http://lod.bco-dmo.org/id/dataset/869414
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2022-02-08
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Nutrients measured in porewater from sediments collected at Guaymas Basin from R/V JOIDES Resolution IODP-385 cruise in the Guaymas Basin from September to June, 2019.
2022-08-12
publication
2022-08-12
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2022-08-12
publication
https://doi.org/10.26008/1912/bco-dmo.869414.1
Virginia P. Edgcomb
Woods Hole Oceanographic Institution
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Edgcomb, V. P., Teske, A. (2022) Nutrients measured in porewater from sediments collected at Guaymas Basin from R/V JOIDES Resolution IODP-385 cruise in the Guaymas Basin from September to June, 2019. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-08-12 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.869414.1 [access date]
Sediment porewater nutrients Dataset Description: Methods and Sampling: <p>Sediments samples dedicated to nutrient analyses recovered from 8 drilling sites from up to 427 meter below seafloor. Two ~40ml samples of sediment were placed in 50 ml Falcon tubes and were centrifuged at 3000 rpm for 15 minutes to separate porewater from the sediment. The porewater was collected into vials for dissolved inorganic carbon (DIC), dissolved organic carbon (DOC), NOx, and total nitrogen (TN). Samples were stored at 4oC.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2048489 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2048489
completed
Virginia P. Edgcomb
Woods Hole Oceanographic Institution
508-289-3734
Department of Geology and Geophysics 220 McLean Lab, MS #8
Woods Hole
MA
02543
USA
vedgcomb@whoi.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Sample_ID
Depth
Latitude
Longitude
ISO_DateTime_UTC
Temperature
Dissolved_Organic_Carbon
Dissolved_Inorganic_Carbon
Total_Nitrogen
NO2_NO_as_N_um
NO2_NO_as_N
Notes
Costech 1040 CHNOS Elemental Combustion system
Shimadzu TOC/TN analyzer
theme
None, User defined
sample identification
depth
latitude
longitude
ISO_DateTime_UTC
water temperature at measurement depth
dissolved organic Carbon
dissolved inorganic Carbon
Nitrogen
nitrate plus nitrite
comments
featureType
BCO-DMO Standard Parameters
Nutrient Autoanalyzer
Total Nitrogen Analyzer
instrument
BCO-DMO Standard Instruments
IODP-385
service
Deployment Activity
Guaymas Basin 27 N 111 W
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: IODP-enabled Insights into Fungi and Their Metabolic Interactions with Other Microorganisms in Deep Subsurface Hydrothermal Sediments
https://www2.whoi.edu/site/edgcomblab/research/deep-marine-subsurface-eukaryotes/
Collaborative Research: IODP-enabled Insights into Fungi and Their Metabolic Interactions with Other Microorganisms in Deep Subsurface Hydrothermal Sediments
<p><strong>NSF Award Abstract</strong></p>
<p>The marine subsurface is one of the least explored habitats on Earth. International Ocean Discovery Program (IODP) Expedition 385 drilled into the seafloor in Guaymas Basin, Mexico, and was the first to drill directly into subsurface sediments and sediment-hosted basalt sill intrusions of an active hydrothermal basin. This expedition provides a direct microbiological window into a deep hydrothermal biosphere across an active plate spreading center where complex hydrocarbons are generated by heating of buried organic matter under high temperature and pressure. Mounting evidence suggests that Fungi constitute an active and ecologically important fraction of the subsurface biosphere community. This is especially true in organic-rich continental margin sediments that are ideal for colonization by aerobic and anaerobic Fungi, where fungal activities may contribute significantly to nutrient cycling. Major knowledge gaps in our knowledge of subsurface microbiota preclude our ability to estimate their full impact: how active Fungi are distributed along temperature and depth gradients, the range of substrates utilized by active cells, and how Fungi may cooperate with bacteria in degradation of complex organic matter, including hydrocarbons. Fungi are known to participate in degradation of refractory organics and cycling of metals and to produce novel metabolites with interesting properties. This project informs us on origins of different lineages of microbial life on Earth, the extent of marine subsurface carbon cycling, limits of life, how life adapts to environmental change, and the potential for Fungi to accelerate the biodegradation of complex hydrocarbons. Given the extent of the potential subsurface biosphere, Fungi likely play an important role in global nutrient cycling. The culture collection of fungal isolates created by this project will be available for exploration of their ecology and novel properties by other interested researchers, and may also yield insights into basal fungal lineages. These biogeochemical and potential evolutionary outcomes are of great interest to other research disciplines, educators, and students alike. The project’s K-16 education program capitalizes on programs aimed at increasing involvement of under-represented undergraduate populations in research. High school students, undergraduates, a graduate student, and postdoc are involved in the research. An art-in-science project with a local high school is being displayed at the community library along with education materials on marine Fungi and their ecological roles.</p>
<p>This project examines how abundance, diversity and distribution of Fungi and co-inhabiting bacteria and archaea changes in subsurface sediment samples exhibiting a wide range of in situ temperatures and pressure, what the active fraction of cells is along these gradients, and whether/how Fungi impact carbon cycling in this biosphere by interacting metabolically with bacteria to break down hydrocarbon substrates. The project is assessing the activities of in situ microorganisms in this active hydrothermal subsurface biosphere using a cutting-edge combination of molecular approaches and culture-based studies of enrichments and microbial isolates applied to an extensive collection of samples from 8 sites in Guaymas Basin varying in temperature profile, presence of old, buried magmatic sills, and geochemical conditions. The investigators are examining 1) marker genes and metagenomes of sorted active cells using new bioorthogonal non-canonical amino acid tagging (BONCAT) approaches, 2) the distribution of bacterial, archaeal, and fungal cells and their marker genes along depth and geochemical gradients using microscopy, ‘meta-omics’ and lipid biomarker analyses, 3) substrate usage by fungal isolates, 4) metabolite pools, nutrients, and hydrocarbons with depth, and 5) fungal metabolism of complex organics (and syntrophies between Fungi and Bacteria) using time-course stable isotope probing of RNA from culture-based studies coupled with analyses of expressed genes and pools of metabolites.</p>
<p><strong>Broader Impacts </strong></p>
<p>The proposed project can transform our understanding of microbial life in the sedimented marine subsurface biosphere because active mycobiota would have implications for deep carbon budgets. Our culture collection is estimated to generate hundreds of new strains of fungi that will be available for exploration of their ecology and novel properties by interested researchers, and may also yield insights into basal fungal lineages. These biogeochemical and potential evolutionary outcomes are of great interest to other research disciplines, educators, and students alike. Our proposed K-16 education program capitalizes on programs aimed at increasing involvement of under-represented undergraduate populations in research. High school students, undergraduates (4 per year), a graduate student, and postdoc will be involved. The PI and an art teacher at a local high school will teach an art-in-science unit. The product (a large quilt of art inspired by fungal cultures) will be displayed at the community library along with education materials on marine fungi and their ecological roles. The project involves two international collaborators, and will partially support 4 principal investigators at three institutions, including two early career researchers, Roland Hatzenpichler and Paraskevi Mara.</p>
<p><strong>Additional Shipboard Data from the Expedition</strong></p>
<p>The IODP page for Guaymas Expedition 385 contains multiple site chapters, one for each drilling site (U1545-U1552); each site chapter contains sections about petrology, sedimentology, porewater chemistry, etc.; data tables are embedded into the site chapters. The citation and access DOI for this resource is as follows: </p>
<p>Teske, A., Lizarralde, D., & Höfig, T. W. (2021). Guaymas Basin Tectonics and Biosphere. Proceedings of the International Ocean Discovery Program. <a href="https://doi.org/10.14379/iodp.proc.385.2021" rel="noopener noreferrer" target="_blank">https://doi.org/10.14379/iodp.proc.385.2021</a></p>
IODP insights Fungi
largerWorkCitation
project
eng; USA
oceans
Guaymas Basin 27 N 111 W
-111.889
-111.22
27.2064
27.6374
2019-10-04
2019-11-10
Guaymas Basin, Gulf of California, Mexico
0
BCO-DMO catalogue of parameters from Nutrients measured in porewater from sediments collected at Guaymas Basin from R/V JOIDES Resolution IODP-385 cruise in the Guaymas Basin from September to June, 2019.
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/870191.rdf
Name: Sample_ID
Units: unitless
Description: <p>Sample ID</p>
http://lod.bco-dmo.org/id/dataset-parameter/870192.rdf
Name: Depth
Units: meters (m)
Description: <p>Sample depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/870193.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitude of sampling location, south is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/870194.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitude of sampling location, west is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/870195.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>Date and time of sampling in ISO format (UTC timezone)</p>
http://lod.bco-dmo.org/id/dataset-parameter/870196.rdf
Name: Temperature
Units: degrees Celsius (°C)
Description: <p>Temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/870197.rdf
Name: Dissolved_Organic_Carbon
Units: mg/L
Description: <p>measurement of Dissolved Organic Carbon</p>
http://lod.bco-dmo.org/id/dataset-parameter/870198.rdf
Name: Dissolved_Inorganic_Carbon
Units: mg/L
Description: <p>measurement of Dissolved Inorganic Carbon</p>
http://lod.bco-dmo.org/id/dataset-parameter/870199.rdf
Name: Total_Nitrogen
Units: mg/L
Description: <p>measurement of Total Nitrogen</p>
http://lod.bco-dmo.org/id/dataset-parameter/870200.rdf
Name: NO2_NO_as_N_um
Units: uM
Description: <p>measurement of nitrate and nitrite a nitrogen</p>
http://lod.bco-dmo.org/id/dataset-parameter/870201.rdf
Name: NO2_NO_as_N
Units: mg/L -N
Description: <p>measurement of nitrate and nitrite a nitrogen</p>
http://lod.bco-dmo.org/id/dataset-parameter/870202.rdf
Name: Notes
Units: units
Description: <p>description</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
7681
https://darchive.mblwhoilibrary.org/bitstream/1912/29220/1/dataset-869414_porewater-nutrients__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.869414.1
download
onLine
dataset
<p>Sediments samples dedicated to nutrient analyses recovered from 8 drilling sites from up to 427 meter below seafloor. Two ~40ml samples of sediment were placed in 50 ml Falcon tubes and were centrifuged at 3000 rpm for 15 minutes to separate porewater from the sediment. The porewater was collected into vials for dissolved inorganic carbon (DIC), dissolved organic carbon (DOC), NOx, and total nitrogen (TN). Samples were stored at 4oC.</p>
Specified by the Principal Investigator(s)
<p>Colorimetric determinations of ammonium, nitrate, were performed using a OI Analytical Flow Solutions IV auto analyzer. Total nitrogen and carbon (weight %) are measured with a Costech 1040 CHNOS Elemental Combustion system. Filtered porewater samples were analyzed for dissolved organic carbon (DOC) and total dissolved nitrogen (TDN) using a Shimadzu TOC/TN analyzer. Dissolved organic nitrogen (DON) was calculated by subtracting TDN from the sum of the inorganic nitrogen species.&nbsp; &nbsp;</p>
<p>All nutrient analyses were performed at Wetland Biogeochemistry Analytical Services, LSU, LA.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Costech 1040 CHNOS Elemental Combustion system
Costech 1040 CHNOS Elemental Combustion system
PI Supplied Instrument Name: Costech 1040 CHNOS Elemental Combustion system Instrument Name: Nutrient Autoanalyzer Instrument Short Name:Nutrient Autoanalyzer Instrument Description: Nutrient Autoanalyzer is a generic term used when specific type, make and model were not specified. In general, a Nutrient Autoanalyzer is an automated flow-thru system for doing nutrient analysis (nitrate, ammonium, orthophosphate, and silicate) on seawater samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/
Shimadzu TOC/TN analyzer
Shimadzu TOC/TN analyzer
PI Supplied Instrument Name: Shimadzu TOC/TN analyzer Instrument Name: Total Nitrogen Analyzer Instrument Short Name:TN analyzer Instrument Description: A unit that accurately determines the nitrogen concentrations of organic compounds typically by detecting and measuring its combustion product (NO). See description document at: http://bcodata.whoi.edu/LaurentianGreatLakes_Chemistry/totalnit.pdf Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/
Cruise: IODP-385
IODP-385
R/V JOIDES Resolution
Community Standard Description
International Council for the Exploration of the Sea
R/V JOIDES Resolution
vessel
IODP-385
Andreas Teske
R/V JOIDES Resolution
Community Standard Description
International Council for the Exploration of the Sea
R/V JOIDES Resolution
vessel