filters were combusted (500 °C for 5 hours) in aluminum foil packets to remove any traces of carbon present. The forceps that came in contact with the samples were wiped with 70% ethanol before and between uses. Seawater for the POM samples was collected from the onboard flow through underway system. The underway intake was located off the bow of the ship at a depth of approximately 5.3 m from the sea surface and the length of the pipe from the intake location to the output lab was 11.8 m. A 30 μm nylon mesh pre-filter was attached to the underway outlet for all standard samples to remove large plankton and particulates. Before sampling, the carboys used were rinsed twice with the pre-filtered underway seawater. Triplicate sampling occurred roughly at four-hour intervals with a shift forward of one hour each day. For example, if samples were taken at 01:00, 05:00, and 09:00 on one day, samples would be taken at 02:00, 06:00, and 10:00 the following day to avoid any diurnal bias. The water was vacuum filtered through combusted GF/F filters and the volume of seawater filtered was determined on a per station basis, ranging from 3-8 L. The volume was raised if the previous stations’ filtration times exhibited a decreasing trend and lowered if filtration time exhibited an increasing trend in an attempt to normalize the amount of collected material. The large sampling volume and initial rinsing steps limit the effect of a time delay from underway inlet to sampling station. After filtration, all POP triplicates were rinsed with approximately 2-5 mL of a 0.17 M Na2SO4 solution to remove dissolved phosphorus. All POM samples were folded in half with the top sides toward each other, sealed inside pieces of combusted aluminum foil, and stored in a -20 C freezer until analysis.</p>
<p><strong>POP assay:</strong><br />
The POP data were obtained using an ash/hydrolysis method and comparing the samples to a set of standard phosphorus concentrations (Lomas et al., 2010, <em>Biogeosciences</em>, <strong>7</strong>(2), 695-710, doi:10.5194/bg-7-695-2010). The sample filters were unfolded and placed face up into acid-bathed and combusted scintillation vials. Along with each set of samples, 10 different volumes (ranging 0 - 0.5 mL) of 0.1 M KH2PO4 solution were added to scintillation vials. 2 mL of a 0.017 M MgSO4 drying solution was added to each scintillation vial and then all vials were placed into an 80-90 °C oven overnight to dry. After drying, the vials were heated at 500 °C for 2 hours, then left to cool before adding 5 mL of 0.2 M HCl to each vial and being returned to the 80-90 C oven for 30 minutes after being brought up to temperature. The solutions were then transferred to 15 mL glass centrifuge tubes (prepared in the same way as the scintillation vials). The sample vials were rinsed with 5 mL of milli-Q each, which was also added to the centrifuge tubes. 1 mL of a mixed reagent containing 2:5:1:2 parts Ammonium Molybdate ((NH4)6Mo7O24), 5.0 N H2SO4, Potassium Antimonyl Tartrate (C8H4K2O12Sb2), and Ascorbic Acid (C6H8O6) respectively was added to each centrifuge tube in 30-second intervals. Each of the sample tubes was centrifuged to isolate any glass fibers that could interfere with the absorbance reading. Lastly, after allowing the mixed reagent to react for exactly 30 min, the standards and samples were analyzed in 30-second intervals in a spectrophotometer at an 885 nm wavelength using a blank of ~0.1 M HCl solution and rinsing the cuvette with the blank solution between measurements.</p>
<p><strong>POC/PON assay:</strong><br />
POC and PON measurements were analyzed both in-lab at UCI. The preparation for all samples was the same; the samples were each removed from their foil packets and placed into acid-bathed and combusted scintillation vials and dried in a 55 °C oven overnight. The scintillation vials were then placed in a desiccator containing a beaker of 12 M HCl overnight before being dried at 55 °C at least overnight once more. Samples sent to UCSB were then capped and shipped to the lab. Samples analyzed at UCI were packed into tin packets alongside Atropine (C17H23NO3) standards (ranging 0.2-1.5 mg) and measured using a Flash EA elemental analyzer.</p>
<p>Associated GO-SHIP A13.5 underway and bottle datasets can be found on the CLIVAR and Carbon Hydrographic Data Office Section A13.5 homepage: <a href="https://cchdo.ucsd.edu/cruise/33RO20200321" target="_blank">https://cchdo.ucsd.edu/cruise/33RO20200321</a></p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1848576 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1848576
completed
Adam Martiny
University of California-Irvine
949-824-9713
Earth System Science & Ecology and Evolutionary Biology 3208 Croul Hall
Irvine
CA
92697
USA
amartiny@uci.edu
pointOfContact
Nathan S. Garcia
University of California-Irvine
Irvine
CA
USA
n8garcia@gmail.com
pointOfContact
Tatsuro Tanioka
University of California-Irvine
Irvine
CA
USA
tatsurt@uci.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Sample
Station
Latitude
Longitude
ISO_DateTime_UTC
Vol_CN1
Vol_CN2
Vol_CN3
Vol_POP1
Vol_POP2
Vol_POP3
POC_Rep1
POC_Rep2
POC_Rep3
PON_Rep1
PON_Rep2
PON_Rep3
POP_Rep1
POP_Rep2
POP_Rep3
CN FlashEA 1112 Elemental Analyzer (Thermo Scientific, Waltham, Massachusetts)
Genesys 10vis spectrophotometer (#840-208100, Thermo Scientific, Waltham, Massachusetts)
theme
None, User defined
sample identification
station
latitude
longitude
ISO_DateTime_UTC
volume
particulate organic Carbon (POC)
particulate organic nitrogen
particulate organic phosphorus
featureType
BCO-DMO Standard Parameters
Elemental Analyzer
Spectrophotometer
instrument
BCO-DMO Standard Instruments
RB2002
service
Deployment Activity
Meridional section through the Eastern part of the South Atlantic
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Convergence: RAISE: Linking the adaptive dynamics of plankton with emergent global ocean biogeochemistry
https://osprey.bco-dmo.org/project/764270
Convergence: RAISE: Linking the adaptive dynamics of plankton with emergent global ocean biogeochemistry
<p><em>NSF Award Abstract:</em><br />
Due to their sheer abundance and high activity, microorganisms have the potential to greatly influence how ecosystems are affected by changes in their environment. However, descriptions of microbial physiology and diversity are local and highly complex and thus rarely considered in Earth System Models. Thus, the researchers focus on a convergence research framework that can qualitatively and quantitatively integrate eco-evolutionary changes in microorganisms with global biogeochemistry. Here, the investigators will develop an approach that integrates the knowledge and tools of biologists, mathematicians, engineers, and geoscientists to understand the link between the ocean nutrient and carbon cycles. The integration of data and knowledge from diverse fields will provide a robust, biologically rich, and computationally efficient prediction for the variation in plankton resource requirements and the biogeochemical implications, addressing a fundamental challenge in ocean science. In addition, the project can serve as a road map for many other research groups facing a similar lack of convergence between biology and geoscience.</p>
<p>Traditionally, the cellular elemental ratios of Carbon, Nitrogen, and Phosphorus (C:N:P) of marine communities have been considered static at Redfield proportions but recent studies have demonstrated strong latitudinal variation. Such regional variation may have large - but poorly constrained - implications for marine biodiversity, biogeochemical functioning, and atmospheric carbon dioxide levels. As such, variations in ocean community C:N:P may represent an important biological feedback. Here, the investigators propose a convergence research framework integrating cellular and ecological processes controlling microbial resource allocations with an Earth System model. The approach combines culture experiments and omics measurements to provide a molecular understanding of cellular resource allocations. Using a mathematical framework of increasing complexity describing communicating, moving demes, the team will quantify the extent to which local mixing, environmental heterogeneity and evolution lead to systematic deviations in plankton resource allocations and C:N:P. Optimization tools from engineering science will be used to facilitate the quantitative integration of models and observations across a range of scales and complexity levels. Finally, global ocean modeling will enable understanding of how plankton resource use impacts Earth System processes. By integrating data and knowledge across fields, scales and complexity, the investigators will develop a robust link between variation in plankton C:N:P and global biogeochemical cycles.</p>
Ocean Stoichiometry
largerWorkCitation
project
eng; USA
oceans
Meridional section through the Eastern part of the South Atlantic
-73.507
17.3055
-41.4917
34.5045
2020-03-21
2020-04-16
0
BCO-DMO catalogue of parameters from POM concentrations for carbon, nitrogen, and phosphorus from GO-SHIP Line C13.5/A13.5 in 2020
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/868941.rdf
Name: Sample
Units: unitless
Description: <p>Sample identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/868942.rdf
Name: Station
Units: unitless
Description: <p>Station number</p>
http://lod.bco-dmo.org/id/dataset-parameter/868943.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitude; positive values = North</p>
http://lod.bco-dmo.org/id/dataset-parameter/868944.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitude; positive values = East</p>
http://lod.bco-dmo.org/id/dataset-parameter/868945.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>Date and time of collection (UTC) in ISO8601 format yyyy-mm-ddTHH:MMZ</p>
http://lod.bco-dmo.org/id/dataset-parameter/868946.rdf
Name: Vol_CN1
Units: liters (L)
Description: <p>Filtration volume for POC and PON samples for replicate 1</p>
http://lod.bco-dmo.org/id/dataset-parameter/868947.rdf
Name: Vol_CN2
Units: liters (L)
Description: <p>Filtration volume for POC and PON samples for replicate 2</p>
http://lod.bco-dmo.org/id/dataset-parameter/868948.rdf
Name: Vol_CN3
Units: liters (L)
Description: <p>Filtration volume for POC and PON samples for replicate 3</p>
http://lod.bco-dmo.org/id/dataset-parameter/868949.rdf
Name: Vol_POP1
Units: liters (L)
Description: <p>Filtration volume for POP sample for replicate 1</p>
http://lod.bco-dmo.org/id/dataset-parameter/868950.rdf
Name: Vol_POP2
Units: liters (L)
Description: <p>Filtration volume for POP sample for replicate 2</p>
http://lod.bco-dmo.org/id/dataset-parameter/868951.rdf
Name: Vol_POP3
Units: liters (L)
Description: <p>Filtration volume for POP sample for replicate 3</p>
http://lod.bco-dmo.org/id/dataset-parameter/868952.rdf
Name: POC_Rep1
Units: micromoles per liter (uM)
Description: <p>Particulate organic carbon < 30 um for replicate 1</p>
http://lod.bco-dmo.org/id/dataset-parameter/868953.rdf
Name: POC_Rep2
Units: micromoles per liter (uM)
Description: <p>Particulate organic carbon < 30 um for replicate 2</p>
http://lod.bco-dmo.org/id/dataset-parameter/868954.rdf
Name: POC_Rep3
Units: micromoles per liter (uM)
Description: <p>Particulate organic carbon < 30 um for replicate 3</p>
http://lod.bco-dmo.org/id/dataset-parameter/868955.rdf
Name: PON_Rep1
Units: micromoles per liter (uM)
Description: <p>Particulate organic nitrogen < 30 um for replicate 1</p>
http://lod.bco-dmo.org/id/dataset-parameter/868956.rdf
Name: PON_Rep2
Units: micromoles per liter (uM)
Description: <p>Particulate organic nitrogen < 30 um for replicate 2</p>
http://lod.bco-dmo.org/id/dataset-parameter/868957.rdf
Name: PON_Rep3
Units: micromoles per liter (uM)
Description: <p>Particulate organic nitrogen < 30 um for replicate 3</p>
http://lod.bco-dmo.org/id/dataset-parameter/868958.rdf
Name: POP_Rep1
Units: nanomoles per liter (nM)
Description: <p>Particulate organic phosphorus < 30 um for replicate 1</p>
http://lod.bco-dmo.org/id/dataset-parameter/868959.rdf
Name: POP_Rep2
Units: nanomoles per liter (nM)
Description: <p>Particulate organic phosphorus < 30 um for replicate 2</p>
http://lod.bco-dmo.org/id/dataset-parameter/868960.rdf
Name: POP_Rep3
Units: nanomoles per liter (nM)
Description: <p>Particulate organic phosphorus < 30 um for replicate 3</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
13795
https://darchive.mblwhoilibrary.org/bitstream/1912/27969/1/dataset-868908_line-c135a135-pom-concentrations__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.868908.1
download
onLine
dataset
<p><strong>POM sample collection:</strong><br />
General equipment preparation in the lab before shipment included an HCl bath (1.0 M HCl overnight) and milli-Q rinse for the 8 L carboys, tubing, and filter holders. Additionally, the carboys were autoclaved. The 25 mm, 0.7 μm GF/F filters and aluminum foil used to wrap the filters were combusted (500 °C for 5 hours) in aluminum foil packets to remove any traces of carbon present. The forceps that came in contact with the samples were wiped with 70% ethanol before and between uses. Seawater for the POM samples was collected from the onboard flow through underway system. The underway intake was located off the bow of the ship at a depth of approximately 5.3 m from the sea surface and the length of the pipe from the intake location to the output lab was 11.8 m. A 30 μm nylon mesh pre-filter was attached to the underway outlet for all standard samples to remove large plankton and particulates. Before sampling, the carboys used were rinsed twice with the pre-filtered underway seawater. Triplicate sampling occurred roughly at four-hour intervals with a shift forward of one hour each day. For example, if samples were taken at 01:00, 05:00, and 09:00 on one day, samples would be taken at 02:00, 06:00, and 10:00 the following day to avoid any diurnal bias. The water was vacuum filtered through combusted GF/F filters and the volume of seawater filtered was determined on a per station basis, ranging from 3-8 L. The volume was raised if the previous stations’ filtration times exhibited a decreasing trend and lowered if filtration time exhibited an increasing trend in an attempt to normalize the amount of collected material. The large sampling volume and initial rinsing steps limit the effect of a time delay from underway inlet to sampling station. After filtration, all POP triplicates were rinsed with approximately 2-5 mL of a 0.17 M Na2SO4 solution to remove dissolved phosphorus. All POM samples were folded in half with the top sides toward each other, sealed inside pieces of combusted aluminum foil, and stored in a -20 C freezer until analysis.</p>
<p><strong>POP assay:</strong><br />
The POP data were obtained using an ash/hydrolysis method and comparing the samples to a set of standard phosphorus concentrations (Lomas et al., 2010, <em>Biogeosciences</em>, <strong>7</strong>(2), 695-710, doi:10.5194/bg-7-695-2010). The sample filters were unfolded and placed face up into acid-bathed and combusted scintillation vials. Along with each set of samples, 10 different volumes (ranging 0 - 0.5 mL) of 0.1 M KH2PO4 solution were added to scintillation vials. 2 mL of a 0.017 M MgSO4 drying solution was added to each scintillation vial and then all vials were placed into an 80-90 °C oven overnight to dry. After drying, the vials were heated at 500 °C for 2 hours, then left to cool before adding 5 mL of 0.2 M HCl to each vial and being returned to the 80-90 C oven for 30 minutes after being brought up to temperature. The solutions were then transferred to 15 mL glass centrifuge tubes (prepared in the same way as the scintillation vials). The sample vials were rinsed with 5 mL of milli-Q each, which was also added to the centrifuge tubes. 1 mL of a mixed reagent containing 2:5:1:2 parts Ammonium Molybdate ((NH4)6Mo7O24), 5.0 N H2SO4, Potassium Antimonyl Tartrate (C8H4K2O12Sb2), and Ascorbic Acid (C6H8O6) respectively was added to each centrifuge tube in 30-second intervals. Each of the sample tubes was centrifuged to isolate any glass fibers that could interfere with the absorbance reading. Lastly, after allowing the mixed reagent to react for exactly 30 min, the standards and samples were analyzed in 30-second intervals in a spectrophotometer at an 885 nm wavelength using a blank of ~0.1 M HCl solution and rinsing the cuvette with the blank solution between measurements.</p>
<p><strong>POC/PON assay:</strong><br />
POC and PON measurements were analyzed both in-lab at UCI. The preparation for all samples was the same; the samples were each removed from their foil packets and placed into acid-bathed and combusted scintillation vials and dried in a 55 °C oven overnight. The scintillation vials were then placed in a desiccator containing a beaker of 12 M HCl overnight before being dried at 55 °C at least overnight once more. Samples sent to UCSB were then capped and shipped to the lab. Samples analyzed at UCI were packed into tin packets alongside Atropine (C17H23NO3) standards (ranging 0.2-1.5 mg) and measured using a Flash EA elemental analyzer.</p>
<p>Associated GO-SHIP A13.5 underway and bottle datasets can be found on the CLIVAR and Carbon Hydrographic Data Office Section A13.5 homepage: <a href="https://cchdo.ucsd.edu/cruise/33RO20200321" target="_blank">https://cchdo.ucsd.edu/cruise/33RO20200321</a></p>
Specified by the Principal Investigator(s)
<p><strong>Data Processing:</strong><br />
Matlab v2021a and R4.1.0 were used for data processing and wrangling.<br />
Blank values in this dataset are displayed as "nd" for "no data."</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
CN FlashEA 1112 Elemental Analyzer (Thermo Scientific, Waltham, Massachusetts)
CN FlashEA 1112 Elemental Analyzer (Thermo Scientific, Waltham, Massachusetts)
PI Supplied Instrument Name: CN FlashEA 1112 Elemental Analyzer (Thermo Scientific, Waltham, Massachusetts) Instrument Name: Elemental Analyzer Instrument Short Name: Instrument Description: Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/
Genesys 10vis spectrophotometer (#840-208100, Thermo Scientific, Waltham, Massachusetts)
Genesys 10vis spectrophotometer (#840-208100, Thermo Scientific, Waltham, Massachusetts)
PI Supplied Instrument Name: Genesys 10vis spectrophotometer (#840-208100, Thermo Scientific, Waltham, Massachusetts) Instrument Name: Spectrophotometer Instrument Short Name:Spectrophotometer Instrument Description: An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/
Cruise: RB2002
RB2002
Community Standard Description
International Council for the Exploration of the Sea
NOAA Ship Ronald H. Brown
vessel
RB2002
Leticia Barbero
National Oceanic and Atmospheric Administration
Community Standard Description
International Council for the Exploration of the Sea
NOAA Ship Ronald H. Brown
vessel