19:I[42426,["/_next/static/immutable/chunks/1xctgzqre-wsy.js","/_next/static/immutable/chunks/06_-wz6pdtp2b.js","/_next/static/immutable/chunks/30e52g49hg40p.js","/_next/static/immutable/chunks/02yq6tul2z-oh.js","/_next/static/immutable/chunks/10bx_ec2vj7n_.js","/_next/static/immutable/chunks/33bfn03fo8xnt.js","/_next/static/immutable/chunks/0tfjf78scu61b.js","/_next/static/immutable/chunks/31xnvej4n1bi9.js"],"default"] 1a:T494,

In each trial, 25 oysters from each stock were placed in labelled petri dishes within 15 38-L tanks (100 oysters per tank) with aerated artificial seawater (using Instant Ocean Reef Crystals Reef Salt, Blacksburg, Virginia) at 25 °C and salinities of 2.0, 4.0, 20.0, 38.0 and 44.0; each salinity treatment was replicated three times (5 salinity treatments × 3 replicate groups = 15 tanks). The temperature of each tank was maintained using Hydor 100W submersible glass aquarium heaters. Oxygen was supplied to each aquarium via air pump/stone, and tanks were heated to approx. 25 oC. Temperature and salinity from each tank were recorded daily. Nutrient (NO2, NO2, NH3/NH4) concentrations in each tank were measured approximately weekly. Oysters were fed daily with Shellfish Diet 1800® at approximately 3mL/100 oysters unless the water was cloudy (i.e., food from the previous day hadn’t been consumed). Trial 2 is a repeat of the same experiment conducted in Nov/Dec 2018 using the same oyster stock. The only difference is that three aquaria of salinity 20 were added with no airstone to compare oyster mortality in low and high oxygen concentrations.

1b:Taa0,NSF Award Abstract: The project focuses on understanding how oysters from different regions in the Gulf of Mexico (GOM) will be affected by ongoing changes to salinity and temperature caused by a warming climate, altered rainfall patterns, and changes in land use in this region. The oyster fishery in the GOM provides more than half of the national total, and these oyster reefs provide water filtration, shoreline stability, and critical habitat for other species. By investigating how present-day oyster populations respond to changes in temperature and salinity, this research will provide valuable information to ecologists, conservation biologists, state managers, and small-scale farmers as they plan for the effects of future changes in the environment. By identifying populations of oysters that are the most resilient to environmental changes, this research will identify potential source populations that may be used in future restoration efforts aimed at declining populations. Additionally this project will integrate research and education through a semester-long immersive research experience. Three undergraduates, two graduate students, and a postdoctoral scholar will be mentored. Public outreach will be conducted through development of middle school lesson plans, public lectures, and dissemination of results to managers. This research will test whether eastern oysters (Crassotrea virginica) in the Gulf of Mexico are locally adapted to salinity, and whether combined changes in salinity and temperature will have synergistic effects on oyster physiology and ecology. Both temperature and salinity are expected to change rapidly in the Gulf of Mexico over the coming century, due to effects of climate change, altered rainfall patterns, and changes to coastal hydrology. Oysters are a critical habitat-forming species in this region, and while they inhabit a wide range of salinities, preliminary data indicate substantial differences in salinity tolerances among populations. This research will integrate genomic and physiological tools with controlled rearing experiments to (1) quantify genomic variation among populations of C. virginica, (2) test for local adaptation, and (3) measure physiological rates and gene expression as a function of temperature and salinity. The investigators will then use these data to build estuary-specific dynamic energy budget models, linking salinity and temperature to population growth. This project will provide critical support to ongoing restoration efforts, identifying adaptive variation among populations targeted for supportive breeding programs and informing management decisions in the face of ongoing ocean warming and changes to coastal hydrology.6:["$","$L19",null,{"dataset":{"_id":"870248","description":"

A detailed description of methods was written by Marshall et al. (2021). In summary, mortality of oyster spat spawned from four different broodstocks was measured when exposed to five different salinities (without acclimation) under controlled laboratory conditions. Oyster broodstocks were sourced from two populations in Louisiana (Calcasieu Lake; 29°50′58′′N, 93°17′1′′W, and Vermilion Bay; 29°34′47′′N, 92°2′4′′W) and two populations in Texas (Packery Channel; 27°37′38′′N, 97°13′59′′W, and Aransas Bay; 28°7′38′′N, 96°59′8′′W). This salinity tolerance experiment was conducted in November–December 2018 (Trial 1) and March–April 2019 (Trial 2) at Texas A&M University-Corpus Christi.

\n","problem":"","acquisition":"$1a","processing":"

Oyster spat heights were measured to the nearest millimeter during each experiment using calipers. Samples of 75 spat were measured in both Trial 1 and Trial 2.

BCO-DMO processing:
\n* Merged tables of trial 1 and trial 2
\n* Added column Trial 1 and Trial 2 to be able to differentiate between the experiments.
\n* Added ISO_DateTime_UTC column based on date and time (in CDT) columns.

\n","bcodmoProcessing":"","title":"Salinity tolerance of oysters without acclimation in lab conditions: shell heights","nickname":"Salinity tolerance without acclimation: Shell Heights","dataUrl":"","currentState":"Final no updates expected","currentStateId":"7","validated":true,"restricted":false,"version":"1","version_date":"2023-02-24T05:00:00.000Z","abstract":"The mortality of oyster spat spawned from four different wild broodstocks (first filial generation) was measured when exposed to five different salinities (without acclimation) under controlled laboratory conditions. Oyster broodstocks were sourced from two populations in Louisiana (Calcasieu Lake; 29°50′58′′N, 93°17′1′′W, and Vermilion Bay; 29°34′47′′N, 92°2′4′′W) and two populations in Texas (Packery Channel; 27°37′38′′N, 97°13′59′′W, and Aransas Bay; 28°7′38′′N, 96°59′8′′W). Mortality was recorded in oyster spat that were exposed to salinities of 2, 4, 20, 38 and 44 without acclimation under laboratory conditions. Changes in water quality and spat size were also recorded.","citation":"Pollack, J. B., Palmer, T., Breaux, N., Kelly, M., La Peyre, J. (2023) Salinity tolerance of oysters without acclimation in lab conditions: shell heights. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-02-24 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.870248.1 [access date]","citationParts":{"identifier":"doi:10.26008/1912/bco-dmo.870248.1","versionDate":"2023-02-24","versionId":"1","year":"2023","title":"Salinity tolerance of oysters without acclimation in lab conditions: shell heights","authors":"Pollack, J. B., Palmer, T., Breaux, N., Kelly, M., La Peyre, J.","publisher":"Biological and Chemical Oceanography Data Management Office (BCO-DMO)."},"doi":"10.26008/1912/bco-dmo.870248.1","release_date":null,"field_dataset_location":"","temporal_extent":{"start":"2018-12-14T05:00:00.000Z","end":"2019-03-26T04:00:00.000Z"},"temporal":{"resolution":"time:unitDay","type":"time:Interval","start":{"value":"1544763600","label":"2018-12-14"},"end":{"value":"1553572800","label":"2019-03-26"}},"spatial_extent":{"shape":"polygon","center":{"latitude":28.746665,"longitude":-94.63375},"wkt":"POLYGON ((-97.23306 27.62722, -92.03444 27.62722, -92.03444 29.86611, -97.23306 29.86611, -97.23306 27.62722))","north":29.86611,"east":-92.03444,"south":27.62722,"west":-97.23306},"dataset_type":["experimental"],"keywords":["oceans"],"data_files":[{"id":"903614","title":"shell_heights.csv","url":"https://datadocs.bco-dmo.org/dataset/870248/file/7DDMmypcQG531L/shell_heights.csv","showTableUrl":"https://www.bco-dmo.org/dataset/870248/data/view/903614","description":"Primary data file for dataset ID 870248","filename":"shell_heights.csv","bytesize":8594,"checksum":{"value":"4339046cf2e807df78e19475133b73fb","Algorithm":{"_id":"190","name":"MD5"}},"mimetype":"text/csv","fileType":{"_id":"192","name":"Comma Separated Values (.csv)"},"isErddap":"","date":"","isPublicDuringEmbargo":false,"primaryDataFile":true,"showTable":true,"restricted":false}],"supplemental_files":[],"roles":[{"role":{"_id":"138","name":"Principal Investigator"},"performedBy":{"_id":"764994","name":"Morgan Kelly"},"performedAtOrganization":{"_id":"670530","name":"Louisiana State University"}},{"role":{"_id":"138","name":"Principal Investigator"},"performedBy":{"_id":"51212","name":"Jennifer Beseres Pollack"},"performedAtOrganization":{"_id":"204","name":"Texas A&M, Corpus Christi"}},{"role":{"_id":"140","name":"Co-Principal Investigator"},"performedBy":{"_id":"764996","name":"Jerome La Peyre"},"performedAtOrganization":{"_id":"670530","name":"Louisiana State University"}},{"role":{"_id":"133","name":"Contact"},"performedBy":{"_id":"51212","name":"Jennifer Beseres Pollack"},"performedAtOrganization":{"_id":"204","name":"Texas A&M, Corpus Christi"}},{"role":{"_id":"135","name":"Technician"},"performedBy":{"_id":"870221","name":"Natasha Breaux"},"performedAtOrganization":{"_id":"204","name":"Texas A&M, Corpus Christi"}},{"role":{"_id":"137","name":"Data Manager"},"performedBy":{"_id":"805241","name":"Terry Palmer"},"performedAtOrganization":{"_id":"204","name":"Texas A&M, Corpus Christi"}},{"role":{"_id":"145","name":"BCO-DMO Data Manager"},"performedBy":{"_id":"748773","name":"Karen Soenen"},"performedAtOrganization":{"_id":"191","name":"Woods Hole Oceanographic Institution"}}],"deployments":[],"funding":[{"_id":"765008","name":"OCE-1737207","fundingSource":{"_id":"355","name":"NSF Division of Ocean Sciences"},"url":"https://www.nsf.gov/awardsearch/show-award?AWD_ID=1737207"}],"instruments":[],"parameters":[{"_id":"870598","name":"Trial","format":"","frictionlessType":"string","description":"

Experiment number: 1 or 2

","units":{"_id":"","name":"unitless"},"type":{"_id":"1759","name":"exp_id","description":"

Experiment Id

"}},{"_id":"870599","name":"Date","format":"%d%b%Y","frictionlessType":"date","description":"

Date (Central Daylight Time)

","units":{"_id":"","name":"unitless"},"type":{"_id":"1070","name":"date","description":"

date; generally reported in GMT as YYYYMMDD (year; month; day); also as MMDD (month; day); EqPac dates are local Hawaii time. ISO_Date format is YYYY-MM-DD (http://www.iso.org/iso/home/standards/iso8601.htm)

"}},{"_id":"870600","name":"Time","format":"%H:%M","frictionlessType":"time","description":"

Time (Central Daylight Time)

","units":{"_id":"","name":"unitless"},"type":{"_id":"1075","name":"time","description":"

Time GMT defined as HHMM; time of day; generally reported in GMT as HHMM (hours and minutes) but may also be HH.H (decimal hours) or HHMMSS (hours; minutes and seconds); in EqPac time is reported as Hawaii local time

"}},{"_id":"870601","name":"ISO_DateTime_UTC","format":"%Y-%m-%dT%H:%MZ","frictionlessType":"datetime","description":"

Sampling date and time in ISO format, UTC timezone

","units":{"_id":"","name":"unitless"},"type":{"_id":"1908","name":"ISO_DateTime_UTC","description":"

Date/Time (UTC) ISO formatted
\nThis standard is based on ISO 8601:2004(E) and takes on any of the following forms:
\n2009-08-30T09:05:00[.xx] (local time)
\n2009-08-30T14:05:00[.xx]Z (UTC time)
\n2009-08-30T14:05:00[.xx]-05:00
\nThe dashes and the colons can be dropped.
\nThe T can also be dropped \"by mutual agreement\", but one needs the trailing Z if the time is UTC.

Sample ISO_datetime_utc:
\n2009-08-30T14:05:00[.xx]Z (UTC time)

Format: YYYY-mm-ddTHH:MM:SS[.xx]Z (UTC time)

"}},{"_id":"870602","name":"Tank","format":"","frictionlessType":"integer","description":"

Tank

","units":{"_id":"","name":"unitless"},"type":{"_id":"1992","name":"tank","description":"

Tank Number or Tank Id. Used to identify tanks/containers used in various experiments

"}},{"_id":"870603","name":"Treatment","format":"","frictionlessType":"integer","description":"

Salinity Treatment (2, 4, 20, 38, 44)

","units":{"_id":"","name":"unitless"},"type":{"_id":"489066","name":"treatment","description":"

Experimental conditions applied to experimental units. In comparative experiments, members of the complementary group, the control group, receive either no treatment or a standard treatment.

"}},{"_id":"870604","name":"PC","format":"","frictionlessType":"integer","description":"

Shell height of Packery Channel Population

","units":{"_id":"","name":"millimeters (mm)"},"type":{"_id":"663027","name":"height","description":"

The measurement of an organism or object from base to top

"}},{"_id":"870605","name":"AB","format":"","frictionlessType":"integer","description":"

Shell height of Aransas Bay Population

","units":{"_id":"","name":"millimeters (mm)"},"type":{"_id":"663027","name":"height","description":"

The measurement of an organism or object from base to top

"}},{"_id":"870606","name":"VB","format":"","frictionlessType":"integer","description":"

Shell height of Vermillion Bay Population

","units":{"_id":"","name":"millimeters (mm)"},"type":{"_id":"663027","name":"height","description":"

The measurement of an organism or object from base to top

"}},{"_id":"870607","name":"CL","format":"","frictionlessType":"integer","description":"

Shell height of Calcasieu Lake Population

","units":{"_id":"","name":"millimeters (mm)"},"type":{"_id":"663027","name":"height","description":"

The measurement of an organism or object from base to top

"}},{"_id":"870608","name":"Comments","format":"","frictionlessType":"string","description":"

Comments

","units":{"_id":"","name":"unitless"},"type":{"_id":"778","name":"comment","description":"

free text comments, may only have meaning to submitting PI

"}}],"related_publications":[{"id":"870227","citation":"Marshall, D.A., S.M. Casas, W.C. Walton, F.S. Rikard, T.A. Palmer, N. Breaux, M.K. La Peyre, J. Beseres Pollack, M. Kelly and J.F. La Peyre, Divergence in salinity tolerance of northern Gulf of Mexico eastern oysters under field and laboratory exposure, Conservation Physiology, Volume 9, Issue 1, 2021, coab065, https://doi.org/10.1093/conphys/coab065","relation_type":["Results"],"relation_desc":""}],"related_datasets":[{"_id":"870316","name":"Salinity tolerance without acclimation: Water Quality","url":"","relation_type":"IsRelatedTo","type_desc":"

The dataset is related to another dataset.

\n","relation_desc":"Observations of same experiment.","citation":"Pollack, J. B., Palmer, T., Breaux, N., Kelly, M., La Peyre, J. (2023) Salinity tolerance of oysters without acclimation in lab conditions: mortality. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-02-24 doi:10.26008/1912/bco-dmo.870210.1"}],"services":[{"grouping":"","items":[{"name":"View Data","description":"An in-browser view of the primary tabular data file","url":"https://www.bco-dmo.org/dataset/870248/data/view","contentType":"text/html","actionType":"view"}]},{"grouping":"Metadata","items":[{"name":"HTML","description":"A simple HTML page of the dataset metadata","url":"https://www.bco-dmo.org/dataset/870248/description","contentType":"text/html","actionType":"view"},{"name":"PDF","description":"A PDF of the HTML dataset metadata","url":"https://www.bco-dmo.org/dataset/870248/Dataset_description.pdf","contentType":"application/pdf","actionType":"download"},{"name":"ISO 19115-2 (NOAA Profile)","description":"","url":"https://www.bco-dmo.org/dataset/870248/iso","contentType":"application/xml","actionType":"download"}]}],"programs":[],"projects":[{"_id":"764992","name":"Collaborative Research: Testing for local adaptation and responses to multiple stressors in populations of eastern oysters inhabiting a natural salinity gradient","acronym":"Oyster adaptation","description":"$1b"}],"archives":[{"id_value":"10.26008/1912/bco-dmo.870248.1","url":"https://doi.org/10.26008/1912/bco-dmo.870248.1","date_assigned":"2023-02-24","version_date":"2023-02-24","archiver":"Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)","archiver_id":"272"}],"license":{"name":"Creative Commons Attribution 4.0","id":"CC-BY-4.0","uri":"https://creativecommons.org/licenses/by/4.0/","spdxUri":"https://spdx.org/licenses/CC-BY-4.0"},"metrics":{"views":{"count":290},"downloads":{"count":354}}}}]