http://lod.bco-dmo.org/id/dataset/872498 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2022-03-29 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Experimental tank parameters throughout the life of Clathromorphum compactum and C. nereostratum calcification experiment from from 2015-2016 (CorallineAlgaePaleo-pH project) 2022-04-04 publication 2022-04-04 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2022-04-19 publication https://doi.org/10.26008/1912/bco-dmo.872498.1 Justin B. Ries Northeastern University principalInvestigator Douglas B. Rasher Bigelow Laboratory for Ocean Sciences principalInvestigator Branwen Williams University of California-San Diego Scripps principalInvestigator Isaac Westfield Northeastern University principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Westfield, I., Ries, J. B., Williams, B., Rasher, D. B. (2022) Experimental tank parameters throughout the life of Clathromorphum compactum and C. nereostratum calcification experiment from from 2015-2016 (CorallineAlgaePaleo-pH project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-04-04 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.872498.1 [access date] Experimental tank parameters throughout the life of Clathromorphum compactum and C. nereostratum calcification experiment Dataset Description: Methods and Sampling: &lt;p&gt;&lt;strong&gt;Methodology:&amp;nbsp;&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Sampling and analytical procedures:&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;&lt;em&gt;C. compactum&lt;/em&gt; and &lt;em&gt;C. nereostratum&lt;/em&gt; cultured for 4 months under 12 pCO&lt;sub&gt;2&lt;/sub&gt;/T treatments in thirty-six, 42-liter aquaria.&lt;/p&gt; &lt;p&gt;Seawater samples were obtained every ~14 days using 250 mL ground-glass-stoppered borosilicate glass bottles for measurement of total alkalinity (TA) and dissolved inorganic carbon (DIC) and other carbonate parameters.&lt;/p&gt; &lt;p&gt;Temp, salinity, pH measured three times weekly.&lt;/p&gt; &lt;p&gt;For buoyant weight, specimens were suspended beneath a balance in an aquarium at 4 cm depth in seawater of constant temperature and salinity.&lt;/p&gt; &lt;p&gt;Specimens were dosed with calcein before the experiment and the vertical growth was determined by measuring the vertical growth from the resulting calcein line to the surface.&lt;/p&gt; Funding provided by NSF Arctic Sciences (NSF ARC) Award Number: PLR-1316141 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1316141 Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1459706 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1459706 Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1459827 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1459827 completed Justin B. Ries Northeastern University 781-581-7370 Northeastern University Marine Science Center 430 Nahant Rd. Nahant MA 01908 USA j.ries@northeastern.edu pointOfContact Douglas B. Rasher Bigelow Laboratory for Ocean Sciences 207-315-2567 60 Bigelow Drive East Boothbay ME 04544 USA drasher@bigelow.org pointOfContact Branwen Williams University of California-San Diego Scripps 909-607-8017 W.M. Keck Science Department 925 N. Mills Ave Claremont CA 91711 USA Branwen.Williams@ClaremontMcKenna.edu pointOfContact Isaac Westfield Northeastern University seymour47@gmail.com pointOfContact asNeeded Dataset Version: 1 Unknown Date Mean_pCO2 Mean_Temp Mean_pH Tank Group_ID Salinity Temp pCO2 pH VINDTA 3C (Marianda Corporation, Kiel, Germany) AccuFet™ Solid-State pH probe High precision partial-immersion glass thermometer YSI 3200 (Yellow Springs, Ohio, USA) theme None, User defined date Partial pressure of CO2 water temperature at measurement depth pH tank experiment id salinity featureType BCO-DMO Standard Parameters MARIANDA VINDTA 3C total inorganic carbon and titration alkalinity analyser pH Sensor Thermometer YSI Professional Plus Multi-Parameter Probe instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Collaborative Research: Development and application of a method using coralline algae to reconstruct past changes in pH and impacts on calcification https://osprey.bco-dmo.org/project/562825 Collaborative Research: Development and application of a method using coralline algae to reconstruct past changes in pH and impacts on calcification <p><em>Description from NSF award abstract:</em><br /> The impacts of recent and future human-caused increases in atmospheric CO2 on the acidity (pH) of shallow cold-water marine environments (a process known as "ocean acidification"), and on the organisms that inhabit them, are poorly understood. This is due, in part, to the difficulty in reconstructing past changes in ocean chemistry in these remote environments. This research seeks to develop and apply a technique to reconstruct past seawater pH from boron isotope signatures in long-lived crustose coralline alga that are widespread throughout shallow, cold-water marine environments. In addition, the research will evaluate the impact of changing seawater pH on the growth rate of these ecologically important organisms, which are thought to be particularly vulnerable to ocean acidification because of the high magnesium content of their skeleton. Overall, this project will advance understanding of ocean acidification in shallow, cold-water environments, and provide key information to evaluate the impact that changes in ocean pH have had on organisms inhabiting these environments. The outcomes of this work will provide important information to policy makers and legislators seeking to mitigate the negative effects of rising atmospheric CO2 on these fragile, high-latitude marine ecosystems.</p> <p>Funding supports a graduate student, numerous undergraduate researchers, and a new collaboration between two early career faculty members. Outreach includes mentoring high school students from groups underrepresented in the sciences through the Scripps College Academy and production of an educational film on the biological impacts of ocean acidification. The research team will strengthen international ties through collaboration with Canadian and UK scientists, while helping maintain US-based scientists at the forefront of this important sub-field of ocean acidification research.</p> <p>The work plan includes three main parts: (1) developing the first laboratory-derived and field-verified calibration of the delta11B-proxy of paleoseawater pH for coralline algae, (2) generating the first high-resolution, multi-centennial dataset of high-latitude seawater pH before (ca. 1365 to 1760 AD; i.e., "baseline") and after (ca. 1760 AD to present; i.e., "anthropogenic signal") the Industrial Revolution, and (3) evaluating the impact of anthropogenic ocean acidification on the linear extension, density, and ultrastructure of skeletons produced by an ecologically important, habitat-forming coralline red alga. The associated objectives are: (1) to provide a new tool for reconstructing paleo-seawater pH, (2) to generate historical records of ocean acidification that would elucidate the rate and magnitude of high-latitude ocean acidification that could be used to verify predictive models, and (3) to establish empirical relationships between ocean acidification and coralline algal calcification that would inform predictions of future impacts of ocean acidification on high-latitude marine calcifiers.</p> <p><em>Additional information may be found on the following lab websites:</em><br /> Ries Lab - <a href="http://nuweb2.neu.edu/rieslab/" target="_blank">http://nuweb2.neu.edu/rieslab/</a><br /> Williams Marine Environmental Change (MEC) Lab - <a href="https://branwenwilliams.com/" target="_blank">https://branwenwilliams.com/</a></p> CorallineAlgaePaleo-pH largerWorkCitation project eng; USA oceans 2015-10-08 2016-02-09 Marine Science Center, Northeastern University; and Keck Science Department, Claremont Colleges 0 BCO-DMO catalogue of parameters from Experimental tank parameters throughout the life of Clathromorphum compactum and C. nereostratum calcification experiment from from 2015-2016 (CorallineAlgaePaleo-pH project) Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/873277.rdf Name: Date Units: unitless Description: <p>Date of sampling measurement</p> http://lod.bco-dmo.org/id/dataset-parameter/873278.rdf Name: Mean_pCO2 Units: uatm Description: <p>Mean pCO2 of experimental tank</p> http://lod.bco-dmo.org/id/dataset-parameter/873279.rdf Name: Mean_Temp Units: degrees Celsius Description: <p>Mean temperature of experimental tank</p> http://lod.bco-dmo.org/id/dataset-parameter/873280.rdf Name: Mean_pH Units: unitless Description: <p>Mean pH of experimental tank</p> http://lod.bco-dmo.org/id/dataset-parameter/873281.rdf Name: Tank Units: unitless Description: <p>Tank number within a temperature/pCO2 combination</p> http://lod.bco-dmo.org/id/dataset-parameter/873282.rdf Name: Group_ID Units: unitless Description: <p>Cominations of experimental conditions and Tank number used to create a unique identifier for groups within the experiment</p> http://lod.bco-dmo.org/id/dataset-parameter/873283.rdf Name: Salinity Units: psu Description: <p>Salinity of the experimental tank</p> http://lod.bco-dmo.org/id/dataset-parameter/873284.rdf Name: Temp Units: degrees Celsius Description: <p>Temperature of the experimental tank</p> http://lod.bco-dmo.org/id/dataset-parameter/873285.rdf Name: pCO2 Units: uatm Description: <p>pCO2 of the experimental tank</p> http://lod.bco-dmo.org/id/dataset-parameter/873286.rdf Name: pH Units: unitless Description: <p>pH of the experimental tank</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 100137 https://darchive.mblwhoilibrary.org/bitstream/1912/28607/1/dataset-872498_measured-experimental-tank-parameters__v1.tsv download https://doi.org/10.26008/1912/bco-dmo.872498.1 download onLine dataset &lt;p&gt;&lt;strong&gt;Methodology:&amp;nbsp;&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Sampling and analytical procedures:&lt;/strong&gt;&lt;/p&gt; &lt;p&gt;&lt;em&gt;C. compactum&lt;/em&gt; and &lt;em&gt;C. nereostratum&lt;/em&gt; cultured for 4 months under 12 pCO&lt;sub&gt;2&lt;/sub&gt;/T treatments in thirty-six, 42-liter aquaria.&lt;/p&gt; &lt;p&gt;Seawater samples were obtained every ~14 days using 250 mL ground-glass-stoppered borosilicate glass bottles for measurement of total alkalinity (TA) and dissolved inorganic carbon (DIC) and other carbonate parameters.&lt;/p&gt; &lt;p&gt;Temp, salinity, pH measured three times weekly.&lt;/p&gt; &lt;p&gt;For buoyant weight, specimens were suspended beneath a balance in an aquarium at 4 cm depth in seawater of constant temperature and salinity.&lt;/p&gt; &lt;p&gt;Specimens were dosed with calcein before the experiment and the vertical growth was determined by measuring the vertical growth from the resulting calcein line to the surface.&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;CO2Sys used to calculate all carbonate parameters, including pCO&lt;sub&gt;2.&lt;/sub&gt;&lt;/p&gt; Specified by the Principal Investigator(s) asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact VINDTA 3C (Marianda Corporation, Kiel, Germany) VINDTA 3C (Marianda Corporation, Kiel, Germany) PI Supplied Instrument Name: VINDTA 3C (Marianda Corporation, Kiel, Germany) PI Supplied Instrument Description:Measures total alkalinity and DIC using closed-cell potentiometric Gran titration and coulometry (UIC 5400), with both methods calibrated using certified Dickson DIC/TA standards. Instrument Name: MARIANDA VINDTA 3C total inorganic carbon and titration alkalinity analyser Instrument Short Name:inorganic carbon and alkalinity analyser Instrument Description: The Versatile INstrument for the Determination of Total inorganic carbon and titration Alkalinity (VINDTA) 3C is a laboratory alkalinity titration system combined with an extraction unit for coulometric titration, which simultaneously determines the alkalinity and dissolved inorganic carbon content of a sample. The sample transport is performed with peristaltic pumps and acid is added to the sample using a membrane pump. No pressurizing system is required and only one gas supply (nitrogen or dry and CO2-free air) is necessary. The system uses a Metrohm Titrino 719S, an ORION-Ross pH electrode and a Metrohm reference electrode. The burette, the pipette and the analysis cell have a water jacket around them. Precision is typically +/- 1 umol/kg for TA and/or DIC in open ocean water. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0481/ AccuFet™ Solid-State pH probe AccuFet™ Solid-State pH probe PI Supplied Instrument Name: AccuFet™ Solid-State pH probe PI Supplied Instrument Description:AccuFet™ Solid-State pH probe, calibrated with 7.00 and 10.01 NBS buffers (for slope of calibration) and a Dickson Lab DIC/TA standard (for y-intercept of calibration). Instrument Name: pH Sensor Instrument Short Name:pH Sensor Instrument Description: An instrument that measures the hydrogen ion activity in solutions. The overall concentration of hydrogen ions is inversely related to its pH.  The pH scale ranges from 0 to 14 and indicates whether acidic (more H+) or basic (less H+).  High precision partial-immersion glass thermometer High precision partial-immersion glass thermometer PI Supplied Instrument Name: High precision partial-immersion glass thermometer PI Supplied Instrument Description:High precision partial-immersion glass thermometer (precision ±0.3%; accuracy ±0.4%). Instrument Name: Thermometer Instrument Short Name: Instrument Description: A device designed to measure temperature. YSI 3200 (Yellow Springs, Ohio, USA) YSI 3200 (Yellow Springs, Ohio, USA) PI Supplied Instrument Name: YSI 3200 (Yellow Springs, Ohio, USA) PI Supplied Instrument Description:YSI 3200 (Yellow Springs, Ohio, USA) conductivity probe with a cell constant of k = 10. Instrument Name: YSI Professional Plus Multi-Parameter Probe Instrument Short Name:YSI ProPlus Instrument Description: The YSI Professional Plus handheld multiparameter meter provides for the measurement of a variety of combinations for dissolved oxygen, conductivity, specific conductance, salinity, resistivity, total dissolved solids (TDS), pH, ORP, pH/ORP combination, ammonium (ammonia), nitrate, chloride and temperature. More information from the manufacturer.