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            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset/870783.rdf" xlink:actuate="onRequest">Nutrient and metabolic fluxes on oyster reefs from the Choptank River and Harris Creek, Maryland data from June through August of 2017 (published in Jackson et al., 2018)</gmx:Anchor>
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            <gco:CharacterString>Cite this dataset as: Cornwell, J. (2022) Nutrient and metabolic fluxes on oyster reefs from the Choptank River and Harris Creek, Maryland data from June through August of 2017 (published in Jackson et al., 2018). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-03-14 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.870783.1 [access date]</gco:CharacterString>
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        <gco:CharacterString>Methods and Sampling: &amp;lt;p&amp;gt;Experiments were conducted in early and late summer (June and August) 2017 using the in situ equilibration of intact segments of oyster reef (hereafter “reef segments”) with ex situ incubation approach. As part of the larger project, reef segments were incubated first in the dark, then with illumination.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;After these incubations were complete, a subset of these samples (4 samples in June and 6 samples in August) was selected for additional study based upon whether the sample had at least one oyster over ~75 mm visible on the surface sediment. For each tray selected, the live oysters and oyster clumps were carefully removed from each tray, placed in clean and empty incubation chambers, aerated for ~1 h, and incubated in the dark. Solutes (NH4+, NO2/3-, SRP) and dissolved gases (O2, N2, Ar, DIC = [H2CO3]+[HCO3-]+[CO32-]) were collected approximately every 45 minutes (4 times total) during each set of incubations from a sampling tube fitted in the lid, while a water replacement tube pulled water from the water bath. Dissolved gases were preserved with 10μl of 50% saturated HgCl2, tightly sealed, submerged in water, and held at or slightly below incubation temperature until analysis. Solute samples were filtered through a 0.45 μm pore-size filter and kept frozen for analysis. N2, O2, and Ar concentrations were measured on a membrane-inlet mass spectrometer within 2 weeks of collection. DIC concentrations were measured using an infrared-based analyzer (Apollo SciTech). Phenol/hypochlorite colorimetry was used to determine NH4+ concentrations. NO2/3-was analyzed spectrophotometrically following Doane and Howarth, while a composite reagent of molybdic acid, ascorbic acid, and trivalent antimony were used to determine SRP concentrations.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Refer to Jackson et al. (2018) for complete methods.&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/686822.rdf" xlink:title="OCE-1427019" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1427019 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1427019</gmx:Anchor>
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&amp;lt;p&amp;gt;After these incubations were complete, a subset of these samples (4 samples in June and 6 samples in August) was selected for additional study based upon whether the sample had at least one oyster over ~75 mm visible on the surface sediment. For each tray selected, the live oysters and oyster clumps were carefully removed from each tray, placed in clean and empty incubation chambers, aerated for ~1 h, and incubated in the dark. Solutes (NH4+, NO2/3-, SRP) and dissolved gases (O2, N2, Ar, DIC = [H2CO3]+[HCO3-]+[CO32-]) were collected approximately every 45 minutes (4 times total) during each set of incubations from a sampling tube fitted in the lid, while a water replacement tube pulled water from the water bath. Dissolved gases were preserved with 10μl of 50% saturated HgCl2, tightly sealed, submerged in water, and held at or slightly below incubation temperature until analysis. Solute samples were filtered through a 0.45 μm pore-size filter and kept frozen for analysis. N2, O2, and Ar concentrations were measured on a membrane-inlet mass spectrometer within 2 weeks of collection. DIC concentrations were measured using an infrared-based analyzer (Apollo SciTech). Phenol/hypochlorite colorimetry was used to determine NH4+ concentrations. NO2/3-was analyzed spectrophotometrically following Doane and Howarth, while a composite reagent of molybdic acid, ascorbic acid, and trivalent antimony were used to determine SRP concentrations.&amp;lt;/p&amp;gt;

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    <gmd:MD_MaintenanceInformation>
      <gmd:maintenanceAndUpdateFrequency>
        <gmd:MD_MaintenanceFrequencyCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#MD_MaintenanceFrequencyCode" codeListValue="asNeeded" codeSpace="009">asNeeded</gmd:MD_MaintenanceFrequencyCode>
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		  <gmd:phone>
		    <gmd:CI_Telephone>
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				  </gmd:voice>
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				    <gco:CharacterString>508-289-2009</gco:CharacterString>
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		    <gmd:CI_Address>
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				    <gco:CharacterString>WHOI MS#36</gco:CharacterString>
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				  <gmd:city>
				    <gco:CharacterString>Woods Hole</gco:CharacterString>
				  </gmd:city>
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				    <gco:CharacterString>MA</gco:CharacterString>
				  </gmd:administrativeArea>
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				    <gco:CharacterString>USA</gco:CharacterString>
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				  </gmd:electronicMailAddress>
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		  </gmd:address>
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            <gmd:linkage>
              <gmd:URL>http://www.bco-dmo.org</gmd:URL>
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          </gmd:CI_OnlineResource>
        </gmd:onlineResource>
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      </gmd:hoursOfService>
		  <gmd:contactInstructions>
		    <gco:CharacterString>For questions regarding this resource, please contact BCO-DMO via the email address provided.</gco:CharacterString>
		  </gmd:contactInstructions>
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    <gmi:instrument>
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              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/768590.rdf" xlink:title="Apollo SciTech AS-C3 Dissolved Inorganic Carbon (DIC) analyzer" xlink:actuate="onRequest"></gmx:Anchor>
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            <gco:CharacterString>PI Supplied Instrument Name:  Instrument Name: Apollo SciTech AS-C3 Dissolved Inorganic Carbon (DIC) analyzer Instrument Short Name:Apollo SciTech AS-C3   Instrument Description: A Dissolved Inorganic Carbon (DIC) analyzer, for use in aquatic carbon dioxide parameter analysis of coastal waters, sediment pore-waters, and time-series incubation samples. The analyzer consists of a solid state infrared CO2 detector, a mass-flow controller, and a digital pump for transferring accurate amounts of reagent and sample. The analyzer uses an electronic cooling system to keep the reactor temperature below 3 degrees Celsius, and a Nafion dry tube to reduce the water vapour and keep the analyzer drift-free and maintenance-free for longer. The analyzer can handle sample volumes from 0.1 - 1.5 milliliters, however the best results are obtained from sample volumes between 0.5 - 1 milliliters. It takes approximately 3 minutes per analysis, and measurement precision is plus or minus 2 micromoles per kilogram or higher for surface seawater. It is designed for both land based and shipboard laboratory use.</gco:CharacterString>
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        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
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            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/661606.rdf" xlink:title="Membrane Inlet Mass Spectrometer" xlink:actuate="onRequest"></gmx:Anchor>
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            <gco:CharacterString>PI Supplied Instrument Name:  Instrument Name: Membrane Inlet Mass Spectrometer Instrument Short Name:MIMS   Instrument Description: Membrane-introduction mass spectrometry (MIMS) is a method of introducing analytes into the mass spectrometer's vacuum chamber via a semipermeable membrane.</gco:CharacterString>
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